专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明授权

US09458243B2 Secondary antibody detected epitope and preparation of same 有权
标题翻译：二抗检测表位及其制备方法
公开(公告)号：US09458243B2
公开(公告)日：2016-10-04
申请号：US14534174
申请日：2014-11-06
申请人： KAOHSIUNG MEDICAL UNIVERSITY
发明人： Tian-Lu Cheng , Steve Roffler , Wen-Wei Lin , I-Ju Chen
IPC分类号： C07K16/42 , C07K16/00
CPC分类号： G01N33/6803 , C07K14/00 , C07K16/00 , C07K16/4283 , C07K2317/52 , C07K2319/00 , C07K2319/40 , G01N33/58 , G01N33/6854 , G01N2570/00
摘要： A method of preparing an epitope for a secondary antibody detected protein tag, comprising: constructing a expressing vector, comprising a nucleotide sequence for encoding the secondary antibody detected protein tag comprising at least one epitope selected from at least one primary antibody which is detected by corresponding secondary antibody; expressing the vector in a host cell; and obtaining the secondary antibody detected protein tag which is expressed in the host cell. Further, a method for constructing a protein molecular weight marker ladder, comprising constructing a plurality of recombinant protein tags with different molecular weights, wherein each the recombinant protein tag comprises at least one epitope of primary antibody. Besides, a secondary antibody detected protein tag comprises at least two peptide sequences of epitopes selected from primary antibodies of at least two different species.
摘要翻译：一种制备二抗检测蛋白质标签的表位的方法，包括：构建表达载体，其包含用于编码第二抗体检测的蛋白质标签的核苷酸序列，所述核苷酸序列包含至少一个选自至少一个第一抗体的表位，所述表位被相应的二抗; 在宿主细胞中表达载体; 并获得在宿主细胞中表达的二抗检测蛋白质标签。此外，构建蛋白质分子量标记梯的方法，包括构建多个具有不同分子量的重组蛋白质标签，其中每个重组蛋白质标签包含一抗的至少一个表位。此外，二次抗体检测的蛋白质标签包含至少两个选自至少两种不同物种的一抗的表位的肽序列。

IPRDB

热门服务

关于我们

友情链接

联系方式