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1. 发明授权

US08841097B2 Process for producing unsaturated fatty acid-containing oils 有权
标题翻译：用于生产不饱和脂肪酸的油的方法
公开(公告)号：US08841097B2
公开(公告)日：2014-09-23
申请号：US11407984
申请日：2006-04-21
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
IPC分类号： C12P7/64 , A61K31/20 , C07C53/00 , A23L1/30 , A23D9/00 , A23K1/16 , A23L1/29
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3β-ol content.
摘要翻译：通过在含有源自大豆的氮源的培养基中培养微生物而获得的食用油属于被孢霉属（Mortierella subgenus Mortidalla）属。获得的油具有低的24,25-亚甲基胆甾-5-烯-3β-醇含量。

2. 发明申请

US20060252736A1 Process for producing unsaturated fatty acid-containing oils 有权
公开(公告)号：US20060252736A1
公开(公告)日：2006-11-09
申请号：US11482901
申请日：2006-07-10
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
IPC分类号： C12P7/64 , A61K31/56
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3β-ol content.

3. 发明授权

US06746857B2 Media for culturing microorganisms and process for producing unsaturated fatty acids or lipids containing the same 有权
标题翻译：用于培养微生物的培养基和用于生产不饱和脂肪酸或含有它们的脂质的方法
公开(公告)号：US06746857B2
公开(公告)日：2004-06-08
申请号：US09331759
申请日：1999-06-25
申请人： Kenichi Higashiyama , Toshiaki Yaguchi , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Toshiaki Yaguchi , Kengo Akimoto , Sakayu Shimizu
IPC分类号： C12P764
CPC分类号： C12P7/6427 , C12N1/14 , C12P7/6472
摘要： A process for controlling the mycelial morphology of a microorganism belonging to the genus Mortierella during culturing and a process for producing unsaturated fatty acids and a lipid containing them by using a culture medium for culturing a microorganism in which phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the culture medium are in the range of 5 to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively, characterized in that the microorganism belonging to the genus Mortierella is cultured in a culture medium containing phosphate ions in the range of 5 to 60 mM, potassium ions in the range of 5 to 60 mM, sodium ions in the range of 2 to 50 mM, magnesium ions in the range of 0.5 to 9 mM, and calcium ions in the range of 0.5 to 12 mM, respectively, to produce unsaturated fatty acids and the lipid containing them, and the culture medium for culturing a microorganism having phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the range of 5 to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively.
摘要翻译：用于在培养期间控制属于被孢霉属属的微生物的菌丝体形态的方法以及通过使用培养基培养不饱和脂肪酸和含有它们的脂质的方法来培养微生物的方法，其中磷酸根离子，钾离子，钠离子，培养基中的镁离子和钙离子分别为5〜60mM，5〜60mM，2〜50mM，0.5〜9mM，以及0.5〜12mM的范围，其特征在于，属于将被孢霉属属菌体培养在含有5-60mM磷酸根离子，5-60mM范围钾离子，2-50mM范围内的钠离子，0.5- 至9mM，钙离子分别为0.5-12mM，以产生不饱和脂肪酸和含有它们的脂质，以及用于培养具有磷酸根离子，钾离子，钠离子，镁离子的微生物的培养基 s和钙离子分别在5至60mM，5至60mM，2至50mM，0.5至9mM和0.5至12mM的范围内。

4. 发明授权

US6117905A Edible oil containing arachidonic acid and foods containing the same 有权
标题翻译：含有花生四烯酸的食用油和含有它的食品
公开(公告)号：US6117905A
公开(公告)日：2000-09-12
申请号：US284205
申请日：1999-06-29
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu , Nobushige Doisaki , Kiyomi Furihata
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu , Nobushige Doisaki , Kiyomi Furihata
IPC分类号： A23C9/152 , A23D9/00 , A23D9/007 , A23L1/30 , C12P7/64 , C12R1/645 , A61K31/20
CPC分类号： A23D9/00 , A23L33/12 , A23L33/40 , A23V2002/00
摘要： Edible oil containing arachidonic acid obtained from microorganisms belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid are provided. The oil contain little unsaponifiable matters and, above all, the smallest possible amount of sterol with cyclopropane structure which have not been recognized as food components, and are suitable for the production of foods, in particular, infant formula.Arachidonic acid-containing edible oil originating in microorganisms containing not more than 0.8% by weight, preferably not more than 0.6% by weight of unsaponifiable matters and 20% by weight or more of arachidonic acid. Further, these edible oil contain not more than 0.3% by weight, preferably not more than 0.15% by weight of 24,25-methylenecholest-5-en-3 .beta.-ol. The microorganisms are those belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid. These microorganisms belong to the species alpine of the genus Mortierella. Foods including the arachidonic acid-containing edible oil. Formula for premature infants, formula for infants, foods for infants, and foods for pregnant women and nursing mothers, including the arachidonic acid-containing edible oil.
摘要翻译： PCT No.PCT / JP97 / 03631 Sec。 371日期1999年6月29日第 102（e）1999年6月29日PCT PCT 1997年10月9日PCT公布。公开号WO98 / 16119 日期：1998年04月23日提供了从属于被孢霉属的被孢霉属的属于微生物的微生物获得的能够产生花生四烯酸的花生四烯酸的可食用油。该油含有少量不皂化物质，最重要的是尽可能少量的具有环丙烷结构的固醇未被认定为食品成分，并且适用于生产食品，特别是婴儿配方奶粉。来源于不超过0.8重量％，优选不超过0.6重量％的不皂化物和20重量％以上花生四烯酸的微生物的含花生四烯酸的食用油。此外，这些食用油含有不多于0.3重量％，优选不超过0.15重量％的24,25-亚甲基胆甾-5-烯-3β-醇。这些微生物属于被孢霉属的被孢霉属亚属属，并且能够产生花生四烯酸。这些微生物属于被孢霉属的高山属。食物包括含花生四烯酸的食用油。婴幼儿配方奶粉，婴儿配方食品，孕妇食品，哺乳母亲，包括含花生四烯酸的食用油。

5. 发明授权

US07091244B1 Process for preparing fat or oil containing unsaturated fatty acid 有权
标题翻译：制备含不饱和脂肪酸的油脂的方法
公开(公告)号：US07091244B1
公开(公告)日：2006-08-15
申请号：US09254152
申请日：1997-08-27
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
IPC分类号： A61K31/20 , A61K31/22 , C12P7/62 , C12P7/64 , C07C53/00
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： A process for production of unsaturated fatty acid-containing oils which is characterized by culturing a microorganism belonging to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean, and collecting the unsaturated fatty acid-containing oil from the cultured product. The oils are obtained with a low 24,25-methylenecholest-5-en-3β-ol content.
摘要翻译：一种生产含不饱和脂肪酸的油的方法，其特征在于在含有源自大豆的氮源的培养基中培养属于被孢霉属的被孢霉属的被孢霉属的微生物，并从培养产物中收集不饱和脂肪酸的油。获得的油具有低的24,25-亚甲基胆甾-5-烯-3β-醇含量。

6. 发明申请

US20050266538A1 Process for producing arachidonic acid-containing lipids and dihomo-gamma-linolenic acid containing lipids 失效
标题翻译：含有花生四烯酸的脂质和含有dihomo-γ-亚麻酸的脂质的制备方法
公开(公告)号：US20050266538A1
公开(公告)日：2005-12-01
申请号：US11167224
申请日：2005-06-28
申请人： Kengo Akimoto , Kenichi Higashiyama , Sakayu Shimizu
发明人： Kengo Akimoto , Kenichi Higashiyama , Sakayu Shimizu
IPC分类号： C12P7/64 , C12R1/645 , C12N1/16
CPC分类号： C12P7/6472 , Y10S435/911
摘要： A process for producing lipids containing arachidonic acid comprising culturing a microorganism in which ω3 desaturase activity has been decreased or is lacking at a temperature lower than the optimum growth temperature from the start of culturing or after culturing at the optimum growth temperature, said microorganism being obtained by the mutation treatment of a microorganism capable of producing arachidonic acid and belonging to the genus Mortierella and the like; and then recovering lipids containing arachidonic acid from the culture.
摘要翻译：一种含花生四烯酸的脂质的制造方法，其特征在于，在培养开始时或培养后，在最适生长温度下培养微生物，其中ω3去饱和酶活性降低或不足于最佳生长温度的温度，所述微生物获得通过突变处理能够产生花生四烯酸并属于被孢霉属等的微生物; 然后从培养物中回收含有花生四烯酸的脂质。

7. 发明授权

US09493798B2 Process for producing unsaturated fatty acid-containing oils 有权
标题翻译：用于生产不饱和脂肪酸的油的方法
公开(公告)号：US09493798B2
公开(公告)日：2016-11-15
申请号：US11482901
申请日：2006-07-10
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
IPC分类号： A61K47/00 , A23K1/17 , A61K31/20 , A23L1/30 , C12P7/64 , A23D9/00 , A23L1/29
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3β-ol content.
摘要翻译：通过在含有源自大豆的氮源的培养基中培养微生物而获得的食用油属于被孢霉属（Mortierella subgenus Mortidalla）属。获得的油具有低的24,25-亚甲基胆甾-5-烯-3β-醇含量。

8. 发明授权

US09464305B2 Process for producing unsaturated fatty acid-containing oils 有权
公开(公告)号：US09464305B2
公开(公告)日：2016-10-11
申请号：US11287369
申请日：2005-11-28
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
IPC分类号： A61K31/20 , A61K31/22 , A61K47/00 , A23L1/30 , C12P7/64 , A23D9/00 , A23L1/29
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3β-ol content.

9. 发明授权

US07585651B2 Process for producing arachidonic acid-containing lipids 失效
标题翻译：制备含花生四烯酸的脂质的方法
公开(公告)号：US07585651B2
公开(公告)日：2009-09-08
申请号：US11167224
申请日：2005-06-28
申请人： Kengo Akimoto , Kenichi Higashiyama , Sakayu Shimizu
发明人： Kengo Akimoto , Kenichi Higashiyama , Sakayu Shimizu
IPC分类号： C12P7/64
CPC分类号： C12P7/6472 , Y10S435/911
摘要： A process for producing lipids containing arachidonic acid comprising culturing a microorganism in which ω3 desaturase activity has been decreased or is lacking at a temperature lower than the optimum growth temperature from the start of culturing or after culturing at the optimum growth temperature, said microorganism being obtained by the mutation treatment of a microorganism capable of producing arachidonic acid and belonging to the genus Mortierella and the like; and then recovering lipids containing arachidonic acid from the culture.
摘要翻译：一种含花生四烯酸的脂质的制造方法，其特征在于，在培养开始时或培养后，在最适生长温度下培养微生物，其中ω3去饱和酶活性降低或不足于最佳生长温度的温度，所述微生物获得通过突变处理能够产生花生四烯酸并属于被孢霉属等的微生物; 然后从培养物中回收含有花生四烯酸的脂质。

10. 发明申请

US20060189588A1 Process for producing unsaturated fatty acid-containing oils 有权
公开(公告)号：US20060189588A1
公开(公告)日：2006-08-24
申请号：US11407984
申请日：2006-04-21
申请人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
发明人： Kenichi Higashiyama , Kengo Akimoto , Sakayu Shimizu
IPC分类号： A61K31/56 , C12P7/64 , C12N1/21 , A61K31/202
CPC分类号： C12P7/6472 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/40 , A23V2002/00 , C12P7/64 , Y10S426/801 , Y10S426/807 , A23V2250/54252 , A23V2250/1874 , A23V2250/1868 , A23V2250/1862 , A23V2250/187 , A23V2250/612 , A23V2250/28 , A23V2250/70 , A23V2250/156
摘要： An edible oil obtained by culturing a microorganism belong to the genus Mortierella subgenus Mortierella in a medium containing a nitrogen source derived from soybean is discussed. The oils obtained have a low 24,25-methylenecholest-5-en-3β-ol content.

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