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1. 发明授权

US06300082B1 Method and apparatus for measurement of the effect of test compounds on signal transduction at the level of biological receptors 失效
公开(公告)号：US06300082B1
公开(公告)日：2001-10-09
申请号：US09045224
申请日：1998-03-20
申请人： Judith L. Erb , James G. Downward, IV , John R. Erb-Downward , James L. Wittliff
发明人： Judith L. Erb , James G. Downward, IV , John R. Erb-Downward , James L. Wittliff
IPC分类号： G01N33543
CPC分类号： G01N21/648 , G01N21/7703 , Y10S435/808 , Y10S436/805 , Y10S436/811 , Y10S436/813 , Y10S436/814 , Y10S436/815 , Y10S436/817
摘要： A method and apparatus for measuring binding between a plurality of molecules of a biological receptor protein and a plurality of molecules of a type which binds to said biological receptor is presented. Apparatus utilizes a sensor possessing a waveguide to which have been attached in close proximity to its surface, features resembling molecules having binding affinity for said biological receptor. Light is injected into said waveguide so as to produce an evanescent field at its surface. Molecules of receptor protein are tagged with a tag belonging to that class of chemicals which alters a characteristic of light, when said light passes through said chemical tag. Apparatus utilizes a rapid means of monitoring the change in optical signal coming from the waveguide as binding proceeds between tagged receptor protein and the binding molecular feature of the waveguide. This allows direct measurement of binding and dissociation rates between the receptor and the binding feature of the waveguide. By using a waveguide having a feature resembling a ligand for the receptor, the potential hormonal activity of a test substance may be evaluated from its ability to compete with the waveguide for binding with the receptor. The effect of a test compound on binding of receptor protein to a subsequent element in a hormonal signal transduction mechanism is evaluated by measuring the impact of the test compound on binding between receptor protein and a feature resembling said next element of the signal transduction mechanism. Methods are provided whereby such data may be used to compute affinity constants, binding activity, complex affinity constants resulting from cooperativity, and kinetic parameters for the receptor and test ligand and for the receptor and the next element of the signal transduction mechanism. Preferred embodiments of the invention illustrate application of the method and apparatus to measuring binding between biological receptors and their nuclear response elements, and the use of this type of measurement for assessment of the activity of estrogen mimics present in a test sample, and for evaluation of pharmaceuticals intended to treat hormone dependent cancers.

2. 发明申请

US20110195995A1 Methods of Optimizing Treatment of Estrogen-Receptor Positive Breast Cancers 审中-公开
标题翻译：优化雌激素受体阳性乳腺癌治疗方法
公开(公告)号：US20110195995A1
公开(公告)日：2011-08-11
申请号：US13123158
申请日：2009-10-13
申请人： James L. Wittliff , Daniel Allan Kerr, II
发明人： James L. Wittliff , Daniel Allan Kerr, II
IPC分类号： A61K31/4535 , C12Q1/68 , A61K31/135 , A61P35/00
CPC分类号： G01N33/57415 , C12Q1/6886 , C12Q2600/106 , C12Q2600/118 , G01N2800/52
摘要： Estrogen-receptor positive and progesterone-receptor positive breast cancer treatment can be optimized by determining the level of expression of genes in a breast sample from a human having an estrogen-receptor positive and progesterone-receptor positive breast cancer that identify a human with an increased likelihood of recurrence of the breast cancer.
摘要翻译：雌激素受体阳性和孕激素受体阳性乳腺癌治疗可以通过确定来自具有雌激素受体阳性和孕激素受体阳性乳腺癌的人的乳腺样品中基因表达的水平来鉴定具有增加的人乳腺癌复发的可能性。

3. 发明申请

US20110065115A1 METHODS FOR IDENTIFYING AN INCREASED LIKELIHOOD OF RECURRENCE OF BREAST CANCER 审中-公开
标题翻译：识别乳腺癌复发性增生症的方法
公开(公告)号：US20110065115A1
公开(公告)日：2011-03-17
申请号：US12885720
申请日：2010-09-20
申请人： James L. Wittliff , Sarah A. Andres
发明人： James L. Wittliff , Sarah A. Andres
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/118 , C12Q2600/158
摘要： Methods of identifying a mammal having an increased likelihood of recurrence of breast cancer includes identifying in a breast tissue sample of the mammal expression of at least two genes selected from the group consisting of Hs.125867 (EVL), Hs.591847 (NAT1), Hs.208124 (ESR1), Hs.26225 (GABRP), Hs.408614 (ST8SIA1), Hs.480819 (TBC1D9), Hs.504115 (TRIM29), Hs.523468 (SCUBE2), Hs.532082 (IL6ST), Hs.592121 (RABEP1), Hs.79136 (SLC39A6), Hs.82128 (TPBG), Hs.95243 (TCEAL1), Hs.95612 (DSC2), Hs.654961 (FUT8), Hs.1594 (CENPA), Hs.184339 (MELK), Hs.26010 (PFKP), Hs.592049 (PLK1), Hs.370834 (ATAD2), Hs.437638 (XBP1), Hs.444118 (MCM6), Hs.469649 (BUB1), Hs.470477 (PTP4A2), Hs.473583 (YBX1), Hs.480938 (LRBA), Hs.524134 (GATA3), Hs.531668 (CX3CL1), Hs.532824 (MAPRE2), Hs.591314 (GMPS), Hs.83758 (CKS2) and Hs.99962 (SLC43A3) and subsets of the genes.
摘要翻译：鉴定具有增加的乳腺癌复发可能性的哺乳动物的方法包括在乳腺组织样品中鉴定哺乳动物表达中至少两种选自Hs.125867（EVL），Hs.591847（NAT1）， Hs.208124（ESR1），Hs.26225（GABRP），Hs.408614（ST8SIA1），Hs.480819（TBC1D9），Hs.504115（TRIM29），Hs.523468（SCUBE2），Hs.532082（IL6ST），Hs H9.79136（SLC39A6），Hs.82128（TPBG），Hs.95243（TCEAL1），Hs.95612（DSC2），Hs.654961（FUT8），Hs.1594（CENPA），Hs。 184339（MELK），Hs.26010（PFKP），Hs.592049（PLK1），Hs.370834（ATAD2），Hs.437638（XBP1），Hs.444118（MCM6），Hs.469649（BUB1），Hs.470477 （PTP4A2），Hs.473583（YBX1），Hs.480938（LRBA），Hs.524134（GATA3），Hs.531668（CX3CL1），Hs.532824（MAPRE2），Hs.591314（GMPS），Hs.83758（ CKS2）和Hs.99962（SLC43A3）和基因的子集。

4. 发明申请

US20110165566A1 METHODS OF OPTIMIZING TREATMENT OF BREAST CANCER 审中-公开
标题翻译：优化乳腺癌治疗方法
公开(公告)号：US20110165566A1
公开(公告)日：2011-07-07
申请号：US12832474
申请日：2010-07-08
申请人： James L. Wittliff , Sarah A. Andres
发明人： James L. Wittliff , Sarah A. Andres
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/118 , C12Q2600/158
摘要： Breast cancer treatment can be optimized by determining the level of expression of genes in a breast sample from a human to identify a human with an increased likelihood of recurrence of the breast cancer that would potentially benefit from a therapy to decrease the likelihood of recurrence of the breast cancer.
摘要翻译：可以通过确定来自人的乳腺样品中基因表达的水平来确定乳腺癌治疗，以鉴定具有增加的乳腺癌复发可能性的人，其可能从治疗中受益以减少复发的可能性乳腺癌。

5. 发明申请

US20100112592A1 METHODS FOR IDENTIFYING AN INCREASED LIKELIHOOD OF RECURRENCE OF BREAST CANCER 审中-公开
标题翻译：识别乳腺癌复发性增生症的方法
公开(公告)号：US20100112592A1
公开(公告)日：2010-05-06
申请号：US12630212
申请日：2009-12-03
申请人： James L. Wittliff , Sarah A. Andres
发明人： James L. Wittliff , Sarah A. Andres
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q2600/112 , C12Q2600/118 , C12Q2600/158
摘要： Methods of identifying a mammal having an increased likelihood of recurrence of breast cancer includes identifying in a breast tissue sample of the mammal expression of at least two genes selected from the group consisting of Hs.125867 (EVL), Hs.591847 (NAT1), Hs.208124 (ESR1), Hs.26225 (GABRP), Hs.408614 (ST8SIA1), Hs.480819 (TBC1D9), Hs.504115 (TRIM29), Hs.523468 (SCUBE2), Hs.532082 (IL6ST), Hs.592121 (RABEP1), Hs.79136 (SLC39A6), Hs.82128 (TPBG), Hs.95243 (TCEAL1), Hs.95612 (DSC2), Hs.654961 (FUT8), Hs.1594 (CENPA), Hs.184339 (MELK), Hs.26010 (PFKP), Hs.592049 (PLK1), Hs.370834 (ATAD2), Hs.437638 (XBP1), Hs.444118 (MCM6), Hs.469649 (BUB1), Hs.470477 (PTP4A2), Hs.473583 (YBX1), Hs.480938 (LRBA), Hs.524134 (GATA3), Hs.531668 (CX3CL1), Hs.532824 (MAPRE2), Hs.591314 (GMPS), Hs.83758 (CKS2) and Hs.99962 (SLC43A3) and subsets of the genes.
摘要翻译：鉴定具有增加的乳腺癌复发可能性的哺乳动物的方法包括在乳腺组织样品中鉴定哺乳动物表达中至少两种选自Hs.125867（EVL），Hs.591847（NAT1）， Hs.208124（ESR1），Hs.26225（GABRP），Hs.408614（ST8SIA1），Hs.480819（TBC1D9），Hs.504115（TRIM29），Hs.523468（SCUBE2），Hs.532082（IL6ST），Hs H9.79136（SLC39A6），Hs.82128（TPBG），Hs.95243（TCEAL1），Hs.95612（DSC2），Hs.654961（FUT8），Hs.1594（CENPA），Hs。 184339（MELK），Hs.26010（PFKP），Hs.592049（PLK1），Hs.370834（ATAD2），Hs.437638（XBP1），Hs.444118（MCM6），Hs.469649（BUB1），Hs.470477 （PTP4A2），Hs.473583（YBX1），Hs.480938（LRBA），Hs.524134（GATA3），Hs.531668（CX3CL1），Hs.532824（MAPRE2），Hs.591314（GMPS），Hs.83758（ CKS2）和Hs.99962（SLC43A3）和基因的子集。

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