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    • 7. 发明授权
    • Compositions and methods for the preservation of living tissues
    • 用于保存活组织的组合物和方法
    • US5962213A
    • 1999-10-05
    • US989470
    • 1997-12-12
    • Philippa M. WigginsAlexander B. FergusonJames D. Watson
    • Philippa M. WigginsAlexander B. FergusonJames D. Watson
    • A01N1/02A01N3/00C12N1/04
    • A01N1/0226A01N1/02A01N1/0221C12N1/04
    • The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of dihydrogen phosphate, bicarbonate, nitrate bisulfate and iodide. In one embodiment, the solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3 M, a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties, with the first neutral solute preferably being selected from the group consisting of raffinose and trehalose and the second neutral solute preferably being selected from the group consisting of trimethyl amine oxide and betaine. For the preservation of many living biological materials, the inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.
    • 本发明提供了用于保存活体生物材料的方法和方法,其使器官,组织和细胞能够以最小的生物活性损失被长时间储存​​。 本发明溶液与待保存的生物材料基本上是等渗的,并且基本上不含磷酸二氢盐,碳酸氢盐,硝酸盐硫酸氢盐和碘化物。 在一个实施方案中,溶液包含具有至少约335分子量和至少约0.3M的在水中的溶解度的第一中性溶质,分子量小于约200的第二中性溶质,并且具有亲水性和 疏水部分,第一中性溶质优选选自棉子糖和海藻糖,第二中性溶质优选选自三甲胺氧化物和甜菜碱。 为了保存许多活的生物材料,本发明的溶液优选含有选自硫酸钙和氯化钙的钙盐。