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1. 发明申请

US20100333220A1 HUMAN TISSUE FACTOR-PRODUCING KNOCK-IN NON-HUMAN ANIMAL 失效
标题翻译：人体组织因子生产非人类动物
公开(公告)号：US20100333220A1
公开(公告)日：2010-12-30
申请号：US12461453
申请日：2009-08-12
申请人： Kiyoshi Habu , Kou-ichi Jishage , Hideki Adachi , Naohiro Yabuta
发明人： Kiyoshi Habu , Kou-ichi Jishage , Hideki Adachi , Naohiro Yabuta
IPC分类号： C12P21/00
CPC分类号： C12N15/8509 , A01K67/0271 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/072 , A01K2217/075 , A01K2227/105 , A01K2267/03 , C12N2800/30
摘要： A non-human animal that produces human tissue factor (TF) without substantially producing non-human animal tissue factor, said animal having a genome in which cDNA encoding human TF has been inserted upstream of the translation initiation codon for the non-human animal genomic TF gene.
摘要翻译：所述动物具有编码人TF的cDNA已被插入非人动物基因组的翻译起始密码子的上游的基因组，所述非人动物产生人组织因子（TF），而基本上不产生非人动物组织因子 TF基因。

2. 发明授权

US08067665B2 Methods of using human tissue factor-producing knock-in mice 失效
标题翻译：使用人组织因子产生敲入小鼠的方法
公开(公告)号：US08067665B2
公开(公告)日：2011-11-29
申请号：US12461453
申请日：2009-08-12
申请人： Kiyoshi Habu , Kou-ichi Jishage , Hideki Adachi , Naohiro Yabuta
发明人： Kiyoshi Habu , Kou-ichi Jishage , Hideki Adachi , Naohiro Yabuta
IPC分类号： C12P21/00
CPC分类号： C12N15/8509 , A01K67/0271 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/072 , A01K2217/075 , A01K2227/105 , A01K2267/03 , C12N2800/30
摘要： A non-human animal that produces human tissue factor (TF) without substantially producing non-human animal tissue factor, said animal having a genome in which cDNA encoding human TF has been inserted upstream of the translation initiation codon for the non-human animal genomic TF gene.
摘要翻译：所述动物具有编码人TF的cDNA已被插入非人动物基因组的翻译起始密码子的上游的基因组，所述非人动物产生人组织因子（TF），而基本上不产生非人动物组织因子 TF基因。

3. 发明授权

US07592502B2 Knock-in non-human animal producing human tissue factor 失效
标题翻译：敲入非人类动物产生人体组织因子
公开(公告)号：US07592502B2
公开(公告)日：2009-09-22
申请号：US10478058
申请日：2002-05-17
申请人： Kiyoshi Habu , Kou-ichi Jishage , Hideki Adachi , Naohiro Yabuta
发明人： Kiyoshi Habu , Kou-ichi Jishage , Hideki Adachi , Naohiro Yabuta
IPC分类号： A01K67/027 , C12N15/00 , C12N15/87 , C12N5/00 , C12N5/02
CPC分类号： C12N15/8509 , A01K67/0271 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/072 , A01K2217/075 , A01K2227/105 , A01K2267/03 , C12N2800/30
摘要： A non-human animal that produces human tissue factor (TF) without substantially producing non-human animal tissue factor, said animal having a genome in which cDNA encoding human TF has been inserted upstream of the translation initiation codon for the non-human animal genomic TF gene.
摘要翻译：所述动物具有编码人TF的cDNA已被插入非人动物基因组的翻译起始密码子的上游的基因组，所述非人动物产生人组织因子（TF），而基本上不产生非人动物组织因子 TF基因。

4. 发明申请

US20100329988A1 Method for Identifying Within a Mammal a DNA Encoding A Physiologically Active Polypeptide 审中-公开
标题翻译：用于在哺乳动物中识别编码生理活性多肽的DNA的方法
公开(公告)号：US20100329988A1
公开(公告)日：2010-12-30
申请号：US12858960
申请日：2010-08-18
申请人： Kiyoshi Habu , Kou-ichi Jishage , Hiroshi Suzuki
发明人： Kiyoshi Habu , Kou-ichi Jishage , Hiroshi Suzuki
IPC分类号： A61K49/00 , A61P43/00
CPC分类号： A01K67/0275 , A01K67/0271 , A01K2217/05 , A61K38/00 , A61K48/00 , A61K2039/53 , C12N15/1034 , G01N33/5088
摘要： The present inventors found that a physiological effect of a particular DNA can be detected independently within mice into which a pool of various DNAs in various quantities has been introduced. This finding suggests that it is possible to identify a DNA having a particular physiological effect by successively fractionating a pool of various DNAs in various quantities using the particular physiological effect seen within a mammal as an index. Such a method of screening will have the advantage of saving much time and effort as required in conventional screenings such as those utilizing transgenic and knockout mice. Furthermore, the method of screening has the additional advantage of enabling the identification of a DNA having a physiological activity, for example, even when the cells producing a physiologically active substance cannot be maintained in vitro or in immunodeficient animals, or when the cells change their characteristics during passage and stop producing the physiologically active substance.
摘要翻译：本发明人发现，在引入了各种量的各种DNA的小鼠中，可以独立地检测特定DNA的生理作用。该发现表明，可以通过使用哺乳动物中看到的特定生理效应作为指标，以各种量依次分级各种DNA的池来鉴定具有特定生理效应的DNA。这种筛选方法将具有如常规筛选（例如使用转基因和敲除小鼠的筛选）所需的大量时间和精力的优点。此外，筛选方法具有能够鉴定具有生理活性的DNA的额外优点，例如即使当生产生理活性物质的细胞不能在体外或免疫缺陷动物中维持时，或者当细胞改变其特性在通过和停止生产生理活性物质。

5. 发明申请

US20090092995A1 Method for Identifying Within a Mammal a DNA encoding a Physiologically Active Polypeptide 审中-公开
标题翻译：用于在哺乳动物体内鉴定编码生理活性多肽的DNA的方法
公开(公告)号：US20090092995A1
公开(公告)日：2009-04-09
申请号：US12327770
申请日：2008-12-03
申请人： Kiyoshi Habu , Kou-Ichi Jishage , Hiroshi Suzuki
发明人： Kiyoshi Habu , Kou-Ichi Jishage , Hiroshi Suzuki
IPC分类号： C12Q1/68
CPC分类号： A01K67/0275 , A01K67/0271 , A01K2217/05 , A61K38/00 , A61K48/00 , A61K2039/53 , C12N15/1034 , G01N33/5088
摘要： The present inventors found that a physiological effect of a particular DNA can be detected independently within mice into which a pool of various DNAs in various quantities has been introduced. This finding suggests that it is possible to identify a DNA having a particular physiological effect by successively fractionating a pool of various DNAs in various quantities using the particular physiological effect seen within a mammal as an index. Such a method of screening will have the advantage of saving much time and effort as required in conventional screenings such as those utilizing transgenic and knockout mice. Furthermore, the method of screening has the additional advantage of enabling the identification of a DNA having a physiological activity, for example, even when the cells producing a physiologically active substance cannot be maintained in vitro or in immunodeficient animals, or when the cells change their characteristics during passage and stop producing the physiologically active substance.
摘要翻译：本发明人发现，在引入了各种量的各种DNA的小鼠中，可以独立地检测特定DNA的生理作用。该发现表明，可以通过使用哺乳动物中看到的特定生理效应作为指标，以各种量依次分级各种DNA的池来鉴定具有特定生理效应的DNA。这种筛选方法将具有如常规筛选（例如使用转基因和敲除小鼠的筛选）所需的大量时间和精力的优点。此外，筛选方法具有能够鉴定具有生理活性的DNA的额外优点，例如即使当生产生理活性物质的细胞不能在体外或免疫缺陷动物中维持时，或者当细胞改变其特性在通过和停止生产生理活性物质。

6. 发明申请

US20090288177A1 SGRF GENE-MODIFIED MOUSE 有权
标题翻译： SGRF基因修饰鼠
公开(公告)号：US20090288177A1
公开(公告)日：2009-11-19
申请号：US12499694
申请日：2009-07-08
申请人： Kiyoshi Habu , Yuichi Hirata
发明人： Kiyoshi Habu , Yuichi Hirata
IPC分类号： A01K67/027
CPC分类号： A01K67/0276 , A01K67/0271 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0325 , A01K2267/0368 , C12N15/8509 , C12N2800/30
摘要： A targeting vector was constructed by replacing exon regions in the SGRF gene with appropriate drug marker genes. This vector was transfected into mouse ES cell lines to obtain chimeric mice, which were then crossed with C57BL/6J mice to obtain mice comprising cells in which one SGRF gene alleles was inactivated. By crossing these mice with each other, the present inventors succeeded in producing mice in which both SGRF gene alleles were inactivated. These genetically modified animals can be used to predict the side effects of drugs such as SGRF antagonists.
摘要翻译：通过用适当的药物标记基因取代SGRF基因中的外显子区域构建靶向载体。将该载体转染入小鼠ES细胞系以获得嵌合小鼠，然后将其与C57BL / 6J小鼠杂交，以获得包含其中一个SGRF基因等位基因被灭活的细胞的小鼠。通过使这些小鼠相互交叉，本发明人成功地生产了两种SGRF基因等位基因都被灭活的小鼠。这些转基因动物可用于预测药物如SGRF拮抗剂的副作用。

7. 发明授权

US07829757B2 SGRF gene-modified mouse 有权
标题翻译： SGRF基因修饰小鼠
公开(公告)号：US07829757B2
公开(公告)日：2010-11-09
申请号：US12499694
申请日：2009-07-08
申请人： Kiyoshi Habu , Yuichi Hirata
发明人： Kiyoshi Habu , Yuichi Hirata
IPC分类号： G01N33/00
CPC分类号： A01K67/0276 , A01K67/0271 , A01K2217/075 , A01K2227/105 , A01K2267/03 , A01K2267/0325 , A01K2267/0368 , C12N15/8509 , C12N2800/30
摘要： A targeting vector was constructed by replacing exon regions in the SGRF gene with appropriate drug marker genes. This vector was transfected into mouse ES cell lines to obtain chimeric mice, which were then crossed with C57BL/6J mice to obtain mice comprising cells in which one SGRF gene alleles was inactivated. By crossing these mice with each other, the present inventors succeeded in producing mice in which both SGRF gene alleles were inactivated. These genetically modified animals can be used to predict the side effects of drugs such as SGRF antagonists.
摘要翻译：通过用适当的药物标记基因取代SGRF基因中的外显子区域构建靶向载体。将该载体转染入小鼠ES细胞系以获得嵌合小鼠，然后将其与C57BL / 6J小鼠杂交，以获得包含其中一个SGRF基因等位基因被灭活的细胞的小鼠。通过使这些小鼠相互交叉，本发明人成功地生产了两种SGRF基因等位基因都被灭活的小鼠。这些转基因动物可用于预测药物如SGRF拮抗剂的副作用。

8. 发明申请

US20090061485A1 Method of Producing an Antibody Using a Cell in Which the Function of Fucose Transporter Is Inhibited 审中-公开
标题翻译：使用抑制岩藻糖转运蛋白功能的细胞产生抗体的方法
公开(公告)号：US20090061485A1
公开(公告)日：2009-03-05
申请号：US11793712
申请日：2004-12-22
申请人： Masayuki Tsuchiya , Shigeyuki Iijima , Izumi Sugo , Yasuo Sekimori , Kiyoshi Habu , Masamichi Sugimoto
发明人： Masayuki Tsuchiya , Shigeyuki Iijima , Izumi Sugo , Yasuo Sekimori , Kiyoshi Habu , Masamichi Sugimoto
IPC分类号： C12P21/08 , C12N5/06 , C12N5/10 , C12P21/02
CPC分类号： C12N9/1081 , A01K2217/075 , A01K2227/10 , C07K16/00 , C07K2317/41
摘要： The present invention relates to a method of producing a recombinant protein, particularly an antibody, using a cell in which the function of a fucose transporter is inhibited, and it also provides a cell in which the expression of fucose transporter genes on both homologous chromosomes is artificially suppressed.
摘要翻译：本发明涉及使用其中岩藻糖转运蛋白的功能被抑制的细胞产生重组蛋白质，特别是抗体的方法，并且还提供了在两个同源染色体上表达岩藻糖转运蛋白基因的细胞是人为压制

9. 发明授权

US08298816B2 Expression systems using mammalian beta-actin promoter 失效
标题翻译：使用哺乳动物β-肌动蛋白启动子的表达系统
公开(公告)号：US08298816B2
公开(公告)日：2012-10-30
申请号：US10581183
申请日：2004-12-03
申请人： Hiroyuki Tsunoda , Kiyoshi Habu
发明人： Hiroyuki Tsunoda , Kiyoshi Habu
IPC分类号： C12N15/00 , C12N5/00 , C12P21/06
CPC分类号： G06Q50/22 , A01K2217/05 , C07K14/82 , C12N15/85 , C12N2830/00 , C12N2830/48 , C12N2830/85 , Y02A90/22
摘要： Promoter activities were examined by comparing combinations of promoters and enhancers derived from various genes. A hybrid promoter comprising a combination of a CMV enhancer and a mammalian β-actin promoter, or the post-transcriptional regulatory region of the genomic sequence Woodchuck Hepatitis Virus (WPRE) and a mammalian β-actin promoter was found to be stronger than existing promoters. Furthermore, the activities of the β-actin promoters could be enhanced by coexpressing the oncogene product Ras, which is a transactivator.
摘要翻译：通过比较来自各种基因的启动子和增强子的组合来检查启动子活性。发现包含CMV增强子和哺乳动物 - 肌动蛋白启动子或基因组序列Woodchuck Hepatitis Virus（WPRE）的转录后调控区和哺乳动物和肌动蛋白启动子的组合的杂合启动子比现有发起人。此外，可以通过共表达作为反式激活因子的致癌基因产物Ras来增强促进剂的活性。

10. 发明申请

US20060246456A1 Fucose transporter 失效
标题翻译：岩藻糖转运蛋白
公开(公告)号：US20060246456A1
公开(公告)日：2006-11-02
申请号：US10561191
申请日：2004-06-18
申请人： Masayuki Tsuchiya , Shigeyuki Iijima , Izumi Sugo , Yasuo Sekimori , Kenju Ueno , Kiyoshi Habu
发明人： Masayuki Tsuchiya , Shigeyuki Iijima , Izumi Sugo , Yasuo Sekimori , Kenju Ueno , Kiyoshi Habu
IPC分类号： C12Q1/68 , G01N33/53 , C07H21/04 , C12P21/06 , C07K16/28 , C07K14/705
CPC分类号： C07K14/47 , C12P21/005 , G01N2500/00
摘要： The present invention provides a gene encoding a fucose transporter, a fucose transporter polypeptide, a method for screening for a compound that binds to a fucose transporter or a compound that inhibits fucose transport activity, a cell having inhibited fucose transporter functions, and a cell wherein the expression of the fucose transporter is inhibited. The present invention further relates to a method for producing recombinant protein, and specifically, to a method for producing protein by which fucose existing within the Golgi apparatus of a host cell is decreased, a method for inhibiting the addition of fucose to protein by which fucose existing within the Golgi apparatus of a host cell is decreased upon production of recombinant protein using the host cell, a method for increasing the cytotoxic activity of an antibody by which an antibody is produced using a cell wherein fucose existing within the Golgi apparatus is decreased, and a cell having a Golgi apparatus wherein fucose existing within the Golgi apparatus is decreased.
摘要翻译：本发明提供了编码岩藻糖转运蛋白，岩藻糖转运蛋白多肽，筛选结合岩藻糖转运蛋白的化合物或抑制岩藻糖转运活性的化合物的方法，具有抑制岩藻糖转运蛋白功能的细胞的基因，以及细胞，其中岩藻糖转运蛋白的表达被抑制。本发明还涉及一种生产重组蛋白的方法，具体地说涉及一种生物蛋白质的制造方法，其中存在于宿主细胞高尔基体内的岩藻糖减少，一种抑制岩藻糖加入蛋白质的方法，通过该方法将岩藻糖在使用宿主细胞产生重组蛋白质时，存在于宿主细胞的高尔基体内的存在的方法，使用其中存在于高尔基体中的岩藻糖减少的细胞增加抗体产生抗体的方法，和具有高尔基体的细胞，其中存在于高尔基体内的岩藻糖减少。

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