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1. 发明授权

US6020538A Genetic transformation of orchids 失效
标题翻译：兰花遗传转化
公开(公告)号：US6020538A
公开(公告)日：2000-02-01
申请号：US71204
申请日：1998-05-01
申请人： Kyung-Hwan Han , Jaemo Yang
发明人： Kyung-Hwan Han , Jaemo Yang
IPC分类号： C12N15/31 , C12N15/82 , C12N15/54 , C12N15/65
CPC分类号： C12N15/8207
摘要： A method is disclosed for making genetically engineered orchid species using accelerated particle transformation. Meristem-tip tissue of orchid is cultured into a protocorm-like body (PLB). PLB is meristematic tissue and can be induced to regenerate into whole plant. In order to achieve efficient transformation it is necessary that relatively large numbers of meristematic cells are bombarded with the DNA-coated particles. After a particle-mediated transformation event, it is also important to stimulate continued meristemoid development before and during antibiotic selection and to reduce toxic effect of oxidized products of phenolics exuded after the wounding. It has been discovered that by using the techniques described in the present invention the meristematic tissue development, before and after the bombardment, can be stimulated while the toxic effect of oxidized phenolics can be reduced, resulting in successful transformation. The bombarded PLBs are multiplied and selected for the presence of gene products coded by the introduced genes. Shoot regeneration is then induced from the PLBs and transgenic seedlings are produced. Preferred plants for carrying out the present invention are the members of the genus Cymbidium.
摘要翻译：公开了一种使用加速粒子转化来制造转基因植物兰花物种的方法。兰花的分生组织培养成原生质体样体（PLB）。 PLB是分生组织，可诱导再生成全植株。为了实现有效的转化，需要用DNA涂覆的颗粒轰击相对大量的分生细胞。在粒子介导的转化事件之后，在抗生素选择之前和期间刺激持续的分生组织发育以及减少伤口后渗出的酚类氧化产物的毒性作用也是重要的。已经发现，通过使用本发明中描述的技术，可以刺激在轰击之前和之后的分生组织发育，同时可以减少氧化酚类的毒性作用，导致成功的转化。将被轰击的PLB乘以并选择由引入的基因编码的基因产物的存在。然后从PLB诱导射击再生，并产生转基因幼苗。用于实施本发明的优选植物是蕙兰属的成员。

2. 发明授权

US09845478B2 Compositions and methods for xylem-specific expression in plant cells 有权
公开(公告)号：US09845478B2
公开(公告)日：2017-12-19
申请号：US13821095
申请日：2011-09-13
申请人： Kyung-Hwan Han , Jae-Heung Ko
发明人： Kyung-Hwan Han , Jae-Heung Ko
IPC分类号： C12N15/113 , C12N15/82 , C07K14/415
CPC分类号： C12N15/8223 , C07K14/415 , C12N15/113 , C12N15/82 , C12N15/8216 , C12N15/8226 , C12N15/8227 , C12N15/8246 , C12N15/8255
摘要： The invention provides promoter sequences that regulate specific expression of operably linked sequences in developing xylem cells and/or in developing xylem tissue. The developing xylem-specific sequences are exemplified by the DX5, DX8, DX11, and DX15 promoters, portions thereof, and homologs thereof. The invention further provides expression vectors, cells, tissues and plants that contain the invention's sequences. The compositions of the invention and methods of using them are useful in, for example, improving the quantity (biomass) and/or the quality (wood density, lignin content, sugar content etc.) of expressed biomass feedstock products that may be used for bioenergy, biorefinary, and generating wood products such as pulp, paper, and solid wood.

3. 发明申请

US20150052641A1 CONTROL OF CELLULOSE BIOSYNTHESIS BY OVEREXPRESSION OF A TRANSCRIPTION FACTOR 有权
标题翻译：通过转录因子的过表达控制纤维素生物合成
公开(公告)号：US20150052641A1
公开(公告)日：2015-02-19
申请号：US14381040
申请日：2013-02-26
申请人： Kyung-Hwan Han , Jae-Heung Ko , Won-Chan Kim , Joo-Yeol Kim
发明人： Kyung-Hwan Han , Jae-Heung Ko , Won-Chan Kim , Joo-Yeol Kim
IPC分类号： C12N15/82
CPC分类号： C12N15/8246 , A01H5/00
摘要： The invention relates to the over-expression of a transcription factor selected from the group consisting of MYB46, HAM1, HAM2, MYB112, WRKY11, ERF6, and any combination thereof in a plant, which can modulate and thereby modulating the cellulose content of the plant.
摘要翻译：本发明涉及在植物中选自MYB46，HAM1，HAM2，MYB112，WRKY11，ERF6及其任何组合的转录因子的过表达，其可以调节并由此调节植物的纤维素含量。

4. 发明授权

US09371539B2 DNA encoding ring zinc-finger protein and the use of the DNA in vectors and bacteria and in plants 有权
标题翻译：编码环锌指蛋白的DNA和在载体和细菌和植物中使用DNA
公开(公告)号：US09371539B2
公开(公告)日：2016-06-21
申请号：US13180953
申请日：2011-07-12
申请人： Kyung-Hwan Han , Jae-Heung Ko
发明人： Kyung-Hwan Han , Jae-Heung Ko
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8273 , C07K14/415
摘要： The present inventions relate to compositions and methods for providing stress tolerant transgenic plants comprising a RING domain zinc-finger motif transcription factor protein. More particularly, the invention relates to compositions and methods comprising a RING-H2 domain transcription factor protein for providing drought and salt tolerant plants, in particular comprising a recombinant XERICO gene and protein.
摘要翻译：本发明涉及提供包含RING结构域锌指基序转录因子蛋白质的胁迫耐受性转基因植物的组合物和方法。更具体地，本发明涉及包含用于提供干旱和耐盐植物的RING-H2结构域转录因子蛋白质的组合物和方法，特别是包含重组XERICO基因和蛋白质。

5. 发明申请

US20120011615A1 DNA Encoding Ring Zinc-Finger Protein and the use of the DNA in Vectors and Bacteria and in Plants 有权
标题翻译：编码环锌指蛋白的DNA和载体和细菌和植物中的DNA的使用
公开(公告)号：US20120011615A1
公开(公告)日：2012-01-12
申请号：US13180953
申请日：2011-07-12
申请人： Kyung-Hwan Han , Jae-Heung Ko
发明人： Kyung-Hwan Han , Jae-Heung Ko
IPC分类号： A01H1/00 , C08B31/00 , C07K14/415 , A01H5/00 , C12P19/04 , C12P21/00 , C12N15/82 , C07C53/126 , C12P7/64
CPC分类号： C12N15/8273 , C07K14/415
摘要： The present inventions relate to compositions and methods for providing stress tolerant transgenic plants comprising a RING domain zinc-finger motif transcription factor protein. More particularly, the invention relates to compositions and methods comprising a RING-H2 domain transcription factor protein for providing drought and salt tolerant plants, in particular comprising a recombinant XERICO gene and protein.
摘要翻译：本发明涉及提供包含RING结构域锌指基序转录因子蛋白质的胁迫耐受性转基因植物的组合物和方法。更具体地，本发明涉及包含用于提供干旱和耐盐植物的RING-H2结构域转录因子蛋白质的组合物和方法，特别是包含重组XERICO基因和蛋白质。

6. 发明授权

US06235860B1 Natural rubber product from fig tree and production method thereof 失效
标题翻译：无花果树天然橡胶制品及其生产方法
公开(公告)号：US06235860B1
公开(公告)日：2001-05-22
申请号：US09322639
申请日：1999-05-28
申请人： Hun-Seung Kang , Kyung-Hwan Han
发明人： Hun-Seung Kang , Kyung-Hwan Han
IPC分类号： C08G8300
CPC分类号： C08C1/00 , Y10S528/931
摘要： Natural rubber products are made from fig tree (Ficus carica) by homogenizing the plants in an acetone/benzene and separating the rubber-containing phase by centrifugation. F. carica contains about 4% (w/v) natural rubber in latex, and 0.3, 0.1 and 0.1 % in bark, leaf and fruit, respectively. Molecular mass of the natural rubber from F. carica is about 128 kD. The latex serum of F. carica contains only a small numbers of proteins, with a 48 kD and a 25 kD protein as major proteins. Similar to Hevea brasiliensis and Parthenium argentatum Gray, proteins participating in rubber biosynthesis of F. carica are tightly associated with the rubber particles. Natural rubber is made using the latex serum and the proteins in latex, leaf, bark, and fruit of F. carica.
摘要翻译：天然橡胶制品由无花果树（Ficus carica）制成，通过在丙酮/苯中均匀化植物，并通过离心分离含橡胶的相。 car ica分别含有胶乳中约4％（w / v）的天然橡胶，分别含有0.3,0.1和0.1％的树皮，叶子和水果。来自樟树的天然橡胶的分子质量约为128kD。 F.carica的乳胶血清只含有少量蛋白质，以48kD和25kD蛋白为主要蛋白质。类似于三叶草（Brassiliensis）和金缕梅（Gray），参与橡胶生物合成的蛋白质与橡胶颗粒紧密相关。天然橡胶使用胶乳血清和乳胶，叶，树皮和果实中的蛋白质制成。

7. 发明申请

US20130254930A1 COMPOSITIONS AND METHODS FOR XYLEM-SPECIFIC EXPRESSION IN PLANT CELLS 有权
标题翻译：植物细胞中XYLEM特异性表达的组合物和方法
公开(公告)号：US20130254930A1
公开(公告)日：2013-09-26
申请号：US13821095
申请日：2011-09-13
申请人： Kyung-Hwan Han , Jae-Heung Ko
发明人： Kyung-Hwan Han , Jae-Heung Ko
IPC分类号： C12N15/82
CPC分类号： C12N15/8223 , C07K14/415 , C12N15/113 , C12N15/82 , C12N15/8216 , C12N15/8226 , C12N15/8227 , C12N15/8246 , C12N15/8255
摘要： The invention provides promoter sequences that regulate specific expression of operably linked sequences in developing xylem cells and/or in developing xylem tissue. The developing xylem-specific sequences are exemplified by the DX5, DX8, DX11, and DX15 promoters, portions thereof, and homologs thereof. The invention further provides expression vectors, cells, tissues and plants that contain the invention's sequences. The compositions of the invention and methods of using them are useful in, for example, improving the quantity (biomass) and/or the quality (wood density, lignin content, sugar content etc.) of expressed biomass feedstock products that may be used for bioenergy, biorefinary, and generating wood products such as pulp, paper, and solid wood.
摘要翻译：本发明提供启动子序列，其调控显影木质部细胞和/或显影木质部组织中可操作地连接的序列的特异性表达。显影的木质部特异性序列由DX5，DX8，DX11和DX15启动子，其部分及其同源物举例说明。本发明进一步提供含有本发明序列的表达载体，细胞，组织和植物。本发明的组合物及其使用方法可用于例如改进表达的生物质原料产品的数量（生物量）和/或质量（木材密度，木质素含量，糖含量等），其可用于生物能源，生物废水和生产木制品，如纸浆，纸和实木。

8. 发明申请

US20080034449A1 DNA encoding ring zinc-finger protein and the use of the DNA in vectors and bacteria and in plants 有权
标题翻译：编码环锌指蛋白的DNA和在载体和细菌和植物中使用DNA
公开(公告)号：US20080034449A1
公开(公告)日：2008-02-07
申请号：US11484947
申请日：2006-07-12
申请人： Kyung-Hwan Han , Jae-Heung Ko
发明人： Kyung-Hwan Han , Jae-Heung Ko
IPC分类号： C12N15/09 , A01H5/00 , C12N15/00
CPC分类号： C12N15/8273 , C07K14/415
摘要： The present inventions relate to compositions and methods for providing stress tolerant transgenic plants comprising a RING domain zinc-finger motif transcription factor protein. More particularly, the invention relates to compositions and methods comprising a RING-H2 domain transcription factor protein for providing drought and salt tolerant plants, in particular comprising a recombinant XERICO gene and protein.
摘要翻译：本发明涉及提供包含RING结构域锌指基序转录因子蛋白质的胁迫耐受性转基因植物的组合物和方法。更具体地，本发明涉及包含用于提供干旱和耐盐植物的RING-H2结构域转录因子蛋白质的组合物和方法，特别是包含重组XERICO基因和蛋白质。

9. 发明申请

US20150225737A1 Drought-Tolerance in Plants 审中-公开
标题翻译：植物耐旱性
公开(公告)号：US20150225737A1
公开(公告)日：2015-08-13
申请号：US14617061
申请日：2015-02-09
申请人： Kyung-Hwan Han , Won-Chan Kim , Joo-Yeol Kim , Jae-Heung Ko
发明人： Kyung-Hwan Han , Won-Chan Kim , Joo-Yeol Kim , Jae-Heung Ko
IPC分类号： C12N15/82
CPC分类号： C12N15/8273 , C12N15/113 , C12N15/8237
摘要： Drought-inducible plant promoters are described herein that are useful for expressing drought tolerance factors in plants.
摘要翻译：本文描述了可用于在植物中表达耐旱因子的干旱诱导型植物启动子。

10. 发明授权

US07977535B2 DNA encoding ring zinc-finger protein and the use of the DNA in vectors and bacteria and in plants 有权
标题翻译：编码环锌指蛋白的DNA和在载体和细菌和植物中使用DNA
公开(公告)号：US07977535B2
公开(公告)日：2011-07-12
申请号：US11484947
申请日：2006-07-12
申请人： Kyung-Hwan Han , Jae-Heung Ko
发明人： Kyung-Hwan Han , Jae-Heung Ko
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8273 , C07K14/415
摘要： The present inventions relate to compositions and methods for providing stress tolerant transgenic plants comprising a RING domain zinc-finger motif transcription factor protein. More particularly, the invention relates to compositions and methods comprising a RING-H2 domain transcription factor protein for providing drought and salt tolerant plants, in particular comprising a recombinant XERICO gene and protein.
摘要翻译：本发明涉及提供包含RING结构域锌指基序转录因子蛋白质的胁迫耐受性转基因植物的组合物和方法。更具体地，本发明涉及包含用于提供干旱和耐盐植物的RING-H2结构域转录因子蛋白质的组合物和方法，特别是包含重组XERICO基因和蛋白质。

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