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1. 发明申请

US20070033676A1 Process for producing plant storage organ with high production of recombinant protein and novel recombinant protein 失效
标题翻译：生产重组蛋白和新型重组蛋白的植物贮藏器具生产工艺
公开(公告)号：US20070033676A1
公开(公告)日：2007-02-08
申请号：US10550624
申请日：2004-03-26
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Humio Takaiwa , Takahito Jomori , Yuji Hayashi , Akira Tashita , Yukari Kobara
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Humio Takaiwa , Takahito Jomori , Yuji Hayashi , Akira Tashita , Yukari Kobara
IPC分类号： A01H5/00 , C12N15/82 , A01H1/00 , C07K14/605
CPC分类号： C12N15/8257 , C07K14/605 , C12N15/8216
摘要： The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.
摘要翻译：本发明提供了在植物贮藏器官和GLP-1衍生物中高度产生重组蛋白的方法。通过使用包含重组蛋白基因，细胞分裂素相关基因，耐药性基因和可移除DNA元件的载体转化获得重组蛋白质高度产生的植物贮藏器官，其中细胞分裂素相关基因和耐药基因存在于与DNA元件一起表现的位置，而在植物储存器官中表达的重组蛋白存在于与DNA不一致的位置元件。通过使用该方法制备GLP-1，进一步提供了稳定的酶促消化的衍生物。

2. 发明授权

US07947876B2 Plant and plant storage organ having GLP-1 derivative accumulated therein and method of producing the same 失效
标题翻译：蓄积有GLP-1衍生物的植物贮藏器具及其制造方法
公开(公告)号：US07947876B2
公开(公告)日：2011-05-24
申请号：US11662650
申请日：2004-09-14
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Humio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Humio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： A61K38/26 , A23L33/105 , C12N15/8257
摘要： The present invention is regarding plants and plant storage organs thereof in which GLP-1 derivatives are accumulated, and methods of producing them. The transgenic plants and plant storage organs thereof accumulate tandem repeated GLP-1 derivatives cleavable with intestinal digestive enzyme to monomeric molecules and are produced by methods comprising: integrating into vectors linked DNAs which comprise tandem repeated DNAs encoding the GLP-1 derivative with trypsin resistance in which the amino acid in the 26th position is Gln, the amino acid in the 34th position is Asn or Asp, and C-terminal consists of Arg or Lys to produce monomeric molecules; introducing the vectors into plant cells; and redifferentiating the obtained transformants. The edible transgenic plants and plant storage organs are useful for treating diabetes and can be ingested by diabetic patients.
摘要翻译：本发明涉及其中积累GLP-1衍生物的植物和植物贮藏器官及其生产方法。其转基因植物和植物储存器官将可与肠消化酶切割的串联重复的GLP-1衍生物积聚到单体分子中，并通过以下方法产生：包括：将包含编码GLP-1衍生物的串联重复DNA与胰蛋白酶抗性的连锁DNA连接其中第26位的氨基酸是Gln，第34位的氨基酸是Asn或Asp，C末端由Arg或Lys组成以产生单体分子; 将载体导入植物细胞; 并重新分化获得的转化体。可食用的转基因植物和植物贮藏器官可用于治疗糖尿病并可被糖尿病患者摄入。

3. 发明授权

US07847063B2 GLP-1 derivative 失效
标题翻译： GLP-1衍生物
公开(公告)号：US07847063B2
公开(公告)日：2010-12-07
申请号：US10550624
申请日：2004-03-26
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Takahito Jomori , Yuji Hayashi , Akira Tashita , Yukari Kobara
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Takahito Jomori , Yuji Hayashi , Akira Tashita , Yukari Kobara
IPC分类号： A61K38/00 , C07K14/00
CPC分类号： C12N15/8257 , C07K14/605 , C12N15/8216
摘要： The present invention provides a method for highly producing a recombinant protein in a plant storage organ and a GLP-1 derivative. The plant storage organ in which the recombinant protein is highly produced is obtained by transformation with the use of a vector which comprises a recombinant protein gene, a cytokinin-related gene, a drug-resistant gene and a removable DNA element, in which the cytokinin-related gene and the drug-resistant gene exist in the positions so that they can behave together with the DNA element, while the recombinant protein to be expressed in the plant storage organ exists in the position so that it would not behave together with the DNA element. The GLP-1 is produced by using the method, and a derivative having been stabilized against enzymatic digestion is further provided.
摘要翻译：本发明提供了在植物贮藏器官和GLP-1衍生物中高度产生重组蛋白的方法。通过使用包含重组蛋白基因，细胞分裂素相关基因，耐药性基因和可移除DNA元件的载体转化获得重组蛋白质高度产生的植物贮藏器官，其中细胞分裂素相关基因和耐药基因存在于可以与DNA元件一起表现的位置，而在植物储存器官中表达的重组蛋白存在于与DNA不一致的位置元件。通过使用该方法制备GLP-1，进一步提供了稳定的酶促消化的衍生物。

4. 发明申请

US20080292732A1 Plant and Plant Storage Organ Having Glp-1 Derivative Accumulated Therein and Method of Producing the Same 失效
标题翻译：含有Glp-1衍生物的植物和植物储存器官及其生产方法
公开(公告)号：US20080292732A1
公开(公告)日：2008-11-27
申请号：US11662650
申请日：2004-09-14
申请人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
发明人： Koichi Sugita , Saori Kasahara , Hiroyasu Ebinuma , Fumio Takaiwa , Hiroshi Yasuda , Takahito Jomori , Yuji Hayashi , Akira Tashita
IPC分类号： A61K36/00 , C12N15/82 , A01H5/00 , A61P31/10
CPC分类号： A61K38/26 , A23L33/105 , C12N15/8257
摘要： The present invention is to provide a plant and a plant storage organ thereof in which GLP-1 derivatives are accumulated, and a method of producing them in order to develop a method for ingesting orally GLP-1 derivatives at a low cost and to make use of it in diabetic treatment. A transgenic plant or a plant storage organ thereof in which GLP-1 derivatives are accumulated cleavable with a digestive enzyme is produced by a method comprising: integrating into a vector a linked GLP-1s-DNA which comprises tandem repeated “n” DNAs (“n” being an integer of 3 or more) encoding a GLP-1 derivative consisting of GLP-1 (7-36) or of an amino acid sequence of GLP-1 (7-36) in which one or a few amino acids are deleted, substituted and/or added, and C-terminal consists of Arg or Lys, and having GLP-1 activity; introducing the vector into a plant cell; and redifferentiating the obtained transformant. The transgenic plant and the plant storage organ are useful as a pharmaceutical composition or a food or drink for treating or preventing diabetes.
摘要翻译：本发明提供一种其中积聚有GLP-1衍生物的植物和植物贮藏器官及其生产方法，以便开发低成本摄取口服GLP-1衍生物的方法并使用的糖尿病治疗。 GLP-1衍生物可以用消化酶积聚可切割的转基因植物或植物贮藏器官是通过以下方法产生的：将连接的GLP-1s-DNA整合到载体中，所述连接的GLP-1s-DNA包含串联重复的“n”DNA（“ 编号由GLP-1（7-36）或GLP-1（7-36）的氨基酸序列组成的GLP-1衍生物的n“为3以上的整数，其中一个或几个氨基酸为缺失，取代和/或添加，C-末端由Arg或Lys组成，具有GLP-1活性; 将载体导入植物细胞; 并对所得转化体进行重新分化。转基因植物和植物贮藏器官可用作药物组合物或用于治疗或预防糖尿病的食物或饮料。

5. 发明授权

US07291594B2 GLP-1 derivative and preparation thereof absorbable via mucous membrane 失效
标题翻译： GLP-1衍生物及其可通过粘膜吸收的制剂
公开(公告)号：US07291594B2
公开(公告)日：2007-11-06
申请号：US10530125
申请日：2003-10-10
申请人： Yuji Hayashi , Mitsuhiro Makino , Toshiyuki Kouzaki , Motohiro Takeda , Takahito Jomori
发明人： Yuji Hayashi , Mitsuhiro Makino , Toshiyuki Kouzaki , Motohiro Takeda , Takahito Jomori
IPC分类号： A61K38/26 , C07K14/605
CPC分类号： C07K14/605 , A61K38/26 , Y10S514/866
摘要： A GLP-1 derivative is provided including an amino acid sequence of GLP-1 (7-35) having deletion, substitution and/or addition of one or more amino acids and having Waa-(Xaa)n-Yaa (in which Waa is Arg or Lys, Xaa is Arg or Lys, n is an integer of 0 to 14, and Yaa is Arg, Arg-NH2, Lys, Lys-NH2 or Hse) added to the C-terminus of the peptide having a GLP-1 activity. These derivatives are highly absorbable via a mucous membrane. The GLP-1 derivative can be conferred with resistance to dipeptidyl peptidase IV by substituting amino acid 8 in its GLP-1 amino acid sequence with Ser, or with resistance to trypsin by substituting amino acids 26 and 34 with Gln and Asn, respectively.The absorption efficiency of the GLP-1 derivatives via mucous membranes can be further improved by preparing a composition using a charge-regulated fat emulsion regulated to be negatively charged thereon.
摘要翻译：提供GLP-1衍生物，其包含具有缺失，取代和/或添加一个或多个氨基酸并具有Waa-（Xaa）n-Yaa的GLP-1（7-35）的氨基酸序列（其中Waa为 Arg或Lys，Xaa是Arg或Lys，n是0-14的整数，Yaa是Arg，Arg-NH 2，Lys，Lys-NH 2或 Hse）加入具有GLP-1活性的肽的C末端。这些衍生物通过粘膜是高度可吸收的。通过用Ser取代其GLP-1氨基酸序列中的氨基酸8，或分别用Gln和Asn取代氨基酸26和34，对胰蛋白酶具有抗性，GLP-1衍生物可赋予对二肽基肽酶IV的抗性。通过使用调节为带负电荷的电荷调节脂肪乳液制备组合物，可以进一步提高通过粘膜的GLP-1衍生物的吸收效率。

6. 发明申请

US20060194720A1 Glp-1 derivatives and transmicosal absorption preparations thereof 失效
标题翻译： Glp-1衍生物及其经半吸收制剂
公开(公告)号：US20060194720A1
公开(公告)日：2006-08-31
申请号：US10530125
申请日：2003-10-10
申请人： Yuji Hayashi , Mitsuhiro Makino , Toshiyuki Kouzaki , Motohiro Takeda , Takahito Jomori
发明人： Yuji Hayashi , Mitsuhiro Makino , Toshiyuki Kouzaki , Motohiro Takeda , Takahito Jomori
IPC分类号： C07K14/605 , A61K38/22
CPC分类号： C07K14/605 , A61K38/26 , Y10S514/866
摘要： The invention relates to a GLP-1 derivative including an amino acid sequence of GLP-1 (7-35) having deletion, substitution and/or addition of one or more amino acids and having Waa-(Xaa)n-Yaa (in which Waa is Arg or Lys, Xaa is Arg or Lys, n is an integer of 0 to 14, and Yaa is Arg, Arg-NH2, Lys, Lys-NH2 or Hse) added to the C-terminus of the peptide having a GLP-1 activity. These derivatives are derivatives highly absorbable via a mucous membrane. In the invention, the GLP-1 derivative can be conferred with resistance to dipeptidyl peptidase IV by substituting amino acid 8 in its GLP-1 amino acid sequence with Ser, or with resistance to trypsin by substituting amino acids 26 and 34 with Gln and Asn, respectively. The efficiency of absorption of the GLP-1 derivatives of the invention via mucous membranes can be further improved by preparing a composition using a charge-regulated fat emulsion regulated to be negatively charged thereon.
摘要翻译：本发明涉及包含具有缺失，取代和/或添加一个或多个氨基酸并具有Waa-（Xaa）n-Yaa）的GLP-1（7-35）氨基酸序列的GLP-1衍生物（其中 Waa为Arg或Lys，Xaa为Arg或Lys，n为0〜14的整数，Yaa为Arg，Arg-NH 2 Lys，Lys-NH 2 >或Hse）加入具有GLP-1活性的肽的C末端。这些衍生物是通过粘膜高度吸收的衍生物。在本发明中，通过用Ser取代其GLP-1氨基酸序列中的氨基酸8或通过用Gln和Asn取代氨基酸26和34对胰蛋白酶具有抗性，GLP-1衍生物可赋予对二肽基肽酶IV的抗性，分别。本发明的GLP-1衍生物通过粘膜吸收的效率可以通过使用调节为带负电荷的电荷调节脂肪乳剂制备组合物而进一步改善。

7. 发明授权

US08489266B2 Vehicle power system 有权
标题翻译：车载电源系统
公开(公告)号：US08489266B2
公开(公告)日：2013-07-16
申请号：US13050122
申请日：2011-03-17
申请人： Nobumasa Ueda , Naoki Yamamoto , Yuji Hayashi , Yuuki Tsutsu , Takuto Suzuki
发明人： Nobumasa Ueda , Naoki Yamamoto , Yuji Hayashi , Yuuki Tsutsu , Takuto Suzuki
IPC分类号： B60L9/00
CPC分类号： B60L3/04 , B60L1/003 , B60L3/0084 , B60L11/14 , Y02T10/70 , Y02T10/7077 , Y02T10/7258
摘要： A vehicle power system is provided with a switching power supply that generates an operation power for operating a plurality of ECU by stepping down a power supply supplied from at least one of a generator and a battery, and a power distributor that distributes and supplies the operation power generated by the switching power supply to the individual ECUs.
摘要翻译：车辆动力系统设置有开关电源，其通过降低从发电机和电池中的至少一个供给的电力产生用于操作多个ECU的操作电力，以及分配和供应操作的电力分配器由开关电源向各个ECU产生的电力。

8. 发明授权

US08420175B2 Composition containing crystalline cellulose composite 有权
标题翻译：含有结晶纤维素复合材料的组合物
公开(公告)号：US08420175B2
公开(公告)日：2013-04-16
申请号：US12995234
申请日：2009-06-08
申请人： Yuji Hayashi , Yukio Yamawaki
发明人： Yuji Hayashi , Yukio Yamawaki
IPC分类号： B05D3/02
CPC分类号： C09D11/16 , C08L1/04 , C08L1/286 , C08L3/02 , C08L5/00 , C08L2205/14 , C09D11/18 , C09D11/322 , C09D101/04 , C08L2666/26 , C08L2666/16
摘要： Disclosed is a composition containing 100 parts by mass of a pigment, 0.01-50 parts by mass of a cellulose composite and water. The composition has a solid content of more than 25% by mass, and a viscosity of not less than 30 mPa·S. The cellulose composite contains 0.5-50% by mass of a hydrophilic polymer having an acid value of not less than 2.0 and a weight average molecular weight of from 1×103 to 1×107 and 50-99.5% by mass of a crystalline cellulose. The cellulose composite has an average particle diameter of not more than 10 μm.
摘要翻译：公开了含有100质量份颜料，0.01-50质量份纤维素复合物和水的组合物。该组合物的固体成分为25质量％以上，粘度为30mPa·S以上。该纤维素复合体含有0.5〜50质量％的酸值为2.0以上的亲水性聚合物，结晶纤维素的重均分子量为1×10 3〜1×10 7，质量为50〜99.5质量％。纤维素复合体的平均粒径为10μm以下。

9. 发明授权

US08292017B2 Motorcycle 有权
标题翻译：摩托车
公开(公告)号：US08292017B2
公开(公告)日：2012-10-23
申请号：US13196453
申请日：2011-08-02
申请人： Yusuke Inoue , Satoshi Matsushima , Tatsuya Sasaki , Hiroshi Nakashima , Yuji Hayashi
发明人： Yusuke Inoue , Satoshi Matsushima , Tatsuya Sasaki , Hiroshi Nakashima , Yuji Hayashi
IPC分类号： B62D61/02 , B62K11/00
CPC分类号： F01N1/24 , F01N1/10 , F01N13/1811
摘要： A silencer for a motorcycle has a front cup which has a tapered tubular shape, a center sleeve which is connected to the front cup, and a tail cup which is connected to the center sleeve. Since the front cup has a tapered tubular shape, the front cup is arranged such that a front end of the front cup is positioned in the vicinity of a main frame. A distal end of the silencer can be shifted frontward in the longitudinal direction of a vehicle compared to a silencer of a prior art. As a result, a length of the silencer can be increased while avoiding interference between the silencer and legs of a rider and hence, silencing performance of the silencer can be enhanced. Since the distal end of the silencer can be shifted frontward in the longitudinal direction of the vehicle, the concentration of a mass can be acquired.
摘要翻译：用于摩托车的消声器具有锥形管状的前杯，连接到前杯的中心套和连接到中心套的尾杯。由于前杯具有锥形管状，所以前杯配置成使前杯的前端位于主框架附近。与现有技术的消声器相比，消声器的远端可以在车辆的纵向方向上向前移动。结果，消音器的长度可以增加，同时避免消音器与骑手的消音器之间的干扰，从而可以提高消音器的消声性能。由于消音器的远端可以沿着车辆的纵向方向向前移动，所以可以获得质量的集中。

10. 发明授权

US07773399B2 Control apparatus for inhibiting synchronous-rectifier switching elements at low output current in a voltage transforming apparatus 有权
标题翻译：用于在电压变换装置中以低输出电流抑制同步整流器开关元件的控制装置
公开(公告)号：US07773399B2
公开(公告)日：2010-08-10
申请号：US11699075
申请日：2007-01-29
申请人： Kimikazu Nakamura , Tsuyoshi Yamashita , Yuji Hayashi
发明人： Kimikazu Nakamura , Tsuyoshi Yamashita , Yuji Hayashi
IPC分类号： H02M3/335
CPC分类号： H02M3/33592 , Y02B70/1475
摘要： The control apparatus for controlling a voltage transforming apparatus having a transformer, power switching elements disposed in a primary side, and synchronous-rectifying switching elements disposed in a secondary side includes a judging circuit making a judgment as to whether or not an output current of the voltage transforming apparatus is smaller than a specified current on the basis of a primary-side current of the transformer and an inhibition circuit inhibiting the synchronous-rectifying switching elements from performing their synchronous-rectifying control operation when the judging circuit judges that the output current is smaller than the specified current. The judging circuit makes the judgment on the basis of the primary-side current flowing through the primary coil of the transformer immediately before the power switching elements are turned off.
摘要翻译：用于控制具有变压器的电压变换装置的控制装置，设置在初级侧的功率开关元件和设置在次级侧的同步整流开关元件包括判断电路，判断是否输出电流电压变换装置基于变压器的初级侧电流小于规定电流，抑制电路在判定电路判断为输出电流为止时，禁止同步整流用开关元件进行同步整流控制动作小于指定电流。判断电路基于在功率开关元件断开之前流过变压器的初级线圈的初级侧电流进行判断。

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