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1. 发明授权

US07790430B2 Cellulase resistant to surfactants 有权
标题翻译：纤维素酶耐表面活性剂
公开(公告)号：US07790430B2
公开(公告)日：2010-09-07
申请号：US12101683
申请日：2008-04-11
申请人： Kaoru Okakura , Koji Yanai
发明人： Kaoru Okakura , Koji Yanai
IPC分类号： C12N9/24 , C12N9/42 , D06M16/00 , D21C1/00 , C12Q1/34 , A23L1/31 , C07H21/04
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulose having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulose NCE5 are substituted. Further, a polynucleotide encoding the novel cellulose, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulose preparation and a washing composition containing the cellulose are disclosed. The cellulose of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素，其中纤维素NCE5的氨基酸序列中第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有纤维素的纤维素制剂和洗涤组合物。本发明的纤维素耐表面活性剂，即使在碱性条件下也能保持高活性。

2. 发明授权

US07374921B2 Cellulase tolerant to surfactants 有权
标题翻译：纤维素酶耐受表面活性剂
公开(公告)号：US07374921B2
公开(公告)日：2008-05-20
申请号：US10533310
申请日：2003-10-31
申请人： Kaoru Okakura , Koji Yanai
发明人： Kaoru Okakura , Koji Yanai
IPC分类号： C12N9/42 , C12N5/16 , C12Q1/34 , C12P21/06 , C07H21/04 , C11D3/00 , C07K1/00
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulase having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulase NCE5 are substituted. Further, a polynucleotide encoding the novel cellulase, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulase preparation and a washing composition containing the cellulase are disclosed. The cellulase of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素酶，其中纤维素酶NCE5的氨基酸序列中的第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素酶的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有该纤维素酶的纤维素酶制剂和洗涤组合物。本发明的纤维素酶对表面活性剂具有抗性，即使在碱性条件下也能保持高活性。

3. 发明申请

US20060035361A1 Novel cellulase tolerant to surfactants 有权
标题翻译：新型纤维素酶耐受表面活性剂
公开(公告)号：US20060035361A1
公开(公告)日：2006-02-16
申请号：US10533310
申请日：2003-10-31
申请人： Kaoru Okakura , Koji Yanai
发明人： Kaoru Okakura , Koji Yanai
IPC分类号： C11D3/386 , C07H21/04 , C12P21/06 , C12N9/24
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulase having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulase NCE5 are substituted. Further, a polynucleotide encoding the novel cellulase, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulase preparation and a washing composition containing the cellulase are disclosed. The cellulase of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素酶，其中纤维素酶NCE5的氨基酸序列中的第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素酶的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有该纤维素酶的纤维素酶制剂和洗涤组合物。本发明的纤维素酶对表面活性剂具有抗性，即使在碱性条件下也能保持高活性。

4. 发明申请

US20080199938A1 Novel Cellulase Resistant to Surfactant 有权
标题翻译：新型纤维素酶耐表面活性剂
公开(公告)号：US20080199938A1
公开(公告)日：2008-08-21
申请号：US12101683
申请日：2008-04-11
申请人： Kaoru OKAKURA , Koji Yanai
发明人： Kaoru OKAKURA , Koji Yanai
IPC分类号： C12N9/42 , C07H21/04 , C12N15/00 , C12N1/00 , D06M16/00 , D21C1/00
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01004 , D21C5/005 , D21H17/22
摘要： Disclosed is a novel cellulose having an amino acid sequence in which the 162nd and/or 166th amino acid residues in the amino acid sequence of cellulose NCE5 are substituted. Further, a polynucleotide encoding the novel cellulose, an expression vector containing the polynucleotide, a host cell transformed with the expression vector, and a cellulose preparation and a washing composition containing the cellulose are disclosed. The cellulose of the present invention is resistant to surfactants, and maintains a high activity even under alkaline conditions.
摘要翻译：公开了具有氨基酸序列的新型纤维素，其中纤维素NCE5的氨基酸序列中第162位和/或第166位氨基酸残基被取代。此外，公开了编码新型纤维素的多核苷酸，含有多核苷酸的表达载体，用表达载体转化的宿主细胞，以及含有纤维素的纤维素制剂和洗涤组合物。本发明的纤维素耐表面活性剂，即使在碱性条件下也能保持高活性。

5. 发明授权

US07285404B1 Cyclic depsipeptide synthetase and method for recombinant production 失效
标题翻译：循环depipipeptide合成酶和重组生产方法
公开(公告)号：US07285404B1
公开(公告)日：2007-10-23
申请号：US10070387
申请日：2000-09-07
申请人： Naoki Midoh , Kaoru Okakura , Koichi Miyamoto , Manabu Watanabe , Koji Yanai , Tetsuya Yasutake , Sato Aihara , Takafumi Futamura , Horst Kleinkauf , Takeshi Murakami
发明人： Naoki Midoh , Kaoru Okakura , Koichi Miyamoto , Manabu Watanabe , Koji Yanai , Tetsuya Yasutake , Sato Aihara , Takafumi Futamura , Horst Kleinkauf , Takeshi Murakami
IPC分类号： C12N9/00
CPC分类号： C12N9/00 , C12P21/00
摘要： The present invention provides an enzyme that synthesizes a cyclic depsipeptide, particularly the substance 1022, and a gene thereof. A cyclic depsipeptide synthetase according to the present invention comprises (a) an amino acid sequence of SEQ ID NO: 2 or (b) a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that have one or more modifications selected from a substitution, a deletion, an addition and an insertion and has cyclic depsipeptide synthetase activity. A cyclic depsipeptide synthetase gene according to the present invention comprises a nucleotide sequence encoding a cyclic depsipeptide synthetase. The present invention also provides a recombinant vector and a transformant for expressing the cyclic depsipeptide synthetase, and a mass production system for the cyclic depsipeptide. The present invention further provides a method for producing the cyclic depsipeptide synthetase.
摘要翻译：本发明提供合成环状缩肽，特别是物质1022及其基因的酶。根据本发明的循环倍肽合成酶包含（a）SEQ ID NO：2的氨基酸序列或（b）SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列，其具有一个或多个修饰选择从取代，缺失，添加和插入，并具有循环衰老肽合成酶活性。根据本发明的环状缩肽酶合成酶基因包含编码环状倍肽合成酶的核苷酸序列。本发明还提供了用于表达环状缩酚酸肽合成酶的重组载体和转化体，以及用于环状缩酚酸肽的批量生产系统。本发明还提供了一种用于制备环状缩酚酸肽合成酶的方法。

6. 发明授权

US07109018B1 Transformant producing secondary metabolite modified with functional group and novel biosynthesis genes 失效
标题翻译：用功能组和新生物合成基因修饰的转化体产生次级代谢产物
公开(公告)号：US07109018B1
公开(公告)日：2006-09-19
申请号：US10089514
申请日：2000-09-29
申请人： Koji Yanai , Kaoru Okakura , Shohei Yasuda , Manabu Watanabe , Koichi Miyamoto , Naoki Midoh , Takeshi Murakami
发明人： Koji Yanai , Kaoru Okakura , Shohei Yasuda , Manabu Watanabe , Koichi Miyamoto , Naoki Midoh , Takeshi Murakami
IPC分类号： C12N1/20 , C12N5/10 , C07H21/04
CPC分类号： C12N9/0004 , C12N9/88 , C12N9/90 , C12N15/52 , C12P13/001 , C12P13/02
摘要： An objective of the present invention is to provide a transformant altered so as to produce a secondary metabolite in which a benzene ring of the secondary product is modified at the para-position with a functional group containing a nitrogen atom. A transformant according to the present invention is a transformant of an organism producing a secondary metabolite having a benzene ring skeleton without substitution at the para-position with a functional group containing a nitrogen atom, said transformant being transformed by introducing genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a gene encoding an amino acid sequence (SEQ ID NO: 2) having 4-amino-4-deoxychorismic acid synthase activity, a gene encoding an amino acid sequence (SEQ ID NO: 4) having 4-amino-4-deoxychorismic acid mutase activity and a gene encoding an amino acid sequence (SEQ ID NO: 6) having 4-amino-4-deoxyprephenic acid dehydrogenase activity, so as to produce a secondary metabolite having a benzene ring skeleton substituted at the para-position with a functional group containing a nitrogen atom. Another objective of the present invention is to provide a novel gene involved in the biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid. A novel gene according to the present invention comprises genes encoding the amino acid sequences of SEQ ID NOs: 2, 4 and 6 or modified sequences thereof.
摘要翻译：本发明的目的是提供改性的转化体，以便产生次级代谢物，其中次级产物的苯环在对位上用含氮原子的官能团改性。根据本发明的转化体是产生具有苯环骨架的次级代谢物的生物体的转化体，其在具有含氮原子的官能团的对位没有取代，所述转化体通过引入参与生物合成途径的基因而转化包括编码氨基酸序列（SEQ ID NO：4）的氨基酸序列（SEQ ID NO：2）的编码氨基酸序列（SEQ ID NO：4）的氨基酸序列（SEQ ID NO：2）的基因的氨基苯基丙酮酸，具有4-氨基-4-脱氧酸性变位酶活性的基因，以及编码具有4-氨基-4-脱氧蝶呤酸脱氢酶活性的氨基酸序列（SEQ ID NO：6）的基因，以产生具有苯环骨架的次级代谢物在对位上用含有氮原子的官能团取代。本发明的另一个目的是提供一种涉及从氯酸到对氨基苯基丙酮酸生物合成途径的新基因。根据本发明的新基因包含编码SEQ ID NO：2,4和6的氨基酸序列的基因或其修饰序列。

7. 发明申请

US20080197697A1 Hydraulic brake pressure control apparatus for vehicle 审中-公开
标题翻译：车辆液压制动压力控制装置
公开(公告)号：US20080197697A1
公开(公告)日：2008-08-21
申请号：US12071173
申请日：2008-02-15
申请人： Takahiro Naganuma , Koji Yanai
发明人： Takahiro Naganuma , Koji Yanai
IPC分类号： B60T8/50 , B60T8/34
CPC分类号： B60T8/3675
摘要： A hydraulic brake pressure control apparatus has a hydraulic unit having a housing, a casing fixed to the housing, and a circuit board arranged in the casing. A resilient member is disposed between the housing and the casing such that the housing and the casing are separated from each other, in order to reduce vibration transfer from the hydraulic unit to the circuit board. A part of the resilient member is also disposed between a bolt inserted into a through-hole formed in the resilient member and the casing.
摘要翻译：液压制动压力控制装置具有液压单元，其具有壳体，壳体固定到壳体以及布置在壳体中的电路板。弹性构件设置在壳体和壳体之间，使得壳体和壳体彼此分离，以便减少从液压单元到电路板的振动传递。弹性构件的一部分也设置在插入形成在弹性构件的通孔中的螺栓和壳体之间。

8. 发明授权

US06337201B1 &bgr;-fructofuranosidase and its gene, method of isolating &bgr;-fructofuranosidase gene, system for producing &bgr;-fructofuranosidase, and &bgr;-fructofuranosidase variant 有权
标题翻译： β-呋喃果糖苷酶及其基因，分离β-呋喃果糖苷酶基因的方法，β-呋喃果糖苷酶的制备和β-呋喃果糖苷酶变体
公开(公告)号：US06337201B1
公开(公告)日：2002-01-08
申请号：US09142623
申请日：1998-09-10
申请人： Koji Yanai , Akitaka Nakane , Hirofumi Nakamura , Yuko Baba , Akemi Watabe , Masao Hirayama
发明人： Koji Yanai , Akitaka Nakane , Hirofumi Nakamura , Yuko Baba , Akemi Watabe , Masao Hirayama
IPC分类号： C12N924
CPC分类号： C12Y302/01026 , C12N9/2431
摘要： A novel &bgr;-fructofuranosidase gene and a &bgr;-fructofuranosidase encoded by the gene, a process for isolating a &bgr;-fructofuranosidase gene using the novel &bgr;-fructofuranosidase gene, and a novel &bgr;-fructofuranosidase obtained by this isolation process are disclosed. A novel mold fungus having no &bgr;-fructofuranosidase activity suitable for the production of &bgr;-fructofuranosidase, and a system for producing a recombinant &bgr;-fructofuranosidase using the novel mold fungus as a host is disclosed. Further, a &bgr;-fructofuranosidase variant which selectively and efficiently produces a specific fructooligosaccharide such as 1-kestose from sucrose is disclosed.
摘要翻译：公开了一种新颖的β-呋喃果糖苷酶基因和由该基因编码的β-呋喃果糖苷酶，使用新的β-呋喃果糖苷酶基因分离β-呋喃果糖苷酶基因的方法和通过该分离方法获得的新型β-呋喃果糖苷酶。公开了一种不具有适用于β-呋喃果糖苷酶生产的β-呋喃果糖苷酶活性的新型霉菌，以及使用该新型霉菌作为宿主生产重组β-呋喃果糖苷酶的体系。此外，公开了一种β-蔗糖呋喃糖苷酶变体，其选择性和有效地产生特定的低聚果糖，例如来自蔗糖的1-kestose。

9. 发明授权

US07607924B2 Electronic control unit and method of manufacturing the same 有权
标题翻译：电子控制单元及其制造方法
公开(公告)号：US07607924B2
公开(公告)日：2009-10-27
申请号：US12022780
申请日：2008-01-30
申请人： Koji Yanai , Takayuki Shibata , Shoichi Ishikawa
发明人： Koji Yanai , Takayuki Shibata , Shoichi Ishikawa
IPC分类号： H01R12/00
CPC分类号： H05K7/026 , B60T8/3675 , H05K1/0263 , H05K3/3447 , H05K2201/09709 , H05K2201/10272 , Y10S439/949 , Y10T29/49117
摘要： An electronic control unit includes a circuit board, a case, and a group of a plurality of bus bars fixed to the case by insert molding. The bus bars each include a terminal portion having a constant-width portion, and a base portion which is wider than the constant-width portion. The base portions of the respective bus bars are arranged parallel to each other in the width direction thereof. Each bus bar is bent at a bent portion of its base portion. The terminal portion of each bus bar extends through the circuit board and is soldered to and electrically connected to a terminal connecting portion of an electrical circuit on the circuit board at a soldered portion. The bent portions of alternate bus bars are offset from the bent portions of the other bus bars in the longitudinal direction of the bus bars, whereby the soldered portions of the respective terminal portions are arranged in a staggered manner.
摘要翻译：电子控制单元包括电路板，壳体和通过插入成型固定到壳体的多个汇流条的组。汇流条各自包括具有恒定宽度部分的端子部分和比恒定宽度部分宽的基部部分。各母线的基部在宽度方向上彼此平行地配置。每个汇流条在其基部的弯曲部分处弯曲。每个母线的端子部分延伸穿过电路板，并焊接到电连接部分的电路板上的电路的端子连接部分并与之电连接。备用汇流条的弯曲部分在汇流条的长度方向上偏离其它汇流条的弯曲部分，由此相应端子部分的焊接部分以交错方式排列。

10. 发明授权

US07513482B2 Actuator for controlling brake hydraulic pressure and solenoid valve thereof 有权
标题翻译：用于控制制动液压的致动器及其电磁阀
公开(公告)号：US07513482B2
公开(公告)日：2009-04-07
申请号：US11589896
申请日：2006-10-31
申请人： Takayuki Shibata , Koji Yanai , Toru Fujita , Tomoaki Kawabata
发明人： Takayuki Shibata , Koji Yanai , Toru Fujita , Tomoaki Kawabata
IPC分类号： F16K31/02
CPC分类号： B60T8/363 , B60T8/368 , F16K27/003 , F16K31/0675 , Y10S251/903
摘要： A solenoid valve for use in an actuator for controlling brake hydraulic pressure includes a valve housing, a movable core having an integral valve rod and axially slidably received in the valve housing, and a valve body configured to be moved into and out of contact with a valve seat when the valve body moves. The solenoid valve further includes a yoke having a U-shaped section. The flanges of the yoke have first ends that extend substantially along the outer peripheral edges of the coil winding to minimize the length of the flanges and thus the length of the solenoid valve.
摘要翻译：一种用于控制制动液压的致动器的电磁阀包括阀壳体，具有一体式阀杆并可轴向可滑动地容纳在阀壳体中的可动芯体，以及被配置成移动进入和脱离接触的阀体阀体运动时阀座。电磁阀还包括具有U形截面的轭。轭的凸缘具有基本上沿着线圈绕组的外周缘延伸的第一端，以最小化凸缘的长度并因此使电磁阀的长度最小化。

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