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1. 发明授权

US6074625A Boron-containing hormone analogs and methods of their use in imaging or killing cells having hormone receptors 失效
标题翻译：含硼激素类似物及其用于成像或杀死具有激素受体的细胞的方法
公开(公告)号：US6074625A
公开(公告)日：2000-06-13
申请号：US808203
申请日：1997-02-28
申请人： M. Frederick Hawthorne , Mark T. Groudine
发明人： M. Frederick Hawthorne , Mark T. Groudine
IPC分类号： G01N33/53 , A61K31/69 , A61K41/00 , A61K47/48 , A61K49/00 , C07F5/02 , G01N33/566 , A61K51/00 , A61M36/14
CPC分类号： A61K41/0095
摘要： Boron containing compounds are targeted to hormonally responsive cells by contacting the cells with a conjugate of a boron containing moiety conjugated to a ligand having binding specificity for an intracellular receptor of the cell. The conjugates are useful for imaging or killing hormonally responsive cells, such as hormonally responsive tumor cells. For target cell killing, the cells are contacted with a .sup.10 B containing moiety conjugated to a ligand having binding specificity for an intracellular hormone receptor of the cells. The .sup.10 B containing moiety becomes associated with the hormone receptor of the cells, which may then be irradiated with neutrons to kill the cells by boron neutron capture. For target cell imaging, the cells are contacted with a .sup.11 B containing moiety conjugated to a ligand having binding specificity for an intracellular hormone receptor of the cells, and the boron that becomes associated with the hormone receptor of the cells may then be imaged using magnetic resonance imaging techniques.
摘要翻译：含硼化合物通过使细胞与与细胞的细胞内受体具有结合特异性的配体缀合的含硼部分的缀合物接触来靶向激素反应性细胞。缀合物可用于成像或杀死激素反应性细胞，例如激素反应性肿瘤细胞。对于靶细胞杀伤，细胞与含有与具有对细胞的细胞内激素受体具有结合特异性的配体缀合的含10B部分接触。含有10B的部分与细胞的激素受体相关联，然后可以用中子照射以通过硼中子捕获来杀死细胞。对于靶细胞成像，将细胞与含有与对细胞的细胞内激素受体具有结合特异性的配体缀合的含有11B的部分接触，然后使用磁共振成像与细胞激素受体相关的硼成像技术。

2. 发明授权

US5543319A Recombination-proficient avian/mammalian microcell hybrids 失效
标题翻译：重组熟练的禽/哺乳动物微细胞杂交
公开(公告)号：US5543319A
公开(公告)日：1996-08-06
申请号：US415354
申请日：1995-03-31
申请人： R. E. Keith Fournier , Mark T. Groudine , Ellen S. Dieken , Elliot M. Epner
发明人： R. E. Keith Fournier , Mark T. Groudine , Ellen S. Dieken , Elliot M. Epner
IPC分类号： C12N5/0781 , C12N5/12 , C12N5/26 , C12N15/08
CPC分类号： C12N5/0635 , C12N2510/00
摘要： An avian/mammalian microcell hybrid immortalized pre B cell line containing a mammalian chromosome that carries a selectable marker has been produced. The avian/mammalian microcell hybrid immortalized pre B cell line can be used for introducing predetermined point mutations into specific mammalian chromosomal loci via high frequency homologous recombination.
摘要翻译：已经生产了含有哺乳动物染色体携带选择性标记的禽/哺乳动物微细胞杂交永生化B细胞系。禽/哺乳动物微细胞杂交永生化前B细胞系可用于通过高频同源重组将预定点突变引入特定哺乳动物染色体基因座。

3. 发明授权

US5866338A Cell cycle checkpoint genes 失效
标题翻译：细胞周期检查点基因
公开(公告)号：US5866338A
公开(公告)日：1999-02-02
申请号：US870693
申请日：1997-06-06
申请人： Leland H. Hartwell , Ted A. Weinert , Sharon E. Plon , Mark T. Groudine
发明人： Leland H. Hartwell , Ted A. Weinert , Sharon E. Plon , Mark T. Groudine
IPC分类号： C07K14/47 , C12Q1/68 , G01N33/50
CPC分类号： C07K14/47 , C12Q1/6876 , G01N33/5011 , C12Q2600/158
摘要： Human checkpoint huCDC34, huRAD9.sub.compA, and huRAD9.sub.compB cDNAs shown in FIGS. 1, 2, and 3. A method for isolating a human checkpoint cDNA that is capable of restoring growth at a restrictive temperature in a yeast test cell, wherein the yeast test cell comprises a genome having a first gene that forms a DNA strand break at a restrictive temperature and a second gene that fails to induce a cell cycle arrest in response to the DNA strand break, whereby the growth of the yeast test cell is inhibited at the restrictive temperature, the method comprising the steps of: obtaining a human cDNA library comprising a plurality of human cDNA clones; inserting the human cDNA clones individually into plasmid vectors comprising a selectable marker gene; transforming a culture of the yeast test cells with the plasmid vectors from the preceding step; selecting for yeast test cells transformed with the selectable marker gene; growing the selected transformants at the restrictive temperature and isolating a candidate transformant capable of growing at the restrictive temperature; and identifying the human cDNA carried by the candidate transformant as a human checkpoint cDNA by sequencing the human cDNA carried by the candidate transformant and determining that the human cDNA is less than 50% homologous with both the first gene and the second gene. Also yeast checkpoint RAD17, RAD24, MEC1, MEC2, and MEC3 cDNAs shown in FIGS. 4-8.
摘要翻译：人体检查点huCDC34，huRAD9compA和huRAD9compB cDNA，如图1和2所示。 1，2和3.一种用于分离能够在酵母测试细胞中在限制性温度恢复生长的人类检查点cDNA的方法，其中酵母测试细胞包含具有形成DNA链断裂的第一个基因的基因组限制性温度和第二基因，其不能诱导响应于DNA链断裂的细胞周期停滞，由此在限制性温度下抑制酵母测试细胞的生长，该方法包括以下步骤：获得人cDNA文库包括多个人cDNA克隆; 将人cDNA克隆单独插入包含选择标记基因的质粒载体中; 用前述步骤的质粒载体转化酵母测试细胞的培养物; 选择用可选择标记基因转化的酵母测试细胞; 在限制性温度下生长所选择的转化体并分离能够在限制温度下生长的候选转化体; 以及通过对候选转化体携带的人cDNA进行测序并确定人cDNA与第一基因和第二基因两者都小于50％同源性来鉴定候选转化体携带的人cDNA作为人检测点cDNA。还有图1和2所示的酵母检查点RAD17，RAD24，MEC1，MEC2和MEC3 cDNA。 4-8。

4. 发明授权

US5674996A Cell cycle checkpoint genes 失效
标题翻译：细胞周期检查点基因
公开(公告)号：US5674996A
公开(公告)日：1997-10-07
申请号：US198446
申请日：1994-02-18
申请人： Leland H. Hartwell , Ted A. Weinert , Sharon E. Plon , Mark T. Groudine
发明人： Leland H. Hartwell , Ted A. Weinert , Sharon E. Plon , Mark T. Groudine
IPC分类号： C07K14/47 , C12Q1/68 , G01N33/50 , C07H21/04
CPC分类号： C07K14/47 , C12Q1/6876 , G01N33/5011 , C12Q2600/158
摘要： Human checkpoint huCDC34, huRAD9.sub.compA, and huRAD9.sub.compB cDNAs shown in SEQ ID Nos:7-9. A method for isolating a human checkpoint cDNA that is capable of restoring growth at a restrictive temperature in a yeast test cell, wherein the yeast test cell comprises a genome having a first gene that forms a DNA strand break at a restrictive temperature and a second gene that fails to induce a cell cycle arrest in response to the DNA strand break, whereby the growth of the yeast test cell is inhibited at the restrictive temperature, the method comprising the steps of: obtaining a human cDNA library comprising a plurality of human cDNA clones; inserting the human cDNA clones individually into plasmid vectors comprising a selectable marker gene; transforming a culture of the yeast test cells with the plasmid vectors from the preceding step; selecting for yeast test cells transformed with the selectable marker gene; growing the selected transformants at the restrictive temperature and isolating a candidate transformant capable of growing at the restrictive temperature; and identifying the human cDNA carried by the candidate transformant as a human checkpoint cDNA by sequencing the human cDNA carried by the candidate transformant and determining that the human cDNA is less than 50% homologous with both the first gene and the second gene. Also yeast checkpoint RAD17, RAD24, MEC1, MEC2, and MEC3 cDNAs shown in SEQ ID Nos:10-19.
摘要翻译：人类检查点huCDC34，huRAD9compA和huRAD9compB cDNA，如SEQ ID NO：7-9所示。一种用于分离能够在酵母测试细胞中以限制性温度恢复生长的人类检查点cDNA的方法，其中所述酵母测试细胞包含具有在限制性温度下形成DNA链断裂的第一基因的基因组和第二基因其不能响应于DNA链断裂诱导细胞周期停滞，由此酵母测试细胞的生长在限制性温度下被抑制，该方法包括以下步骤：获得包含多个人cDNA克隆的人cDNA文库 ; 将人cDNA克隆单独插入包含选择标记基因的质粒载体中; 用前述步骤的质粒载体转化酵母测试细胞的培养物; 选择用可选择标记基因转化的酵母测试细胞; 在限制性温度下生长所选择的转化体并分离能够在限制温度下生长的候选转化体; 以及通过对候选转化体携带的人cDNA进行测序并确定人cDNA与第一基因和第二基因两者都小于50％同源性来鉴定候选转化体携带的人cDNA作为人检测点cDNA。还有SEQ ID NO：10-19所示的酵母检查点RAD17，RAD24，MEC1，MEC2和MEC3 cDNA。

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