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1. 外观设计

USD970746S1 Stage for cell cryopreservation operation 有权
公开(公告)号：USD970746S1
公开(公告)日：2022-11-22
申请号：US29764097
申请日：2020-12-28
申请人： MITSUBISHI PAPER MILLS LIMITED
设计人： Atsushi Matsuzawa

2. 发明授权

US10412958B2 Device for cell or tissue cryopreservation by vitrification 有权
公开(公告)号：US10412958B2
公开(公告)日：2019-09-17
申请号：US15025571
申请日：2014-10-17
申请人： SCHOOL JURIDICAL PERSON KITASATO INSTITUTE , MITSUBISHI PAPER MILLS LIMITED
发明人： Kenji Momozawa , Atsushi Matsuzawa , Katsumitsu Susaki
IPC分类号： C12M3/00 , A01N1/00 , A01N1/02 , C12M1/00
摘要： Provided is a device for vitrification-based cryopreservation which enables easy and reliable vitrification freezing of cells or tissues. The device of the present invention for cell or tissue cryopreservation by vitrification has a porous structure made of a material having a refractive index of 1.45 or less as a vitrification solution absorber.

3. 发明授权

US11510408B2 Cryopreservation jig for cryopreserving cells or tissues 有权
公开(公告)号：US11510408B2
公开(公告)日：2022-11-29
申请号：US16621337
申请日：2018-06-08
申请人： MITSUBISHI PAPER MILLS LIMITED
发明人： Kakeru Yoshida , Atsushi Matsuzawa , Yukio Tokunaga
IPC分类号： A01N1/02
摘要： The present invention aims to provide a device for cryopreservation which enables easy and reliable cryopreservation of a cell or tissue. The device for cryopreservation of a cell or tissue of the present invention includes a deposition part on which a cell or tissue is to be deposited together with a preservation solution, wherein a surface of the deposition part includes a protrusion for holding a cell or tissue and a recess for storing a preservation solution.

4. 发明申请

US20220361483A1 FIXTURE OF CRYOPRESERVATION JIG AND FREEZING AND THAWING METHOD USING SAID FIXTURE 有权
公开(公告)号：US20220361483A1
公开(公告)日：2022-11-17
申请号：US17618373
申请日：2020-06-03
申请人： MITSUBISHI PAPER MILLS LIMITED
发明人： Atsushi Matsuzawa , Kakeru Yoshida
IPC分类号： A01N1/02 , C12M1/00
摘要： Provided is a cryopreservation jig fixture including: a base having an upper surface, side surfaces, and a bottom surface; and either a slit on the upper surface of the base or an insertion portion for inserting a cryopreservation jig on the upper surface of the base and a slit having one end opened at a lateral portion of the insertion portion. Also provided is a method of freezing and thawing a cell or tissue, which uses the fixture.

5. 发明申请

US20210235687A1 CRYOPRESERVATION SOLUTION AND CRYOPRESERVATION METHOD 有权
公开(公告)号：US20210235687A1
公开(公告)日：2021-08-05
申请号：US17050722
申请日：2019-04-02
申请人： MITSUBISHI PAPER MILLS LIMITED
发明人： Atsushi Matsuzawa
IPC分类号： A01N1/02
摘要： The present invention relates to a cryopreservation solution containing polyvinyl alcohol having a saponification degree of 84 mol % or lower.

6. 发明申请

US20210298291A1 AUXILIARY DEVICE FOR CRYOPRESERVATION 有权
公开(公告)号：US20210298291A1
公开(公告)日：2021-09-30
申请号：US17212298
申请日：2021-03-25
申请人： MITSUBISHI PAPER MILLS LIMITED
发明人： Atsushi Matsuzawa
IPC分类号： A01N1/02
摘要： The present invention relates to an auxiliary device for cryopreservation that is capable of stably holding a device for cryopreservation, facilitates coverage of a cell or tissue deposited on a strip with a cap without allowing the cap to come into contact with the cell or tissue, and enables quick immersion of the device for cryopreservation in liquid nitrogen. The present invention relates specifically to an auxiliary device for cryopreservation, including: a substrate; a cap holder; an observation section having an aperture structure; and a device body holder, the cap holder, the observation section having an aperture structure, and the device body holder being arranged in the substrate in a substantially straight line.

7. 发明授权

US10010070B2 Device for vitrification preservation of cells or tissues 有权
公开(公告)号：US10010070B2
公开(公告)日：2018-07-03
申请号：US15322849
申请日：2015-06-01
申请人： MITSUBISHI PAPER MILLS LIMITED
发明人： Atsushi Matsuzawa , Katsumitsu Susaki
IPC分类号： A01N1/02
摘要： The present invention aims to provide a device for vitrification cryopreservation enabling easy and reliable vitrification cryopreservation of a cell or tissue. The device for vitrification cryopreservation of a cell or tissue of the present invention includes a support and a vitrification solution absorber including at least an adhesive layer and a vitrification solution absorbing layer in the given order on the support. The device includes a portion with no adhesive layer disposed between the support and a portion of the vitrification solution absorbing layer configured to hold a cell or tissue.

8. 发明申请

US20170135335A1 DEVICE FOR VITRIFICATION PRESERVATION OF CELLS OR TISSUES 审中-公开
公开(公告)号：US20170135335A1
公开(公告)日：2017-05-18
申请号：US15322849
申请日：2015-06-01
申请人： MITSUBISHI PAPER MILLS LIMITED
发明人： Atsushi Matsuzawa , Katsumitsu Susaki
IPC分类号： A01N1/02
CPC分类号： A01N1/0252 , A01N1/02 , A01N1/0221 , A01N1/0268 , C12M1/00 , C12M3/00 , C12N5/00
摘要： The present invention aims to provide a device for vitrification cryopreservation enabling easy and reliable vitrification cryopreservation of a cell or tissue. The device for vitrification cryopreservation of a cell or tissue of the present invention includes a support and a vitrification solution absorber including at least an adhesive layer and a vitrification solution absorbing layer in the given order on the support. The device includes a portion with no adhesive layer disposed between the support and a portion of the vitrification solution absorbing layer configured to hold a cell or tissue.

9. 发明申请

US20160235056A1 DEVICE FOR CELL OR TISSUE CRYOPRESERVATION BY VITRIFICATION 审中-公开
标题翻译：用于细胞或组织的装置通过维修进行CRYOPRESERVATION
公开(公告)号：US20160235056A1
公开(公告)日：2016-08-18
申请号：US15025571
申请日：2014-10-17
申请人： SCHOOL JURIDICAL PERSON KITASATO INSTITUTE , MITSUBISHI PAPER MILLS LIMITED
发明人： Kenji Momozawa , Atsushi Matsuzawa , Katsumitsu Susaki
IPC分类号： A01N1/02
CPC分类号： A01N1/0268 , C12M45/22
摘要： Provided is a device for vitrification-based cryopreservation which enables easy and reliable vitrification freezing of cells or tissues. The device of the present invention for cell or tissue cryopreservation by vitrification has a porous structure made of a material having a refractive index of 1.45 or less as a vitrification solution absorber.
摘要翻译：提供了一种用于基于玻璃化的冷冻保存装置，其能够容易且可靠地玻璃化冷冻细胞或组织。用于通过玻璃化进行细胞或组织冷冻保存的本发明的装置具有由折射率为1.45以下的材料制成的多孔结构作为玻璃化溶液吸收体。

10. 发明申请

US20150247118A1 METHOD FOR PRODUCING 3D CELL CULTURE 审中-公开
标题翻译：用于生产3D细胞培养的方法
公开(公告)号：US20150247118A1
公开(公告)日：2015-09-03
申请号：US14427536
申请日：2013-09-12
申请人： OSAKA UNIVERSITY , MITSUBISHI PAPER MILLS LIMITED
发明人： Mitsuru Akashi , Michiya Matsusaki , Atsushi Matsuzawa
IPC分类号： C12N5/00
CPC分类号： C12N5/0062 , C12N5/0671 , C12N2533/52 , C12N2533/90
摘要： Provided is a novel method for producing a three-dimensional cell culture construct. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; culturing the seeded coated cells in a culture medium; and using at least a portion of the culture medium continuously for 5 days or more. A method for producing a three-dimensional cell culture construct including at least two or more laminated cell layers includes the following: seeding coated cells in which a cell surface is coated with a coating film containing an extracellular matrix component; and culturing the seeded coated cells in a culture medium. The culture process of the coated cells includes culturing the coated cells in 70 μL or more of the culture medium per 1×104 cells.
摘要翻译：提供了一种用于生产三维细胞培养构建体的新方法。包含至少两层以上的层叠单元层的三维细胞培养构建体的制造方法包括以下步骤：将含有细胞外基质成分的涂膜对细胞表面进行接种的涂覆细胞，在培养基中培养接种的包被细胞; 并将培养基的至少一部分连续使用5天以上。包含至少两层以上的层叠单元层的三维细胞培养构建体的制造方法包括以下步骤：将含有细胞外基质成分的涂膜对细胞表面进行接种的涂覆细胞，并在培养基中培养接种的包被的细胞。包被细胞的培养过程包括每1×10 4个细胞培养70μL以上的培养基。

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