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1. 发明授权

US08481316B2 Method for differentiating embryonic stem cells into cells expressing AQP-1 有权
标题翻译：将胚胎干细胞分化成表达AQP-1的细胞的方法
公开(公告)号：US08481316B2
公开(公告)日：2013-07-09
申请号：US12669457
申请日：2008-07-21
申请人： Karl M. Schumacher , Jackie Y. Ying , Annegret Schumacher , Karthikeyan Narayanan , Gunter Maubach
发明人： Karl M. Schumacher , Jackie Y. Ying , Annegret Schumacher , Karthikeyan Narayanan , Gunter Maubach
IPC分类号： C12N5/00 , C12N5/02 , C12N5/071
CPC分类号： C12N5/0686 , A61F2/06 , C12N2501/11 , C12N2501/155 , C12N2506/02
摘要： The present invention relates to methods of differentiating a human embryonic stem (ES) cell into a cell, specifically a renal epithelial cell, expressing AQP-I. The methods disclosed comprise culturing human ES cells in a renal specific medium in the presence of an extracellular matrix molecule. The cells produced according to said method can be used to treat renal related disorders such as renal failure, nephrosis, Bright's disease and glomerulitis.
摘要翻译：本发明涉及将人胚胎干（ES）细胞分化成表达AQP-1的细胞，特别是肾上皮细胞的方法。所公开的方法包括在细胞外基质分子的存在下在肾特异性培养基中培养人ES细胞。根据所述方法产生的细胞可用于治疗肾脏相关疾病如肾衰竭，肾病，光明病和肾小球炎。

2. 发明申请

US20110070280A1 METHOD FOR DIFFERENTIATING EMBRYONIC STEM CELLS INTO CELLS EXPRESSING AQP-1 有权
标题翻译：将胚胎干细胞分化成表达AQP-1的细胞的方法
公开(公告)号：US20110070280A1
公开(公告)日：2011-03-24
申请号：US12669457
申请日：2008-07-21
申请人： Karl M. Schumacher , Jackie Y. Ying , Annegret Schumacher , Karthikeyan Narayanan , Gunter Maubach
发明人： Karl M. Schumacher , Jackie Y. Ying , Annegret Schumacher , Karthikeyan Narayanan , Gunter Maubach
IPC分类号： A61F2/00 , C12N5/0735 , A61K35/12 , A61P13/12
CPC分类号： C12N5/0686 , A61F2/06 , C12N2501/11 , C12N2501/155 , C12N2506/02
摘要： The present invention relates to methods of differentiating a human embryonic stem (ES) cell into a cell, specifically a renal epithelial cell, expressing AQP-I. The methods disclosed comprise culturing human ES cells in a renal specific medium in the presence of an extracellular matrix molecule. The cells produced according to said method can be used to treat renal related disorders such as renal failure, nephrosis, Bright's disease and glomerulitis.
摘要翻译：本发明涉及将人胚胎干（ES）细胞分化成表达AQP-1的细胞，特别是肾上皮细胞的方法。所公开的方法包括在细胞外基质分子的存在下在肾特异性培养基中培养人ES细胞。根据所述方法产生的细胞可用于治疗肾脏相关疾病如肾衰竭，肾病，光明病和肾小球炎。

3. 发明申请

US20100285585A1 METHOD FOR EXTRACELLULAR MATRIX MEDIATED DIFFERENTIATION AND PROLIFERATION OF STEM CELLS 失效
标题翻译：超细胞基质介导的分化和干细胞增殖的方法
公开(公告)号：US20100285585A1
公开(公告)日：2010-11-11
申请号：US12746512
申请日：2008-12-05
申请人： Jackie Y. Ying , Andrew Chwee Aun Wan , Karthikeyan Narayanan , Karl M. Schumacher
发明人： Jackie Y. Ying , Andrew Chwee Aun Wan , Karthikeyan Narayanan , Karl M. Schumacher
IPC分类号： C12N5/0775
CPC分类号： C12N5/0606 , C12N5/0663 , C12N2502/13 , C12N2502/22 , C12N2502/256 , C12N2533/90
摘要： There is provided a method of culturing a stem cell on extracellular matrix extracted from support cells and in a stem cell culture medium comprising medium conditioned by the support cells
摘要翻译：提供了一种培养从支持细胞提取的细胞外基质上的干细胞的方法，以及包含由支持细胞调节的培养基的干细胞培养基

4. 发明授权

US08372643B2 Method for extracellular matrix mediated differentiation and proliferation of stem cells 失效
标题翻译：细胞外基质介导干细胞分化和增殖的方法
公开(公告)号：US08372643B2
公开(公告)日：2013-02-12
申请号：US12746512
申请日：2008-12-05
申请人： Jackie Y. Ying , Andrew Chwee Aun Wan , Karthikeyan Narayanan , Karl M. Schumacher
发明人： Jackie Y. Ying , Andrew Chwee Aun Wan , Karthikeyan Narayanan , Karl M. Schumacher
IPC分类号： C12N5/00 , C12N5/02
CPC分类号： C12N5/0606 , C12N5/0663 , C12N2502/13 , C12N2502/22 , C12N2502/256 , C12N2533/90
摘要： There is provided a method of culturing a stem cell on extracellular matrix extracted from support cells and in a stem cell culture medium comprising medium conditioned by the support cells.
摘要翻译：提供了一种培养从支持细胞提取的细胞外基质上的干细胞的方法，以及包含由支持细胞调节的培养基的干细胞培养基。

5. 发明申请

US20130023024A1 CONJUGATE OF MAGNETIC PARTICLE AND SURFACE MODIFIER LINKED THROUGH CLEAVABLE PEPTIDE BOND 审中-公开
标题翻译：通过可清除的肽结合连接的磁性颗粒和表面改性剂的结合
公开(公告)号：US20130023024A1
公开(公告)日：2013-01-24
申请号：US13637524
申请日：2011-03-31
申请人： Jackie Y. Ying , Karthikeyan Narayanan , Alex Wei Haw Lin , Andrew Chwee Aun Wan , Yuangang Zheng , Nandanan Erathodiyil
发明人： Jackie Y. Ying , Karthikeyan Narayanan , Alex Wei Haw Lin , Andrew Chwee Aun Wan , Yuangang Zheng , Nandanan Erathodiyil
IPC分类号： C12N13/00 , C12N5/071 , C07K7/06 , B82Y99/00
CPC分类号： B82Y5/00 , G01N33/54326 , G01N33/56966
摘要： A conjugate is provided for cell processing, which comprises a magnetic particle and a surface modifier having specific affinity to a target cell. The particle and modifier are linked through a cleavable peptide bond. In a method of cell processing, the conjugate is attached to a target cell; the target cell attached to the conjugate is subject to magnetic processing; the peptide bond is cleaved to separate the processed target cell from the magnetic particle; the target cell separated from the magnetic particle is attached to a substrate. The magnetic particle may include an iron oxide, and the surface modifier may include a glucosamine. The particle and modifier may be linked by a linker comprising a protease recognition site and a peptide bond. The linker links the surface modifier to the particle, and cleavage of the peptide bond is catalyzed by a specific protease that recognizes the protease recognition site.
摘要翻译：提供了用于细胞处理的缀合物，其包含对靶细胞具有特异性亲和力的磁性颗粒和表面改性剂。颗粒和改性剂通过可切割的肽键连接。在细胞处理方法中，缀合物附着于靶细胞; 附着于缀合物的靶细胞进行磁加工; 肽键被切割以将加工的靶细胞与磁性颗粒分离; 从磁性颗粒分离的靶细胞附着于基材。磁性颗粒可以包括氧化铁，并且表面改性剂可以包括葡糖胺。颗粒和改性剂可以通过包含蛋白酶识别位点和肽键的接头连接。接头将表面改性剂连接到颗粒上，肽键的切割由识别蛋白酶识别位点的特异性蛋白酶催化。

6. 发明申请

US20110243851A1 GLUCOSE-PEG CONJUGATES FOR REDUCING GLUCOSE TRANSPORT INTO A CELL 审中-公开
标题翻译：用于减少葡萄糖输送到细胞中的葡萄糖-PEG结合物
公开(公告)号：US20110243851A1
公开(公告)日：2011-10-06
申请号：US13139170
申请日：2009-12-11
申请人： Karthikeyan Narayanan , Andrew Chwee Aun Wan , Jackie Y. Ying , Nandanan Erathodiyil
发明人： Karthikeyan Narayanan , Andrew Chwee Aun Wan , Jackie Y. Ying , Nandanan Erathodiyil
IPC分类号： A61K49/00 , C07H15/12 , C07H15/26 , C12N5/071 , C12N5/09 , C12Q1/02 , A61K31/7008 , A61P35/00
CPC分类号： C08G65/00 , A61K31/08 , A61K31/16 , A61K31/69 , C07C235/08 , C07H15/08 , G01N33/5091
摘要： There is provided a glucose-PEG conjugate comprising a PEG moiety conjugated to a linear glucose moiety at the C1 position of the glucose moiety. The glucose-PEG conjugate may be used to reduce glucose transport into a cell and may be used to treat a proliferative disorder.
摘要翻译：提供了包含在葡萄糖部分的C1位上与线性葡萄糖部分缀合的PEG部分的葡萄糖-PEG缀合物。葡萄糖-PEG轭合物可用于降低葡萄糖转运至细胞中，并可用于治疗增殖性疾病。

7. 发明申请

US20130295637A1 FIBROUS SUBSTRATES FOR CELL PROPAGATION AND DIFFERENTIATION 审中-公开
标题翻译：纤维基质用于细胞传播和分化
公开(公告)号：US20130295637A1
公开(公告)日：2013-11-07
申请号：US13814501
申请日：2011-08-05
申请人： Hongfang Lu , Andrew Chwee Aun Wan , Jackie Y. Ying , Karthikeyan Narayanan
发明人： Hongfang Lu , Andrew Chwee Aun Wan , Jackie Y. Ying , Karthikeyan Narayanan
IPC分类号： C12N11/04
CPC分类号： C12N11/04 , C12N5/0012 , C12N5/0606 , C12N2533/72 , C12N2533/74
摘要： The present invention relates to a method of releasably encapsulating pluripotent embryonic stem cells in a degradable continuous polyionic fiber for tissue culture, wherein the encapsulated embryonic stem cells are able to maintain a pluripotent phenotype in tissue culture; the method comprising (a) contacting an aqueous solution of a polyanion with an aqueous solution of a polycation to form an interface between the aqueous solution of polyanion and the aqueous solution of polycation, and wherein the aqueous solution of polyanion or the aqueous solution of polycation or both the aqueous solution of polyanion and the aqueous solution of polycation comprises a suspension of pluripotent embryonic stem cells; (b) drawing a continuous polyionic fiber which comprises encapsulated pluripotent embryonic stem cells from the interface; (c) passing the continuous polyionic fiber comprising encapsulated pluripotent embryonic stem cells in a continuous process through a solution which reduces secondary complexation of the components of the polyionic fiber.
摘要翻译：本发明涉及一种将多能胚胎干细胞可释放地包封在用于组织培养的可降解连续聚离子纤维中的方法，其中包封的胚胎干细胞能够在组织培养中保持多能表型; 该方法包括（a）将聚阴离子的水溶液与聚阳离子水溶液接触以形成聚阴离子水溶液与聚阳离子水溶液之间的界面，并且其中聚阴离子水溶液或聚阳离子水溶液或聚阴离子水溶液和聚阳离子水溶液两者均包含多能胚胎干细胞的悬浮液; （b）从界面绘制包含多能胚胎干细胞的连续聚离子纤维; （c）使包含多能胚胎干细胞的连续聚离子纤维在连续过程中通过减少聚离子纤维组分的次级络合的溶液。

8. 发明授权

US07868153B2 Cinnamic acid 4-hydroxylase 有权
标题翻译：肉桂酸4-羟化酶
公开(公告)号：US07868153B2
公开(公告)日：2011-01-11
申请号：US11452342
申请日：2006-06-14
申请人： Gefu Wang-Pruski , Sandra Cantle , Karthikeyan Narayanan
发明人： Gefu Wang-Pruski , Sandra Cantle , Karthikeyan Narayanan
IPC分类号： A01H5/00 , A01H5/04 , C12Q1/68 , C12P19/34 , C12N15/29
CPC分类号： C12N15/8243 , C12N9/0073 , C12N15/8279
摘要： After-cooking darkening is a gray-black discoloration of the potato tuber, formed after cooking by the oxidation of an iron-chlorogenic acid complex. Cinnamic acid 4-hydroxylase (C4H) is a key enzyme involved in the biosynthesis of chlorogenic acid. The full-length c4h gene was cloned and sequenced from both genomic DNA and cDNA of Russet Burbank tuber tissue by PCR and 5′ and 3′ RACE. The gene expression levels of c4h were examined by Northern hybridization, relative quantitative RT-PCR and real time quantitative RT-PCR in potato cultivars and wide selection of diploid clones varying in susceptibility to after-cooking darkening. Results suggest that there is a relationship between the levels of c4h gene expression and the degree of after-cooking darkening in potato tubers. The inhibition of C4H gene expression and over expression of C4H expression were also examined. The successful inhibition of the gene expression will lead to the reduced biosynthesis of chlorogenic acid, reducing the susceptibility of after-cooking darkening. The successful overexpression of the C4H gene will lead to the increase in the chlorogenic acid in plant tissues, gaining the resistance to diseases. In addition, due to the natural antioxidant activity of chlorogenic acid, overexpression of C4H gene will lead to its over production in plant tissues, such as potato tubers.
摘要翻译：烹饪后的变黑是通过铁 - 绿原酸络合物的氧化烹饪后形成的马铃薯块茎的灰黑色变色。肉桂酸4-羟化酶（C4H）是涉及绿原酸生物合成的关键酶。通过PCR和5'和3'RACE克隆和测序来自Russet Burbank块茎组织的基因组DNA和cDNA的全长c4h基因。通过Northern杂交，相对定量RT-PCR和马铃薯品种实时定量RT-PCR检测c4h的基因表达水平，并对广泛选择的二倍体克隆进行了广泛的选择，这些变异对烹饪后变暗的敏感性变化。结果表明，c4h基因表达水平与马铃薯块茎后期蒸煮程度之间存在一定的关系。还检查了C4H基因表达的抑制和C4H表达的过表达。基因表达的成功抑制将导致绿原酸的生物合成减少，降低了后烹调变深度的敏感性。 C4H基因的成功过表达将导致植物组织中绿原酸的增加，获得抗病能力。此外，由于绿原酸的天然抗氧化活性，C4H基因的过表达将导致植物组织如马铃薯块茎过度生产。

9. 发明申请

US20070163006A1 Cinnamic acid 4-hydroxylase 有权
标题翻译：肉桂酸4-羟化酶
公开(公告)号：US20070163006A1
公开(公告)日：2007-07-12
申请号：US11452342
申请日：2006-06-14
申请人： Gefu Wang-Pruski , Sandra Cantle , Karthikeyan Narayanan
发明人： Gefu Wang-Pruski , Sandra Cantle , Karthikeyan Narayanan
IPC分类号： A01H5/00 , C07H21/02 , C12N15/82
CPC分类号： C12N15/8243 , C12N9/0073 , C12N15/8279
摘要： After-cooking darkening is a gray-black discoloration of the potato tuber, formed after cooking by the oxidation of an iron-chlorogenic acid complex. Cinnamic acid 4-hydroxylase (C4H) is a key enzyme involved in the biosynthesis of chlorogenic acid. The full-length c4h gene was cloned and sequenced from both genomic DNA and cDNA of Russet Burbank tuber tissue by PCR and 5′ and 3′ RACE. The gene expression levels of c4h were examined by Northern hybridization, relative quantitative RT-PCR and real time quantitative RT-PCR in potato cultivars and wide selection of diploid clones varying in susceptibility to after-cooking darkening. Results suggest that there is a relationship between the levels of c4h gene expression and the degree of after-cooking darkening in potato tubers. The inhibition of C4H gene expression and over expression of C4H expression were also examined. The successful inhibition of the gene expression will lead to the reduced biosynthesis of chlorogenic acid, reducing the susceptibility of after-cooking darkening. The successful overexpression of the C4H gene will lead to the increase in the chlorogenic acid in plant tissues, gaining the resistance to diseases. In addition, due to the natural antioxidant activity of chlorogenic acid, overexpression of C4H gene will lead to its over production in plant tissues, such as potato tubers.
摘要翻译：烹饪后的变黑是通过铁 - 绿原酸络合物的氧化烹饪后形成的马铃薯块茎的灰黑色变色。肉桂酸4-羟化酶（C4H）是涉及绿原酸生物合成的关键酶。通过PCR和5'和3'RACE克隆和测序来自Russet Burbank块茎组织的基因组DNA和cDNA的全长c4h基因。通过Northern杂交，相对定量RT-PCR和马铃薯品种实时定量RT-PCR检测c4h的基因表达水平，并对广泛选择的二倍体克隆进行了广泛的选择，这些变异对烹饪后变暗的敏感性变化。结果表明，c4h基因表达水平与马铃薯块茎后期蒸煮程度之间存在一定的关系。还检查了C4H基因表达的抑制和C4H表达的过表达。基因表达的成功抑制将导致绿原酸的生物合成减少，降低了后烹调变深度的敏感性。 C4H基因的成功过表达将导致植物组织中绿原酸的增加，获得抗病能力。此外，由于绿原酸的天然抗氧化活性，C4H基因的过表达将导致植物组织如马铃薯块茎过度生产。

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