专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20120070912A1 DETECTION METHOD AND QUANTIFICATION METHOD FOR TARGET SUBSTANCE 有权
标题翻译：目标物质的检测方法和量化方法
公开(公告)号：US20120070912A1
公开(公告)日：2012-03-22
申请号：US13322809
申请日：2010-05-21
申请人： Katsuhiko Kitsugi , Satoru Sugita , Noriyuki Ohnishi
发明人： Katsuhiko Kitsugi , Satoru Sugita , Noriyuki Ohnishi
IPC分类号： G01N33/53 , G01N21/76 , G01N21/25
CPC分类号： G01N33/583 , G01N21/78 , G01N33/542 , G01N33/582
摘要： Provided are a detection method and a quantification method for a detection target, which can detect and quantify the detection target rapidly, inexpensively, simply, and with high accuracy in a variety of environments. A method for detecting the detection target in a specimen includes processes in which there are mixed a first binding substance which binds a first material containing a stimuli-responsive polymer and a first affinity substance which has affinity for the detection target, a second binding substance which binds a hydrophilic second material and a second affinity substance which has affinity for the detection target, and a specimen; the resulting mixture is placed under conditions where the stimuli-responsive polymer agglutinates and the mixture is developed into a developing carrier, or the resulting mixture is developed into a developing carrier and the mixture is placed under conditions where the stimuli-responsive polymer agglutinates; the signal produced by the presence of the first binding substance or the second binding substance in the developing carrier is verified; and the presence of the detection target in the specimen is determined when the signal is different from that when the detection target is absent. The first affinity substance and the second affinity substance can bind to different positions of the detection target.
摘要翻译：提供了一种用于检测目标的检测方法和量化方法，其可以在各种环境中快速，廉价，高精度地检测和量化检测目标。用于检测标本中的检测目标的方法包括将与含有刺激响应性聚合物的第一材料和对检测对象具有亲和性的第一亲和物质结合的第一结合物质混合的方法，结合亲水性第二材料和对检测对象具有亲和性的第二亲和物质和试样; 将所得混合物放置在刺激响应性聚合物凝集并将混合物显影成显影载体的条件下，或将所得混合物显影成显影载体，并将该混合物置于刺激响应性聚合物凝集的条件下; 验证由显影载体中的第一结合物质或第二结合物质的存在产生的信号; 当信号与检测对象不存在时的信号不同时，确定样本中检测对象的存在。第一亲和物质和第二亲和物质可以结合到检测对象的不同位置。

2. 发明授权

US09063144B2 Detection method and quantification method for target substance 有权
标题翻译：目标物质的检测方法和定量方法
公开(公告)号：US09063144B2
公开(公告)日：2015-06-23
申请号：US13322809
申请日：2010-05-21
申请人： Katsuhiko Kitsugi , Satoru Sugita , Noriyuki Ohnishi
发明人： Katsuhiko Kitsugi , Satoru Sugita , Noriyuki Ohnishi
IPC分类号： G01N33/543 , G01N33/58 , G01N21/78 , G01N33/542
CPC分类号： G01N33/583 , G01N21/78 , G01N33/542 , G01N33/582
摘要： Provided are a detection method and a quantification method for a detection target, which can detect and quantify the detection target rapidly, inexpensively, simply, and with high accuracy in a variety of environments. A method for detecting the detection target in a specimen includes processes in which there are mixed a first binding substance which binds a first material containing a stimuli-responsive polymer and a first affinity substance which has affinity for the detection target, a second binding substance which binds a hydrophilic second material and a second affinity substance which has affinity for the detection target, and a specimen; the resulting mixture is placed under conditions where the stimuli-responsive polymer agglutinates and the mixture is developed into a developing carrier, or the resulting mixture is developed into a developing carrier and the mixture is placed under conditions where the stimuli-responsive polymer agglutinates; the signal produced by the presence of the first binding substance or the second binding substance in the developing carrier is verified; and the presence of the detection target in the specimen is determined when the signal is different from that when the detection target is absent. The first affinity substance and the second affinity substance can bind to different positions of the detection target.
摘要翻译：提供了一种用于检测目标的检测方法和量化方法，其可以在各种环境中快速，廉价，高精度地检测和量化检测目标。用于检测标本中的检测目标的方法包括将与含有刺激响应性聚合物的第一材料和对检测对象具有亲和性的第一亲和物质结合的第一结合物质混合的方法，结合亲水性第二材料和对检测对象具有亲和性的第二亲和物质和试样; 将所得混合物放置在刺激响应性聚合物凝集并将混合物显影成显影载体的条件下，或将所得混合物显影成显影载体，并将该混合物置于刺激响应性聚合物凝集的条件下; 验证由显影载体中的第一结合物质或第二结合物质的存在产生的信号; 当信号与检测对象不存在时的信号不同时，确定样本中检测对象的存在。第一亲和物质和第二亲和物质可以结合到检测对象的不同位置。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式