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1. 发明申请

US20070166708A1 Methods for detection and quantification of analytes in complex mixtures 有权
标题翻译：复杂混合物中分析物的检测和定量方法
公开(公告)号：US20070166708A1
公开(公告)日：2007-07-19
申请号：US10542458
申请日：2002-07-03
申请人： Krassen Dimitrov , Dwayne Dunaway
发明人： Krassen Dimitrov , Dwayne Dunaway
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6888 , C12Q1/6816 , C12Q1/682 , C12Q1/6876 , C12Q2565/102 , C12Q2537/143 , C12Q2525/161 , C12Q2565/1025 , C12Q2563/179 , C12Q2525/151 , C12Q2525/101
摘要： The invention provides a diverse population of uniquely labeled probes, containing about thirty or more target specific nucleic acid probes each attached to a unique label bound to a nucleic acid. Also provided is a method of producing a population of uniquely labeled nucleic acid probes. The method consists of (a) synthesizing a population of target specific nucleic acid probes each having a different specifier; (b) synthesizing a corresponding population of anti-genedigits each having a unique label, the population having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) hybridizing the populations of target nucleic acid probes to the anti-genedigits, to produce a population in which each of the target specific probes is uniquely labeled. Also provided is a method of detecting a nucleic acid analyte. The method consists of (a) contacting a mixture of nucleic acid analytes under conditions sufficient for hybridization with a plurality of target specific nucleic acid probes each having a different specifier; (b) contacting the mixture under conditions sufficient for hybridization with a corresponding plurality of anti-genedigits each having a unique label, the plurality of anti-genedigits having a diversity sufficient to uniquely hybridize to genedigits within the specifiers, and (c) uniquely detecting a hybridized complex between one or more analytes in the mixture, a target specific probe, and an anti-genedigit.
摘要翻译：本发明提供了多种独特标记的探针，其含有约30个或更多个目标特异性核酸探针，其各自附着于与核酸结合的唯一标记。还提供了生产独特标记的核酸探针群体的方法。该方法包括（a）合成具有不同说明符的目标特异性核酸探针群; （b）合成各自具有独特标记的相应群体的抗Genieigits，所述群体具有足以独特地与所述说明者内的Genesigits杂交的多样性，以及（c）将靶核酸探针的群体与抗Genieigits杂交，以产生其中每个靶特异性探针被唯一标记的群体。还提供了检测核酸分析物的方法。该方法包括（a）在足以与多个具有不同说明符的靶特异性核酸探针杂交的条件下使核酸分析物的混合物接触; （b）在足以与各自具有唯一标记的相应多个抗基因组杂交的条件下接触混合物，所述多个抗Genetigits具有足以独特地与指定剂内的Genesigits杂交的分集，以及（c）唯一检测混合物中一种或多种分析物之间的杂交复合物，靶标特异性探针和抗Genetigigit。

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