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1. 发明授权

US4613573A Automatic bacterial colony transfer apparatus 失效
标题翻译：自动菌落转运装置
公开(公告)号：US4613573A
公开(公告)日：1986-09-23
申请号：US495489
申请日：1983-05-17
申请人： Katsujiro Shibayama , Fukuo Iwaya , Kensaku Takahashi , Masumi Nukumi , Tatuhito Tuji , Michio Okuma , Yoji Odawara
发明人： Katsujiro Shibayama , Fukuo Iwaya , Kensaku Takahashi , Masumi Nukumi , Tatuhito Tuji , Michio Okuma , Yoji Odawara
IPC分类号： C12M1/32 , C12M1/36 , C12M1/00 , C12M1/12 , C12M1/26
CPC分类号： C12M33/02 , Y10S435/808
摘要： An apparatus for automatically transferring a desired one of bacterial colonies grown in a medium in a culture Petri dish onto a medium in a test Petri dish or a test tube by using a bacterial colony pick-up/transfer element of the disposable type or a bacterial colony pick-up/transfer element of the reuse type and by using various mechanisms controlled by a computer.
摘要翻译：一种用于通过使用一次性类型的细菌菌落拾取/转移元件或细菌将在培养培养皿中的培养基中生长的期望的一个细菌菌落自动转移到测试培养皿或试管中的培养基上的装置殖民地拾取/转移元件，并使用由计算机控制的各种机制。

2. 发明授权

US5151362A Apparatus containing a septum which impedes cell permeation for cell culture and method of use 失效
标题翻译：含有阻碍细胞培养的细胞渗透的隔膜和使用方法的装置
公开(公告)号：US5151362A
公开(公告)日：1992-09-29
申请号：US505326
申请日：1990-04-05
申请人： Hideo Kawaguchi , Kouji Takeuchi , Tadashi Ishibashi , Norio Shimizu , Yoji Odawara
发明人： Hideo Kawaguchi , Kouji Takeuchi , Tadashi Ishibashi , Norio Shimizu , Yoji Odawara
IPC分类号： C12M3/02 , C12M3/06 , C12N5/02
CPC分类号： C12M29/04 , C12M29/10 , C12M41/00 , Y10S435/813
摘要： By culturing cells in an apparatus containing a first zone in which a cell suspended medium is present and a second zone in which liquid containing a physiological saline solution and/or nutrient sources is present, the first zone being separated from the second zone by a septum with characteristics, such as pores size or electrostatic charges, which impedes the cells from freely permeating through, while allowing at least one of nutrient sources, cell wastes or the product to move between the first zone and the second zone via the septum to proliferate cells in the first zone, wherein a flow rate via the septum is controlled in such a manner that the cell count moved from the first zone into the second zone via the septum does not exceed the cell count increased by cell growth in the first zone. Thus, cell culture at a high cell density can be achieved in a large scale.
摘要翻译：通过在包含存在细胞悬浮介质的第一区域和存在含有生理盐水溶液和/或营养源的液体的第二区域的装置中培养细胞，第一区域通过隔膜与第二区域分离具有阻止细胞自由渗透的特征，例如孔径或静电电荷，同时允许营养源，细胞废物或产物中的至少一种通过隔膜在第一区和第二区之间移动以增殖细胞在第一区域中，其中以通过隔膜从第一区域移动到第二区域的方式控制经由隔膜的流速不超过由第一区域中的细胞生长增加的细胞计数。因此，可以大规模地实现高细胞密度的细胞培养。

3. 发明授权

US4491522A Anaerobic digestion process for organic wastes 失效
标题翻译：有机废物厌氧消化过程
公开(公告)号：US4491522A
公开(公告)日：1985-01-01
申请号：US551730
申请日：1983-11-15
申请人： Masahiko Ishida , Ryoichi Haga , Tadashi Ishibashi , Yoji Odawara
发明人： Masahiko Ishida , Ryoichi Haga , Tadashi Ishibashi , Yoji Odawara
IPC分类号： B09B3/00 , C02F3/28 , C02F3/30 , C02F11/04
CPC分类号： C02F3/28 , C02F3/30 , Y02E50/343 , Y10S435/801
摘要： The present invention relates to an anaerobic digestion process for organic wastes with a high efficiency and an improved recovery rate of methane.This process comprises the following steps: (1) anaerobically digesting organic wastes under simultaneous presence of facultative anaerobic bacteria and obligatory anaerobic bacteria, (2) separating the anaerobically digested slurry obtained from the step (1) into a liquor fraction containing the two groups of bacteria and an undecomposed solid fraction, (3) mixing the liquor fraction obtained from the step (2) with the surplus activated sludge slurry produced by anaerobically treating organic waste water, under anaerobic condition, (4) separating the mixed slurry obtained from the step (3) into a solid fraction containing the surplus activated sludge which has absorbed the two groups of bacteria and a liquor fraction, and recycling back the solid fraction obtained from the step (4) as the sources of the two groups of bacteria and nitrogen to the step (1).
摘要翻译：本发明涉及一种具有高效率和提高甲烷回收率的有机废物的厌氧消化方法。该方法包括以下步骤：（1）在兼性厌氧菌和强制性厌氧菌同时存在下厌氧消化有机废物，（2）将由步骤（1）获得的厌氧消化浆液分离成含有两组细菌和未分解的固体部分，（3）将在步骤（2）中获得的液体馏分与在厌氧条件下厌氧处理有机废水产生的剩余活性污泥浆混合，（4）将从步骤（3）成为含有吸收了两组细菌和液体馏分的剩余活性污泥的固体成分，将从步骤（4）获得的固体成分作为两组细菌和氮源的来源循环回到步骤（1）。

4. 发明授权

US4356268A Microorganism for treating waste water 失效
标题翻译：用于处理废水的微生物
公开(公告)号：US4356268A
公开(公告)日：1982-10-26
申请号：US213222
申请日：1980-12-05
申请人： Norio Shimizu , Yoji Odawara , Yasunori Masaki
发明人： Norio Shimizu , Yoji Odawara , Yasunori Masaki
IPC分类号： C02F3/12 , C02F3/34 , C12N1/20
CPC分类号： C12R1/05 , C02F3/34 , Y10S435/829
摘要： The invention relates to an improvement of the floc-formation property of activated sludge contained in waste water.A waste water treatment process comprises steps culturing a novel strain-Alcaligenes faecalis HRL-1 - and adding the cultured cells to to-be-treated waste water.
摘要翻译：本发明涉及废水中所含活性污泥的絮凝形成性能的改进。废水处理方法包括培养新的粪肠杆菌菌株HRL-1的步骤，并将培养的细胞加入待处理的废水中。

5. 发明授权

US4356092A Method for treating waste water 失效
标题翻译：废水处理方法
公开(公告)号：US4356092A
公开(公告)日：1982-10-26
申请号：US298263
申请日：1981-08-31
申请人： Norio Shimizu , Yoji Odawara , Yasunori Masaki
发明人： Norio Shimizu , Yoji Odawara , Yasunori Masaki
IPC分类号： C02F3/12 , C02F3/34
CPC分类号： C12R1/05 , C02F3/34 , Y10S435/829
摘要： The invention relates to an improvement of the floc-formation property of activated sludge contained in waste water.A waste water treatment process comprises steps culturing a novel strain--Alcaligenes faecalis HRL--1--and adding the cultured cells to to-be-treated waste water.
摘要翻译：本发明涉及废水中所含活性污泥的絮凝形成性能的改进。废水处理方法包括培养新的粪肠产碱杆菌HRL-1的步骤，并将培养的细胞加入待处理的废水中。

6. 发明授权

US4891310A Method of controlling culture 失效
标题翻译：控制文化的方法
公开(公告)号：US4891310A
公开(公告)日：1990-01-02
申请号：US843613
申请日：1986-03-25
申请人： Norio Shimizu , Shinichi Fukuzono , Nobuko Nishimura , Yoji Odawara , Tomoaki Sumitani
发明人： Norio Shimizu , Shinichi Fukuzono , Nobuko Nishimura , Yoji Odawara , Tomoaki Sumitani
IPC分类号： C12M1/36 , C12N1/00 , C12N1/20 , C12N1/21 , C12N9/38 , C12P21/02 , C12R1/19 , C12R1/545
CPC分类号： C12M41/34 , C12N9/2471 , C12P21/02 , C12Y302/01023 , C07K2319/00 , C07K2319/02 , C07K2319/61
摘要： A method of controlling culture wherein cells are aerobically cultured to produce metabolite is characterized by adding substrate and/or inducer to the culture broth, judging the endpoint of the growth of the cells and/or the production of the metabolite or terminating the culture under the guidance of a change in the ratio of the first parameter based on the amount of carbon dioxide evolved by the cell culture to the second parameter based on the amount of oxygen consumed by the cell culture. The ratio is a respiratory quotient, for instance.
摘要翻译：一种控制培养的方法，其中细胞被有氧培养以产生代谢物，其特征在于向培养液中加入底物和/或诱导物，判断细胞生长的终点和/或代谢物的产生或终止培养物基于由细胞培养物消耗的氧气的量，基于由细胞培养物产生的二氧化碳的量与第二参数的第一参数的比率的变化的指导。该比例是呼吸商，例如。

7. 发明授权

US4442006A Anaerobic digestion process 失效
标题翻译：厌氧消化过程
公开(公告)号：US4442006A
公开(公告)日：1984-04-10
申请号：US327227
申请日：1981-12-03
申请人： Masahiko Ishida , Ryooichi Haga , Yoji Odawara
发明人： Masahiko Ishida , Ryooichi Haga , Yoji Odawara
IPC分类号： B09B3/00 , C02F3/28
CPC分类号： C02F3/286 , C12M21/04 , C12M23/58 , C12M45/06 , Y02E50/343
摘要： First, the organic waste slurry of sewage sludge and/or kitchen garbage is stored in a stable condition after effecting partially thereto a liquefaction treatment in advance by adding liquefying bacteria, and next this slurry is effectively digested anaerobically by way of a liquefaction/gasification-mixed step or a liquefaction/gasification separated step.
摘要翻译：首先，通过添加液化细菌，预先将污水污泥和/或厨房垃圾的有机废弃物浆料部分地进行液化处理后稳定地储存，然后通过液化/气化 - 混合步骤或液化/气化分离步骤。

8. 发明授权

US5139938A Production of substances with an acetic acid-producing and assimilating bacterium inhibited by acetic acid 失效
标题翻译：生产含ACE酸的乙酸生产和补充细菌的物质
公开(公告)号：US5139938A
公开(公告)日：1992-08-18
申请号：US157719
申请日：1988-02-19
申请人： Norio Shimizu , Shinichi Fukuzono , Kiyoshi Fujimori , Nobuko Nishimura , Yoji Odawara
发明人： Norio Shimizu , Shinichi Fukuzono , Kiyoshi Fujimori , Nobuko Nishimura , Yoji Odawara
IPC分类号： C12M1/36 , C12N1/00 , C12N1/20 , C12P1/00 , C12P21/02
CPC分类号： C12M41/32 , C12M41/48 , C12P1/00 , C12P21/02 , Y10S435/849
摘要： Cultivation of microorganisms or animal cells or plant cells is carried out to produce high density cultivation, high cell yield and high production of desired metabolite products by monitoring acetate concentration in culture broth and regulating assimilation of acetate in the culture broth by the microorganisms or animal cells or plant cells to control the acetate concentration to a set value or less. Preferably, an acetic acid-producing bacterium that is inhibited by acetic acid and is capable of assimilating acetic acid is cultured to produce biologically active substances such as enzymes. The bacterium may be a recombinant Escherichia coli and an inducer which acts on a promoter in an expression vector is added to produce the desired metabolite product. The inducer is 3-.beta.- indolylacrylic acid when the promoter is trp-promoter or isopropyl-.beta.-D-thiogalactoside when the promoter is lac-promoter or tacpromoter.

9. 发明授权

US4510243A Anaerobic digestion process of wastes containing cellulose 失效
标题翻译：含纤维素废物的厌氧消化过程
公开(公告)号：US4510243A
公开(公告)日：1985-04-09
申请号：US434321
申请日：1982-10-14
申请人： Ryoichi Haga , Masahiko Ishida , Yoji Odawara
发明人： Ryoichi Haga , Masahiko Ishida , Yoji Odawara
IPC分类号： C02F11/04 , B32B15/04 , C12P5/02 , C02F3/30 , C12N1/22
CPC分类号： C12P5/023 , C12M21/04 , C12M45/06 , Y02E50/343 , Y10S435/801 , Y10S435/822
摘要： The present invention relates to an anaerobic digestion process of wastes containing cellulose, in which, after liquefying by bringing wastes containing cellulose in slurry state into contact with facultative anaerobic bacteria with pH adjusted between 6.5-8.0 and oxidation-reduction potential controlled between -50-300 mV with oxygen supply, the liquefied slurry is then converted into methane and carbon dioxide by bringing it into contact with obligatory anaerobic bacteria to recover methane efficiently.
摘要翻译：本发明涉及含有纤维素的废物的厌氧消化过程，其中通过将含有浆料状态的纤维素的废物与兼性厌氧菌接触而液化后，pH调节为6.5-8.0，氧化还原电位控制在-50- 随着氧气供应300 mV，液化浆料通过与强制性厌氧菌接触而转化成甲烷和二氧化碳，以有效回收甲烷。

10. 发明授权

US4213857A Anaerobic digestion process 失效
标题翻译：厌氧消化过程
公开(公告)号：US4213857A
公开(公告)日：1980-07-22
申请号：US942285
申请日：1978-09-14
申请人： Masahiko Ishida , Ryoichi Haga , Yoji Odawara
发明人： Masahiko Ishida , Ryoichi Haga , Yoji Odawara
IPC分类号： C02F3/28 , C02F3/30 , C02F3/34 , C02F11/04 , C12P5/02 , C02C1/16
CPC分类号： C02F3/286 , C02F3/30 , C02F3/34 , C12P5/023 , Y02E50/343 , Y02P20/129
摘要： The present invention relates to a process for digestion treatment of organic wastes that are difficult to digest, with a high efficiency and an improved recovery rate of methane.The wastes, before subjected to anaerobic treatment, are heated at 55.degree.-75.degree. C. within an acid or alkaline solution for dissolving digestible ingredients and the insoluble ingredients are eliminated before being transferred to an anaerobic digestion step. By exercising a pre-treatment for dissolving and separating digestible ingredients, the size of the container will be minimized as a result of the shortened time required for digestion and the recovery rate of methane will be improved.
摘要翻译：本发明涉及一种以高效率和提高的甲烷回收率对难以消化的有机废物进行消化处理的方法。在进行厌氧处理之前，将废物在酸性或碱性溶液中在55-75℃下加热，以溶解可消化的成分，并将不溶性成分除去，然后转移至厌氧消化步骤。通过进行溶解和分离可消化成分的预处理，由于消化所需的时间缩短并且甲烷的回收率将得到改善，容器的尺寸将被最小化。

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