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    • 6. 发明授权
    • Method of collecting highly pure polyhydroxyalkanoate from microbial cells
    • 从微生物细胞中收集高纯度聚羟基链烷酸酯的方法
    • US07393668B2
    • 2008-07-01
    • US10541389
    • 2004-01-20
    • Yoshifumi YanagitaNoriko OgawaYasuyoshi UedaFumio OsakadaKeiji Matsumoto
    • Yoshifumi YanagitaNoriko OgawaYasuyoshi UedaFumio OsakadaKeiji Matsumoto
    • C12P7/62C12N9/00
    • C12P7/625
    • The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles.The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.
    • 本发明的目的是提供一种分离纯化PHA的方法,而不会导致分子量的严重降低以获得高产率的高纯度PHA,其包括从培养的PHA有效除去PHA颗粒以外的细胞成分 含微生物细胞。 本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。 根据本发明的回收PHA的方法是通过对含PHA的微生物细胞的水性悬浮液进行物理破坏处理和低温碱添加来有效地破坏细胞以回收PHA的方法, 然后用酶和/或表面活性剂处理PHA。 此外,通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集,可以扩大PHA的粒径。
    • 7. 发明申请
    • Method of collecting highly pure polyhydroxyalkanoate from microbial cells
    • 从微生物细胞中收集高纯度聚羟基链烷酸酯的方法
    • US20060084161A1
    • 2006-04-20
    • US10541389
    • 2004-01-20
    • Yoshifumi YanagitaNoriko OgawaYasuyoshi UedaFumio OsakadaKeiji Matsumoto
    • Yoshifumi YanagitaNoriko OgawaYasuyoshi UedaFumio OsakadaKeiji Matsumoto
    • C12N9/00
    • C12P7/625
    • The present invention has an object to provide a method for separating and purifying a PHA without causing a serious decrease of the molecular weight to obtain a highly pure PHA in a high yield, which comprises efficiently removing cell components other than PHA particles from a cultured PHA-containing microbial cell. Another object of the present invention is to provide a method for obtaining an agglomerate of PHA particles. The method for recovering a PHA according to the present invention is a method which comprises efficiently disrupting a cell to recover the PHA by carrying out a physical disruption treatment and an alkali addition at low temperature for an aqueous suspension of the PHA-containing microbial cell, and then treating the PHA with an enzyme and/or a surfactant. Moreover, the particle diameter of the PHA may be enlarged by suspending the PHA in a hydrophilic solvent and/or water, and stirring at a temperature equal to or below the boiling point of said suspension, to agglomerate said PHA.
    • 本发明的目的是提供一种分离纯化PHA的方法,而不会导致分子量的严重降低以获得高产率的高纯度PHA,其包括从培养的PHA有效除去PHA颗粒以外的细胞成分 含微生物细胞。 本发明的另一个目的是提供一种获得PHA颗粒附聚物的方法。 根据本发明的回收PHA的方法是通过对含PHA的微生物细胞的水性悬浮液进行物理破坏处理和低温碱添加来有效地破坏细胞以回收PHA的方法, 然后用酶和/或表面活性剂处理PHA。 此外,通过将PHA悬浮在亲水溶剂和/或水中并在等于或低于所述悬浮液沸点的温度下搅拌以使所述PHA聚集,可以扩大PHA的粒径。
    • 8. 发明授权
    • Chloroprene polymer latex and process for producing the same
    • 氯丁橡胶聚合物胶乳及其制造方法
    • US08586670B2
    • 2013-11-19
    • US12439424
    • 2007-08-30
    • Youichiro TakenoshitaNoriko Ogawa
    • Youichiro TakenoshitaNoriko Ogawa
    • C08L11/02C08L11/00C08L9/10C08L25/02C08L9/00C08L47/00C08F214/02C08F36/18C08F136/18
    • C09J111/02C08F36/16C08K3/22C08K5/13C08L11/02C08F2/26
    • A latex of a chloroprene polymer formed from one or more monomers comprising 2-chloro-1,3-butadiene as the main monomer ingredient, characterized in that the solid matter in the polymer latex has a gel content less than 3 mass %, the solid matter in the polymer latex includes a tetrahydrofuran-soluble component having a weight-average molecular weight of from 550,000 to 1,100,000 and a molecular-weight distribution (Mw/Mn) of from 2.0 to 3.1, and the dried solid matter in the polymer latex has a crystallization rate (R) represented by hardness increase at −10° C. (R is the time required for initial hardness (time=0) to increase by 30 points at −10° C. as measured according to JIS-K6301) less than 60 minutes. The chloroprene polymer latex has an excellent balance among initial bond strength, contact adhesion, and water resistance. This latex is useful as an adhesive and can be used in applications where heat resistance of 60° C. or higher, initial bond strength, and contact adhesion are required.
    • 由一种或多种包含2-氯-1,3-丁二烯作为主要单体成分的单体形成的氯丁二烯聚合物的胶乳,其特征在于聚合物胶乳中的固体物质的凝胶含量小于3质量%,固体 聚合物胶乳中的物质包括重均分子量为550,000至1,100,000且分子量分布(Mw / Mn)为2.0至3.1的四氢呋喃可溶组分,并且聚合物胶乳中的干燥固体物质具有 在-10℃下由硬度增加表示的结晶速率(R)(R是根据JIS-K6301测量的初始硬度(时间= 0)在-10℃下增加30点所需的时间) 超过60分钟。 氯丁二烯聚合物胶乳在初始粘合强度,接触粘附性和耐水性方面具有优异的平衡。 该胶乳可用作粘合剂,并且可用于需要耐热性为60℃或更高,初始粘结强度和接触粘合性的应用中。