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1. 发明授权

US06833449B1 Expression of the toxic portion of Cry1A in plants 失效
标题翻译： Cry1A在植物中的有毒部分的表达
公开(公告)号：US06833449B1
公开(公告)日：2004-12-21
申请号：US10394548
申请日：2003-03-21
申请人： Kenneth A. Barton , Michael J. Miller
发明人： Kenneth A. Barton , Michael J. Miller
IPC分类号： C12N1532
CPC分类号： C12N15/8216 , C12N15/8286 , Y02A40/162
摘要： Disclosed is a method for improving the expression of Cry1A in plants that makes use of codons preferentially used in native plant genes. The coding sequence of the gene for the Bacillus thuringiensis delta endotoxin Cry1A crystal protein was analyzed and found to have codons not preferred by plants. By constructing a synthetic protein coding sequence that uses codons which are preferred in plant genes, expression of the protein in plant cells was improved.
摘要翻译：公开了一种改善植物中Cry1A表达的方法，该方法利用优先用于天然植物基因的密码子。分析了苏云金芽孢杆菌δ内毒素Cry1A晶体蛋白基因的编码序列，发现植物不具有密码子。通过构建使用在植物基因中优选的密码子的合成蛋白质编码序列，提高了植物细胞中蛋白质的表达。

2. 发明授权

US5177308A Insecticidal toxins in plants 失效
标题翻译：杀虫剂在植物中的毒素
公开(公告)号：US5177308A
公开(公告)日：1993-01-05
申请号：US443425
申请日：1989-11-29
申请人： Kenneth A. Barton , Michael J. Miller
发明人： Kenneth A. Barton , Michael J. Miller
IPC分类号： A01H1/00 , A01H5/00 , A01H5/10 , A01N63/00 , A01N63/02 , C07K14/435 , C12N15/12 , C12N15/82
CPC分类号： C07K14/43522 , A01N63/02 , C07K14/43518 , C12N15/8286
摘要： Transgenic plants have been created which express an insect-specific toxin from a scorpion. The chimeric inheritable trait produced conditions of toxicity in the plant cells of toxicity to certain insects upon ingestion of plant tissues. The inheritable trait has also been cross-bred to plants transgenic to the Bacillus thuringiensis delta-endotoxin to produce plants having two independent insect-specific toxin traits. Insect feeding trails revealed additive toxic effects. A generalized approach for developing other insecticidal toxins as candidates for insertion into transgenic plants is also presented.

3. 发明授权

US5608142A Insecticidal cotton plants 失效
标题翻译：杀虫棉花植物
公开(公告)号：US5608142A
公开(公告)日：1997-03-04
申请号：US299767
申请日：1989-01-23
申请人： Kenneth A. Barton , Paul F. Umbeck
发明人： Kenneth A. Barton , Paul F. Umbeck
IPC分类号： A01H1/02 , C07K14/325 , C12N15/82 , A01H4/00
CPC分类号： C12N15/8286 , A01H1/02 , C07K14/325 , C12N15/8205
摘要： A plant expression vector is constructed to cause the expression of an amino-terminal portion of the Bacillus thuringiensis delta-endotoxin gene in plant cells and the vector is used to create transgenic plants expressing the toxin. A truncated form of the toxin is used, with carboxy-terminal prolines added for stability. A translational enhancer sequence derived from the untranslated leader sequence from the mRNA of the coat protein gene of alfalfa mosaic virus coat protein gene is placed between a promoter and the toxin gene to increase translational efficiency. The transgenic plants produced are toxic to Lepidopteran pests and can transmit that trait to their progeny by normal Mendelian inheritance.
摘要翻译：构建植物表达载体以引起苏云金芽孢杆菌δ-内毒素基因的氨基末端部分在植物细胞中的表达，并使用载体产生表达毒素的转基因植物。使用截短形式的毒素，加入羧基末端脯氨酸以获得稳定性。将来自苜蓿花叶病毒外壳蛋白基因的外壳蛋白基因的mRNA的非翻译前导序列的翻译增强子序列置于启动子和毒素基因之间以提高翻译效率。所产生的转基因植物对鳞翅目害虫是有毒的，并且可以通过孟德尔遗传传播这种特征到其后代。

4. 发明授权

US06617496B1 Effecting virus resistance in plants through the use of negative strand RNAs 失效
标题翻译：通过使用负链RNA来影响植物中的病毒抗性
公开(公告)号：US06617496B1
公开(公告)日：2003-09-09
申请号：US06788002
申请日：1985-10-16
申请人： Francis P. McCormick , Kenneth A. Barton , William F. Swain
发明人： Francis P. McCormick , Kenneth A. Barton , William F. Swain
IPC分类号： C12N1533
CPC分类号： C12N15/1131 , C12N15/1137 , C12N15/8218 , C12N2310/111 , C12Y105/01019
摘要： A strategy for effecting virus resistance in plants causes the transcription in the plant cells of negative RNA strands which are substantially complementary to a target RNA strand. The target RNA strand can be an mRNA transcript created in gene expression, a viral RNA, or other RNA present in the plant cells. The negative RNA strand is complementary to at least a portion of the target RNA strand to inhibit its activity in vivo.
摘要翻译：在植物中产生病毒抗性的策略导致与靶RNA链基本互补的负RNA链的植物细胞中的转录。靶RNA链可以是在基因表达中产生的mRNA转录物，病毒RNA或存在于植物细胞中的其他RNA。负RNA链与目标RNA链的至少一部分互补以抑制其在体内的活性。

5. 发明授权

US6037527A Expression of proteins in plants using an AMV coat protein leader sequence 失效
标题翻译：使用AMV外壳蛋白前导序列在植物中表达蛋白质
公开(公告)号：US6037527A
公开(公告)日：2000-03-14
申请号：US810720
申请日：1997-03-03
申请人： Kenneth A. Barton , Paul F. Umbeck
发明人： Kenneth A. Barton , Paul F. Umbeck
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8216 , C12N15/8286
摘要： A plant expression vector is constructed to cause the expression of an amino-terminal portion of the Bacillus thuringiensis delta-endotoxin gene in plant cells and the vector is used to create transgenic plants expressing the toxin. A truncated form of the toxin is used, with carboxy-terminal prolines added for stability. A translational enhancer sequence derived from the untranslated leader sequence from the mRNA of the coat protein gene of alfalfa mosaic virus coat protein gene is placed between a promoter and the toxin gene to increase translational efficiency. The transgenic plants produced are toxic to Lepidopteran pests and can transmit that trait to their progeny by normal Mendelian inheritance.
摘要翻译：构建植物表达载体以引起苏云金芽孢杆菌δ-内毒素基因的氨基末端部分在植物细胞中的表达，并使用载体产生表达毒素的转基因植物。使用截短形式的毒素，加入羧基末端脯氨酸以获得稳定性。将来自苜蓿花叶病毒外壳蛋白基因的外壳蛋白基因的mRNA的非翻译前导序列的翻译增强子序列置于启动子和毒素基因之间以提高翻译效率。所产生的转基因植物对鳞翅目害虫是有毒的，并且可以通过孟德尔遗传传播这种特征到其后代。

6. 发明授权

US5474925A Immobilized proteins in cotton fiber 失效
标题翻译：棉纤维中固定蛋白
公开(公告)号：US5474925A
公开(公告)日：1995-12-12
申请号：US217327
申请日：1994-03-23
申请人： John Maliyakal , Kenneth A. Barton
发明人： John Maliyakal , Kenneth A. Barton
IPC分类号： C12N15/82 , C12P21/04 , C12N15/00 , C12N5/14 , C12N15/52
CPC分类号： C12N15/8242
摘要： Transgenic cotton plants have been created which expressed an immobilized protein in the cotton fiber cells. The cotton fiber can be recovered from such transgenic cotton plants and then used as a substrate for fixing immobilized protein for use in industrial or laboratory processes. Using an enzyme expressed inside cotton fiber, it is possible to fabricate a convenient reaction column by simply packing the cotton fiber carrying the enzyme into a column and passing substrate therethrough.
摘要翻译：已经产生了在棉纤维细胞中表达固定化蛋白质的转基因棉花植物。棉纤维可以从这样的转基因棉花植物中回收，然后用作固定蛋白质用于工业或实验室工艺的底物。使用在棉纤维中表达的酶，可以通过简单地将携带酶的棉纤维填充到柱中并使基底通过其中来制备方便的反应柱。

7. 发明授权

US5015580A Particle-mediated transformation of soybean plants and lines 失效
标题翻译：大豆植物和线粒子介导的转化
公开(公告)号：US5015580A
公开(公告)日：1991-05-14
申请号：US193357
申请日：1988-05-12
申请人： Paul Christou , Dennis McCabe , William F. Swain , Kenneth A. Barton
发明人： Paul Christou , Dennis McCabe , William F. Swain , Kenneth A. Barton
IPC分类号： A01H1/00 , A01H5/00 , C12M3/00 , C12N5/02 , C12N15/09 , C12N15/82 , C12N15/87
CPC分类号： C12N15/87 , C12N15/8207
摘要： A method and apparatus is disclosed for the genetic transformation of soybean plants and plant lines by particle mediated transformation. Foreign genes are introduced into regenerable soybean tissues by coating on carrier particles which are physically accelerated into plant tissues. The treated plant tissues are then recovered and regenerated into whole sexually mature plants. The progeny are recovered from seed set by these plants and a portion of these progeny will contain in their genome the foreign gene. The procedure may be used to create novel genetically engineered soybean plants and lines.

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