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1. 发明授权

US6153419A Method for quantitative determination of 1,5-anhydroglucitol 失效
标题翻译： 1,5-脱水葡萄糖醇定量测定方法
公开(公告)号：US6153419A
公开(公告)日：2000-11-28
申请号：US930709
申请日：1997-10-16
申请人： Kazuo Aisaka , Sakae Tazoe , Katsuhiko Ando , Keiko Ochiai
发明人： Kazuo Aisaka , Sakae Tazoe , Katsuhiko Ando , Keiko Ochiai
IPC分类号： C12N9/24 , C12Q1/34 , C12Q1/48
CPC分类号： C12Q1/34 , C12N9/2402 , C12Q1/48 , C12Y302/01028 , G01N2333/355 , G01N2333/924 , Y10S435/872
摘要： The present invention relates to a method for quantitative determination of 1,5-anhydroglucitol in a sample, which comprises mixing the sample and an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, and measuring the activity of the enzyme; and a reagent for quantitative determination of 1,5-anhydroglucitol which comprises an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, a substrate for the enzyme, and a reagent for quantitative determination of a product formed by the enzyme activity. The present invention also relates to novel trehalase having a Ki value of 0.33 mM or less for 1,5-anhydroglucitol; and a process for producing novel trehalase having the above-mentioned physicochemical properties, which comprises culturing in a medium a microorganism belonging to the genus Nocardia and capable of producing the trehalase, and recovering the trehalase from the culture.
摘要翻译： PCT No.PCT / JP97 / 00440 Sec。 371日期：1997年10月16日 102（e）1997年10月16日PCT PCT 1997年2月19日PCT公布。公开号WO97 / 31103 日期：1997年8月28日本发明涉及一种样品中1,5-脱水葡萄糖醇的定量测定方法，该方法包括以浓度依赖性方式将样品与具有1,5-脱水葡萄糖醇抑制活性的酶，并测定酶的活性; 以及用于定量测定1,5-脱水葡萄糖醇的试剂，该试剂含有以浓度依赖性方式被1,5-脱水葡萄糖醇抑制的活性的酶，酶底物和定量产物形成试剂通过酶活性。本发明还涉及1,5-脱水葡萄糖醇的Ki值为0.33mM以下的新型海藻糖酶; 以及具有上述物理化学性质的新型海藻糖酶的制造方法，其包括在培养基中培养属于诺卡氏菌属的微生物并能够生产海藻糖酶，并从培养物中回收海藻糖酶的方法。

2. 发明授权

US06448029B1 Method and reagent for quantitative determination of 1,5-anhydroglucitol 有权
标题翻译：用于定量测定1,5-脱水葡萄糖醇的方法和试剂
公开(公告)号：US06448029B1
公开(公告)日：2002-09-10
申请号：US09929038
申请日：2001-08-15
申请人： Sakae Tazoe , Akira Miike
发明人： Sakae Tazoe , Akira Miike
IPC分类号： C12Q132
CPC分类号： C12Q1/32 , C12Q1/54 , Y10S435/966 , Y10S435/975
摘要： The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.
摘要翻译：本发明提供了用于确定特定成分的简单方法，例如，含有葡萄糖的样品中的1,5-脱水葡萄糖醇（1,5-AG），以及可用于该方法的试剂和试剂盒。在一个实施方案中，提供了用于测定1,5-AG的方法，其包括将样品与将葡萄糖转化为果糖-1,6-二磷酸并将1,5-AG转化为1,5-AG的酶系统接触 -6-磷酸形成1,5-AG-6-磷酸，通过1,5-AG-6-磷酸脱氢酶的作用在氧化型辅酶存在下使样品中的1,5-AG-6-磷酸脱氢，并测定由脱氢反应形成的还原型辅酶的量。还提供了一种在该方法中有用的试剂和试剂盒。

3. 发明授权

US06309852B1 Method and reagent for quantitative determination of 1,5-anhydroglucitol 失效
标题翻译：用于定量测定1,5-脱水葡萄糖醇的方法和试剂
公开(公告)号：US06309852B1
公开(公告)日：2001-10-30
申请号：US09456998
申请日：1999-12-07
申请人： Sakae Tazoe , Akira Miike
发明人： Sakae Tazoe , Akira Miike
IPC分类号： C12Q132
CPC分类号： C12Q1/32 , C12Q1/54 , Y10S435/966 , Y10S435/975
摘要： The present invention provides a simple method for the determination of a specific component, e.g. 1,5-anhydroglucitol (1,5-AG) in a sample containing glucose, and a reagent and a reagent kit useful in the method. In one embodiment, a method for the determination of 1,5-AG is provided which comprises contacting the sample with an enzyme system which converts glucose into fructose-1,6-diphosphate and converts 1,5-AG into 1,5-AG-6-phosphate to form 1,5-AG-6-phosphate, dehydrogenating 1,5-AG-6-phosphate in the sample by the action of 1,5-AG-6-phosphate dehydrogenase in the presence of an oxidized coenzyme, and measuring the amount of the reduced coenzyme formed by the dehydrogenation reaction. A reagent and a reagent kit useful in this method are also provided.
摘要翻译：本发明提供了用于确定特定成分的简单方法，例如，含有葡萄糖的样品中的1,5-脱水葡萄糖醇（1,5-AG），以及可用于该方法的试剂和试剂盒。在一个实施方案中，提供了用于测定1,5-AG的方法，其包括将样品与将葡萄糖转化为果糖-1,6-二磷酸并将1,5-AG转化为1,5-AG的酶系统接触 -6-磷酸形成1,5-AG-6-磷酸，通过1,5-AG-6-磷酸脱氢酶的作用在氧化型辅酶存在下使样品中的1,5-AG-6-磷酸脱氢，并测定由脱氢反应形成的还原型辅酶的量。还提供了一种在该方法中有用的试剂和试剂盒。

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