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1. 发明授权

US06833258B2 Process for producing transglutaminase 有权
标题翻译：转谷氨酰胺酶的制备方法
公开(公告)号：US06833258B2
公开(公告)日：2004-12-21
申请号：US09892864
申请日：2001-06-28
申请人： Keiichi Yokoyama , Kunio Ono , Daisuke Ejima
发明人： Keiichi Yokoyama , Kunio Ono , Daisuke Ejima
IPC分类号： C12N910
CPC分类号： C12N9/1044 , C12Y203/02013
摘要： The present invention relates to a high-yield process for producing high-activity, high-purity transglutaminase by refolding denatured microorganism-derived transglutaminase by reacting denatured transglutaminase in an aqueous medium under acidic conditions and reconstituting a higher-order enzymatically active state in an aqueous medium at a neutral pH.
摘要翻译：本发明涉及一种生产高活性，高纯度转谷氨酰胺酶的高产率方法，其通过在酸性条件下将变性转谷氨酰胺酶在水性介质中反应并重新折叠变性微生物转谷氨酰胺酶，并在水性中重新构建高级酶活性状态培养基处于中性pH值。

2. 发明授权

US06756482B1 Purified human activin and process for producing the same 有权
标题翻译：纯化的人类激活素及其制备方法
公开(公告)号：US06756482B1
公开(公告)日：2004-06-29
申请号：US09721750
申请日：2000-11-27
申请人： Kunio Ono , Shigekatsu Tsuchiya , Daisuke Ejima , Yuzuru Eto
发明人： Kunio Ono , Shigekatsu Tsuchiya , Daisuke Ejima , Yuzuru Eto
IPC分类号： A61K3824
CPC分类号： C07K14/575 , A61K38/00
摘要： The present invention provides a process for purifying human activin by cation exchange chromatography and chaotropic ion concentration gradient elution.
摘要翻译：本发明提供了通过阳离子交换色谱法和离液序列离子浓度梯度洗脱纯化人激活素的方法。

3. 发明授权

US6084076A Method of refolding human activin A 有权
标题翻译：人激活素A重折叠的方法
公开(公告)号：US6084076A
公开(公告)日：2000-07-04
申请号：US91265
申请日：1999-02-08
申请人： Daisuke Ejima , Kunio Ono , Michiro Sasaki , Yuzuru Eto , Shigekatsu Tsuchiya
发明人： Daisuke Ejima , Kunio Ono , Michiro Sasaki , Yuzuru Eto , Shigekatsu Tsuchiya
IPC分类号： C07K14/575 , C12P21/04 , C07K14/475 , A61K38/18 , C07K1/06 , C12N15/09
CPC分类号： C07K14/575
摘要： A method for industrially producing human activin A which comprises refolding a modified human activin A produced by a microorganism into natural-form human activin A having a biological activity. This method comprises the steps of: (a) solubilizing the modified human activin A with a modifier and then protecting the thiol groups with glutathione and/or sodium sulfite; and (b) dialyzing the protected modified human activin A with a refolding buffer, containing taurodexoycholic acid or its salts and then adding a thiol compound thereto conduct a disulfide bond interchange reaction.
摘要翻译： PCT No.PCT / JP96 / 03700 Sec。 371日期1999年2月8日 102（e）1999年2月8日PCT 1996年12月19日PCT PCT。公开号WO97 / 23638 PCT 日期1997年7月3日工业生产人激活素A的方法，其包括将由微生物产生的经修饰的人激活素A重折叠成具有生物活性的天然形式的人激活素A. 该方法包括以下步骤：（a）用修饰剂溶解修饰的人激活素A，然后用谷胱甘肽和/或亚硫酸钠保护硫醇基团; 和（b）用含有牛磺去氧胆酸或其盐的重折叠缓冲液透析受保护的修饰的人激活素A，然后加入硫醇化合物进行二硫键交换反应。

4. 发明授权

US4044394A Thin film magnetic head with a center tap 失效
标题翻译：薄膜磁头与中心水龙头
公开(公告)号：US4044394A
公开(公告)日：1977-08-23
申请号：US673523
申请日：1976-04-05
申请人： Masanobu Hanazono , Osamu Asai , Kunio Ono
发明人： Masanobu Hanazono , Osamu Asai , Kunio Ono
IPC分类号： G11B5/17 , G11B5/31 , G11B5/20 , G11B5/28
CPC分类号： G11B5/313 , G11B5/3163
摘要： A narrow track width magnetic head can be formed using an integrated circuit formation techniques such as evaporation plating, chemical etching, etc. But, there has been such a problem that it is difficult to provide a center tap in a conventional thin film magnetic head structure including coils of four or more turns. A magnetic head according to the present invention comprising a coil divided into two sections by the center tap, the electrostatic capacitance of each section being substantially the same. The coil may be formed into a precise pattern with ease by photoetching technique. A plurality of sets of conductive films insulated from one another are provided crossing a magnetic core, each set consisting of two conductive films of the same pattern. The alternating layers of the conductive films are connected on the backward of a magnetic gap to provide the two sections of the coil. A center tap is derived from a connection portion of the two sections of the coil and a coil terminal is provided at each of the opposite free ends of the coil. Thus, the thin film magnetic head having a multi-turn coil with a center tap is provided.
摘要翻译：可以使用诸如蒸发镀，化学蚀刻等的集成电路形成技术来形成窄轨道宽度的磁头。但是，存在这样的问题，即难以在传统的薄膜磁头结构中提供中心抽头包括四圈或更多匝的线圈。根据本发明的磁头包括通过中心抽头分成两部分的线圈，每个部分的静电电容基本上相同。线圈可以通过光刻技术容易地形成精确的图案。跨越磁芯设置多个彼此绝缘的导电膜组，每组由相同图案的两个导电膜组成。导电膜的交替层在磁隙的后方连接以提供线圈的两个部分。中心抽头来自线圈的两个部分的连接部分，线圈端子设置在线圈的相对的自由端中的每一个处。因此，提供具有中心抽头的多匝线圈的薄膜磁头。

5. 发明授权

US4961166A Dynamic RAM having a full size dummy cell 失效
标题翻译：具有全尺寸虚拟单元的动态RAM
公开(公告)号：US4961166A
公开(公告)日：1990-10-02
申请号：US729859
申请日：1985-05-02
申请人： Katsuyuki Sato , Kazumasa Yanagisawa , Kunio Ono
发明人： Katsuyuki Sato , Kazumasa Yanagisawa , Kunio Ono
IPC分类号： G11C11/4096 , G11C11/4099
CPC分类号： G11C11/4096 , G11C11/4099
摘要： A dynamic RAM, in which the difference between a data signal level from one of a pair of complementary data lines coupled to a memory cell and a reference potential level of the other of the complementary data lines is differentially amplified by a sense amplifier. The data line taking the reference potential level is coupled to the other data line through a switch element so that its data line capacitance is increased. As a result, the reference potential level is held at a relatively stable level irrespective of a leakage current such as that caused by .alpha. particles. This construction makes it possible to use a full-size dummy cell because the capacitance of the data lines which takes the reference potential level is increased. The reference potential level achieved by the use of the full-size dummy cell is made relatively accurate because of the relative accuracy between the capacitances of the memory cells and the capacitance of the full-size dummy cell.
摘要翻译：动态RAM，其中来自耦合到存储单元的一对互补数据线中的一个的数据信号电平与另一个互补数据线的参考电位电平之间的差异由读出放大器差分放大。采用参考电位电平的数据线通过开关元件耦合到另一条数据线，使其数据线电容增加。结果，不管诸如由α粒子引起的漏电流如何，基准电位电平保持在相对稳定的水平。这种结构使得可以使用全尺寸虚拟单元，因为增加了参考电位电平的数据线的电容。由于存储单元的电容与全尺寸虚拟单元的电容之间的相对精度相对较高，因此通过使用全尺寸虚拟单元实现的参考电位水平相对精确。

6. 发明授权

US4888299A Routing among field effect transistors 失效
标题翻译：场效应晶体管之间的路由
公开(公告)号：US4888299A
公开(公告)日：1989-12-19
申请号：US313627
申请日：1989-02-21
申请人： Yoichi Shiraishi , Junya Sakemi , Kunio Ono , Ichiro Naka
发明人： Yoichi Shiraishi , Junya Sakemi , Kunio Ono , Ichiro Naka
IPC分类号： H01L21/3205 , G06F17/50 , H01L21/82 , H01L23/52 , H01L23/528 , H01L27/118
CPC分类号： H01L23/528 , G06F17/5077 , H01L2924/0002
摘要： Interconnections interconnecting terminals to be connected by routings are dissolved into two-terminal interconnections and it is determined to which kind of terminals the terminals of each two-terminal interconnection belong, among connected diffusion layer, separated diffusion layer and gate. The interconnections are classified into groups by the combination of the kind of two terminals for each interconnection. The respective groups of interconnections are routed in the channel on the field effect transistor row according to a predetermined order of groups.
摘要翻译：通过路由将要连接的互连终端的互连解耦到双端互连，并确定连接的扩散层，分离的扩散层和门之间的每个两端互连的端子属于哪种类型的端子。通过每个互连的两个端子的种类的组合将互连分为组。相应的互连组根据组的预定顺序在场效应晶体管行的通道中布线。

7. 发明授权

US4074280A Thermal recording apparatus 失效
标题翻译：热记录仪
公开(公告)号：US4074280A
公开(公告)日：1978-02-14
申请号：US740680
申请日：1976-11-10
申请人： Yasuyuki Kojima , Kiyohiko Tanno , Tetsuo Doi , Kunio Ono
发明人： Yasuyuki Kojima , Kiyohiko Tanno , Tetsuo Doi , Kunio Ono
IPC分类号： B41J2/355 , G01D15/10 , H04N1/032
CPC分类号： G01D15/10
摘要： A thermal recording apparatus for recording an information signal on the heat-sensitive recording medium in a form of visible information comprises a plurality of heat-generating resistance elements, a drive circuit for supplying selectively drive current to said heat-generating resistance elements in response to the information signal, a recording medium disposed in contact with the plurality of heat-generating resistance elements and adapted to change color upon sensing heat, means for driving the plurality of heat-generating resistance elements and the recording medium relative to each other, and a switching circuit means for inverting the direction of the drive current applied to the heat-generating resistance elements in a predetermined manner.

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