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1. 发明授权

US09200299B2 Genetically engineered cell encoding ole amino acid motifs in a carbon source producing aliphatic ketones or olefins 有权
标题翻译：编码在产生脂族酮或烯烃的碳源中的烯基氨基酸基序的遗传工程改造的细胞
公开(公告)号：US09200299B2
公开(公告)日：2015-12-01
申请号：US12278962
申请日：2008-05-20
申请人： Lisa Friedman , Bernardo da Costa
发明人： Lisa Friedman , Bernardo da Costa
IPC分类号： C12N15/85 , C12P7/64 , C10G3/00 , C10L1/06 , C10L1/08 , C12N9/02 , C12N9/10
CPC分类号： C12P5/026 , C10G3/00 , C10G2300/1014 , C10G2300/1018 , C10G2400/02 , C10G2400/04 , C10G2400/08 , C10G2400/22 , C10L1/04 , C10L1/06 , C10L1/08 , C10L2200/0469 , C10L2270/023 , C10L2270/026 , C10L2270/04 , C10L2290/26 , C12N9/0004 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12P5/02 , C12P7/26 , C12P7/649 , C12Q1/02 , C12Y301/02 , C12Y402/03 , C12Y602/01003 , Y02E50/13 , Y02P30/20
摘要： The invention provides isolated nucleic acids and isolated polypeptides involved in the synthesis of hydrocarbons and hydrocarbon intermediates. Homologs of, conservative variants of, and sequences having at least about 35% sequence identity with nucleic acids involved in the synthesis of hydrocarbons and hydrocarbon intermediates are also provided. The invention further provides methods for producing an aliphatic ketone or a hydrocarbon, as well as a method for identifying an enzyme useful for the production of hydrocarbons.
摘要翻译：本发明提供分离的核酸和参与合成烃和烃中间体的分离的多肽。还提供了与合成烃和烃中间体的核酸具有至少约35％序列同一性的保守变体和序列的同源物。本发明还提供了生产脂族酮或烃的方法，以及用于鉴定可用于生产烃的酶的方法。

2. 发明申请

US20100235934A1 HYDROCARBON-PRODUCING GENES AND METHODS OF THEIR USE 有权
标题翻译：油料生产基因及其使用方法
公开(公告)号：US20100235934A1
公开(公告)日：2010-09-16
申请号：US12278962
申请日：2008-05-20
申请人： Lisa Friedman , Bernardo da Costa
发明人： Lisa Friedman , Bernardo da Costa
IPC分类号： C12P5/00 , C07H21/04 , C12N15/63 , C12N5/10 , C12N1/15 , C12N1/21 , C12N1/13 , C12N1/19 , C07C11/00 , C07K14/21 , A01K67/027
CPC分类号： C12P5/026 , C10G3/00 , C10G2300/1014 , C10G2300/1018 , C10G2400/02 , C10G2400/04 , C10G2400/08 , C10G2400/22 , C10L1/04 , C10L1/06 , C10L1/08 , C10L2200/0469 , C10L2270/023 , C10L2270/026 , C10L2270/04 , C10L2290/26 , C12N9/0004 , C12N9/1029 , C12N9/16 , C12N9/88 , C12N9/93 , C12P5/02 , C12P7/26 , C12P7/649 , C12Q1/02 , C12Y301/02 , C12Y402/03 , C12Y602/01003 , Y02E50/13 , Y02P30/20
摘要： The invention provides isolated nucleic acids and isolated polypeptides involved in the synthesis of hydrocarbons and hydrocarbon intermediates. Homologs of, conservative variants of, and sequences having at least about 35% sequence identity with nucleic acids involved in the synthesis of hydrocarbons and hydrocarbon intermediates are also provided. The invention further provides methods for producing an aliphatic ketone or a hydrocarbon, as well as a method for identifying an enzyme useful for the production of hydrocarbons.
摘要翻译：本发明提供分离的核酸和参与合成烃和烃中间体的分离的多肽。还提供了与合成烃和烃中间体的核酸具有至少约35％序列同一性的保守变体和序列的同源物。本发明还提供了生产脂族酮或烃的方法，以及用于鉴定可用于生产烃的酶的方法。

3. 发明授权

US4585011A Conjugate eye movement detector for sleep analysis 失效
标题翻译：用于睡眠分析的共轭眼动检测器
公开(公告)号：US4585011A
公开(公告)日：1986-04-29
申请号：US659297
申请日：1984-10-10
申请人： Roger Broughton , Bernardo da Costa
发明人： Roger Broughton , Bernardo da Costa
IPC分类号： A61B5/0496 , A61B5/04
CPC分类号： A61B5/0496
摘要： The eye movement detector detects events occurring during the electro-oculogram (EOG) signal in which components of the signal having a predetermined rise time, are coincident with components of the same signal having a predetermined amplitude.
摘要翻译：眼动检测器检测在具有预定上升时间的信号的分量与具有预定幅度的相同信号的分量一致的电眼图（EOG）信号期间发生的事件。

4. 发明授权

US4550736A Movement artifact detector for sleep analysis 失效
标题翻译：运动伪影检测器进行睡眠分析
公开(公告)号：US4550736A
公开(公告)日：1985-11-05
申请号：US659295
申请日：1984-10-10
申请人： Roger Broughton , Bernardo da Costa
发明人： Roger Broughton , Bernardo da Costa
IPC分类号： A61B5/0476 , G06F17/00 , A61B5/04
CPC分类号： A61B5/0476
摘要： The movement detector monitors the electroencephalogram (EEG) signal in order to detect components having a real-time frequency greater than 30 Hz and an amplitude greater than 18 .mu.V; a real-time frequency greater than 30 Hz and an amplitude between .+-.9 .mu.V; or a real-time frequency less than 1.2 Hz and an amplitude greater than 200 .mu.V. An output signal is provided when at least one of these components is detected.
摘要翻译：移动检测器监测脑电图（EEG）信号，以便检测具有大于30Hz的实时频率和大于18μV的幅度的分量; 实时频率大于30Hz，振幅为+/-9μV; 或小于1.2Hz的实时频率和大于200μV的幅度。当检测到这些组件中的至少一个时，提供输出信号。

5. 发明申请

US20080293060A1 Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes 审中-公开
标题翻译：用于鉴定烃反应，运输和生物合成基因的方法和组合物
公开(公告)号：US20080293060A1
公开(公告)日：2008-11-27
申请号：US12108278
申请日：2008-04-23
申请人： Andreas Schirmer , Zhihao Hu , Bernardo da Costa
发明人： Andreas Schirmer , Zhihao Hu , Bernardo da Costa
IPC分类号： C12Q1/68 , C12N1/21
CPC分类号： C12Q1/6897 , C12N15/1086
摘要： Disclosed is a method using an alkane response element (ARE) from, e.g., Acinetobacter spp. to (i) identify and clone hydrocarbon biosynthesis genes, (ii) identify and clone hydrocarbon transporter genes (iii) identify and clone hydrocarbon response genes. Screening cells were developed that expressed a transcriptional activator, e.g., alkR, and included a reporter gene, e.g., GFP operatively linked to an ARE promoter, e.g., the alkM promoter. The cells were transformed with libraries from organisms capable of hydrocarbon biosynthesis. Transformed cells that expressed the reporter gene harbored library-derived genes involved in one or more of the above-mentioned processes; and these genes were isolated from the cells using standard molecular biology techniques. Additional systems were designed wherein screening cells also expressed a gene identified in the original screen, e.g., an additional hydrocarbon pathway gene, e.g., an enhancer.
摘要翻译：公开了一种使用来自例如不动杆菌属（Acinetobacter spp）的烷烃应答元件（ARE）的方法。（i）识别和克隆烃类生物合成基因，（ii）鉴定和克隆烃转运蛋白基因（iii）鉴定和克隆烃类反应基因。开发了表达转录激活物例如alkR的筛选细胞，并且包括报告基因，例如可操作地连接到ARE启动子的例如alkM启动子的GFP。用能够进行烃生物合成的生物体的文库转化细胞。表达报告基因的转化细胞携带参与一个或多个上述过程的文库衍生基因; 并使用标准分子生物学技术从细胞中分离出这些基因。设计另外的系统，其中筛选细胞还表达在原始筛选中鉴定的基因，例如另外的烃途径基因，例如增强子。

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