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1. 发明授权

US09198365B2 Method to develop high oleic acid soybeans using conventional soybean breeding techniques 有权
公开(公告)号：US09198365B2
公开(公告)日：2015-12-01
申请号：US13351757
申请日：2012-01-17
申请人： Kristin D. Bilyeu , James Grover Shannon , Jeong-Dong Lee , Anh Tung Pham
发明人： Kristin D. Bilyeu , James Grover Shannon , Jeong-Dong Lee , Anh Tung Pham
IPC分类号： A01H5/10
CPC分类号： C12N15/8247 , A01H1/00 , A01H5/10
摘要： The present invention is directed to a soybean plant with mutations in FAD2-1A and FAD2-1B. Moreover, the present invention is directed to seeds from said plants with altered ratios of monosaturated and polyunsaturated fats. In particular, the present invention is directed to plants where the plants exhibit elevated levels of oleic acid.

2. 发明申请

US20120192306A1 Method To Develop High Oleic Acid Soybeans Using Conventional Soybean Breeding Techniques 有权
标题翻译：使用常规大豆育种技术开发高油酸大豆的方法
公开(公告)号：US20120192306A1
公开(公告)日：2012-07-26
申请号：US13351757
申请日：2012-01-17
申请人： Kristin D. Bilyeu , James Grover Shannon , Jeong-Dong Lee , Anh Tung Pham
发明人： Kristin D. Bilyeu , James Grover Shannon , Jeong-Dong Lee , Anh Tung Pham
IPC分类号： A01H1/02 , A01H5/00
CPC分类号： C12N15/8247 , A01H1/00 , A01H5/10
摘要： The present invention is directed to a soybean plant with mutations in FAD2-1A and FAD2-1B. Moreover, the present invention is directed to seeds from said plants with altered ratios of monosaturated and polyunsaturated fats. In particular, the present invention is directed to plants where the plants exhibit elevated levels of oleic acid.
摘要翻译：本发明涉及具有FAD2-1A和FAD2-1B突变的大豆植物。此外，本发明涉及具有改变的单饱和脂肪和多不饱和脂肪比例的来自所述植物的种子。特别地，本发明涉及植物表现出较高水平的油酸的植物。

3. 发明申请

US20100190154A1 Development of low allergen soybean seeds using molecular markers for the P34 allele 有权
标题翻译：使用P34等位基因的分子标记开发低变应原大豆种子
公开(公告)号：US20100190154A1
公开(公告)日：2010-07-29
申请号：US12322033
申请日：2009-01-28
申请人： Kristin D. Bilyeu
发明人： Kristin D. Bilyeu
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C12Q2600/156
摘要： A mutation in the gene encoding the P34 protein in soybean which affects allergenicity is characterized. Soybean homozygous for a mutant allele comprising a four base pair insertion at the start codon of the gene encoding the P34 protein, exhibit significantly reduced P34 protein accumulation. Nucleic acid samples of soybean may be assayed for the presence of this insertion to detect the mutant allele, and soybean containing the allele may be selected for breeding to generate reduced P34 soybean lines. Molecular markers have been developed for detecting the presence or absence of the four base pair insertion.
摘要翻译：表征大豆中编码P34蛋白的基因突变，其影响变应原性。在编码P34蛋白的基因的起始密码子上包含四碱基对插入的突变等位基因纯合的大豆显示出显着降低的P34蛋白质积累。可以测定大豆的核酸样品是否存在该插入物以检测突变体等位基因，并且可以选择含有等位基因的大豆进行育种以产生减少的P34大豆品系。已经开发了用于检测四碱基对插入的存在或不存在的分子标记。

4. 发明授权

US08728726B1 RS2 mutant allele, perfect molecular markers, and low raffinose/stachyose soybean germplasm 有权
标题翻译： RS2突变体等位基因，完美分子标记和低棉子糖/水苏糖大豆种质
公开(公告)号：US08728726B1
公开(公告)日：2014-05-20
申请号：US13176910
申请日：2011-07-06
申请人： Kristin D. Bilyeu , Emily C. Dierking
发明人： Kristin D. Bilyeu , Emily C. Dierking
IPC分类号： A01H5/00 , C12Q1/68 , A01H1/04
CPC分类号： C12Q1/6895 , A01H5/10 , C12N9/1051 , C12Q2600/156
摘要： A mutation in the gene encoding the raffinose synthase 2 enzyme, RS2, in soybean, Glycine max (L.) Merr., is associated with a reduced raffinose and stachyose seed phenotype. Soybean homozygous for a mutant allele of the RS2 gene which encodes an amino acid change to isoleucine at position 107 of the enzyme's amino acid sequence exhibit significantly reduced seed raffinose and stachyose content, and increased seed sucrose content. Nucleic acid samples of soybean may be assayed for the presence of this mutant allele, and soybean containing the allele may be selected for breeding to generate reduced raffinose and stachyose soybean lines. Alternatively, the mutation may be detected by analysis of the RS2 enzyme produced in the soybean to determine the presence of either an isoleucine or a threonine at amino acid 107. Molecular markers have been developed for detecting the presence or absence of the mutant allele.
摘要翻译：编码棉子糖合成酶2的酶基因RS2在大豆（Glycine max（L.）Merr。）中的突变与降解的棉子糖和水苏糖种子表型有关。对于编码氨基酸序列的位置107处的异亮氨酸的氨基酸变化的RS2基因的突变等位基因纯合的大豆显示出显着降低的种子棉子糖和水苏糖含量，以及增加的种子蔗糖含量。可以测定大豆的核酸样品中该突变体等位基因的存在，并且可以选择含有等位基因的大豆进行育种以产生还原的棉子糖和水苏糖大豆品系。或者，可以通过分析在大豆中产生的RS2酶来检测突变，以确定氨基酸107存在异亮氨酸或苏氨酸。已经开发了用于检测突变等位基因存在或不存在的分子标记。

5. 发明授权

US08471107B2 Soybeans having high germination rates and ultra-low raffinose and stachyose content 有权
标题翻译：大豆具有高发芽率和超低棉子糖和水苏糖含量
公开(公告)号：US08471107B2
公开(公告)日：2013-06-25
申请号：US12762097
申请日：2010-04-16
申请人： John A. Schillinger , Emily C. Dierking , Kristin D. Bilyeu
发明人： John A. Schillinger , Emily C. Dierking , Kristin D. Bilyeu
IPC分类号： A01H5/00 , A01H5/10 , C12N15/29 , C12N15/82
CPC分类号： C12N15/8245 , A01H5/10 , C12N9/1051
摘要： Soybean seeds having an ultra-low raffinose and stachyose phenotype. Also disclosed is a mutant allele of soybean designated SG-ULRFO which results in an ultra-low raffinose and stachyose phenotype. The present invention also relates to a soybean seed, a soybean plant and parts of a soybean plant and a soybean hybrid which comprises the mutant allele. Also disclosed are ultra-low raffinose and stachyose soybean seeds having unexpectedly increased germination rates when compared with soybean lines not having a low raffinose and stachyose seed content. Also disclosed are mutant RS3 and RS4 genes with polymorphisms which contribute to the ultra-low raffinose and stachyose phenotype as described in the present invention. The present invention also relates for method of using the soybean seeds and plants of the present invention, to plants parts derived from the present invention, to methods of producing transgenic plants using the plants and seeds of the present invention.
摘要翻译：具有超低棉子糖和水苏糖表型的大豆种子。还公开了指定为SG-ULRFO的大豆的突变体等位基因，其导致超低棉子糖和水苏糖表型。本发明还涉及大豆种子，大豆植物和大豆植物的部分以及包含突变等位基因的大豆杂种。还公开了与不具有低棉子糖和水苏糖种子含量的大豆品系相比，超低棉子糖和水苏糖大豆种子具有出乎意料地增加的发芽率。还公开了具有如本发明所述的对超低棉子糖和水苏糖表型有贡献的多态性的突变体RS3和RS4基因。本发明还涉及将本发明的大豆种子和植物，本发明的植物成分用于使用本发明的植物和种子生产转基因植物的方法。

6. 发明授权

US07951537B2 Development of low allergen soybean seeds using molecular markers for the P34 allele 有权
标题翻译：使用P34等位基因的分子标记开发低变应原大豆种子
公开(公告)号：US07951537B2
公开(公告)日：2011-05-31
申请号：US12322033
申请日：2009-01-28
申请人： Kristin D. Bilyeu
发明人： Kristin D. Bilyeu
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02
CPC分类号： C12Q1/6895 , C12Q2600/156
摘要： A mutation in the gene encoding the P34 protein in soybean which affects allergenicity is characterized. Soybean homozygous for a mutant allele comprising a four base pair insertion at the start codon of the gene encoding the P34 protein, exhibit significantly reduced P34 protein accumulation. Nucleic acid samples of soybean may be assayed for the presence of this insertion to detect the mutant allele, and soybean containing the allele may be selected for breeding to generate reduced P34 soybean lines. Molecular markers have been developed for detecting the presence or absence of the four base pair insertion.
摘要翻译：表征大豆中编码P34蛋白的基因突变，其影响变应原性。在编码P34蛋白的基因的起始密码子上包含四碱基对插入的突变等位基因纯合的大豆显示出显着降低的P34蛋白质积累。可以测定大豆的核酸样品是否存在该插入物以检测突变体等位基因，并且可以选择含有等位基因的大豆进行育种以产生减少的P34大豆品系。已经开发了用于检测四碱基对插入的存在或不存在的分子标记。

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