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1. 发明授权

US07176002B2 Universal-tagged oligonucleotide primers and methods of use 有权
标题翻译：通用标记的寡核苷酸引物和使用方法
公开(公告)号：US07176002B2
公开(公告)日：2007-02-13
申请号：US10151061
申请日：2002-05-16
申请人： Kai Qin Lao , Caifu Chen , Ryan T. Koehler , Charles Scafe , Gary Schroth
发明人： Kai Qin Lao , Caifu Chen , Ryan T. Koehler , Charles Scafe , Gary Schroth
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C07H21/00 , C12Q1/686 , C12Q2525/179 , C12Q2525/15
摘要： The present invention relates to universal-tagged oligonucleotide primers, and to methods of using the primers for amplifying the genome.
摘要翻译：本发明涉及通用标记的寡核苷酸引物，以及使用引物扩增基因组的方法。

2. 发明申请

US20070128654A1 Universal-tagged oligonucleotide primers and methods of use 审中-公开
公开(公告)号：US20070128654A1
公开(公告)日：2007-06-07
申请号：US11655527
申请日：2007-01-18
申请人： Kai Lao , Caifu Chen , Ryan Koehler , Charles Scafe , Gary Schroth
发明人： Kai Lao , Caifu Chen , Ryan Koehler , Charles Scafe , Gary Schroth
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C07H21/00 , C12Q1/686 , C12Q2525/179 , C12Q2525/15
摘要： The present invention relates to universal-tagged oligonucleotide primers, and to methods of using the primers for amplifying the genome.

3. 发明申请

US20130011840A1 Methods, Compositions, and Kits Comprising Linker Probes for Quantifying Polynucleotides 审中-公开
标题翻译：包含用于定量多核苷酸的接头探针的方法，组合物和试剂盒
公开(公告)号：US20130011840A1
公开(公告)日：2013-01-10
申请号：US13612485
申请日：2012-09-12
申请人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
发明人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C07H21/04 , C12N9/00 , C12P19/34 , C12Q1/686 , C12Q2525/301 , C12Q2600/16 , C12Q2600/178
摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.
摘要翻译：本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。然后可以在多个扩增反应中解码多个查询的miRNA。

4. 发明申请

US20100112573A1 Methods, Compositions, and Kits Comprising Linker Probes for Quantifying Polynucleotides 有权
标题翻译：包含用于定量多核苷酸的接头探针的方法，组合物和试剂盒
公开(公告)号：US20100112573A1
公开(公告)日：2010-05-06
申请号：US12543466
申请日：2009-08-18
申请人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
发明人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C07H21/04 , C12N9/00 , C12P19/34 , C12Q1/686 , C12Q2525/301 , C12Q2600/16 , C12Q2600/178
摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.
摘要翻译：本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。然后可以在多个扩增反应中解码多个查询的miRNA。

5. 发明授权

US09068222B2 Methods compositions, and kits comprising linker probes for quantifying polynucleotides 有权
标题翻译：方法组合物和包含用于定量多核苷酸的连接探针的试剂盒
公开(公告)号：US09068222B2
公开(公告)日：2015-06-30
申请号：US12543466
申请日：2009-08-18
申请人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
发明人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
IPC分类号： C07H21/04 , C12N9/00 , C12Q1/68
CPC分类号： C12Q1/6876 , C07H21/04 , C12N9/00 , C12P19/34 , C12Q1/686 , C12Q2525/301 , C12Q2600/16 , C12Q2600/178
摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.
摘要翻译：本发明涉及用于鉴定和定量靶多核苷酸序列的方法，试剂，试剂盒和组合物。包含3'靶特异性部分，环和茎的接头探针与目标多核苷酸杂交并延伸以形成包含反向引物部分和茎核苷酸的反应产物。可以在扩增反应中使用检测器探针，特异性正向引物和反向引物，其中检测器探针可以基于由接头探针引入的干核苷酸检测扩增的靶多核苷酸。在一些实施方案中，使用多个接头探针查询多个短miRNA，其中所述连接物探针都包含不同3'靶特异性部分和不同茎的通用反向引物部分。然后可以在多个扩增反应中解码多个查询的miRNA。

6. 发明授权

US07208278B2 Concatameric ligation products: compositions, methods and kits for same 有权
标题翻译：聚合物连接产物：组合物，方法和试剂盒相同
公开(公告)号：US07208278B2
公开(公告)日：2007-04-24
申请号：US10982619
申请日：2004-11-04
申请人： Caifu Chen , Kevin Hennessy , Kai Qin Lao , Teodoro Paner , Vinod Mirchandani
发明人： Caifu Chen , Kevin Hennessy , Kai Qin Lao , Teodoro Paner , Vinod Mirchandani
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12P19/34 , C12Q1/6813 , C12Q1/6816 , C12Q2600/156 , C12Q2525/125 , C12Q2521/501 , C12Q2521/319 , C12Q2525/301 , C12Q2565/125 , C12Q2533/107
摘要： The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.
摘要翻译：本教导涉及用于检测样品中的一种或多种靶多核苷酸序列的方法，组合物和试剂盒，以及用于形成连续连接产物的方法组合物和试剂盒。在本教导的一些实施方案中，寡核苷酸与互补靶多核苷酸杂交并连接在一起以形成连接产物。在本教导的一些实施方案中，可以扩增连续连接产物，并且基于在连接反应中引入的序列确定靶多核苷酸的身份和数量。本教导的一些实施方案提供从反应混合物中除去未螯合的探针的方法。本教导的一些实施方案使用各种分析程序提供多重检测，鉴定和定量多个靶多核苷酸。

7. 发明申请

US20120190019A1 CONCATAMERIC LIGATION PRODUCTS: COMPOSITIONS METHODS AND KITS FOR SAME 审中-公开
标题翻译： CONCATAMERIC LIGATION产品：组合物方法和用途相同
公开(公告)号：US20120190019A1
公开(公告)日：2012-07-26
申请号：US13333302
申请日：2011-12-21
申请人： Caifu Chen , Kevin Hennessy , Kai Qin Lao , Teodoro Paner , Vinod Mirchandani
发明人： Caifu Chen , Kevin Hennessy , Kai Qin Lao , Teodoro Paner , Vinod Mirchandani
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q2533/107 , C12Q2525/301
摘要： The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.
摘要翻译：本教导涉及用于检测样品中的一种或多种靶多核苷酸序列的方法，组合物和试剂盒，以及用于形成连续连接产物的方法组合物和试剂盒。在本教导的一些实施方案中，寡核苷酸与互补靶多核苷酸杂交并连接在一起以形成连接产物。在本教导的一些实施方案中，可以扩增连续连接产物，并且基于在连接反应中引入的序列确定靶多核苷酸的身份和数量。本教导的一些实施方案提供从反应混合物中除去未螯合的探针的方法。本教导的一些实施方案使用各种分析程序提供多重检测，鉴定和定量多个靶多核苷酸。

8. 发明授权

US07575863B2 Methods, compositions, and kits comprising linker probes for quantifying polynucleotides 有权
公开(公告)号：US07575863B2
公开(公告)日：2009-08-18
申请号：US10947460
申请日：2004-09-21
申请人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
发明人： Caifu Chen , Dana Ridzon , Zhaohui Zhou , Kai Qin Lao , Neil A. Straus
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C07H21/04 , C12N9/00 , C12P19/34 , C12Q1/686 , C12Q2525/301 , C12Q2600/16 , C12Q2600/178
摘要： The present invention is directed to methods, reagents, kits, and compositions for identifying and quantifying target polynucleotide sequences. A linker probe comprising a 3′ target specific portion, a loop, and a stem is hybridized to a target polynucleotide and extended to form a reaction product that includes a reverse primer portion and the stem nucleotides. A detector probe, a specific forward primer, and a reverse primer can be employed in an amplification reaction wherein the detector probe can detect the amplified target polynucleotide based on the stem nucleotides introduced by the linker probe. In some embodiments a plurality of short miRNAs are queried with a plurality of linker probes, wherein the linker probes all comprise a universal reverse primer portion a different 3′ target specific portion and different stems. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.

9. 发明授权

US09534255B2 Methods, compositions, and kits for detecting allelic variants 有权
标题翻译：用于检测等位基因变体的方法，组合物和试剂盒
公开(公告)号：US09534255B2
公开(公告)日：2017-01-03
申请号：US12641321
申请日：2009-12-17
申请人： Caifu Chen , Ruoying Tan
发明人： Caifu Chen , Ruoying Tan
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6886 , C07H21/04 , C12Q1/6858 , C12Q2600/156 , C12Q2600/172 , C12Q2561/113 , C12Q2561/101 , C12Q2537/161
摘要： In some embodiments, the present inventions relates generally to compositions, methods and kits for use in discriminating sequence variation between different alleles. More specifically, in some embodiments, the present invention provides for compositions, methods and kits for quantitating rare (e.g., mutant) allelic variants, such as SNPs, or nucleotide (NT) insertions or deletions, in samples comprising abundant (e.g., wild type) allelic variants with high specificity and selectivity. In particular, in some embodiments, the invention relates to a highly selective method for mutation detection referred to as competitive allele-specific TaqMan PCR (“cast-PCR”).
摘要翻译：在一些实施方案中，本发明一般涉及用于区分不同等位基因之间的序列变异的组合物，方法和试剂盒。更具体地说，在一些实施方案中，本发明提供了用于定量稀少（例如，突变体）等位基因变体如SNP或核苷酸（NT）插入或缺失的组合物，方法和试剂盒，其包含丰富的（例如，野生型）等位基因变体，具有高特异性和选择性。特别地，在一些实施方案中，本发明涉及用于突变检测的高选择性方法，称为竞争性等位基因特异性TaqMan PCR（“cast-PCR”）。

10. 发明授权

US09309565B2 Karyotyping assay 有权
标题翻译：核型分析法
公开(公告)号：US09309565B2
公开(公告)日：2016-04-12
申请号：US13107786
申请日：2011-05-13
申请人： Adam Broomer , Kelly Li , Andreas R. Tobler , Caifu Chen , David N. Keys, Jr.
发明人： Adam Broomer , Kelly Li , Andreas R. Tobler , Caifu Chen , David N. Keys, Jr.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2537/16
摘要： This disclosure relates to methods and kits for karyotyping in which chromosomes are interrogated by amplifying loci that are not within copy number variable regions thereof.
摘要翻译：本公开涉及用于核型分析的方法和试剂盒，其中染色体通过扩增不在其拷贝数可变区内的位点进行询问。

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