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1. 发明申请

US20080069932A1 Oil or Fat Composition 审中-公开
标题翻译：油或脂肪组成
公开(公告)号：US20080069932A1
公开(公告)日：2008-03-20
申请号：US11579148
申请日：2005-03-30
申请人： Jun Kohori , Masao Shimizu , Shin Koike , Keiji Shibata , Hiroaki Yamaguchi , Minoru Kase , Yoshitaka Senda , Manabu Sato , Eizo Maruyama , Hidenari Imai , Tsutomu Nishide , Nobuteru Ishizuka
发明人： Jun Kohori , Masao Shimizu , Shin Koike , Keiji Shibata , Hiroaki Yamaguchi , Minoru Kase , Yoshitaka Senda , Manabu Sato , Eizo Maruyama , Hidenari Imai , Tsutomu Nishide , Nobuteru Ishizuka
IPC分类号： A23D7/01 , A23K1/00 , A61K31/075 , C11B5/00
CPC分类号： C11C1/045 , A23D9/007 , A23D9/013 , A23K20/158 , A23L33/115 , A23L33/12
摘要： Provided is an oil or fat composition of high diacylglycerol content. Even when used in cooking, the cooked food has a good external appearance without darkening, and retains its inherent flavor. Even when used in cooking after storage under light-exposed conditions, the cooked food is provided with a good external appearance and flavor. The oil or fat composition is obtained by combining, at specific contents, (A) an oil or fat of high diacylglycerol content, said oil or fat having a particular composition, (B) a plant sterol and (C) a plant sterol fatty acid ester.
摘要翻译：提供高二酰基甘油含量的油脂组合物。即使在烹饪中使用时，熟食也具有良好的外观，而不会变暗，并保持其固有的味道。即使在光照条件下储存后用于烹饪时，熟食也具有良好的外观和风味。通过以特定含量组合（A）具有高二酰基甘油含量的油或脂肪，所述油或脂肪具有特定组成，（B）植物甾醇和（C）植物甾醇脂肪酸酯。

2. 发明授权

US08241875B2 Method for producing fatty acids with an immobilized enzyme packed column 失效
标题翻译：用固定化酶填充柱生产脂肪酸的方法
公开(公告)号：US08241875B2
公开(公告)日：2012-08-14
申请号：US11450356
申请日：2006-06-12
申请人： Jun Saito , Yoshitaka Senda , Toshiteru Komatsu
发明人： Jun Saito , Yoshitaka Senda , Toshiteru Komatsu
IPC分类号： C12P7/64 , C12N11/14 , C12N11/02 , C12N11/08
CPC分类号： C11C1/045 , C12P7/6418
摘要： A process for producing fatty acids, which comprises supplying an oil phase substrate and a water phase substrate to an enzyme column packed with an immobilized enzyme, concurrently flowing them in the same direction, and hydrolyzing a fat and/or oil, wherein the oil phase substrate and the water phase substrate are alternately supplied to the enzyme column.
摘要翻译：一种生产脂肪酸的方法，其包括将油相底物和水相底物供给到装有固定化酶的酶柱，同时沿相同方向流动，并水解脂肪和/或油，其中所述油相底物和水相底物交替供应到酶柱。

3. 发明申请

US20090298142A1 METHOD FOR PRODUCING A USEFUL SUBSTANCE BY USE OF AN IMMOBILIZED ENZYME 失效
标题翻译：通过使用固定化酶生产有用物质的方法
公开(公告)号：US20090298142A1
公开(公告)日：2009-12-03
申请号：US12067664
申请日：2006-10-04
申请人： Jun Saito , Yoshitaka Senda , Toshiteru Komatsu
发明人： Jun Saito , Yoshitaka Senda , Toshiteru Komatsu
IPC分类号： C12P7/64 , C12P1/00
CPC分类号： C12N9/20 , C12N11/00 , C12P7/6418
摘要： The present invention provides a method for producing a useful substance by supplying, to a fixed-bed reactor packed with an immobilized enzyme, a liquid mixture containing two liquid phases, in which the two liquid phases are allowed to flow in an identical, parallel direction, which method employs a fixed-bed reactor equipped with an insertion unit or tubes, so as to form a plurality of lumens in the fixed-bed reactor, each lumen having a cross section of a circular shape with a diameter of 100 mm or less or having a polygonal shape with a diagonal line of 100 mm or less, wherein the lumens are packed with an immobilized enzyme and the liquid mixture is supplied therethrough. In a reaction performed by passing a reaction mixture exhibiting two liquid phases through a fixed-bed reactor equipped with an immobilized enzyme, overall flow of the reaction liquid is made uniform, to thereby facilitate production of a useful substance in an efficient manner.
摘要翻译：本发明提供一种制造有用物质的方法，该方法通过向固定化酶反应器中填充固定化酶，提供含有两个液相的液体混合物，其中允许两个液相沿相同的平行方向流动该方法采用装备有插入单元或固定床的固定床反应器，以在固定床反应器中形成多个内腔，每个管腔具有直径为100mm以下的圆形截面或具有对角线为100mm或更小的多边形形状，其中，腔内填充有固定化酶，并且通过其提供液体混合物。在通过使具有两个液相的反应混合物通过装有固定化酶的固定床反应器进行的反应中，使反应液体的整体流动均匀，从而有效地制备有用物质。

4. 发明申请

US20100047883A1 PROCESS FOR PRODUCING USEFUL SUBSTANCE USING IMMOBILIZED ENZYME 失效
标题翻译：使用固定化学品生产有用物质的方法
公开(公告)号：US20100047883A1
公开(公告)日：2010-02-25
申请号：US12517574
申请日：2007-12-14
申请人： Yoshitaka Senda , Jun Saito , Keigo Hanaki , Toshiteru Komatsu
发明人： Yoshitaka Senda , Jun Saito , Keigo Hanaki , Toshiteru Komatsu
IPC分类号： C12P7/64 , C12M1/00
CPC分类号： C12P7/40 , C12N11/00 , C12P7/62
摘要： The present invention relates to a process for producing a useful substance, the process including feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and allowing the liquid mixture to flow in the same direction in a co-current manner to perform a reaction, wherein the packing thickness of the immobilized enzyme per stage of the fixed bed-type reaction column having an equivalent circular diameter of 35 mmφ or larger is 10 to 200 mm.In the process for producing a useful substance, which is performed by feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, the flow of the reaction liquid inside the column can be made uniform, and as a result, reactivity and productivity can be enhanced.
摘要翻译：本发明涉及一种生产有用物质的方法，该方法包括将由两个液相形成的液体混合物进料到装有固定化酶的固定床型反应塔中，并使液体混合物沿相同方向流动以同时的方式进行反应，其中每个固定床型反应塔的固定化酶的包装厚度具有等效的圆直径为35mm＆phgr; 以上为10〜200mm。在通过将由两个液相形成的液体混合物进料到填充有固定化酶的固定床型反应塔中进行的有用物质的制造方法中，能够使柱内的反应液的流动均匀，结果可以提高反应性和生产率。

5. 发明授权

US08377664B2 Method for producing a useful substance by use of an immobilized enzyme 失效
标题翻译：通过使用固定化酶生产有用物质的方法
公开(公告)号：US08377664B2
公开(公告)日：2013-02-19
申请号：US12067664
申请日：2006-10-04
申请人： Jun Saito , Yoshitaka Senda , Toshiteru Komatsu
发明人： Jun Saito , Yoshitaka Senda , Toshiteru Komatsu
IPC分类号： C12P7/64 , C12N11/00 , C12N11/14
CPC分类号： C12N9/20 , C12N11/00 , C12P7/6418
摘要： The present invention provides a method for producing a useful substance by supplying, to a fixed-bed reactor packed with an immobilized enzyme, a liquid mixture containing two liquid phases, in which the two liquid phases are allowed to flow in an identical, parallel direction, which method employs a fixed-bed reactor equipped with an insertion unit or tubes, so as to form a plurality of lumens in the fixed-bed reactor, each lumen having a cross section of a circular shape with a diameter of 100 mm or less or having a polygonal shape with a diagonal line of 100 mm or less, wherein the lumens are packed with an immobilized enzyme and the liquid mixture is supplied. In a reaction performed by passing a reaction mixture exhibiting two liquid phases through a fixed-bed reactor equipped with an immobilized enzyme, overall flow of the reaction liquid is made uniform.
摘要翻译：本发明提供一种制造有用物质的方法，该方法通过向固定化酶反应器中填充固定化酶，提供含有两个液相的液体混合物，其中允许两个液相沿相同的平行方向流动该方法采用装备有插入单元或固定床的固定床反应器，以在固定床反应器中形成多个内腔，每个内腔具有直径为100mm或更小的圆形截面或具有对角线为100mm或更小的多边形形状，其中，腔内填充固定化酶并提供液体混合物。在通过使具有两个液相的反应混合物通过装有固定化酶的固定床反应器进行的反应中，使反应液体的整体流动均匀。

6. 发明申请

US20100041114A1 PROCESS FOR PRODUCING USEFUL SUBSTANCE WITH IMMOBILIZED ENZYME 失效
标题翻译：生产有益物质的方法，具有固定的酶
公开(公告)号：US20100041114A1
公开(公告)日：2010-02-18
申请号：US12518285
申请日：2007-12-14
申请人： Jun Saito , Yoshitaka Senda , Keigo Hanaki , Toshiteru Komatsu
发明人： Jun Saito , Yoshitaka Senda , Keigo Hanaki , Toshiteru Komatsu
IPC分类号： C12P7/64
CPC分类号： C12P7/6418 , C12N11/00
摘要： The present invention relates to a process for producing a useful substance, which includes feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and performing a reaction while allowing the liquid mixture to flow in the same direction in a co-current manner, wherein the fixed bed-type reaction column has partition plates inserted in the longitudinal direction of the fixed bed-type reaction column so as to form a plurality of tube-shaped structures, wherein each tube-shaped structure has a lateral cross-section which is circular or polygonal in shape with at least a part being unclosed and has a representative length of 100 mm or less, wherein the immobilized enzyme is packed into the tube-shaped structures, and wherein the liquid mixture is fed into the tube-shaped structures.Even when a reaction column having a large column diameter is used in the reaction performed by feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, the flow of the whole reaction liquid inside the column can be made uniform, and the enzymatic activity can be effectively appeared. As a result, reactivity and productivity can be enhanced. Particularly, in the hydrolysis of oils and fats, the enzymatic activity can be effectively appeared, so that fatty acids can be effectively produced. Furthermore, the operability at the time of removing the immobilized enzyme packed in the reaction column is good.
摘要翻译：本发明涉及一种生产有用物质的方法，包括将由两个液相形成的液体混合物进料到装有固定化酶的固定床型反应塔中，并进行反应同时使液体混合物流入相同的方向，其中固定床型反应塔具有沿固定床型反应塔的纵向方向插入的隔板，以形成多个管状结构，所述圆形结构具有圆形或多边形的横截面，其至少一部分未封闭并且具有100mm或更小的代表性长度，其中所述固定化酶被包装在所述管状结构体中，并且其中所述液体混合物被送入管状结构。即使在通过将由两个液相形成的液体混合物进料到装有固定化酶的固定床型反应塔中进行的反应中使用具有大柱直径的反应塔时，柱内的整个反应液体的流动可以均匀，可以有效地出现酶活性。因此，可以提高反应性和生产率。特别是在油脂的水解中，能够有效地发挥酶活性，能够有效地制造脂肪酸。此外，除去反应塔中填充的固定化酶时的操作性良好。

7. 发明授权

US07001749B2 Regenerating method of immobilized enzyme 失效
标题翻译：固定化酶的再生方法
公开(公告)号：US07001749B2
公开(公告)日：2006-02-21
申请号：US10654890
申请日：2003-09-05
申请人： Toshiteru Komatsu , Yoshitaka Senda , Goro Ujita
发明人： Toshiteru Komatsu , Yoshitaka Senda , Goro Ujita
IPC分类号： C12P7/64
CPC分类号： C12N11/00
摘要： Provided is a method for regenerating an immobilized enzyme for lipolysis which has been used for lipolysis, thereby exhibiting a reduced activity, which includes washing the immobilized enzyme with a solvent; controlling an equilibrium concentration of fatty acids in the solvent; removing the washed immobilized enzyme therefrom, and contacting the resulting immobilized enzyme with a fresh enzyme, wherein the fresh enzyme adsorbs onto the immobilized enzyme. According to the preferred methods of the present invention, it is possible to make effective use of the residual activity of an immobilized enzyme spent for lipolysis and regenerate the spent immobilized enzyme to have a performance similar to that before the lipolysis at a low cost using less amount of liquid waste.
摘要翻译：本发明提供一种再利用脂肪分解用固定化酶的方法，其用于脂肪分解，从而表现出降低的活性，其包括用溶剂洗涤固定化酶; 控制溶剂中脂肪酸的平衡浓度; 从其中除去洗涤的固定化酶，并使得到的固定化酶与新鲜酶接触，其中新鲜酶吸附到固定化酶上。根据本发明的优选方法，可以有效利用用于脂肪分解的固定化酶的残留活性，并使用较少的成本再生废消化的固定化酶以具有与脂肪分解之前相似的性能液体废物量。

8. 发明授权

US08252560B2 Process for producing useful substance with immobilized enzyme 失效
标题翻译：用固定化酶生产有用物质的方法
公开(公告)号：US08252560B2
公开(公告)日：2012-08-28
申请号：US12518285
申请日：2007-12-14
申请人： Jun Saito , Yoshitaka Senda , Keigo Hanaki , Toshiteru Komatsu
发明人： Jun Saito , Yoshitaka Senda , Keigo Hanaki , Toshiteru Komatsu
IPC分类号： C12P7/64 , C12N11/00 , C12N11/14
CPC分类号： C12P7/6418 , C12N11/00
摘要： The present invention relates to a process for producing a fatty acid, which includes feeding a liquid mixture formed of two liquid phases that flow in a co-current manner into a fixed bed-type reaction column packed with immobilized lipase, and performing a hydrolysis reaction when the two liquid phases are in contact with the immobilized lipase in the fixed-bed reaction column; wherein the fixed-bed reaction column comprises partition plates so as to comprise a plurality of tube- shaped structures, wherein each tube-shaped structure has a cross -section which is rectangular, circular, oval, or polygonal in shape with at least a part being unclosed and the cross-section has a representative length of 100 mm or less.
摘要翻译：本发明涉及一种脂肪酸的制造方法，其特征在于，将由以流动方式流动的两个液相形成的液体混合物输送到填充有固定化脂肪酶的固定床型反应塔中，进行水解反应当两个液相与固定床反应塔中的固定化脂肪酶接触时; 其中所述固定床反应塔包括分隔板，以便包括多个管状结构，其中每个管状结构具有矩形，圆形，椭圆形或多边形形状的横截面，至少部分未封闭，横截面长度为100mm以下。

9. 发明授权

US08173403B2 Process for producing useful substance using immobilized enzyme 失效
标题翻译：使用固定化酶生产有用物质的方法
公开(公告)号：US08173403B2
公开(公告)日：2012-05-08
申请号：US12517574
申请日：2007-12-14
申请人： Yoshitaka Senda , Jun Saito , Keigo Hanaki , Toshiteru Komatsu
发明人： Yoshitaka Senda , Jun Saito , Keigo Hanaki , Toshiteru Komatsu
IPC分类号： C12P7/64 , C12N11/00 , C12N11/14
CPC分类号： C12P7/40 , C12N11/00 , C12P7/62
摘要： The present invention relates to a process for producing a useful substance, the process includes feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and allowing the liquid mixture to flow in the same direction in a co-current manner to perform a reaction. The packing thickness of the immobilized enzyme per stage of the fixed bed-type reaction column having an equivalent circular diameter of 35 mmφ or larger is 10 to 200 mm. The flow of the reaction liquid inside the column can be made uniform, and as a result, reactivity and productivity is enhanced.
摘要翻译：本发明涉及一种生产有用物质的方法，该方法包括将由两个液相形成的液体混合物进料到装有固定化酶的固定床型反应塔中，并使液体混合物沿相同方向流动以同时的方式进行反应。固定床型反应塔每个阶段的固定化酶的包装厚度为等效圆直径为35mm＆phgr; 以上为10〜200mm。可以使柱内的反应液的流动均匀，结果提高了反应性和生产率。

10. 发明申请

US20060292675A1 Process for producing fatty acids 失效
标题翻译：生产脂肪酸的方法
公开(公告)号：US20060292675A1
公开(公告)日：2006-12-28
申请号：US11450356
申请日：2006-06-12
申请人： Jun Saito , Yoshitaka Senda , Toshiteru Komatsu
发明人： Jun Saito , Yoshitaka Senda , Toshiteru Komatsu
IPC分类号： C12P7/64
CPC分类号： C11C1/045 , C12P7/6418
摘要： A process for producing fatty acids, which comprises supplying an oil phase substrate and a water phase substrate to an enzyme column packed with an immobilized enzyme, concurrently flowing them in the same direction, and hydrolyzing a fat and/or oil, wherein the oil phase substrate and the water phase substrate are alternately supplied to the enzyme column.
摘要翻译：一种生产脂肪酸的方法，其包括将油相底物和水相底物供给到装有固定化酶的酶柱，同时沿相同方向流动，并水解脂肪和/或油，其中所述油相底物和水相底物交替供应到酶柱。

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