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1. 发明申请

US20150355171A1 INDOXYL SULFATE MEASUREMENT METHOD 审中-公开
标题翻译：吲哚硫酸盐测定方法
公开(公告)号：US20150355171A1
公开(公告)日：2015-12-10
申请号：US14654656
申请日：2014-02-17
申请人： KUREHA CORPORATION
发明人： Hirotaka Hoshi , Kaori Kikuchi , Yoshiharu Itoh , Ayano Konagai
IPC分类号： G01N33/53 , C07K16/44
CPC分类号： G01N33/5308 , C07K16/44 , G01N2800/347
摘要： An object of the present invention is to provide a competitive EIA method capable of quantitatively measuring indoxyl sulfate in blood. The problem described above can be solved by an indoxyl sulfate measurement method based on a competitive method comprising: (1) a step in which albumin in a specimen is pretreated; (2) a specimen-antibody reaction step in which the pretreated specimen and anti-indoxyl sulfate antibodies are brought into contact with each other in the substantial absence of exogenous mammalian albumin; (3) an immobilized IS-antibody reaction step in which the specimen-antibody reaction solution is brought into contact, in the substantial absence of exogenous mammalian albumin, with an immobilized insoluble support in which indoxyl sulfate is immobilized and then blocked with a buffer solution containing substantially no mammalian albumin; and (4) a step in which anti-indoxyl sulfate antibodies which have bonded to the immobilized insoluble support are detected.
摘要翻译：本发明的目的是提供一种能够定量测定血液中硫酸吲哚酯的EIA方法。上述问题可以通过基于竞争方法的硫酸吲哚硫酸盐测定方法来解决，该方法包括：（1）预处理样品中的白蛋白的步骤; （2）在基本上不存在外源性哺乳动物白蛋白的情况下使预处理标本和抗吲哚硫酸盐抗体相互接触的标本 - 抗体反应步骤; （3）固定化的IS抗体反应步骤，其中在基本上不存在外源性哺乳动物白蛋白的情况下使样品抗体反应溶液与固定化的不溶性载体接触，其中固定有吲哚硫酸盐硫酸盐，然后用缓冲溶液基本上不含哺乳动物白蛋白; 和（4）检测与固定的不溶性载体结合的抗吲哚硫酸硫酸抗体的步骤。

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