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1. 发明申请

US20110236905A1 METHOD FOR SCREENING CATTLE, CATTLE SCREENED, AND CATTLE SCREENING KIT 审中-公开
标题翻译：用于筛选CATTLE，CATTLE SCREENED和CATTLE SCREENING KIT的方法
公开(公告)号：US20110236905A1
公开(公告)日：2011-09-29
申请号：US12936569
申请日：2009-03-04
申请人： Kazuya Matsumoto , Kouichi Morimoto , Haruka Ikegami , Kouhei Nagai
发明人： Kazuya Matsumoto , Kouichi Morimoto , Haruka Ikegami , Kouhei Nagai
IPC分类号： G01N33/566 , C12Q1/02
CPC分类号： C07K16/18 , G01N33/68 , G01N2333/4718
摘要： There are provided a method for screening individual cattle having useful economic traits in which proteins related to the economic traits of beef cattle are identified by proteomics and are used as biomarkers, and a cattle screening kit for use in the method.The method for screening individual cattle according to the present invention includes the steps of (1) harvesting body tissue from the cattle, (2) extracting the full complement of proteins from the body tissue harvested, (3) detecting a wild-type annexin A5 protein, an isoform of the wild-type annexin A5 protein, or a modified protein of the wild-type annexin A5 protein or the isoform thereof in the full complement of proteins extracted, and (4) determining whether or not the cattle will have a high average carcass weight on the basis of the type of annexin A5 detected. The wild-type annexin A5 protein, the isoform thereof, and the modified protein thereof are detected by two-dimensional electrophoresis or an antigen-antibody reaction.
摘要翻译：提供了一种筛选具有有用的经济性状的个体牛的方法，其中通过蛋白质组学鉴定与牛的经济性状相关的蛋白质并用作生物标志物，以及用于该方法的牛筛选试剂盒。根据本发明的用于筛选个体牛的方法包括以下步骤：（1）从牛收获身体组织，（2）从收获的身体组织中提取全部蛋白质，（3）检测野生型膜联蛋白A5 蛋白质，野生型膜联蛋白A5蛋白的同种型，或野生型膜联蛋白A5蛋白的修饰蛋白或其同源物在提取的全部蛋白质中，以及（4）确定牛是否具有基于检测到膜联蛋白A5的类型的高平均胴体重。通过二维电泳或抗原 - 抗体反应检测野生型膜联蛋白A5蛋白及其同种型及其修饰蛋白。

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