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1. 发明授权

US5225343A Process for preparation of an adventive embryo of podophyllum 失效
标题翻译：制备PODOPHYLLUM的先进的EMBRYO的方法
公开(公告)号：US5225343A
公开(公告)日：1993-07-06
申请号：US603361
申请日：1990-10-26
申请人： Ko Sakata , Eitaroh Morita , Tetsuya Takezono
发明人： Ko Sakata , Eitaroh Morita , Tetsuya Takezono
IPC分类号： A01H4/00 , C12N5/00 , C12N5/04 , C12P17/18
CPC分类号： C12N5/04 , A01H4/00 , C12N5/0025 , C12P17/181
摘要： A process for the preparation of an adventive embryo of a plant belonging to the genus Podophyllum, comprising the steps of preparing cells of a plant belonging to the genus Podophyllum, incubating the cells in a sugar free medium or a medium containing up to 2% by weight sugar, and then incubating the cells in a medium containing more than 2% by weight of sugar to generate an adventive embryo; a process for the production of a podophyllotoxin compound comprising the steps of preparing an adventive embryo of a plant belonging to the genus Podophyllum, culturing the adventive embryo to generate an adventitious organ, culturing the adventitious organ to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound; and a process for the production of a podophyllotoxin compound, comprising the steps of preparing de-differentiated cells or tissue of a plant belonging to the genus Podophyllum culturing the de-differentiated cells or tissue in a liquid medium to generate a differentiated organ, culturing the differentiated organ on a solid medium to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound.

2. 发明授权

US5336605A Production of podophyllotoxins using podophyllum 失效
标题翻译：使用鬼臼鱼生产鬼臼毒素
公开(公告)号：US5336605A
公开(公告)日：1994-08-09
申请号：US11671
申请日：1993-02-01
申请人： Ko Sakata , Eitaroh Morita , Tetsuya Takezono
发明人： Ko Sakata , Eitaroh Morita , Tetsuya Takezono
IPC分类号： A01H4/00 , C12N5/00 , C12N5/04 , C12P17/18 , C12N5/02
CPC分类号： C12N5/04 , A01H4/00 , C12N5/0025 , C12P17/181
摘要： A process for the preparation of an adventive embryo of a plant belonging to the genus Podophyllum, comprising the steps of preparing cells of a plant belonging to the genus Podophyllum, incubating the cells in a sugar free medium or a medium containing up to 2% by weight sugar, and then incubating the cells in a medium containing more than 2% by weight of sugar to generate an adventive embryo; a process for the production of a podophyllotoxin compound comprising the steps of preparing an adventive embryo of a plant belonging to the genus Podophyllum, culturing the adventive embryo to generate an adventitious organ, culturing the adventitious organ to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound; and a process for the production of a podophyllotoxin compound, comprising the steps of preparing de-differentiated cells or tissue of a plant belonging to the genus Podophyllum culturing the de-differentiated cells or tissue in a liquid medium to generate a differentiated organ, culturing the differentiated organ on a solid medium to produce a podophyllotoxin compound, and recovering the podophyllotoxin compound.
摘要翻译：一种制备属于Podophyllum属的植物的外来胚胎的方法，包括以下步骤：制备属于Podophyllum属植物的细胞，将无细胞培养基中的细胞或含有高达2％的培养基重量糖，然后将细胞在含有超过2重量％糖的培养基中培养以产生外来胚胎; 一种生产鬼臼毒素化合物的方法，包括以下步骤：制备属于Podophyllum属植物的外来胚胎，培养外来胚胎以产生外来器官，培养外来器官以产生鬼臼毒素化合物，并回收鬼臼毒素复合; 以及生产鬼臼毒素化合物的方法，包括以下步骤：在液体培养基中培养属于茯苓属的植物的去分化细胞或组织培养分化的细胞或组织以产生分化的器官，培养分离的器官在固体培养基上产生鬼臼毒素化合物，并回收鬼臼毒素化合物。

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