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1. 发明申请

US20120225431A1 METHODS OF SYNTHESIZING AND LABELING NUCLEIC ACID MOLECULES 审中-公开
标题翻译：合成和标记核酸分子的方法
公开(公告)号：US20120225431A1
公开(公告)日：2012-09-06
申请号：US13366769
申请日：2012-02-06
申请人： Jun E. Lee , Weidong Zheng
发明人： Jun E. Lee , Weidong Zheng
IPC分类号： C12Q1/68 , C07H1/00
CPC分类号： C07H21/04 , C07H1/00 , C07H21/02 , C12Q1/6813
摘要： The present invention is generally related to composition, kits and methods for synthesizing nucleic acid molecules and particularly for synthesizing labeled nucleic acid molecules. Specifically, the invention relates to methods, kits and compositions for synthesizing indirectly labeled nucleic acid molecules. The labeled nucleic acid molecules produced in accordance with the invention are particularly suited as labeled probes for nucleic acid detection, diagnostics, and array analysis.
摘要翻译：本发明通常涉及用于合成核酸分子，特别是用于合成标记的核酸分子的组合物，试剂盒和方法。具体地，本发明涉及用于合成间接标记的核酸分子的方法，试剂盒和组合物。根据本发明产生的标记的核酸分子特别适用于用于核酸检测，诊断和阵列分析的标记探针。

2. 发明授权

US09376698B2 Mutant DNA polymerases 有权
标题翻译：突变DNA聚合酶
公开(公告)号：US09376698B2
公开(公告)日：2016-06-28
申请号：US12052682
申请日：2008-03-20
申请人： Weidong Zheng , Jun E. Lee , Robert Jason Potter , David Mandelman
发明人： Weidong Zheng , Jun E. Lee , Robert Jason Potter , David Mandelman
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6806 , C12Q1/686 , C12Y207/07007 , Y02P20/52 , C12Q2561/101 , C12Q2527/149 , C12Q2521/101
摘要： An isolated mutant Tfi DNA polymerase having a D144A point mutation. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis.
摘要翻译：具有D144A点突变的分离的突变Tfi DNA聚合酶。该聚合酶可用于包括但不限于核酸合成，DNA测序，核酸扩增和cDNA合成的方法。

3. 发明申请

US20080254525A1 DNA Polymerase Blends and Mutant DNA Polymerases 有权
标题翻译： DNA聚合酶共混物和突变型DNA聚合酶
公开(公告)号：US20080254525A1
公开(公告)日：2008-10-16
申请号：US12052682
申请日：2008-03-20
申请人： Weidong Zheng , Jun E. Lee , Robert Jason Potter , David Mandelman
发明人： Weidong Zheng , Jun E. Lee , Robert Jason Potter , David Mandelman
IPC分类号： C12N9/10
CPC分类号： C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/6806 , C12Q1/686 , C12Y207/07007 , Y02P20/52 , C12Q2561/101 , C12Q2527/149 , C12Q2521/101
摘要： A thermostable DNA polymerase composition comprising at least two DNA polymerases, one of which is substantially reduced in 5′-exonuclease activity and one of which has 5′-exonuclease activity. This polymerase may be used in methods including, but not limited to, nucleic acid synthesis, DNA sequencing, nucleic acid amplification and cDNA synthesis,
摘要翻译：一种热稳定DNA聚合酶组合物，其包含至少两种DNA聚合酶，其中之一基本上降低了5'-核酸外切酶活性，其中一种具有5'-核酸外切酶活性。该聚合酶可用于包括但不限于核酸合成，DNA测序，核酸扩增和cDNA合成的方法，

4. 发明申请

US20110217735A1 COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) 有权
标题翻译：反向转录酶 - 聚合酶链反应的组合物和方法（RT-PCR）
公开(公告)号：US20110217735A1
公开(公告)日：2011-09-08
申请号：US12981240
申请日：2010-12-29
申请人： Jun E. Lee , Ayoub Rashtchian
发明人： Jun E. Lee , Ayoub Rashtchian
IPC分类号： C12P19/34 , C12N9/12
CPC分类号： C12P19/34 , C12N9/1276 , C12N15/1096 , C12Q1/6846 , C12Q2527/149 , C12Q2527/125 , C12Q2521/107
摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.
摘要翻译：本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法（或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

5. 发明授权

US09505846B2 Multi-component inhibitors of nucleic acid polymerases 有权
公开(公告)号：US09505846B2
公开(公告)日：2016-11-29
申请号：US11044620
申请日：2005-01-28
申请人： John D. Bishop , Jun E. Lee
发明人： John D. Bishop , Jun E. Lee
IPC分类号： C12P19/34 , C07K16/40 , C12Q1/68
CPC分类号： C07K16/40 , C07K2317/24 , C07K2317/76 , C12N9/93 , C12P19/34 , C12Q1/6848 , C12Q2527/127 , C12Q2521/119 , C12Q2521/101
摘要： The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity. The disclosed multi-component inhibitors are useful for DNA sequencing, nucleic acid amplification, cloning and synthesis, and the like.

6. 发明申请

US20100068767A1 MULTI-COMPONENT INHIBITORS OF NUCLEIC ACID POLYMERASES 审中-公开
标题翻译：核酸聚合物的多组分抑制剂
公开(公告)号：US20100068767A1
公开(公告)日：2010-03-18
申请号：US12541119
申请日：2009-08-13
申请人： John D. Bishop , Jun E. Lee
发明人： John D. Bishop , Jun E. Lee
IPC分类号： C12P19/34 , C12N9/10 , C12N9/08
CPC分类号： C07K16/40 , C07K2317/24 , C07K2317/76 , C12N9/93 , C12P19/34 , C12Q1/6848 , C12Q2527/127 , C12Q2521/119 , C12Q2521/101
摘要： The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity. The disclosed multi-component inhibitors are useful for DNA sequencing, nucleic acid amplification, cloning and synthesis, and the like.
摘要翻译：本发明提供核酸聚合酶的多组分抑制剂，制备方法及其使用方法。多组分抑制剂的一个组分是结合聚合酶（即，聚合酶结合分子（PBM））的分子，但不会基本上抑制其聚合酶活性。另一个组分是结合PBM（即PBM结合分子）的分子或分子复合物。 PBM和PBM结合分子/复合物的组合基本上抑制聚合酶活性。所公开的多组分抑制剂可用于DNA测序，核酸扩增，克隆和合成等。

7. 发明授权

US06495350B1 Compositions comprising a M-MLV reverse transcriptase and a DNA polymerase and use thereof 有权
标题翻译：包含M-MLV逆转录酶和DNA聚合酶的组合物及其用途
公开(公告)号：US06495350B1
公开(公告)日：2002-12-17
申请号：US09472066
申请日：1999-12-23
申请人： Jun E. Lee , Ayoub Rashtchian
发明人： Jun E. Lee , Ayoub Rashtchian
IPC分类号： C12Q168
CPC分类号： C12P19/34 , C12N9/1276 , C12N15/1096 , C12Q1/6846 , C12Q2527/149 , C12Q2527/125 , C12Q2521/107
摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.
摘要翻译：本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法（或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

8. 发明授权

US09637551B2 Multi-component inhibitors of nucleic acid polymerases 有权
公开(公告)号：US09637551B2
公开(公告)日：2017-05-02
申请号：US12636655
申请日：2009-12-11
申请人： John D. Bishop , Jun E. Lee
发明人： John D. Bishop , Jun E. Lee
IPC分类号： C12N9/12 , C07K16/40 , C12Q1/68
CPC分类号： C07K16/40 , C07K2317/24 , C07K2317/76 , C12N9/93 , C12P19/34 , C12Q1/6848 , C12Q2527/127 , C12Q2521/119 , C12Q2521/101
摘要： The present invention provides multi-component inhibitors of nucleic acid polymerases, methods of making, and methods of using same. One component of the multi-component inhibitor is a molecule that binds to a polymerase (i.e., a polymerase-binding molecule (PBM)), but does not thereby substantially inhibit its polymerase activity. Another component is a molecule or complex of molecules that binds to a PBM (i.e., a PBM-binding molecule). The combination of the PBM and PBM-binding molecule/complex substantially inhibits polymerase activity.

9. 发明授权

US07883871B2 Compositions and methods for reverse transcriptase-polymerase chain reaction (RT-PCR) 有权
标题翻译：逆转录聚合酶链反应（RT-PCR）的组合物和方法
公开(公告)号：US07883871B2
公开(公告)日：2011-02-08
申请号：US10861469
申请日：2004-06-07
申请人： Jun E. Lee , Ayoub Rashtchian
发明人： Jun E. Lee , Ayoub Rashtchian
IPC分类号： C12P19/34 , C07H21/04
CPC分类号： C12P19/34 , C12N9/1276 , C12N15/1096 , C12Q1/6846 , C12Q2527/149 , C12Q2527/125 , C12Q2521/107
摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.
摘要翻译：本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法（或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

10. 发明申请

US20100075382A1 SSB-POLYMERASE FUSION PROTEINS 有权
标题翻译： SSB聚合酶融合蛋白
公开(公告)号：US20100075382A1
公开(公告)日：2010-03-25
申请号：US12326048
申请日：2008-12-01
申请人： Jun E. Lee , Robert J. Potter , David Mandelman
发明人： Jun E. Lee , Robert J. Potter , David Mandelman
IPC分类号： C12P19/34 , C12N9/12 , C12N1/00 , C12N15/63 , C07H21/04
CPC分类号： C12Q1/6806 , C12N9/1252 , C12N9/1276 , C12N15/62 , C12Q1/6848 , C12Q2522/101
摘要： Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
摘要翻译：包含单链DNA结合蛋白和核酸聚合酶（例如DNA聚合酶或逆转录酶）的融合蛋白。这些高保真蛋白质适用于核酸扩增方法，包括聚合酶链反应（PCR）。

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