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    • 1. 发明授权
    • Liquid film type, anion-selective electrode
    • 液膜型,阴离子选择性电极
    • US4519891A
    • 1985-05-28
    • US574587
    • 1984-01-27
    • Kenshi SugaharaKazuo YasudaJunji Mori
    • Kenshi SugaharaKazuo YasudaJunji Mori
    • G01N27/327G01N27/333G01N27/30
    • G01N27/3335
    • A liquid film type, anion-selective electrode of the present invention is suitable for measuring a chloride ion concentration of blood. A sensitive film contains a plurality of plasticizers having different dielectric constants, an anion-sensitive substance and a polymeric support material for supporting them. An appropriate content of o-nitrophenyloctylether having a high dielectric constant is 5-30% by weight, an appropriate content of n-tetradecyl alcohol having a low dielectric constant 5-40% by weight, and an appropriate content of dimethyldioctadecylammonium chloride as the anion-sensitive substance is 5-30% by weight. An electrode for measuring anions with such a sensitive film has less adverse affect by protein on measurements. The sensitive film further containing n-dodecyl alcohol has a much smaller error of measurement.
    • 本发明的液膜型阴离子选择性电极适用于测定血液的氯离子浓度。 敏感膜含有多种具有不同介电常数的增塑剂,阴离子敏感物质和用于支撑它们的聚合物载体材料。 具有高介电常数的邻硝基苯基辛基醚的适当含量为5-30重量%,适当含量的介电常数低于5-40重量%的正十四烷基醇和适当含量的二甲基十二烷基氯化铵作为阴离子 敏感物质为5-30重量%。 用这种敏感膜测量阴离子的电极具有较少的蛋白质对测量的不利影响。 进一步含有正十二烷醇的敏感膜具有小得多的测量误差。
    • 5. 发明授权
    • Electrochemical sensor having three layer membrane containing
immobilized enzymes
    • 具有含有固定化酶的三层膜的电化学传感器
    • US4795707A
    • 1989-01-03
    • US802193
    • 1985-11-25
    • Yasusi NiiyamaKenshi Sugahara
    • Yasusi NiiyamaKenshi Sugahara
    • G01N27/327C12Q1/00G01N27/30C12M1/40C12N11/02C12N11/14G01N27/26
    • C12Q1/005Y10S435/817
    • An electrochemical sensor is formed having a working electrode for detecting hydrogen peroxide surrounded by a cylinder portion, and with an enzymecontaining membrane at its tip. The membrane has a porous layer permeable to hydrogen peroxide between a layer containing an immobilized enzyme capable of decomposing hydrogen peroxide and a layer containing an immobilized enzyme capable of decomposing a substrate to form hydrogen peroxide. The cylinder portion is embedded in the layer containing the hydrogen peroxide decomposing enzyme and surrounds the working electrode such that the electrode is in contact with the porous layer but is not in contact with the layer containing the hydrogen peroxide decomposing enzyme. The layer containing the hydrogen peroxide forming enzyme is on a side of the porous layer opposite the electrode so as not to contact the electrode. Activity of the hydrogen peroxide decomposing enzyme is no more than one-fourth of the activity of the hydrogen peroxide forming enzyme. The electrochemical sensor reduces base line elevation after measurement action has been discontinued and measurement of a next sample is restarted.
    • 形成电化学传感器,其具有用于检测由圆筒部分包围的过氧化氢的工作电极,以及在其末端具有酶的膜。 该膜具有在含有能够分解过氧化氢的固定化酶的层和含有能够分解底物以形成过氧化氢的固定化酶的层之间的可透过过氧化氢的多孔层。 圆筒部嵌入含有过氧化氢分解酶的层中,并且包围工作电极,使得电极与多孔层接触,但不与含有过氧化氢分解酶的层接触。 含有过氧化氢形成酶的层在与电极相对的多孔层的一侧上,以便不与电极接触。 过氧化氢分解酶的活性不超过过氧化氢形成酶活性的四分之一。 测量动作停止后,电化学传感器降低基线高度,并重新开始下一个样品的测量。