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    • 7. 发明授权
    • Intra-liquid particle classification apparatus using light scattering
    • 使用光散射的液体内颗粒分级装置
    • US5517870A
    • 1996-05-21
    • US168509
    • 1993-12-22
    • Masaaki KurimuraMasaaki Hanawa
    • Masaaki KurimuraMasaaki Hanawa
    • G01N15/14G01N21/15G01N33/49G01N15/00
    • G01N15/1404
    • A liquid sample is sucked from a staining vessel by a first sampling nozzle and the liquid sample is discharged into a flow cell unit, the liquid sample being supplied to the staining vessel by a second sampling nozzle so as to be stained in the staining vessel in advance. When the liquid sample is being discharged through the first sampling nozzle, a sheath liquid is supplied to a flow cell so that the sheath liquid flows with the liquid sample included therein. In this condition, passage of particles in the flow cell is detected by an optical system for detection at a still frame image pick-up position, and after a predetermined delay time based on the detection, strobe light is radiated so as to pick up a still frame image due to the scattered light to thereby perform classification and counting of the particles. Foreign matter in the liquid sample discharged from the sampling nozzle is caught by a filter and exhausted by the sheath liquid which overflows to the upper portion of the filter after completion of discharge of the liquid sample from the sampling nozzle. Bubbles in the flow cell are removed through an air vent and bubbles in the sheath liquid are removed after once reserved in an air vent chamber. Thus, foreign matter/bubbles in the liquid sample/sheath liquid can be prevented from entering the flow cell unit to make it possible to perform testing with high accuracy without lowering testing efficiency.
    • 通过第一采样喷嘴从染色容器吸取液体样品,并将液体样品排出到流动池单元中,通过第二取样喷嘴将液体样品供应到染色容器中,以在染色容器中染色 提前。 当通过第一采样喷嘴排出液体样品时,将鞘液供给到流动池,使得鞘液与其中包含的液体样品一起流动。 在这种情况下,通过用于在静止帧拍摄位置检测的光学系统来检测流动池中的颗粒的通过,并且在基于检测的预定延迟时间之后,照射闪光灯以拾取 由于散射光而产生静止的帧图像,从而进行颗粒的分类和计数。 从采样喷嘴排出的液体样品中的异物被过滤器捕获,并且在从采样喷嘴排出液体样品完成之后被鞘液液体溢出到过滤器的上部。 流通池中的气泡通过排气口去除,并且一旦保留在排气室中,鞘液中的气泡就被除去。 因此,可以防止液体样品/皮肤液体中的异物/气泡进入流动池单元,使得可以高精度地进行测试而不降低测试效率。