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1. 发明申请

US20130129707A1 ANTIMICROBIAL ACTIVITY ENHANCING AGENT 审中-公开
标题翻译：抗菌活性增强剂
公开(公告)号：US20130129707A1
公开(公告)日：2013-05-23
申请号：US13813240
申请日：2011-07-29
申请人： Mai Murata , Hiroyuki Wakabayashi , Koji Yamauchi
发明人： Mai Murata , Hiroyuki Wakabayashi , Koji Yamauchi
IPC分类号： A01N63/02
CPC分类号： A01N63/02 , A23K10/14 , A23K20/147 , A23K50/10 , A23K50/40 , A23K50/80 , A23L2/66 , A23L3/3526 , A23L33/19 , A23V2002/00 , A61K35/20 , A23V2200/10 , A23V2250/54248 , A23V2300/02 , A23V2300/30
摘要： An antimicrobial activity enhancing agent for lactoferrin or a lactoferrin hydrolysate, which contains a milk protein as an active ingredient and has the following properties, and a method for producing the antimicrobial activity enhancing agent, which comprises (a) the step of contacting raw milk with a cation exchange resin to adsorb proteins in the raw milk to the cation exchange resin, (b) the step of washing the cation exchange resin having undergone the adsorption treatment, and flowing an elution solvent to elute a protein fraction having an isoelectric point of 9.0 to 11.0, and (c) the step of subjecting the eluted protein fraction to membrane filtration using an ultrafiltration membrane having a molecular weight cutoff of 30 kDa to prepare the milk protein having the following properties:(A) an action of enhancing the antimicrobial activity of lactoferrin or a lactoferrin hydrolysate, (B) a molecular weight of 30 kDa or less, (C) an isoelectric point of 9.0 to 11.0.
摘要翻译：一种含有乳蛋白作为活性成分并具有以下性质的乳铁蛋白或乳铁蛋白水解产物的抗微生物活性增强剂及其制备方法，其包括：（a）将生牛奶与阳离子交换树脂，用于将原料乳中的蛋白质吸附到阳离子交换树脂中，（b）洗涤经过吸附处理的阳离子交换树脂，并使洗脱溶剂流动，洗脱等电点为9.0的蛋白质级分至11.0，和（c）使用分子量截留值为30kDa的超滤膜对洗脱的蛋白质级分进行膜过滤的步骤，以制备具有以下性质的乳蛋白：（A）增强抗微生物活性的作用的乳铁蛋白或乳铁蛋白水解物，（B）分子量为30kDa以下，（C）等电点为9.0〜11.0。

2. 发明申请

US20140134704A1 Antimicrobial Activity Enhancing Agent 审中-公开
标题翻译：抗菌活性增强剂
公开(公告)号：US20140134704A1
公开(公告)日：2014-05-15
申请号：US14001570
申请日：2011-07-29
申请人： Mai Murata , Hiroyuki Wakabayashi , Koji Yamauchi
发明人： Mai Murata , Hiroyuki Wakabayashi , Koji Yamauchi
IPC分类号： C12N9/22
CPC分类号： A01N63/00 , A23K20/147 , A23K20/189 , A23L29/06 , A23L33/18 , A23L33/19 , A61K38/17 , C12N9/22 , C12Y301/27 , Y02A50/473
摘要： An antimicrobial activity enhancing agent for lactoferrin or a lactoferrin hydrolysate, which contains ribonuclease-4 as an active ingredient, and a method for producing an antimicrobial activity enhancing agent for lactoferrin or a lactoferrin hydrolysate, which comprises (a) the step of contacting raw milk with a cation exchange resin to adsorb proteins in the raw milk to the cation exchange resin, (b) the step of washing the cation exchange resin having undergone the adsorption treatment, and flowing an elution solvent to elute a protein fraction having an isoelectric point of 8.0 to 10.0, and (c) the step of subjecting the eluted protein fraction to gel filtration using a gel filtration carrier to collect a fraction of a molecular weight of 30 kDa or less.
摘要翻译：一种含有核糖核酸酶-4作为活性成分的乳铁蛋白或乳铁蛋白水解物的抗微生物活性增强剂及其制备用于乳铁蛋白或乳铁蛋白水解产物的抗微生物活性增强剂的方法，其特征在于，（a）与阳离子交换树脂一起吸附生乳中的蛋白质至阳离子交换树脂，（b）洗涤已经进行吸附处理的阳离子交换树脂，并且使洗脱溶剂流动以洗脱等电点的蛋白质级分 8.0至10.0，和（c）使用凝胶过滤载体对洗脱的蛋白质级分进行凝胶过滤以收集分子量为30kDa或更低的级分的步骤。

3. 发明授权

US08420128B2 Method of imparting heat resistance to lactoferrin 有权
标题翻译：赋予乳铁蛋白耐热性的方法
公开(公告)号：US08420128B2
公开(公告)日：2013-04-16
申请号：US12867979
申请日：2009-03-31
申请人： Ayako Horigome , Mai Murata , Koji Yamauchi , Mitsunori Takase , Yasuhiro Takeda , Junichi Hashimoto , Ikumi Kojima
发明人： Ayako Horigome , Mai Murata , Koji Yamauchi , Mitsunori Takase , Yasuhiro Takeda , Junichi Hashimoto , Ikumi Kojima
IPC分类号： A01N59/20 , A61K33/34
CPC分类号： A61K47/42 , A23L33/19 , A61K9/0019 , A61K9/0095 , A61K9/08 , A61K9/2063 , A61K9/4866 , A61K38/40 , A61K47/26
摘要： An agent for thermally stabilizing lactoferrin, which comprises a nucleic acid as an active ingredient, can be added to lactoferrin to impart thermal stability to lactoferrin. Thermally stabilized lactoferrin can be heat-sterilized at a pH value around a neutral pH value while keeping its activity.
摘要翻译：可以将含有核酸作为活性成分的用于热稳定的乳铁蛋白的试剂加入到乳铁蛋白中以赋予乳铁蛋白热稳定性。热稳定的乳铁蛋白可以在pH值约为中性pH值的同时保持其活性而被热灭菌。

4. 发明申请

US20100310673A1 SUBSTANCE AND COMPOSITION BOTH CAPABLE OF IMPARTING HEAT RESISTANCE 有权
标题翻译：具有耐腐蚀性的两种物质和组合物
公开(公告)号：US20100310673A1
公开(公告)日：2010-12-09
申请号：US12867979
申请日：2009-03-31
申请人： Ayako Horigome , Mai Murata , Koji Yamauchi , Mitsunori Takase , Yasuhiro Takeda , Junichi Hashimoto , Ikumi Kojima
发明人： Ayako Horigome , Mai Murata , Koji Yamauchi , Mitsunori Takase , Yasuhiro Takeda , Junichi Hashimoto , Ikumi Kojima
IPC分类号： A61K33/34 , C07H21/00 , A61K33/26 , A61K33/30 , A61K38/40 , C07K14/79 , A61P31/04 , A61P3/00 , A61P37/04 , A61P29/00 , A23L1/305
CPC分类号： A61K47/42 , A23L33/19 , A61K9/0019 , A61K9/0095 , A61K9/08 , A61K9/2063 , A61K9/4866 , A61K38/40 , A61K47/26
摘要： An agent for thermally stabilizing lactoferrin, which comprises a nucleic acid as an active ingredient, can be added to lactoferrin to impart thermal stability to lactoferrin. Thermally stabilized lactoferrin can be heat-sterilized at a pH value around a neutral pH value while keeping its activity.
摘要翻译：可以将含有核酸作为活性成分的用于热稳定的乳铁蛋白的试剂加入到乳铁蛋白中以赋予乳铁蛋白热稳定性。热稳定的乳铁蛋白可以在pH值约为中性pH值的同时保持其活性而被热灭菌。

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