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1. 发明授权

US08067561B2 Isolated DNA encoding recombinant glucose isomerase 有权
标题翻译：分离的DNA编码重组葡萄糖异构酶
公开(公告)号：US08067561B2
公开(公告)日：2011-11-29
申请号：US12723918
申请日：2010-03-15
申请人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
IPC分类号： C12N9/00 , C12N9/92 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/92 , C12P19/24
摘要： This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
摘要翻译：本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。这些重组葡萄糖异构酶包括氨基酸变异，包括139位的苯丙氨酸（Phe），182位的丙氨酸（Ala），187位的丝氨酸（Ser）和299位的谷氨酰胺（Gin），并携带至少一种其它的突变氨基在第87位，217位，260位或276位具有较高的酸性，当使用D-葡萄糖作为底物时，其具有比野生型更高的催化活性。这些重组葡萄糖异构酶可用于直接生产含有55％（重量）或更高浓度果糖的高果糖玉米糖浆。

2. 发明授权

US07704719B2 Glucose isomerase mutants, DNA thereof and use thereof 有权
标题翻译：葡萄糖异构酶突变体，其DNA及其用途
公开(公告)号：US07704719B2
公开(公告)日：2010-04-27
申请号：US12093859
申请日：2006-10-30
申请人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
IPC分类号： C12N9/00 , C12N9/92 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/92 , C12P19/24
摘要： This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.
摘要翻译：本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。这些重组葡萄糖异构酶包括氨基酸变异，包括139位的苯丙氨酸（Phe），182位的丙氨酸（Ala），187位的丝氨酸（Ser）和299位的谷氨酰胺（Gln），并携带至少一种另外的突变氨基在第87位，217位，260位或276位具有较高的酸性，当使用D-葡萄糖作为底物时，其具有比野生型更高的催化活性。这些重组葡萄糖异构酶可用于直接生产含有55％（wt）或更高浓度果糖的高果糖玉米糖浆。

3. 发明授权

US07919300B2 Glucose isomerase mutants 有权
标题翻译：葡萄糖异构酶突变体
公开(公告)号：US07919300B2
公开(公告)日：2011-04-05
申请号：US11597609
申请日：2004-07-28
申请人： Jun Wang , Rongzhao Fu , Dong Shen , Caike Jin , Zhangming Liu , Junming Chen
发明人： Jun Wang , Rongzhao Fu , Dong Shen , Caike Jin , Zhangming Liu , Junming Chen
IPC分类号： C12N9/92 , C12P19/02 , C07H21/04
CPC分类号： C12N9/92
摘要： This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt % or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt % fructose.
摘要翻译：本发明描述了具有改进的催化活性和热稳定性的一系列重组的热酵母杆菌葡糖异构酶。重组葡萄糖异构酶可用于直接生产含有55重量％或更高浓度果糖的果糖糖浆，或用于生产含有小于55重量％果糖的果糖糖浆。

4. 发明申请

US20080305529A1 Glucose Isomerase Mutants, Dna Thereof and Use Thereof 有权
标题翻译：葡萄糖异构酶突变体及其用途
公开(公告)号：US20080305529A1
公开(公告)日：2008-12-11
申请号：US12093859
申请日：2006-10-30
申请人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
IPC分类号： C12P19/24 , C12N9/92 , C07H21/04
CPC分类号： C12N9/92 , C12P19/24
摘要： This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.
摘要翻译：本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。这些重组葡萄糖异构酶包含位置139的氨基酸变异，包括苯丙氨酸Phe），182位丙氨酸（Ala），第187位的丝氨酸（Ser）和299位的谷氨酰胺（Gln），并携带至少一种另外的突变的氨基酸在位置87，位置217，位置260或位置276，并且当使用D-葡萄糖作为底物时，具有比野生型更高的催化活性。这些重组葡萄糖异构酶可用于直接生产含有55％（wt）或更高浓度果糖的高果糖玉米糖浆。

5. 发明授权

US07923222B2 Methods of using isolated glucose isomerase 有权
标题翻译：使用分离的葡萄糖异构酶的方法
公开(公告)号：US07923222B2
公开(公告)日：2011-04-12
申请号：US12723833
申请日：2010-03-15
申请人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
IPC分类号： C12P19/24 , C12N9/00 , C12N9/92 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/92 , C12P19/24
摘要： This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
摘要翻译：本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。这些重组葡萄糖异构酶包括氨基酸变异，包括139位的苯丙氨酸（Phe），182位的丙氨酸（Ala），187位的丝氨酸（Ser）和299位的谷氨酰胺（Gin），并携带至少一种其它的突变氨基在第87位，217位，260位或276位具有较高的酸性，当使用D-葡萄糖作为底物时，其具有比野生型更高的催化活性。这些重组葡萄糖异构酶可用于直接生产含有55％（重量）或更高浓度果糖的高果糖玉米糖浆。

6. 发明申请

US20100228016A1 DNA comprising a DNA SEQ. of isolated glucose isomerase 有权
标题翻译： DNA包含DNA SEQ。的葡萄糖异构酶
公开(公告)号：US20100228016A1
公开(公告)日：2010-09-09
申请号：US12723918
申请日：2010-03-15
申请人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
IPC分类号： C07H21/04
CPC分类号： C12N9/92 , C12P19/24
摘要： This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
摘要翻译：本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。这些重组葡萄糖异构酶包括氨基酸变异，包括139位的苯丙氨酸（Phe），182位的丙氨酸（Ala），187位的丝氨酸（Ser）和299位的谷氨酰胺（Gln），并携带至少一种另外的突变氨基在第87位，217位，260位或276位具有较高的酸性，当使用D-葡萄糖作为底物时，其具有比野生型更高的催化活性。这些重组葡萄糖异构酶可用于直接生产含有55％（重量）或更高浓度果糖的高果糖玉米糖浆。

7. 发明申请

US20100227365A1 Methods of using isolated glucose isomerase 有权
标题翻译：使用分离的葡萄糖异构酶的方法
公开(公告)号：US20100227365A1
公开(公告)日：2010-09-09
申请号：US12723833
申请日：2010-03-15
申请人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
发明人： Jun Wang , Caike Jin , Rongzhao Fu , Dong Shen
IPC分类号： C12P19/24
CPC分类号： C12N9/92 , C12P19/24
摘要： This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gin) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55% [wt] or higher concentration of fructose.
摘要翻译：本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡萄糖异构酶。这些重组葡萄糖异构酶包括氨基酸变异，包括139位的苯丙氨酸（Phe），182位的丙氨酸（Ala），187位的丝氨酸（Ser）和299位的谷氨酰胺（Gin），并携带至少一种其它的突变氨基在第87位，217位，260位或276位具有较高的酸性，当使用D-葡萄糖作为底物时，其具有比野生型更高的催化活性。这些重组葡萄糖异构酶可用于直接生产含有55％（重量）或更高浓度果糖的高果糖玉米糖浆。

8. 发明申请

US20090203080A1 S-Adenosylmethionine Synthetase Mutants, the DNAs Encoding the Same and Uses of the Mutants 有权
标题翻译： S-腺苷甲硫氨酸合成酶突变体，编码它们的DNA和突变体的用途
公开(公告)号：US20090203080A1
公开(公告)日：2009-08-13
申请号：US12438680
申请日：2007-08-30
申请人： Rongzhao Fu , Tianzuo Zhang , Jun Wang
发明人： Rongzhao Fu , Tianzuo Zhang , Jun Wang
IPC分类号： C12P19/40 , C12N9/00 , C07H21/00
CPC分类号： C12N9/1085 , C12Y205/01006
摘要： The invention discloses a series of Methanococcus jannaschii S-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.
摘要翻译：本发明公开了一种通过使用基因突变技术获得的具有改善的热稳定性和高催化活性的甲烷球菌S-腺苷甲硫氨酸合成酶突变体，其特征在于，这些突变体是指使用序列表中的序列2作为参考序列的酶，位置102，位置93，位置230和位置357处的至少一个突变，并且具有比使用三磷酸腺苷（ATP）和甲硫氨酸作为底物的野生型S-腺苷甲硫氨酸合成酶高至少70％的催化活性。这些S-腺苷甲硫氨酸合成酶突变体可用于S-腺苷甲硫氨酸的生产。

9. 发明授权

US07858351B2 S-adenosylmethionine synthetase mutants, the DNAs encoding the same and uses of the mutants 有权
标题翻译： S-腺苷甲硫氨酸合成酶突变体，编码相同的DNA和突变体的用途
公开(公告)号：US07858351B2
公开(公告)日：2010-12-28
申请号：US12438680
申请日：2007-08-30
申请人： Rongzhao Fu , Tianzuo Zhang , Jun Wang
发明人： Rongzhao Fu , Tianzuo Zhang , Jun Wang
IPC分类号： C12P19/40 , C12N9/10 , C12Q1/48
CPC分类号： C12N9/1085 , C12Y205/01006
摘要： The invention discloses a series of Methanococcus jannaschii S-adenosylmethionine synthetase mutants with improved thermostability and high catalytic activity obtained by using gene mutation technique, characterized in that these mutants refer to an enzyme using Sequence 2 in the Sequence Listing as the reference sequence and contains at least one mutation at position 102, position 93, position 230, and position 357 and has a catalytic activity at least 70% higher than that of the wild-type S-adenosylmethionine synthetase using adenosine triphosphate (ATP) and methionine as substrates. These S-adenosylmethionine synthetase mutants can be used in the production of S-adenosylmethionine.
摘要翻译：本发明公开了一种通过使用基因突变技术获得的具有改善的热稳定性和高催化活性的甲烷球菌S-腺苷甲硫氨酸合成酶突变体，其特征在于，这些突变体是指使用序列表中的序列2作为参考序列的酶，位置102，位置93，位置230和位置357处的至少一个突变，并且具有比使用三磷酸腺苷（ATP）和甲硫氨酸作为底物的野生型S-腺苷甲硫氨酸合成酶高至少70％的催化活性。这些S-腺苷甲硫氨酸合成酶突变体可用于S-腺苷甲硫氨酸的生产。

10. 发明授权

US08012726B2 Method of making bioethanol by using glucose isomerase mutants 有权
标题翻译：使用葡萄糖异构酶突变体制备生物乙醇的方法
公开(公告)号：US08012726B2
公开(公告)日：2011-09-06
申请号：US12093881
申请日：2006-10-30
申请人： Jun Wang , Rongzhao Fu , Tin Cho Cheung
发明人： Jun Wang , Rongzhao Fu , Tin Cho Cheung
IPC分类号： C12P7/06 , C12N9/00 , C12N9/90 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/92 , C12P7/10 , C12P19/24 , Y02E50/16 , Y02E50/17
摘要： This invention provides use of a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity obtained by using recombinant techniques. These mutants comprise at least one amino acid variation at position 87, position 139, position 182, position 187, position 217, position 260, position 276, or position 299, and can be used in the conversion of hemicellulose to ethanol.
摘要翻译：本发明提供了一系列使用重组技术获得的具有改进的催化活性的重组热酵母杆菌葡萄糖异构酶的用途。这些突变体包含位置87，位置139，位置182，位置187，位置217，位置260，位置276或位置299处的至少一个氨基酸变异，并且可以用于将半纤维素转化成乙醇。

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