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1. 发明申请

US20090030194A1 Process for Production of Glucuronic Acid and/or Glucuronolactone 审中-公开
标题翻译：葡萄糖醛酸和/或葡糖醛酸内酯的制备方法
公开(公告)号：US20090030194A1
公开(公告)日：2009-01-29
申请号：US11913999
申请日：2006-03-30
申请人： Kenichi Hamayasu , Hiroki Tadokoro , Eriko Kishino , Tetsuya Ito , Koki Fujita , Kozo Hara
发明人： Kenichi Hamayasu , Hiroki Tadokoro , Eriko Kishino , Tetsuya Ito , Koki Fujita , Kozo Hara
IPC分类号： C07H1/06
CPC分类号： C12P19/02 , C12P7/62 , C12P17/04
摘要： Provided is a process, by which glucuronic acid and/or glucuronolactone can be produced in high yield, at low cost, in an easy manner and in safe. Also provided is a process for production of glucuronic acid and/or glucuronolactone which is characterized in that sucrose is oxidized to give sucrose carboxylic acid (and a salt thereof) (glucuronyl-fructoside, β-D-fructosyl-(2→1)-α-D-glucuronic acid and a salt thereof), yeast is added so as to hydrolyze fructose residue of the sucrose carboxylic acid and to assimilate the resulting product, and thus produced glucuronic acid and/or glucuronolactone are/is collected.
摘要翻译：提供了一种以低成本，易于且安全地以高产率生产葡糖醛酸和/或葡糖醛酸内酯的方法。还提供了用于生产葡糖醛酸和/或葡糖醛酸内酯的方法，其特征在于将蔗糖氧化得到蔗糖羧酸（及其盐）（葡糖醛酸 - 果糖苷，β-D-果糖基 - （2-> 1） -α-D-葡糖醛酸及其盐），加入酵母以水解蔗糖羧酸的果糖残基并使所得产物同化，从而收集葡萄糖醛酸和/或葡糖醛酸内酯。

2. 发明授权

US5763598A Branched cyclodextrins and method for producing them 失效
标题翻译：支链环糊精及其制备方法
公开(公告)号：US5763598A
公开(公告)日：1998-06-09
申请号：US876979
申请日：1997-06-16
申请人： Kenichi Hamayasu , Tetsuya Ito , Koki Fujita , Kozo Hara , Kyoko Koizumi , Toshiko Tanimoto , Hirofumi Nakano , Sumio Kitahata
发明人： Kenichi Hamayasu , Tetsuya Ito , Koki Fujita , Kozo Hara , Kyoko Koizumi , Toshiko Tanimoto , Hirofumi Nakano , Sumio Kitahata
IPC分类号： C12P19/26 , A61K47/48 , C08B37/16 , A01N43/04 , A61K31/715
CPC分类号： B82Y5/00 , A61K47/48969 , C08B37/0012
摘要： Disclosed are novel branched cyclodextrins having any of an N-acetylglucosamine group, a glucosaminyl group, an N-acetylgalactosaminyl group and a galactosaminyl group as bonded to the hydroxyl group of the cyclodextrin ring by .beta.-bonding therebetween, and their salts. The novel branched cyclodextrins are produced by reacting a solution containing a cyclodextrin and an N-acetylglucosamine or N-acetylgalactosamine with an N-acetylhexosaminidase, and optionally deacetylating the resulting product.
摘要翻译：公开了通过β键连接在环糊精环的羟基上的N-乙酰葡糖胺基，葡糖胺基，N-乙酰半乳糖胺基和半乳糖胺基中的任一种的新型支链环糊精及其盐。新型支链环糊精是通过使含有环糊精和N-乙酰葡糖胺或N-乙酰半乳糖胺的溶液与N-乙酰基己糖胺酶反应，并任选地将所得产物脱乙酰化来制备的。

3. 发明申请

US20100075381A1 Method for Producing Glucuronic Acid by Glucuronic Acid Fermentation 有权
标题翻译：通过葡萄糖醛酸发酵生产葡萄糖醛酸的方法
公开(公告)号：US20100075381A1
公开(公告)日：2010-03-25
申请号：US12598456
申请日：2008-04-21
申请人： Tetsuya Ito , Yuki Ario , Eriko Kishino , Koki Fujita
发明人： Tetsuya Ito , Yuki Ario , Eriko Kishino , Koki Fujita
IPC分类号： C12P19/00 , C12N1/21
CPC分类号： C12P19/02 , C12N1/20 , C12P17/04 , C12R1/01
摘要： The object of the present invention is to provide a microorganism having an excellent ability to specifically oxidize the hydroxymethyl group of glucose, and a method of producing glucuronic acid and/or glucuronolactone by using such a microorganism to directly oxidize glucose, and the invention is directed at a microorganism which produces glucuronic acid directly from glucose and a mutant strain which is capable of specifically oxidizing the hydroxymethyl group of glucose and in which a DNA nucleotide sequence corresponding to 16S rRNA has the nucleotide sequence of SEQ ID NO: 1 in the sequence listing, and the invention is also directed at a method of producing glucuronic acid and/or glucuronolactone by specifically oxidizing the hydroxymethyl group of glucose using the mutant strain, and this invention enables D-glucuronic acid and/or D-glucuronolactone to be produced and furnished easily and safely at a high yield and a low cost.
摘要翻译：本发明的目的是提供一种具有优异的葡萄糖羟甲基氧化能力的微生物，以及通过使用这样的微生物直接氧化葡萄糖来生产葡萄糖醛酸和/或葡糖醛酸内酯的方法，本发明是针对在从葡萄糖直接生产葡萄糖醛酸的微生物和能够特异性氧化葡萄糖羟甲基的突变菌株，其中对应于16S rRNA的DNA核苷酸序列具有序列表中SEQ ID NO：1的核苷酸序列本发明还涉及通过使用突变菌株特异性氧化葡萄糖羟甲基来生产葡萄糖醛酸和/或葡糖醛酸内酯的方法，本发明使D-葡萄糖醛酸和/或D-葡糖醛酸内酯能够生产和配制轻松安全地以高产量和低成本。

4. 发明授权

US08530186B2 Method for producing glucuronic acid by glucuronic acid fermentation 有权
标题翻译：葡萄糖醛酸发酵生产葡萄糖醛酸的方法
公开(公告)号：US08530186B2
公开(公告)日：2013-09-10
申请号：US12598456
申请日：2008-04-21
申请人： Tetsuya Ito , Yuki Ario , Eriko Kishino , Koki Fujita
发明人： Tetsuya Ito , Yuki Ario , Eriko Kishino , Koki Fujita
IPC分类号： C12N1/12 , C12N1/20 , C12P1/00 , C12P1/04 , C12P7/00
CPC分类号： C12P19/02 , C12N1/20 , C12P17/04 , C12R1/01
摘要： The object of the present invention is to provide a microorganism having an excellent ability to specifically oxidize the hydroxymethyl group of glucose, and a method of producing glucuronic acid and/or glucuronolactone by using such a microorganism to directly oxidize glucose, and the invention is directed at a microorganism which produces glucuronic acid directly from glucose and a mutant strain which is capable of specifically oxidizing the hydroxymethyl group of glucose and in which a DNA nucleotide sequence corresponding to 16S rRNA has the nucleotide sequence of SEQ ID NO: 1 in the sequence listing, and the invention is also directed at a method of producing glucuronic acid and/or glucuronolactone by specifically oxidizing the hydroxymethyl group of glucose using the mutant strain, and this invention enables D-glucuronic acid and/or D-glucuronolactone to be produced and furnished easily and safely at a high yield and a low cost.
摘要翻译：本发明的目的是提供一种具有优异的葡萄糖羟甲基氧化能力的微生物，以及通过使用这样的微生物直接氧化葡萄糖来生产葡萄糖醛酸和/或葡糖醛酸内酯的方法，本发明是针对在从葡萄糖直接生产葡萄糖醛酸的微生物和能够特异性氧化葡萄糖羟甲基的突变菌株，其中对应于16S rRNA的DNA核苷酸序列具有序列表中SEQ ID NO：1的核苷酸序列本发明还涉及通过使用突变菌株特异性氧化葡萄糖羟甲基来生产葡萄糖醛酸和/或葡糖醛酸内酯的方法，本发明使D-葡萄糖醛酸和/或D-葡糖醛酸内酯能够生产和配制轻松安全地以高产量和低成本。

5. 发明授权

US06284510B1 &bgr;-fructofuranosidase gene 有权
标题翻译： β-呋喃果糖苷酶基因
公开(公告)号：US06284510B1
公开(公告)日：2001-09-04
申请号：US09503172
申请日：2000-02-14
申请人： Tetsuya Ito , Koki Fujita , Kozo Hara , Takashi Tonozuka , Yoshiyuki Sakano
发明人： Tetsuya Ito , Koki Fujita , Kozo Hara , Takashi Tonozuka , Yoshiyuki Sakano
IPC分类号： C12P2106
CPC分类号： C12N9/2408 , C12N9/2431 , C12Y302/01026
摘要： A &bgr;-fructofuranosidase gene coding for a protein having an amino acid sequence described in Seq. I.D. No. 1 in the Sequence Listing. The gene enables production of &bgr;-fructofuranosidase on a large scale more easily and less dependently on the productivity of microorganisms than the production by cultivation of the microorganisms. Also, the &bgr;-fructofuranosidase gene is useful for the development of variant enzymes that have increased heat resistance and transfer ratio by means of genetic engineering techniques.
摘要翻译：编码具有Seq。所述的氨基酸序列的蛋白质的β-呋喃果糖苷酶基因。 ID。序列表第1号。该基因能够比通过微生物培养的生产更容易且更不依赖于微生物的生产力而大量生产β-呋喃果糖苷酶。此外，β-呋喃果糖苷酶基因可用于通过遗传工程技术开发具有增加的耐热性和转移比的变体酶。

6. 发明申请

US20080202503A1 Crystalline Lactosucrose or Syrup Containing Crystalline Lactosucrose and Use Thereof 审中-公开
标题翻译：结晶乳糖蔗糖或含有结晶乳糖蔗糖的糖浆及其用途
公开(公告)号：US20080202503A1
公开(公告)日：2008-08-28
申请号：US11572717
申请日：2005-06-06
申请人： Kazufumi Murakami , Tetsuya Ito , Koki Fujita , Kozo Hara , Yoshiyuki Sakano , Shigehiro Kamitori
发明人： Kazufumi Murakami , Tetsuya Ito , Koki Fujita , Kozo Hara , Yoshiyuki Sakano , Shigehiro Kamitori
IPC分类号： C13F1/02 , C13K5/00
CPC分类号： A23K50/60 , A23F5/243 , A23G1/40 , A23G3/42 , A23G4/10 , A23K20/163 , A23K50/30 , A23K50/75 , A23L5/00 , A23L7/00 , A23L23/00 , A23L27/33 , A23L29/30 , A23L33/125
摘要： The present invention establishes a crystalline lactosucrose and provides a process for producing the crystalline lactosucrose or a syrup containing crystalline lactosucrose. Further, the present invention has its object to provide a composition, for example, a food or drink, a cosmetic, a drug or the like containing the crystalline lactosucrose.That is, the present invention provides the crystalline lactosucrose or the syrup containing crystalline lactosucrose, the process for producing the crystalline lactosucrose or the syrup containing crystalline lactosucrose, characterized by crystallizing a crystalline lactosucrose from a solution containing lactosucrose and collecting the crystalline lactosucrose, and the use thereof.
摘要翻译：本发明建立了结晶乳糖蔗糖并提供了生产结晶乳糖蔗糖或含有结晶乳糖的糖浆的方法。此外，本发明的目的是提供一种组合物，例如含有结晶乳糖的食品或饮料，化妆品，药物等。也就是说，本发明提供结晶乳糖蔗糖或含有结晶乳糖蔗糖的糖浆，制备结晶乳糖蔗糖的方法或含有结晶乳糖蔗糖的糖浆，其特征在于，从含有乳糖蔗糖的溶液中结晶结晶乳糖并收集结晶乳糖，使用它。

7. 发明申请

US20060234980A1 Antiinflammatory agent, agent for preventing/ameliorating allergic diseases and functional food 审中-公开
标题翻译：抗炎剂，预防/改善过敏性疾病和功能性食物的药剂
公开(公告)号：US20060234980A1
公开(公告)日：2006-10-19
申请号：US10516234
申请日：2003-05-29
申请人： Hiroyuki Hashimoto , Kei Sonoyama , Koki Fujita , Kozo Hara , Masamichi Okada , Shigeharu Mori
发明人： Hiroyuki Hashimoto , Kei Sonoyama , Koki Fujita , Kozo Hara , Masamichi Okada , Shigeharu Mori
IPC分类号： A61K31/716 , A61K31/715 , A61K47/00
CPC分类号： C07H3/06 , A23L33/10 , A61K31/702 , A61K31/715
摘要： It is intended to provide an anti-inflammatory agent made from a highly safe material which can be daily taken as a food, an agent for preventing or ameliorating allergic diseases and a functional food having the above-mentioned effects. The anti-inflammatory agent contains an α-bond galactooligosaccharide as the active ingredient. The agent for preventing or ameliorating allergic diseases contains an α-bond galactooligosaccharide as the active ingredient. The functional food contains an α-bond galactooligosaccharide as the active ingredient. As such α-bond galactooligosaccharide, use can be made of a product synthesized by the dehydration condensation reaction by α-galactosidase with the use of galactose or galactose-containing substance as a substrate.
摘要翻译：本发明提供一种由日常服用的高度安全的材料制成的抗炎剂，用于预防或改善过敏性疾病的药剂和具有上述效果的功能性食品。抗炎剂含有α-键低聚半乳糖作为活性成分。用于预防或改善过敏性疾病的药物含有α-键低聚半乳糖作为活性成分。功能性食品含有α-键低聚半乳糖作为活性成分。作为这样的α-键低聚半乳糖，可以使用通过使用半乳糖或含半乳糖的物质作为底物的α-半乳糖苷酶通过脱水缩合反应合成的产物。

8. 发明授权

US07491518B2 Method of elevating yield of oligosaccharides containing α-galactosyl and anti-candida compositions 失效
标题翻译：提高含有α-半乳糖基和抗念珠菌组合物的寡糖产率的方法
公开(公告)号：US07491518B2
公开(公告)日：2009-02-17
申请号：US10362483
申请日：2001-08-08
申请人： Masamichi Okada , Shigeharu Mori , Hiroyuki Hashimoto , Koki Fujita , Kozo Hara
发明人： Masamichi Okada , Shigeharu Mori , Hiroyuki Hashimoto , Koki Fujita , Kozo Hara
IPC分类号： C12P19/14 , C12P19/12 , C12P19/18
CPC分类号： C12N9/2465 , C12P19/14 , C12Y302/01022
摘要： A novel method of very efficiently elevating the yield of oligosacchrides containing α-galactosyl, compared with the conventional methods, which comprises treating galactose or a galactose-containing material with a specific α-galactosidase and thus performing a dehydrocondensation reaction at a high substrate concentration. Anti-candida compositions originating in foods, having a high safety and excellent anti-candida effect and not being restricted in the supply, which contain as the active ingredient oligosacchrides obtained by treating galactose or a galactose-containing material with an α-galactosidase and thus performing a dehydrocondensation.
摘要翻译：与常规方法相比，非常有效地提高含有α-半乳糖苷的寡糖的产率的新方法，其包括用特定的α-半乳糖苷酶处理半乳糖或含半乳糖的材料，从而以高底物浓度进行脱氢反应。起始于食物的抗念珠菌组合物，具有高安全性和优异的抗念珠菌作用，不受供体限制，其含有通过用α-半乳糖苷酶处理半乳糖或含半乳糖的物质获得的活性成分的寡糖，因此进行脱氢。

9. 发明授权

US5089401A Method for the preparation of fructose-containing oligosaccharide 失效
标题翻译：含有含葡萄糖的寡糖的制备方法
公开(公告)号：US5089401A
公开(公告)日：1992-02-18
申请号：US672388
申请日：1991-03-20
申请人： Koki Fujita , Kozo Hara , Hitoshi Hashimoto , Sumio Kitahata
发明人： Koki Fujita , Kozo Hara , Hitoshi Hashimoto , Sumio Kitahata
IPC分类号： C12P19/14 , C07H3/06 , C12N9/10 , C12N9/26 , C12P19/12 , C12P19/18 , C12P19/24 , C12R1/06
CPC分类号： C12N9/2408 , C12P19/12 , C12P19/24 , Y10S435/83
摘要： An enzymatic method for the preparation of a fructose-containing oligosaccharide, in which a .beta.-fructofuranosidase obtained by culturing Arthrobacter sp. K-1 (FERM BP-3192) as an enzyme is reacted on sucrose, raffinose or stachyose as the donor in the presence of an aldose or ketose as the receptor. The enzyme is characterized by:(1) activity on sucrose for the transglycosidation of a fructosyl group to the receptor in the presence of a monosaccharide, sugar alcohol, alkyl alcohol, glycoside or oligosaccharide;(2) activity for the decomposition of sucrose, elrose, neokestose, xylsucrose, raffinose and stachyose with inactivity on a saccharide selected from the group consisting of 1-kestose, nistose, inulobiose and levan biose;(3) optimum pH of 6.5 to 6.8 at 40.degree. C. with stability at a pH of 5.5 to 10;(4) optimum temperature of 55.degree. C. at a pH of 6.5 exhibiting at least 70% of residual activity at 60.degree. C.;(5) susceptibility to inhibition by the ions of silver, mercury, zinc, copper and tin;(6) two molecular weights of 52,000.+-.2,500 and 58,000.+-.2,500; and(7) two isoelectric points of pH 4.3 and pH 4.6.

10. 发明申请

US20090118226A1 INTESTINAL EOSINOPHIL-SUPPRESSING COMPOSITION 审中-公开
标题翻译：内分泌抑素组合物
公开(公告)号：US20090118226A1
公开(公告)日：2009-05-07
申请号：US12302823
申请日：2007-05-21
申请人： Kazunari Ushida , Sanshiro Saito , Toshiro Sato , Yosuke Isobe , Koki Fujita , Tetsuya Ito
发明人： Kazunari Ushida , Sanshiro Saito , Toshiro Sato , Yosuke Isobe , Koki Fujita , Tetsuya Ito
IPC分类号： A61K31/7004 , A61K31/702 , A61K31/715
CPC分类号： A23K20/163 , A23L33/21 , A61K31/7016 , A61K31/702
摘要： To promote the growth of human beings or animals, prevent the onset of diarrhea and prevent or ameliorate various diseases, it is intended to provide a substance which has an effect of decreasing eosinophils in the intestinal tract and a feed comprising the same as a feed additive. Namely, an intestinal eosinophil-suppressing composition which contains an ▪-linked galactooligosaccharide as the active ingredient. When added in an amount of from 0.01 to 10% by weight, in terms of the saccharide, to an animal feed, this composition is useful in providing an intestinal eosinophil-suppressing feed.
摘要翻译：为了促进人类或动物的生长，预防腹泻的发生并预防或改善各种疾病，旨在提供一种具有减少肠道嗜酸性粒细胞的作用的物质和包含与饲料添加剂相同的饲料。即，含有β-连接的低聚半乳糖作为活性成分的肠嗜酸性粒细胞抑制组合物。当添加量为0.01〜10重量％，就糖而言，与动物饲料相比，该组合物可用于提供肠嗜酸性粒细胞抑制饲料。

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