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1. 发明申请

US20180371509A1 PHA-PRODUCING MICROORGANISM HAVING SUCROSE ASSIMILABILITY, AND METHOD FOR PRODUCING PHA USING SAID MICROORGANISM 审中-公开
公开(公告)号：US20180371509A1
公开(公告)日：2018-12-27
申请号：US16061923
申请日：2016-12-14
申请人： KANEKA CORPORATION
发明人： Hisashi ARIKAWA , Tetsuya FUJIKI
IPC分类号： C12P7/62 , C08G63/06 , C12N9/10 , C12N15/52 , C12N1/20
摘要： An object of the present invention is to provide a PHA-producing microorganism which can assimilate sucrose, and a method for producing a PHA by culturing this microorganism, using sucrose as a carbon source. A PHA-producing microorganism, comprising a PHA synthase gene, and heterogeneous-organism-derived genes in the following items (1) and (2): (1) a sucrose hydrolase gene encoding an amino acid sequence described in SEQ ID NO: 1, or a gene encoding a polypeptide which has a sequence homology of 90% or more to the amino acid sequence and which has sucrose hydrolase activity; and (2) a sucrose permease gene encoding an amino acid sequence described in SEQ ID NO: 2, or a gene encoding a polypeptide which has a sequence homology of 90% or more to the amino acid sequence and which has sucrose permease activity. The invention is also a method for producing a PHA, including the step of culturing this microorganism in a medium including sucrose as a carbon source.

2. 发明申请

US20220411830A1 METHOD OF PRODUCING POLYHYDROXYALKANOATE 有权
公开(公告)号：US20220411830A1
公开(公告)日：2022-12-29
申请号：US17763886
申请日：2020-08-05
申请人： KANEKA CORPORATION
发明人： Hisashi ARIKAWA , Shunsuke SATO , Yoshihiro MOURI
IPC分类号： C12P7/625
摘要： A polyhydroxyalkanoate-producing microorganism is cultured to obtain microbial bodies accumulating polyhydroxyalkanoate particles and having an average cell size of 2 μm or more. The microbial bodies are subjected to a heat treatment at a temperature higher than a temperature in the culturing to increase an average particle size of the polyhydroxyalkanoate particles in the microbial bodies. The resulting average particle size is equal to or greater than 1.8 μm and equal to or smaller than the average cell size. The microbial bodies subjected to the heat treatment can be disrupted to obtain a cell disruption solution. The PHA particles can be separated from an aqueous phase of the cell disruption solution.

3. 发明申请

US20210388324A1 TRANSFORMED MICROORGANISM AND METHOD OF PRODUCING POLYHYDROXYALKANOATE 有权
公开(公告)号：US20210388324A1
公开(公告)日：2021-12-16
申请号：US17412340
申请日：2021-08-26
申请人： KANEKA CORPORATION
发明人： Hisashi ARIKAWA , Shunsuke SATO
IPC分类号： C12N9/10 , C12P7/62
摘要： Provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of a minD gene is enhanced. Also provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of a minC gene and a minD gene is enhanced. In this transformed microorganism, expression of a minE gene may be enhanced or reduced. Also provided is a method of producing a PHA, the method including the step of culturing any of the transformed microorganisms in the presence of a carbon source.

4. 发明申请

US20220315958A1 TRANSFORMED MICROORGANISM AND METHOD OF PRODUCING POLYHYDROXYALKANOATE USING THE MICROORGANISM 有权
公开(公告)号：US20220315958A1
公开(公告)日：2022-10-06
申请号：US17641358
申请日：2020-08-04
申请人： KANEKA CORPORATION
发明人： Yoshihiro MOURI , Hisashi ARIKAWA , Shunsuke SATO
IPC分类号： C12P7/625 , C12N1/20 , C12N15/74 , C12N9/10
摘要： Provided is a transformed microorganism that has a polyhydroxyalkanoate synthase gene and in which expression of an A1386 gene and/or an A2405 gene is reduced. In the transformed microorganism, expression of a minC gene and a minD gene may be enhanced. Also provided is a method of producing a PHA, the method including the step of culturing the transformed microorganism in the presence of a carbon source.

5. 发明申请

US20170198313A1 MICROORGANISM HAVING MULTIPLE GENES ENCODING PHA SYNTHASE AND METHOD FOR PRODUCING PHA USING SAME 审中-公开
公开(公告)号：US20170198313A1
公开(公告)日：2017-07-13
申请号：US15129726
申请日：2015-03-27
申请人： KANEKA CORPORATION
发明人： Shingo KOBAYASHI , Rina AOKI , Tetsuya MINAMI , Hisashi ARIKAWA , Keiji MATSUMOTO
IPC分类号： C12P7/62 , C12N9/88 , C12N9/10
CPC分类号： C12P7/625 , C08G63/06 , C08L101/16 , C12N9/1025 , C12N9/1029 , C12N9/88 , C12N15/09 , C12Y203/01 , C12Y402/01017
摘要： A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

6. 发明公开

US20240191266A1 METHOD OF PRODUCING POLYHYDROXYALKANOATE COPOLYMER MIXTURE AND TRANSFORMED MICROORGANISM 审中-公开
公开(公告)号：US20240191266A1
公开(公告)日：2024-06-13
申请号：US17995827
申请日：2021-04-08
申请人： KANEKA CORPORATION
发明人： Hisashi ARIKAWA , Shunsuke SATO , Sho FURUTATE
IPC分类号： C12P7/625 , C12N1/20 , C12N9/10 , C12N9/88 , C12N15/74
CPC分类号： C12P7/625 , C12N1/20 , C12N9/1029 , C12N9/88 , C12N15/74 , C12Y402/01017
摘要： Provided is a method of producing a polyhydroxyalkanoate copolymer mixture. The method includes the step of culturing a microorganism that produces the polyhydroxyalkanoate copolymer mixture. The mixture contains: a fraction (I) containing a polyhydroxyalkanoate copolymer having a 3-hydroxybutyrate structural unit and a 3-hydroxyhexanoate structural unit, the fraction (I) having an average 3-hydroxyhexanoate unit ratio of 20 mol % or more; and a fraction (II) containing a polyhydroxyalkanoate having a 3-hydroxybutyrate structural unit, the fraction (II) having an average 3-hydroxyhexanoate unit ratio of 0 to 15 mol %. The polyhydroxyalkanoate copolymer mixture has an average 3-hydroxyhexanoate unit ratio of 22 mol % or less.

7. 发明申请

US20200340020A1 TRANSFORMED MICROORGANISM FOR PRODUCING PHA COPOLYMER COMPRISING 3HH MONOMER UNIT AT HIGH COMPOSITION RATE AND METHOD FOR PRODUCING PHA USING SAME 审中-公开
公开(公告)号：US20200340020A1
公开(公告)日：2020-10-29
申请号：US16927229
申请日：2020-07-13
申请人： KANEKA CORPORATION
发明人： Hisashi ARIKAWA , Shunsuke Sato
IPC分类号： C12P7/62 , C12N9/10
摘要： The present application provides: a transformed microorganism for producing a PHA copolymer containing 3HH monomer unit at a higher composition ratio, specifically, a transformed microorganism comprising a PHA synthase gene capable of synthesizing a PHA copolymer containing 3HH monomer unit and a gene encoding a protein having (R)-specific enoyl-CoA hydratase activity, characterized in that, in the transformed microorganism, the expression of a gene encoding at least one β-ketothiolase enzyme having thiolysis activity for β-keto-(C6) acyl-CoA (i.e., β-ketohexanoyl-CoA) is inhibited, thereby losing or reducing the enzyme activity; and a method for producing a PHA copolymer containing 3HH monomer unit, comprising a step of culturing the transformed microorganism.

8. 发明申请

US20200056214A1 MICROORGANISM HAVING MULTIPLE GENES ENCODING PHA SYNTHASE AND METHOD FOR PRODUCING PHA USING SAME 审中-公开
公开(公告)号：US20200056214A1
公开(公告)日：2020-02-20
申请号：US16670042
申请日：2019-10-31
申请人： KANEKA CORPORATION
发明人： Shingo KOBAYASHI , Rina AOKI , Tetsuya MINAMI , Hisashi ARIKAWA , Keiji MATSUMOTO
IPC分类号： C12P7/62 , C12N9/88 , C12N9/10 , C08G63/06
摘要： A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

9. 发明申请

US20170009221A1 MICROORGANISM HAVING REGULATED EXPRESSION OF (R)-SPECIFIC ENOYL-COA HYDRATASE GENE AND METHOD FOR PRODUCING POLYHYDROXYALKANOATE COPOLYMER USING SAME 审中-公开
标题翻译：具有（R） - 优选的ENOYL-COA水解酶基因的调节表达的微生物和使用该方法生产聚羟基烷基酯共聚物的方法
公开(公告)号：US20170009221A1
公开(公告)日：2017-01-12
申请号：US15115394
申请日：2015-01-30
申请人： KANEKA CORPORATION
发明人： Hisashi ARIKAWA , Shunsuke SATO , Keiji MATSUMOTO
IPC分类号： C12N9/88 , C12P7/62 , C12N15/74
CPC分类号： C12N9/88 , C12N15/74 , C12P7/625 , C12Y402/01017 , C12Y402/01074
摘要： This invention relates to a microorganism that produces a polyhydroxyalkanoate (PHA) copolymer with a regulated monomer composition ratio and comprises a (R)-specific enoyl-CoA hydratase gene in the genome DNA, wherein a nucleotide sequence upstream of the (R)-specific enoyl-CoA hydratase gene comprises a modification consisting of a substitution(s), a deletion(s), an insertion(s), and/or an addition(s) of one or a plurality of nucleotides so that the expression of the (R)-specific enoyl-CoA hydratase gene is regulated, and to a method for producing a PHA copolymer using the microorganism.
摘要翻译：本发明涉及产生具有调节的单体组成比的聚羟基链烷酸酯（PHA）共聚物的微生物，并且在基因组DNA中包含（R） - 特异性烯酰辅酶A水合酶基因，其中（R） - 特异性烯酰辅酶A水合酶基因包含由一个或多个核苷酸的取代，缺失，插入和/或添加组成的修饰物，使得（ R） - 特异性烯酰辅酶A水合酶基因，以及使用该微生物生产PHA共聚物的方法。

10. 发明申请

US20160237462A1 HIGH MOLECULAR WEIGHT PHA-PRODUCING MICROBE AND METHOD OF PRODUCING HIGH MOLECULAR WEIGHT PHA USING SAME 审中-公开
标题翻译：高分子量生产微生物及其生产高分子量PHA的方法
公开(公告)号：US20160237462A1
公开(公告)日：2016-08-18
申请号：US14437672
申请日：2013-10-22
申请人： KANEKA CORPORATION
发明人： Hisashi ARIKAWA , Shunsuke SATO , Keiji MATSUMOTO
IPC分类号： C12P7/62 , C12N15/74
CPC分类号： C12P7/625 , C12N9/18 , C12N15/74
摘要： An object of the present invention is to provide a microorganism strain that accumulates a high molecular weight PHA, and a PHA production method using the microorganism. The present invention provides a method for producing a PHA copolymer, which includes culturing a microorganism, wherein at least a portion of either of the following genes (a) and (b) of the microorganism has been altered by substitution, deletion, insertion, and/or addition to reduce or eliminate the activity of a PHA degrading enzyme encoded by the gene: (a) a PHA degrading enzyme gene encoding the amino acid sequence of SEQ ID NO:2 in the sequence listing; and (b) a gene encoding a polypeptide having at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 in the sequence listing and having PHA degrading enzyme activity.
摘要翻译：本发明的目的是提供一种积累高分子量PHA的微生物菌株和使用该微生物的PHA生产方法。本发明提供一种生产PHA共聚物的方法，其包括培养微生物，其中微生物的以下基因（a）和（b）中的至少一部分已经被替换，缺失，插入和 /或添加以减少或消除由该基因编码的PHA降解酶的活性：（a）编码序列表中SEQ ID NO：2的氨基酸序列的PHA降解酶基因; 和（b）编码与序列表中SEQ ID NO：2的氨基酸序列具有至少85％序列同一性且具有PHA降解酶活性的多肽的基因。

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