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1. 发明申请

US20120053069A1 Determination of a Genotype of an Amplification Product at Multiple Allelic Sites 审中-公开
标题翻译：确定多个等位基因位点扩增产物的基因型
公开(公告)号：US20120053069A1
公开(公告)日：2012-03-01
申请号：US12618686
申请日：2009-11-13
申请人： Kenneth J. LIVAK , Federico Goodsaid
发明人： Kenneth J. LIVAK , Federico Goodsaid
IPC分类号： C40B30/04 , C40B40/06 , C12Q1/68
CPC分类号： C12Q1/6858 , C12Q1/6818 , C12Q2561/101 , C12Q2537/143 , C12Q2563/107 , C12Q2531/119
摘要： A method is provided for genotyping a target sequence at at least two allelic sites by a 5′ nuclease amplification reaction. In one embodiment, the method includes performing a nucleic acid amplification on a target sequence having at least two different allelic sites using a nucleic acid polymerase having 5′-3′ nuclease activity and a primer capable of hybridizing to the target sequence in the presence of two or more sets of allelic oligonucleotide probes wherein at least all but one of the allelic oligonucleotide probes include a different fluorescer than the other probes and a quencher positioned on the probe to quench the fluorescence of the fluorescer; detecting a fluorescence spectrum of the amplification; calculating a fluorescence contribution of each fluorescer to the fluorescence spectrum; and determining a presence or absence of the different allelic variants based on the fluorescence contribution of each fluorescer to the combined fluorescence spectrum.
摘要翻译：提供了一种通过5'核酸酶扩增反应在至少两个等位基因位点对靶序列进行基因分型的方法。在一个实施方案中，该方法包括使用具有5'-3'核酸酶活性的核酸聚合酶和能够与靶序列杂交的引物在具有至少两个不同等位基因位点的靶序列上进行核酸扩增两组或多组等位基因寡核苷酸探针，其中等位基因寡核苷酸探针中的至少全部除一个之外包括与其它探针不同的荧光剂，以及位于探针上的猝灭剂以淬灭荧光剂的荧光; 检测扩增的荧光光谱; 计算每个荧光剂对荧光光谱的荧光贡献; 并且基于每个荧光剂对组合的荧光光谱的荧光贡献来确定不同等位基因变体的存在或不存在。

2. 发明申请

US20120303472A1 METHODS FOR PLACING, ACCEPTING, AND FILLING ORDERS FOR PRODUCTS AND SERVICES 有权
标题翻译：用于产品和服务的配置，接受和填充订单的方法
公开(公告)号：US20120303472A1
公开(公告)日：2012-11-29
申请号：US13458879
申请日：2012-04-27
申请人： Ryan T. KOEHLER , Kenneth J. LIVAK , Junko STEVENS , Francisco M. DE LA VEGA , Michael RHODES , Laurent R. BELLON , Janet S. ZIEGLE , Julie WILLIAMS , Dawn MADDEN , Dennis A. GILBERT , Charles R. SCAFE , Hadar I. Isaac , Yu N. WANG , Eugene G. SPIER , Xiaoqing YOU , Lily XU , Jeremy HEIL , Stephen GLANOWSKI , John SCOTT , Emily S. WINN-DEEN , Ivy MCMULLEN , Lini WU , Harold Carey GIRE , Michael W. HUNKAPILLER , Susan EDDINS , Leila G. SMITH
发明人： Ryan T. KOEHLER , Kenneth J. LIVAK , Junko STEVENS , Francisco M. DE LA VEGA , Michael RHODES , Laurent R. BELLON , Janet S. ZIEGLE , Julie WILLIAMS , Dawn MADDEN , Dennis A. GILBERT , Charles R. SCAFE , Hadar I. Isaac , Yu N. WANG , Eugene G. SPIER , Xiaoqing YOU , Lily XU , Jeremy HEIL , Stephen GLANOWSKI , John SCOTT , Emily S. WINN-DEEN , Ivy MCMULLEN , Lini WU , Harold Carey GIRE , Michael W. HUNKAPILLER , Susan EDDINS , Leila G. SMITH
IPC分类号： G06Q30/06
CPC分类号： C12Q1/6858 , B01L3/545 , B01L3/5453 , B01L2300/021 , C12Q2600/156 , C40B50/02 , G01N35/00663 , G06F19/20 , G06F19/22 , G06F19/26 , G06F19/28 , G06Q30/0621 , G06Q30/0633 , G06Q30/0641 , G06Q50/22 , C12Q2547/101
摘要： Methods and systems for ordering assays which detect SNPs or gene expression are provided. The methods use PCR and RT-PCR procedures. Collections of stock assays are assembled using pre- and post-manufacturing quality control procedures and made available to consumers via the Internet. In addition, custom assays are prepared upon order from the consumer and these assays are also prepared using pre- and post-manufacturing quality control procedures. The assays are then delivered to the consumer.
摘要翻译：提供了检测SNP或基因表达的排序方法和系统。方法采用PCR和RT-PCR方法。库存测定的集合使用制造前和制造后质量控制程序组装，并通过互联网提供给消费者。此外，根据消费者的命令制备定制测定，并且还使用制造前和制造后质量控制程序制备这些测定。然后将测定物递送给消费者。

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