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1. 发明授权

US08883994B2 Switchable nucleic acid aptamer probe and uses thereof in tumor living cell detection and in vivo detection 有权
标题翻译：可切换的核酸适体探针及其在肿瘤活细胞检测和体内检测中的应用
公开(公告)号：US08883994B2
公开(公告)日：2014-11-11
申请号：US13639843
申请日：2011-03-22
申请人： Kemin Wang , Hui Shi , Xiaoxiao He , Xiaosheng Ye , Xu Wu , Qiuping Guo , Bing Zhou
发明人： Kemin Wang , Hui Shi , Xiaoxiao He , Xiaosheng Ye , Xu Wu , Qiuping Guo , Bing Zhou
IPC分类号： C07H21/02 , C12Q1/68 , C12P19/34 , C07H21/04 , C07H21/00 , G01N21/64
CPC分类号： A61K48/0058 , A61K49/0054 , C12Q1/6818 , C12Q1/6886 , G01N21/6428 , G01N21/6456 , G01N2021/6432 , C12Q2525/301 , C12Q2525/205
摘要： A switch mode nucleic acid aptamer probe includes a probe main body, a fluorescence generating unit and a fluorescence quenching unit which are respectively connected to two ends of the probe main body. The probe main body includes a nucleic acid aptamer fragment with a function of specifically recognizing target tumor cell and a nucleic acid fragment linked to the nucleic acid aptamer fragment by a connection fragment with a length of 7˜15 nm so as to form a hairpin structure. The ability of competitive hybridization of the nucleic acid fragment with the nucleic acid aptamer fragment is weaker than that of the target tumor cell. The use of the probe of the invention can be at least one of specific detection of tumor living cell in buffer solution, effective detection of tumor living cell in serum, and real-time fluorescence imaging and intravital detection of tumor in living body.
摘要翻译：开关模式核酸适配体探针包括分别连接到探针主体的两端的探针主体，荧光发生单元和荧光淬灭单元。探针主体包括具有特异性识别靶肿瘤细胞的功能的核酸适体片段和通过长度为7〜15nm的连接片段与核酸适体片段连接的核酸片段，以形成发夹结构。核酸片段与核酸适体片段竞争性杂交的能力弱于目标肿瘤细胞。使用本发明的探针可以是缓冲溶液中肿瘤活细胞的特异性检测中的至少一种，血清中有效检测肿瘤活细胞，实时荧光成像和活体内肿瘤的活体检测。

2. 发明申请

US20130251638A1 Switchable Nucleic Acid Aptamer Probe and Uses Thereof in Tumor Living Cell Detection and in Vivo Detection 有权
标题翻译：可切换的核酸适体探针及其在肿瘤生物细胞检测和体内检测中的应用
公开(公告)号：US20130251638A1
公开(公告)日：2013-09-26
申请号：US13639843
申请日：2011-03-22
申请人： Kemin Wang , Hui Shi , Xiaoxiao He , Xiaosheng Ye , Xu Wu , Qiuping Guo , Bing Zhou
发明人： Kemin Wang , Hui Shi , Xiaoxiao He , Xiaosheng Ye , Xu Wu , Qiuping Guo , Bing Zhou
IPC分类号： A61K49/00 , C12Q1/68
CPC分类号： A61K48/0058 , A61K49/0054 , C12Q1/6818 , C12Q1/6886 , G01N21/6428 , G01N21/6456 , G01N2021/6432 , C12Q2525/301 , C12Q2525/205
摘要： The present invention relates to nucleic acid-based detection field, in particularly to a switchable nucleic acid aptamer probe and use thereof. The switchable nucleic acid aptamer probe includes a probe main body as well as a fluorescence generating unit and a fluorescence quenching unit which are respectively connected to two ends of the probe main body. The probe main body includes a nucleic acid aptamer fragment with a function of specifically recognizing target tumor cell and a nucleic acid fragment partially complemented with the nucleic acid aptamer fragment. The nucleic acid aptamer fragment and the nucleic acid fragment are linked by a connection fragment with a length of 7˜15 nm so as to form a hairpin structure. The ability of competitive hybridization of the nucleic acid fragment with the nucleic acid aptamer fragment is weaker than that of the target tumor cell. The use of the probe of the invention can be at least one of specific detection of tumor living cell in buffer solution, effective detection of tumor living cell in serum, and real-time fluorescence imaging and in vivo detection of tumor in living body. The probe of the invention holds relatively high stability, specificity and sensitivity, and its use is easy to operation, fast, sensitive, specific and of low cost.
摘要翻译：本发明涉及基于核酸的检测领域，特别涉及可切换的核酸适体探针及其用途。可切换核酸适体探针包括分别连接到探针主体的两端的探针主体以及荧光发生单元和荧光淬灭单元。探针主体包括具有特异性识别靶肿瘤细胞的功能的核酸适体片段和与核酸适体片段部分补充的核酸片段。核酸适体片段和核酸片段通过长度为7〜15nm的连接片段连接，形成发夹结构。核酸片段与核酸适体片段竞争性杂交的能力弱于目标肿瘤细胞。使用本发明的探针可以是缓冲溶液中肿瘤活细胞的特异性检测，血清中肿瘤活细胞的有效检测以及生物体内肿瘤的实时荧光成像和体内检测中的至少一种。本发明的探针具有较高的稳定性，特异性和灵敏度，其使用易于操作，快速，灵敏，特异性和低成本。

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