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    • 6. 发明授权
    • Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays
    • 用于将生物分子附着到固体支持物,水凝胶和水凝胶阵列的方法和组合物
    • US06372813B1
    • 2002-04-16
    • US09344620
    • 1999-06-25
    • Travis JohnsonJohn McGowenAllyson BeuhlerCharles Kimball BrushRobert Emil Lajos
    • Travis JohnsonJohn McGowenAllyson BeuhlerCharles Kimball BrushRobert Emil Lajos
    • C08J328
    • G01N33/54353
    • The present invention provides solid supports (e.g., glass) and polymer hydrogels (particularly polymer hydrogel arrays present on a solid support) comprising one or more reactive sites for the attachment of biomolecules, as well as biomolecules comprising one or more reactive sites for attachment to solid supports and polymer hydrogels. The invention further provides novel compositions and methods for the preparation of biomolecules, solid supports, and polymer hydrogels comprising reactive sites. The invention also provides for preparation of crosslinked solid supports, polymer hydrogels, and hydrogel arrays, wherein one or more biomolecules is attached by means of the reactive sites in a photocycloaddition reaction. Advantageously, according to the invention, crosslinking of the hydrogel and attachment of biomolecules can be done in a single step.
    • 本发明提供固体支持物(例如玻璃)和聚合物水凝胶(特别是存在于固体支持物上的聚合物水凝胶阵列),其包含用于附着生物分子的一个或多个反应性位点,以及包含一个或多个反应性位点的生物分子, 固体支持物和聚合物水凝胶。 本发明还提供了用于制备生物分子,固体支持物和包含反应性位点的聚合物水凝胶的新型组合物和方法。 本发明还提供了交联固体载体,聚合物水凝胶和水凝胶阵列的制备,其中通过光环加成反应中的反应性位点连接一个或多个生物分子。 有利地,根据本发明,水凝胶的交联和生物分子的连接可以在单一步骤中进行。