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1. 发明授权

US08288109B2 Procedure for structural characterization of a recombinant polyclonal protein or a polyclonal cell line 有权
标题翻译：重组多克隆蛋白或多克隆细胞系的结构表征方法
公开(公告)号：US08288109B2
公开(公告)日：2012-10-16
申请号：US11658021
申请日：2005-07-20
申请人： Lone Kjaer Rasmussen , Torben Frandsen , Soren Kofoed Rasmussen , Peter Sejer Andersen
发明人： Lone Kjaer Rasmussen , Torben Frandsen , Soren Kofoed Rasmussen , Peter Sejer Andersen
IPC分类号： C40B40/10 , C40B50/06
CPC分类号： C12Q1/6876 , G01N30/96 , G01N33/6803
摘要： The present invention provides a structural characterization platform that can be used to assess the stability of a polyclonal cell line during production, as well as batch-to-batch consistency of the final polyclonal products. The structural characterization platform is based on genetic analyses as well as protein characterization techniques that alone or in combination provide the necessary information to characterize the polyclonal cell line and final products. The collection of different homologous proteins to be analyzed with the platform techniques if for example a recombinant polyclonal antibody or a mixture of monoclonal antibodies.
摘要翻译：本发明提供了可用于评估生产过程中多克隆细胞系的稳定性以及最终多克隆产物的批次间一致性的结构表征平台。结构表征平台基于遗传分析以及蛋白质表征技术，其单独或组合提供了表征多克隆细胞系和最终产物的必要信息。使用平台技术收集不同的同源蛋白质，例如重组多克隆抗体或单克隆抗体的混合物。

2. 发明申请

US20080131882A1 Procedure for Structural Characterization of a Recombinant Polylonal Protein or a Polyclonal Cell Line 有权
标题翻译：重组多克隆蛋白或多克隆细胞系的结构表征方法
公开(公告)号：US20080131882A1
公开(公告)日：2008-06-05
申请号：US11658021
申请日：2005-07-20
申请人： Lone Kjaer Rasmussen , Torben Frandsen , Soren Kofoed Rasmussen , Peter Sejer Andersen
发明人： Lone Kjaer Rasmussen , Torben Frandsen , Soren Kofoed Rasmussen , Peter Sejer Andersen
IPC分类号： C12Q1/68 , G01N33/53 , C07K16/00
CPC分类号： C12Q1/6876 , G01N30/96 , G01N33/6803
摘要： The present invention provides a structural characterization platform that can be used to assess the stability of a polyclonal cell line during production, as well as batch-to-batch consistency of the final polyclonal products. The structural characterization platform is based on genetic analyses as well as protein characterization techniques that alone or in combination provide the necessary information to characterize the polyclonal cell line and final products. The collection of different homologous proteins to be analyzed with the platform techniques if for example a recombinant polyclonal antibody or a mixture of monoclonal antibodies.
摘要翻译：本发明提供了可用于评估生产过程中多克隆细胞系的稳定性以及最终多克隆产物的批次间一致性的结构表征平台。结构表征平台基于遗传分析以及蛋白质表征技术，其单独或组合提供了表征多克隆细胞系和最终产物的必要信息。使用平台技术收集不同的同源蛋白质，例如重组多克隆抗体或单克隆抗体的混合物。

3. 发明申请

US20070031928A1 Glucoamylase variants 失效
标题翻译：葡糖淀粉酶变体
公开(公告)号：US20070031928A1
公开(公告)日：2007-02-08
申请号：US11524693
申请日：2006-09-21
申请人： Bjarne Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Hendriksen , Torben Frandsen
发明人： Bjarne Nielsen , Allan Svendsen , Henrik Pedersen , Jesper Vind , Hanne Hendriksen , Torben Frandsen
IPC分类号： C12P21/06 , C12P19/02 , C07H21/04 , C12P7/06 , C12N9/32 , C12N15/74 , C12N1/16
CPC分类号： C12N9/2428 , C12P19/14 , C12P19/20 , Y02E50/17
摘要： The invention relates to a variant of a parent fungal glucoamylase, which exhibits improved thermal stability and/or increased specific activity using saccharide substrates.
摘要翻译：本发明涉及亲本真菌葡糖淀粉酶的变体，其使用糖基底显示出改进的热稳定性和/或增加的比活性。

4. 发明申请

US20050202533A1 Maltogenic alpha-amylase variants 审中-公开
公开(公告)号：US20050202533A1
公开(公告)日：2005-09-15
申请号：US11037573
申请日：2005-01-18
申请人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Frandsen
发明人： Joel Cherry , Allan Svendsen , Carsten Andersen , Lars Beier , Torben Frandsen
IPC分类号： A23L7/10 , A21D8/04 , C12N9/28 , C12N9/32 , C07H21/04 , C12N1/21 , C12N15/74
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.

5. 发明申请

US20050250181A1 Cell-wall degrading enzyme variants 审中-公开
标题翻译：细胞壁降解酶变体
公开(公告)号：US20050250181A1
公开(公告)日：2005-11-10
申请号：US10926709
申请日：2004-08-26
申请人： Sanne Schroder Glad , Carsten Andersen , Martin Schulein , Hanne Dela , Torben Frandsen
发明人： Sanne Schroder Glad , Carsten Andersen , Martin Schulein , Hanne Dela , Torben Frandsen
IPC分类号： C07H21/04 , C11D3/386 , C11D7/42 , C12N1/21 , C12N9/18 , C12N9/88 , C12N15/74
CPC分类号： C11D3/38636 , C12N9/88
摘要： The present invention relates to variants of a cell-wall degrading enzyme having a beta-helix structure, which variant has at least one substituent in a position determined by identifying all residues potentially belonging to a stack; characterizing the stack as interior or exterior; characterizing the stack as polar, hydrophobic or aromatic/heteroaromatic based on the dominating characteristics of the parent or wild-type enzyme stack residues and/or its orientation relative to the beta-helix (interior or exterior); optimizing all stack positions of a stack either to hydrophobic aliphatic amino acids, hydrophobic aromatic or polar amino acids by allowing mutations within one or all positions to amino acids belonging to one of these groups; measuring thermostability of the variants by DSC or an application-related assay such as a Pad-Steam application test; and selecting the stabilized variants. Variants of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, D141 or E141, D145, K165, R194 and R199 when aligned with the pectate lyase comprising the amino acid sequence of SEQ ID NO: 2 are preferred.
摘要翻译：本发明涉及具有β-螺旋结构的细胞壁降解酶的变体，该变体具有至少一个取代基，其位置通过鉴定潜在属于堆叠的所有残基而确定; 将堆栈表征为内部或外部; 基于母体或野生型酶堆残基的主导特征和/或其相对于β-螺旋（内部或外部）的取向，将所述叠层表征为极性，疏水性或芳族/杂芳族化合物; 通过在属于这些基团之一的氨基酸的一个或所有位置内允许突变将堆叠的所有堆叠位置优化为疏水性脂族氨基酸，疏水性芳族或极性氨基酸; 通过DSC测量变体的热稳定性或应用相关测定，例如Pad-Steam应用测试; 并选择稳定的变体。当与包含SEQ ID NO：1的氨基酸序列的果胶酸裂合酶比对时，具有保守氨基酸残基D111，D141或E141，D145，K165，R194和R199的野生型亲本果胶酸裂解酶（EC 4.2.2.2） 2是优选的。

6. 发明申请

US20050009068A1 Lysophospholipase 审中-公开
标题翻译：溶血磷脂酶
公开(公告)号：US20050009068A1
公开(公告)日：2005-01-13
申请号：US10849106
申请日：2004-05-19
申请人： Hiroaki Udagawa , Torben Frandsen , Tom Nielsen , Markus Kauppinen , Soren Christensen
发明人： Hiroaki Udagawa , Torben Frandsen , Tom Nielsen , Markus Kauppinen , Soren Christensen
IPC分类号： C12N9/18 , C12Q1/68 , C07H21/04 , C12N1/16 , C12N9/20 , C12N15/74
CPC分类号： C12Y301/01005 , C12N9/18
摘要： The inventors have isolated lysophospholipases from Aspergillus (A. niger and A. oryzae) having molecular masses of about 68 kDa and amino acid sequences of 600-604 amino acid residues. The novel lysophospholipases have only a limited homology to known amino acid sequences. The inventors also isolated genes encoding the novel enzymes and cloned them into E. coli strains.
摘要翻译：本发明人具有分子量为约68kDa和氨基酸序列为600-604个氨基酸残基的曲霉（A.niger和米曲霉）的分离的溶血磷脂酶。新型溶血磷脂酶与已知的氨基酸序列仅具有有限的同源性。本发明人还分离了编码新型酶的基因并将其克隆到大肠杆菌菌株中。

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