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    • 7. 发明授权
    • Mutant cells of Pichia
    • 毕赤酵母突变体细胞
    • US06316245B1
    • 2001-11-13
    • US09383384
    • 1999-08-26
    • Sang Yong KimDeok Kun OhSoo Ryun Jung
    • Sang Yong KimDeok Kun OhSoo Ryun Jung
    • C12N116
    • C12R1/84C12P7/18Y10S435/938
    • The present invention relates to a fermentation process to have a high productivity with a novel mutant of Pichia sp., more specifically, for preparing erythritol under optimal fermentation conditions for maximum erythritol production by optimizing the environmental conditions of culture such as pH, temperature and by controlling osmotic pressure. A two-stage fermentation was performed to control osmotic pressure. Osmotic pressure was adjusted to a low level during growth phase and to a relatively high level during production phase by adding continuously glucose and NaCl or KCl. Therefore, erythritol production could be increased.
    • 本发明涉及一种利用毕赤酵母的新型突变体具有高生产率的发酵方法,更具体地说,用于通过优化培养物的环境条件如pH,温度和通过以下方式在最佳发酵条件下制备赤藓糖醇来最大化赤藓糖醇生产: 控制渗透压。 进行两阶段发酵以控制渗透压。 通过连续添加葡萄糖和NaCl或KCl,在生长阶段将渗透压调节至低水平,并在生产阶段调节至较高水平。 因此,可以增加赤藓糖醇的生产。