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1. 发明申请

US20060008869A1 Process for production of biopterin compound 失效
标题翻译：生物蝶呤化合物的生产工艺
公开(公告)号：US20060008869A1
公开(公告)日：2006-01-12
申请号：US11194564
申请日：2005-08-02
申请人： Masayuki Yabuta , Kazuaki Furukawa , Nobue Miyamoto , Katsuhiko Yamamoto , Kazuhiro Ohsuye
发明人： Masayuki Yabuta , Kazuaki Furukawa , Nobue Miyamoto , Katsuhiko Yamamoto , Kazuhiro Ohsuye
IPC分类号： C12P25/00 , C07D475/02
CPC分类号： C12N15/52 , C12N9/00 , C12P17/182
摘要： The present invention provides a transformed cell which is transformed by at least one gene of enzymes participating in biosynthesis of tetrahydrobiopterin and a process for the production of a biopterin compound using the same. In accordance with the present invention, the biopterin compound can be produced in large quantities in an industrial advantageous manner from less expensive materials.
摘要翻译：本发明提供了通过参与四氢生物喋呤的生物合成的酶的至少一个基因转化的转化细胞和使用其生产生物蝶呤化合物的方法。根据本发明，生物蝶呤化合物可以以较便宜的材料以工业上有利的方式大量生产。

2. 发明授权

US5834249A Process for production of protein 失效
标题翻译：蛋白质生产工艺
公开(公告)号：US5834249A
公开(公告)日：1998-11-10
申请号：US759945
申请日：1996-12-03
申请人： Kazuaki Furukawa , Keijiro Sugimura , Kazuhiro Ohsuye
发明人： Kazuaki Furukawa , Keijiro Sugimura , Kazuhiro Ohsuye
IPC分类号： C07K14/57 , C12N1/38 , C12N9/02 , C12P21/02 , C12R1/91 , C07K14/54 , C12N5/06
CPC分类号： C12N9/0071 , C07K14/57 , C12N1/38 , C12P21/02
摘要： A process for production of a desired protein comprising the steps of: a culturing animal cells capable of producing the desired protein in a medium containing trichostatin compounds; and recovering the desired protein from the culture.
摘要翻译：一种制备所需蛋白质的方法，包括以下步骤：培养能够在含有曲古抑菌素化合物的培养基中产生所需蛋白质的动物细胞; 并从培养物中回收所需的蛋白质。

3. 发明授权

US07807421B2 Process for production of biopterin compound 失效
标题翻译：生物蝶呤化合物的生产工艺
公开(公告)号：US07807421B2
公开(公告)日：2010-10-05
申请号：US11194564
申请日：2005-08-02
申请人： Masayuki Yabuta , Kazuaki Furukawa , Nobue Miyamoto , Katsuhiko Yamamoto , Kazuhiro Ohsuye
发明人： Masayuki Yabuta , Kazuaki Furukawa , Nobue Miyamoto , Katsuhiko Yamamoto , Kazuhiro Ohsuye
IPC分类号： C12P17/16 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/00 , C12N9/04 , C12N9/88 , C12N9/78 , C12P21/00 , C12Q1/34 , C07K14/00 , C07H21/00
CPC分类号： C12N15/52 , C12N9/00 , C12P17/182
摘要： The present invention provides a transformed cell which is transformed by at least one gene of enzymes participating in biosynthesis of tetrahydrobiopterin and a process for the production of a biopterin compound using the same. In accordance with the present invention, the biopterin compound can be produced in large quantities in an industrial advantageous manner from less expensive materials.
摘要翻译：本发明提供了通过参与四氢生物喋呤的生物合成的酶的至少一个基因转化的转化细胞和使用其生产生物蝶呤化合物的方法。根据本发明，生物蝶呤化合物可以以较便宜的材料以工业上有利的方式大量生产。

4. 发明授权

US5976833A Method for animal cell culture 失效
标题翻译：动物细胞培养方法
公开(公告)号：US5976833A
公开(公告)日：1999-11-02
申请号：US716533
申请日：1996-09-19
申请人： Kazuaki Furukawa , Kazuhiro Ohsuye
发明人： Kazuaki Furukawa , Kazuhiro Ohsuye
IPC分类号： C12N5/00 , C12N5/10 , C12N9/02 , C12N15/09 , C12P21/02 , C12R1/91 , C12N15/00
CPC分类号： C12N9/0071 , C12N5/00 , C12P21/02 , C12N2523/00
摘要： The object of the present invention is to provide a method for improving productivity in the production of useful substances by animal cells. The present invention discloses a method for animal cell culture to produce a desired substance, comprising the steps of (1) culturing animal cells at a temperature at which the animal cells can grow; and (2) culturing the animal cells at a lower temperature.
摘要翻译：本发明的目的是提供一种提高动物细胞生产有用物质生产率的方法。本发明公开了一种用于产生所需物质的动物细胞培养方法，包括以下步骤：（1）在动物细胞能够生长的温度下培养动物细胞; 和（2）在较低温度培养动物细胞。

5. 发明授权

US06262232B1 Recombinant C-terminal &agr;-amidating enzyme 失效
公开(公告)号：US06262232B1
公开(公告)日：2001-07-17
申请号：US07219375
申请日：1988-07-15
申请人： Kazuhiro Ohsuye , Katsuhiko Kitano , Shoji Tanaka , Hisayuki Matsuo , Kensaku Mizuno
发明人： Kazuhiro Ohsuye , Katsuhiko Kitano , Shoji Tanaka , Hisayuki Matsuo , Kensaku Mizuno
IPC分类号： C07K1446
CPC分类号： C12N9/0071 , C12Y114/17003
摘要： A C-terminal &agr;-amidating enzyme of Xenopus laevis and precursor thereof produced by a recombinant DNA technique; a DNA coding for the enzyme or precursor thereof; a plasmid containing the DNA; a host organism transformed with the plasmid; a process for production of the enzyme using the transformant; and a process for production of a C-terminal &agr;-amidated peptide using the enzyme.

6. 发明授权

US6037145A Process for production of protein 失效
标题翻译：蛋白质生产工艺
公开(公告)号：US6037145A
公开(公告)日：2000-03-14
申请号：US523373
申请日：1995-09-05
申请人： Masayuki Yabuta , Kazuhiro Ohsuye
发明人： Masayuki Yabuta , Kazuhiro Ohsuye
IPC分类号： C12N1/21 , C12N9/52 , C12N15/62 , C12N15/63
CPC分类号： C12N9/52 , C12N15/62 , C07K2319/00
摘要： A process for the production of a desired polypeptide comprising the steps of: (1) transforming host cells with an expression vector comprising a gene coding for a fusion protein comprising a desired polypeptide and a protective polypeptide; (2) culturing the transformed host cells so as to express said gene to produce a fusion protein; and (3) excising the desired polypeptide from the fusion protein with a protease intrinsic to the host cells. According to the present invention, a large amount of a desired polypeptide can be produced at a low cost. Especially according to the present invention, a large amount of S. aureus V8 protease can be efficiently produced at low cost using a safe host such as E. coli according to gene recombination procedures.
摘要翻译：一种制备所需多肽的方法，包括以下步骤：（1）用包含编码包含所需多肽和保护性多肽的融合蛋白的基因的表达载体转化宿主细胞; （2）培养转化的宿主细胞，以表达所述基因以产生融合蛋白; 和（3）用融合蛋白切割所需的多肽与宿主细胞固有的蛋白酶。根据本发明，可以低成本生产大量所需的多肽。特别是根据本发明，可以使用安全宿主如大肠杆菌根据基因重组方法以低成本有效地生产大量的金黄色葡萄球菌V8蛋白酶。

7. 发明授权

US5955307A Plasmid vector and use thereof for the production of interferon 失效
标题翻译：质粒载体及其用于生产干扰素的用途
公开(公告)号：US5955307A
公开(公告)日：1999-09-21
申请号：US915851
申请日：1997-08-21
申请人： Kazuhiro Ohsuye , Shoji Tanaka
发明人： Kazuhiro Ohsuye , Shoji Tanaka
IPC分类号： C12N15/09 , C07H21/04 , C07K14/57 , C12N1/20 , C12N1/21 , C12N15/00 , C12N15/70 , C12P21/00 , C12P21/02 , C12R1/19 , C12N15/23 , C12P21/06
CPC分类号： C12N15/70 , C07K14/57
摘要： Escherichia coli plasmid vectors are provided which have a 5'-terminal untranslated region (inclusive of the promoter region and Shine-Dalgarno sequence) of the Escherichia coli lipoprotein gene, which region is improved to thereby enable direct production of useful polypeptides in substantially complete form.
摘要翻译：提供大肠杆菌质粒载体，其具有大肠杆菌脂蛋白基因的5'末端非翻译区（包括启动子区和Shine-Dalgarno序列），该区被改进，从而能够以基本上完整的形式直接生产有用的多肽。

8. 发明授权

US5747321A Mutant Staphylococcus aureus V8 proteases 失效
标题翻译：突变型金黄色葡萄球菌V8蛋白酶
公开(公告)号：US5747321A
公开(公告)日：1998-05-05
申请号：US657192
申请日：1996-06-03
申请人： Masayuki Yabuta , Kazuhiro Ohsuye
发明人： Masayuki Yabuta , Kazuhiro Ohsuye
IPC分类号： C07K14/58 , C12N9/52 , C07H21/04 , C12N1/20 , C12N15/00
CPC分类号： C07K14/58 , C12N9/52 , C07K2319/00
摘要： Mutant proteases are obtained with one or more mutation sites in the natural V8 protease protein, and with enzyme activities even in the presence of high urea concentrations. Inactivation of enzyme activity is minimized even in the presence of high concentrations of urea, to thus allow lower amounts of enzyme to be added to urea-containing reaction systems and shorten reaction times. As an additional advantage, the ability to cleave proteins in the presence of high urea concentrations makes it possible to obtain hitherto unobtainable peptide fragments.
摘要翻译：用天然V8蛋白酶蛋白质中的一个或多个突变位点获得突变蛋白酶，即使在高尿素浓度的存在下也可获得酶活性。即使在高浓度的尿素存在下，酶活性的失活最小化，从而允许较少量的酶加入到含脲反应体系中并缩短反应时间。作为另外的优点，在高尿素浓度存在下切割蛋白质的能力使得可能获得迄今无法获得的肽片段。

9. 发明授权

US06602681B1 Enzymatic production of a C-terminal &agr;-amidated peptide or protein 失效
标题翻译： C末端α-酰胺化肽或蛋白质的酶促产生
公开(公告)号：US06602681B1
公开(公告)日：2003-08-05
申请号：US08845381
申请日：1997-04-25
申请人： Kazuhiro Ohsuye , Katsuhiko Kitano , Shoji Tanaka , Hisayuki Matsuo , Kensaku Mizuno
发明人： Kazuhiro Ohsuye , Katsuhiko Kitano , Shoji Tanaka , Hisayuki Matsuo , Kensaku Mizuno
IPC分类号： C12P1206
CPC分类号： C12N9/0071 , C12Y114/17003
摘要： A C-terminal &agr;-amidating enzyme of Xenopus laevis and precursor thereof produced by a recombinant DNA technique; a DNA coding for the enzyme or precursor thereof; a plasmid containing the DNA; a host organism transformed with the plasmid; a process for production of the enzyme using the transformant; and a process for production of a C-terminal &agr;-amidated peptide using the enzyme.
摘要翻译：通过重组DNA技术产生非洲爪蟾的C-末端α-酰胺化酶及其前体; 编码酶或其前体的DNA; 含有DNA的质粒; 用质粒转化的宿主生物; 使用转化体生产酶的方法; 以及使用该酶制备C末端α-酰胺化肽的方法。

10. 发明授权

US07344856B1 Method of controlling cleavage by OmpT protease 失效
标题翻译：通过OmpT蛋白酶控制切割的方法
公开(公告)号：US07344856B1
公开(公告)日：2008-03-18
申请号：US09674777
申请日：2000-03-03
申请人： Kazuaki Okuno , Masayuki Yabuta , Kazuhiro Ohsuye
发明人： Kazuaki Okuno , Masayuki Yabuta , Kazuhiro Ohsuye
IPC分类号： C12P21/06 , C12P21/00 , C12N9/48 , C12N1/20 , C12N15/00
CPC分类号： C07K14/585 , C07K14/58 , C07K14/605 , C07K2319/50 , C07K2319/75 , C12N15/62 , C12N15/63
摘要： Methods of using OmpT protease are provided. In particular, the invention provides for a method of controlling cleavage of a polypeptide by OmpT protease comprising, converting a sequence site comprising two arbitrary consecutive amino acids and/or amino acid(s) in the vicinity of the site in the polypeptide into other amino acids; which method comprises setting lysine or arginine as the amino acid at the −1-position concerning the site and setting a specific amino acid as the amino acid at the +1-position; and/or setting specific amino acid(s) as the amino acid(s) at the −4-position and/or the −6-position relative to the site; so that a desired part of the polypeptide is cleaved by OmpT protease and/or an undesired part of the polypeptide is not (or hardly) cleaved by OmpT protease. A method of producing a target polypeptide is also provided.
摘要翻译：提供了使用OmpT蛋白酶的方法。特别地，本发明提供了一种控制OmpT蛋白质切割多肽的方法，其包括：将包含多肽中位点附近的两个任意连续氨基酸和/或氨基酸的序列位点转化成其他氨基酸; 该方法包括将赖氨酸或精氨酸设置为与位点相关的-1位的氨基酸，并将特定的氨基酸设置为+ 1位的氨基酸; 和/或将特异性氨基酸设置为相对于位点的-4位和/或-6位的氨基酸; 使得所需部分的多肽被OmpT蛋白酶切割和/或多余的不需要的部分不被OmpT蛋白酶切割（或几乎不被切割）。还提供了产生靶多肽的方法。

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