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1. 发明申请

US20060073566A1 Active modified hedgehog proteins 审中-公开
标题翻译：活性修饰的刺猬蛋白
公开(公告)号：US20060073566A1
公开(公告)日：2006-04-06
申请号：US11273989
申请日：2005-11-15
申请人： Kurt Lang , Ulrike Leser , Tilmann Seytter
发明人： Kurt Lang , Ulrike Leser , Tilmann Seytter
IPC分类号： C12P21/06 , C07H21/04 , C12N5/06 , C07K14/47
CPC分类号： C07K14/46 , A61K38/00 , C07K2319/02 , C12N2799/026
摘要： The present invention provides isolated highly active hedgehog proteins esterified with a fatty acid having from 14 to 20 carbon atoms at the N-terminal domain of the protein. The highly active hh proteins are particularly useful therapeutic agents for treating bone disorders and neurodegenerative diseases. Methods for obtaining the highly active modified hedgehog proteins are also provided.
摘要翻译：本发明提供了在蛋白质的N-末端结构域上用具有14至20个碳原子的脂肪酸酯化的分离的高活性刺猬蛋白。高活性的hh蛋白质是治疗骨骼疾病和神经变性疾病的特别有用的治疗剂。还提供了获得高活性修饰的刺猬蛋白的方法。

2. 发明授权

US07153836B2 Active hedgehog protein conjugate 失效
公开(公告)号：US07153836B2
公开(公告)日：2006-12-26
申请号：US10961982
申请日：2004-10-08
申请人： Angelika Esswein , Kurt Lang , Petra Rueger , Tilmann Seytter
发明人： Angelika Esswein , Kurt Lang , Petra Rueger , Tilmann Seytter
IPC分类号： A61K38/17 , C07K1/113 , C07K14/47
CPC分类号： A61K31/185 , A61K31/727 , A61K38/00 , C07K14/47
摘要： A hedgehog conjugate which is characterized in that it contains: a) a polypeptide composed of 10 to 30 hydrophobic amino acids and/or amino acids which form transmembrane helices and are positively charged, b) 1 to 4 aliphatic, saturated or unsaturated hydrocarbon residues with a chain length of 10 to 24 C atoms and with a hydrophobic action or c) a hydrophobic thio compound covalently bound to a hedgehog protein and which has a several-fold increased activity and is suitable as a pharmaceutical agent.

3. 发明授权

US06994990B1 Active modified hedgehog proteins 失效
公开(公告)号：US06994990B1
公开(公告)日：2006-02-07
申请号：US09198975
申请日：1998-11-24
申请人： Kurt Lang , Ulrike Leser , Tilman Seytter
发明人： Kurt Lang , Ulrike Leser , Tilman Seytter
IPC分类号： C12P21/06
CPC分类号： C07K14/46 , A61K38/00 , C07K2319/02 , C12N2799/026
摘要： The present invention provides isolated highly active hedgehog proteins esterified with a fatty acid having from 14 to 20 carbon atoms at the N-terminal domain of the protein. The highly active hh proteins are particularly useful therapeutic agents for treating bone disorders and neurodegenerative diseases. Methods for obtaining the highly active modified hedgehog proteins are also provided.

4. 发明授权

US5888967A Use of plasminogen activators to stimulate local bone growth 失效
标题翻译：使用纤溶酶原激活剂刺激局部骨生长
公开(公告)号：US5888967A
公开(公告)日：1999-03-30
申请号：US492
申请日：1998-02-09
申请人： Konrad Honold , Lothar Kling , Kurt Lang , Ulrike Leser
发明人： Konrad Honold , Lothar Kling , Kurt Lang , Ulrike Leser
IPC分类号： A61K47/48 , A61K38/16 , A61K38/46 , A61K38/49 , A61L27/00 , A61P43/00 , A61K38/00
CPC分类号： A61K38/49 , A61K38/164
摘要： Described herein is a method of promoting osteogenesis in a patient in need thereof by administering a plasminogen activator which activates the zymogen plasminogen by cleavage between arginine 506 and valine 561 to form the serine protease plasmin.
摘要翻译： PCT No.PCT / EP96 / 03345 Sec。 371日期：1998年2月9日 102（e）1998年2月9日PCT PCT 1996年7月30日PCT公布。公开号WO97 / 05891 日期1997年2月20日描述了通过施用纤维蛋白溶酶原激活剂来促进有需要的患者的成骨的方法，所述纤溶酶原激活剂通过在精氨酸506和缬氨酸561之间切割而激活酶原纤溶酶原，以形成丝氨酸蛋白酶纤溶酶。

5. 发明授权

US07052514B2 Methods and reagents for tissue engineering of cartilage in vitro 失效
公开(公告)号：US07052514B2
公开(公告)日：2006-05-30
申请号：US09844257
申请日：2001-04-27
申请人： Karin Kellner , Kurt Lang , Apollon Papadimitriou , Ulrike Leser-Reiff , Michaela Schultz , Torsten Blunk , Achim Goepferich
发明人： Karin Kellner , Kurt Lang , Apollon Papadimitriou , Ulrike Leser-Reiff , Michaela Schultz , Torsten Blunk , Achim Goepferich
IPC分类号： A61F3/00 , A61F5/00 , G01N33/567
CPC分类号： A61L27/3852 , A61L27/3612 , A61L27/3654 , A61L27/3843 , A61L2430/06
摘要： The present invention makes available an optimal concentration of a hedgehog polypeptide for modulating growth and/or cartilage production by chondrocytes. The present invention allows for improvements in the culturing of chondrocytes to develop cartilaginous tissue ex vivo suitable for implantation to replace damaged or deteriorated cartilage in a patient.

6. 发明申请

US20100035817A1 METHOD FOR THE PRODUCTION OF CONJUGATES OF INSULIN-LIKE GROWTH FACTOR-1 AND POLY(ETHYLENE GLYCOL) 审中-公开
标题翻译：产生胰岛素样生长因子-1和聚（乙二醇）的方法
公开(公告)号：US20100035817A1
公开(公告)日：2010-02-11
申请号：US12576266
申请日：2009-10-09
申请人： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
发明人： Stephan Fischer , Friederike Hesse , Hendrik Knoetgen , Kurt Lang , Friedrich Metzger , Joerg Thomas Regula , Christian Schantz , Andreas Schaubmar , Hans-Joachim Schoenfeld
IPC分类号： A61K38/18 , A61P25/28
CPC分类号： C07K14/65 , A61K47/60 , C07K2319/21 , C07K2319/50
摘要： The present invention relates to a fusion protein comprising IGF-I or an IGF-I variant N-terminally linked to the C-terminus of a propeptide. The invention relates also to a method involving the use of the aforementioned fusion protein in the production of a lysine-PEGylated IGF-I or IGF-I variant. The method comprises the steps of cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding the fusion protein and causing the cell to express the fusion protein, recovering and PEGylating said fusion protein, cleaving said PEGylated fusion protein with IgA protease, and recovering lysine-PEGylated IGF-I or IGF-I variant. The invention relates also to a lysine-PEGylated IGF-I or IGF-I variant produced using the above method. In addition, the invention relates to a method for treating a neurodegenerative disorders like Alzheimer's Disease using the lysine-PEGylated IGF-I or IGF-I variant and a composition comprising the lysine-PEGylated IGF-I or IGF-I variant.
摘要翻译：本发明涉及包含IGF-I或与前肽的C-末端N末端连接的IGF-1变体的融合蛋白。本发明还涉及涉及在制备赖氨酸 - PEG化IGF-I或IGF-I变体中使用上述融合蛋白的方法。该方法包括以下步骤：培养包含含有编码融合蛋白的核酸的表达载体的原核宿主细胞，并使细胞表达融合蛋白，回收聚乙二醇化所述融合蛋白，用IgA蛋白酶切割所述聚乙二醇化融合蛋白，以及回收赖氨酸 - PEG化IGF-I或IGF-I变体。本发明还涉及使用上述方法制备的赖氨酸 - 聚乙二醇化IGF-I或IGF-I变体。此外，本发明涉及使用赖氨酸PEG化IGF-I或IGF-1变体和包含赖氨酸 - PEG化IGF-I或IGF-I变体的组合物治疗神经变性疾病如阿尔茨海默病的方法。

7. 发明申请

US20050033035A1 Mutants of igf binding proteins and methods of production of antagonists thereof 审中-公开
标题翻译： igf结合蛋白的突变体及其拮抗剂的生产方法
公开(公告)号：US20050033035A1
公开(公告)日：2005-02-10
申请号：US10479819
申请日：2002-06-05
申请人： Hans-Georg Beisel , Dirk Demuth , Richard Engh , Tadeusz Holak , Robert Huber , Kurt Lang , Ralf Schumacher , Wojciech Zeslawski
发明人： Hans-Georg Beisel , Dirk Demuth , Richard Engh , Tadeusz Holak , Robert Huber , Kurt Lang , Ralf Schumacher , Wojciech Zeslawski
IPC分类号： C12N15/09 , A61K38/00 , C07K1/30 , C07K14/47 , C07K14/65 , C07K19/00 , G06F19/00 , G01N33/48 , G01N33/50
CPC分类号： C07K14/65 , A61K38/00 , C07K14/4743 , C07K2299/00
摘要： The present invention provides a crystal suitable for X-ray diffraction, comprising a complex of insulin-like growth factor I or II (IGF) and a polypeptide consisting of the amino acids 39-91 of IGFBP-1, the amino acids 55-107 of IGFBP-2, the amino acids 47-99 of IGFBP-3, the amino acids 39-91 of IGFBP-4, the amino acids 40-92 of IGFBP-5, or the amino acids 40-92 of IGFBP-6 or a fragment thereof consisting at least of the 9th to 12th cysteine of IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, or IGFBP-5 or at least of the 7th to 10th cysteine of IGFBP-6; methods for the determination of the atomic coordinates of such a crystal; IGFBP mutants with enhanced binding affinity for IGF-I and/or IGF-II, and methods to identify and optimize small molecules which displace IGFs from their binding proteins.
摘要翻译：本发明提供了适用于X射线衍射的晶体，其包含胰岛素样生长因子I或II（IGF）的复合物和由IGFBP-1的氨基酸39-91，氨基酸55-107 的IGFBP-2，IGFBP-3的氨基酸47-99，IGFBP-4的氨基酸39-91，IGFBP-5的氨基酸40-92或IGFBP-6的氨基酸40-92或其IGFBP-1，IGFBP-2，IGFBP-3，IGFBP-4或IGFBP-5的至少9个至12个半胱氨酸或至少7个至10个的片段 IGFBP-6的半胱氨酸; 用于确定这种晶体的原子坐标的方法; 对IGF-I和/或IGF-II具有增强的结合亲和力的IGFBP突变体以及鉴定和优化从其结合蛋白取代IGF的小分子的方法。

8. 发明授权

US06468978B1 Active hedgehog protein conjugate 失效
标题翻译：活性刺猬蛋白结合物
公开(公告)号：US06468978B1
公开(公告)日：2002-10-22
申请号：US09301199
申请日：1999-04-28
申请人： Angelika Esswein , Kurt Lang , Petra Rueger , Tilman Seytter
发明人： Angelika Esswein , Kurt Lang , Petra Rueger , Tilman Seytter
IPC分类号： A61K3817
CPC分类号： A61K31/185 , A61K31/727 , A61K38/00 , C07K14/47
摘要： A hedgehog conjugate which is characterized in that it contains: a) a polypeptide composed of 10 to 30 hydrophobic amino acids and/or amino acids which form transmembrane helices and are positively charged, b) 1 to 4 aliphatic, saturated or unsaturated hydrocarbon residues with a chain length of 10 to 24 C atoms and with a hydrophobic action or c) a hydrophobic thio compound covalently bound to a hedgehog protein and which has a several-fold increased activity and is suitable as a pharmaceutical agent.
摘要翻译：一种刺猬蛋白缀合物，其特征在于其包含：a）由10至30个疏水氨基酸和/或氨基酸组成的多肽，其形成跨膜螺旋并带正电荷，b）1至4个脂族，饱和或不饱和的烃残基，链长为10至24个C原子并具有疏水作用，或c）与猬蛋白共价结合的疏水性硫代化合物，并且其具有几倍增加的活性并且适于作为药剂。

9. 发明授权

US5749216A Open end spinning apparatus 失效
标题翻译：开口纺纱设备
公开(公告)号：US5749216A
公开(公告)日：1998-05-12
申请号：US818307
申请日：1997-03-14
申请人： Kurt Lang , Friedbert Schmid
发明人： Kurt Lang , Friedbert Schmid
IPC分类号： D01H4/08 , D01H4/00
CPC分类号： D01H4/08
摘要： In the case of an open-end spinning arrangement, a spinning rotor is arranged in a rotor housing, to which a vacuum of at least 500 mm water column is applied. A fibre feed channel, beginning at an opening roller with an entry opening, is directed with an outlet opening at a sliding wall of the spinning rotor. The fibre feed channel bends slightly in the direction towards a fibre collecting groove of the spinning rotor. The cross sectional area of the outlet opening of the fibre feed channel measures at least 19 mm.sup.2 and amounts to at least 30% of the cross sectional area of the entry opening. As a result of the bend in the fibre feed channel, the fibres are collected together in a fibre bundle on a longitudinal side of the fibre feed channel. The fibre bundle occupies only a part area of the outlet opening when leaving the fibre feed channel and is fed with high precision onto the sliding wall. The outlet opening can thus have larger dimensions without this leading to inaccurate feeding of the fibres. The outlet opening possesses dimensions that permit a large volume of air to flow at high speed from the opening roller through the fibre feed channel. Fly accumulation in the area of the opening roller is thus avoided.
摘要翻译：在开放式纺纱装置的情况下，在转子壳体内设置有旋转转子，在该壳体内施加至少500mm的水柱的真空。从具有进入开口的打开辊开始的纤维进料通道与纺丝转子的滑动壁处的出口开口导向。纤维进料通道沿着纺丝转子的纤维收集槽的方向稍微弯曲。纤维进料通道的出口开口的横截面积至少为19mm 2，并且相当于入口的横截面面积的至少30％。由于纤维供给通道的弯曲，纤维在纤维供给通道的纵向侧以纤维束收集在一起。当离开纤维进料通道时，纤维束仅占出口开口的一部分区域，并且高精度地供给到滑动壁上。因此，出口开口可以具有更大的尺寸而不导致纤维的不准确的供给。出口开口具有允许大量空气以高速从打开辊通过纤维进料通道流动的尺寸。因此避免了在打开辊的区域中的飞累积。

10. 发明授权

US5304310A Method for concentrating and purifying hirudin from leeches 失效
标题翻译：从水蛭中浓缩和净化水蛭素的方法
公开(公告)号：US5304310A
公开(公告)日：1994-04-19
申请号：US910320
申请日：1992-07-19
申请人： Kurt Lang , Andreas Kreimeyer
发明人： Kurt Lang , Andreas Kreimeyer
IPC分类号： C12N15/09 , C07D475/04 , C07K1/30 , C07K14/815 , C12N9/00 , C12N9/76 , C12N15/15 , C12P21/00 , C12P21/02 , C12R1/19 , B01D11/02 , A61K35/62
CPC分类号： C12N9/6427 , C07D475/04 , C07K1/30 , C07K14/815 , C12N9/00 , Y10S530/855
摘要： A method for the concentration and purification of biomolecules is described and comprises introducing the biomolecule into an aqueous/organic solvent system and bringing about a phase separation by addition of salts, where the pH at which the concentration takes place, the solvents suitable for the aqueous/organic solvent system, and the nature of the salt are determined in a series of tests one embodiment concerns concentration of hirudin by using propanol, butanol or acetone a the solvent and sodium chloride as the salt.
摘要翻译： PCT No.PCT / EP91 / 02269 Sec。 371日期：1992年7月17日 102（e）日期1992年7月17日PCT 1990年12月20日PCT PCT。出版物WO91 / 10677 日期：1991年7月25日。描述了生物分子的浓缩和纯化方法，包括将生物分子引入水/有机溶剂体系中，并通过加入盐进行相分离，其中发生浓度的pH 适用于水/有机溶剂系统的溶剂和盐的性质在一系列试验中确定，一个实施方案涉及通过使用丙醇，丁醇或丙酮，溶剂和氯化钠作为盐的水蛭素的浓度。

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