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1. 发明申请

US20140220623A1 METHODS AND COMPOSITIONS FOR ESTIMATING SOIL MICROBIAL LOAD 有权
标题翻译：估算土壤微生物负荷的方法和组合
公开(公告)号：US20140220623A1
公开(公告)日：2014-08-07
申请号：US14242652
申请日：2014-04-01
申请人： Judith Fitzpatrick , James Sottilo , Austin Lin , Brady Trexler
发明人： Judith Fitzpatrick , James Sottilo , Austin Lin , Brady Trexler
IPC分类号： C12Q1/06
CPC分类号： C12Q1/06 , C12Q1/24 , G01N15/06 , G01N2015/0061 , G01N2015/0693
摘要： Methods and compositions for the preparation of soil samples and the determination of the number of microbes within a soil sample are disclosed. The methods include measuring, solubilizing, bleaching and filtering soil samples and measuring the turbidity of the filtered solution. The turbidity of the sample can be determined by visual inspection of a Secchi disk viewed through the column liquid sample in a transparent tubular cylinder, or by using a cell phone camera and application. Devices and kits for the rapid, cost efficient determination of microbial numbers in the field setting are disclosed. The disclosed devices and methods can also be used for applications other than determination of microbial numbers.
摘要翻译：公开了土壤样品制备方法和组合物以及土壤样品中微生物数量的确定。这些方法包括测量，溶解，漂白和过滤土壤样品并测量过滤溶液的浊度。样品的浊度可以通过目视检查通过透明管状圆筒中的柱状液体样品观察到的Secchi圆盘，或者通过使用手机相机和应用来确定。公开了用于在现场设置中快速，成本有效地测定微生物数量的装置和试剂盒。所公开的装置和方法也可以用于除了确定微生物数量之外的应用。

2. 发明授权

US06465655B1 Antibody to and assay for peptide linked-pyridinoline as indicator of bone resorption level 有权
标题翻译：肽连接 - 吡啶啉的抗体和测定作为骨吸收水平的指标
公开(公告)号：US06465655B1
公开(公告)日：2002-10-15
申请号：US09444162
申请日：1999-11-22
申请人： Judith Fitzpatrick , Regina Lenda , Zijian Zeng
发明人： Judith Fitzpatrick , Regina Lenda , Zijian Zeng
IPC分类号： C07D21172
CPC分类号： C07K16/18 , G01N33/5308 , G01N33/68 , Y10S436/815 , Y10S530/809
摘要： A monoclonal antibody, Serex A93, recognizes peptide linked pyridinoline in Type I bone collagen fragments and can be used to quantitate bone resorption. The hybridoma producing this antibody has been deposited with the American Type Culture Collection, Rockville, Md., under accession number HB-12254. The epitope reactive with the antibody is stable to acid hydrolysis and therefore is not a linear peptide. The antibody recognizes pyridinoline in bound or conjugated form, but not in the free form found in urine. The antibody is useful in immunoassays for determining bone resorption, utilizing body fluids such as urine, saliva, blood, or sweat.
摘要翻译：单克隆抗体Serex A93识别I型骨胶原片段中的肽连接的吡啶啉，可用于定量骨吸收。产生该抗体的杂交瘤已经保藏在美国典型培养物保藏中心，Rockville，Md。，保藏号为HB-12254。与抗体反应的表位对酸水解是稳定的，因此不是线性肽。抗体识别结合或缀合形式的吡啶啉，但不能以尿液中的游离形式存在。该抗体可用于免疫测定以确定骨吸收，利用体液如尿液，唾液，血液或汗液。

3. 发明授权

US5710009A Receptor:release ligand (reland) complexes and release assays using said reland and methods and kits based thereon 失效
标题翻译：受体：释放配体（reland）复合物和释放测定，使用所述相关性和基于其的方法和试剂盒
公开(公告)号：US5710009A
公开(公告)日：1998-01-20
申请号：US493420
申请日：1995-06-22
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： G01N33/531 , G01N33/536 , G01N33/543 , G01N33/58 , G01N33/72 , G01N33/94 , G01N33/535
CPC分类号： G01N33/721 , G01N33/536 , G01N33/58 , G01N33/94 , Y10S435/975 , Y10S436/808
摘要： Assays using receptor:reland complexes capable of releasing the reland in the presence of an analyte are described, wherein the reland does not detectably compete with analyte for binding to the receptor. The dissociation constant of the reland and the receptor is such that no appreciable release of reland occurs in the absence of analyte for the receptor. In a preferred embodiment, the association constant of the monomeric reland and the receptor is less than or equal to about 10.sup.5 M, preferably 10.sup.3 to 10.sup.5 M, most preferably 1% or less of the association constant of the analyte and receptor. In a preferred embodiment, the reland is labelled and the amount of analyte bound to the receptor is determined from the amount of labelled reland which is released.
摘要翻译：描述了使用能够在分析物存在下释放相关物的受体：相对配合物的测定法，其中所述依赖物不可检测地与分析物竞争结合受体。相关物和受体的解离常数使得在不存在受体的分析物的情况下不会发生明显的释放释放。在优选的实施方案中，单体相对和受体的缔合常数小于或等于分析物和受体的缔合常数的约105M，优选103至105M，最优选1％或更小。在一个优选的实施方案中，依赖物被标记，并且结合于受体的分析物的量由被释放的标记的依赖性的量确定。

4. 发明授权

US06808889B2 Method and device for detection of Apo A, Apo B and the ratio thereof in saliva 失效
标题翻译：用于检测Apo A，Apo B及其在唾液中的比例的方法和装置
公开(公告)号：US06808889B2
公开(公告)日：2004-10-26
申请号：US09526582
申请日：2000-03-16
申请人： Judith Fitzpatrick , Regina B. Lenda , Christopher L. Jones
发明人： Judith Fitzpatrick , Regina B. Lenda , Christopher L. Jones
IPC分类号： G01N3353
CPC分类号： G01N33/92 , G01N2333/775 , G01N2800/044 , Y10S435/81 , Y10S436/808 , Y10T436/25
摘要： A method has been developed to detect the levels of apolipoproteins A-1 and B in saliva, which is correlated with the levels of HDL and LDL in serum, respectively. In unstimulated saliva, the ration of Apo A to Apo B is correlated with the ration of HDL to LDL in serum. Albumin can be used to normalize the sample for dilution. The high degree of correlation in combination with a simple, quick test that can be performed at the site of collection provides a cost effective, patient friendly means to monitor an individual's risk of heart disease. In the preferred embodiment, saliva production is stimulated by means such as breath mint or tart solution (such as lemon) and the effect of dilution controlled by reference to albumin.
摘要翻译：已经开发了一种检测唾液中载脂蛋白A-1和B水平的方法，分别与血清中的HDL和LDL水平相关。在未刺激的唾液中，Apo A对Apo B的配对与血清中HDL与LDL的比例相关。白蛋白可用于标准化样品进行稀释。结合高度相关性，可以在收集现场进行的简单，快速的检测提供了一种成本效益高，耐心的手段来监测个体的心脏病风险。在优选的实施方案中，通过诸如呼吸薄荷或挞溶液（例如柠檬）的手段刺激唾液产生，并通过参照白蛋白控制稀释效果。

5. 发明授权

US6087185A Integrated packaging holder device for immunochromatographic assays in flow-through or dipstick formats 失效
标题翻译：集成包装支架装置，用于流通或定尺格式的免疫色谱测定
公开(公告)号：US6087185A
公开(公告)日：2000-07-11
申请号：US971250
申请日：1997-11-17
申请人： F. Victor Lee-Own , Judith Fitzpatrick
发明人： F. Victor Lee-Own , Judith Fitzpatrick
IPC分类号： G01N33/543 , G01N33/537 , G01N33/558 , G01N37/00
CPC分类号： G01N33/558 , Y10S435/97
摘要： The prevent invention relates to an integrated package-holder assay devices for detecting the presence of analyte in a sample. The device serves the dual roles of supporting and protecting an immunochromatographic assay. The device is compatible with any immunochromatographic assay format. The assay can be performed in a single apparatus for use in a laboratory or a field setting. In a specific example, the assay device is a nylon membrane formatted for an immunochromatographic assay for cotinine sealed between transparent adhesive tape and a stiff plastic strip. White tape placed over the plastic strip defined a window for observing the assay results.
摘要翻译：本发明涉及用于检测样品中分析物的存在的集成的包装盒测定装置。该装置起支持和保护免疫色谱测定的双重作用。该装置与任何免疫色谱测定格式兼容。该测定可以在用于实验室或场设置的单个装置中进行。在具体实例中，测定装置是格式化用于免疫色谱测定的尼龙膜，用于可密切粘合在透明胶带和硬塑料条之间的可替宁。置于塑料条上的白色胶带定义了一个用于观察测定结果的窗口。

6. 发明授权

US5989925A Antibody to and assay for peptide linked-pyridinoline as indicator of bone resorption level 失效
公开(公告)号：US5989925A
公开(公告)日：1999-11-23
申请号：US884148
申请日：1997-06-27
申请人： Judith Fitzpatrick , Regina Lenda , Zijian Zeng
发明人： Judith Fitzpatrick , Regina Lenda , Zijian Zeng
IPC分类号： C07K16/18 , G01N33/53 , G01N33/68 , G01N33/553 , C07K16/81
CPC分类号： C07K16/18 , G01N33/5308 , G01N33/68 , Y10S436/815 , Y10S530/809
摘要： A monoclonal antibody, Serex A93, recognizes peptide linked pyridinoline in Type I bone collagen fragments and can be used to quantitate bone resorption. The hybridoma producing this antibody has been deposited with the American Type Culture Collection, Rockville, Md., under accession number HB-12254. The epitope reactive with the antibody is stable to acid hydrolysis and therefore is not a linear peptide. The antibody recognizes pyridinoline in bound or conjugated form, but not in the free form found in urine. The antibody is useful in immunoassays for determining bone resorption, utilizing body fluids such as urine, saliva, blood, or sweat.

7. 发明授权

US07335516B2 Methods to improve immunogenicity of antigens and specificity of antibodies 失效
标题翻译：提高抗原免疫原性和抗体特异性的方法
公开(公告)号：US07335516B2
公开(公告)日：2008-02-26
申请号：US10224772
申请日：2002-08-21
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： G01N33/531 , A61K38/03 , C07K17/02 , C07K16/44
CPC分类号： C07K16/16 , A61K39/385 , A61K47/646 , A61K2039/6081 , A61K2039/627 , C07K16/18
摘要： A method of improving specific immune responses to small immunogens, haptens, has been developed by changing the linkage between the hapten and carrier being used for immunization. Antibodies to a glycated protein have been developed, utilizing an immunogen which is composed of a glycated peptide mimic of the glycated peptide sequence which is the target epitope, wherein the peptide mimic is constructed to conformationally mimic the conformation of the peptide in the native protein, the peptide mimic contains no charged groups or other immunodominant group, and the peptide mimic is connected to a spacer sequence equivalent to a peptide spacer of between one and thirty amino acids in length, which serves to position the peptide epitope in a conformation that approximates its conformation in the native protein. In a further embodiment the peptide mimic and spacer are linked to a carrier molecule.
摘要翻译：已经通过改变用于免疫的半抗原和载体之间的连接来开发提高对小免疫原（haptens）的特异性免疫应答的方法。已经开发出抗糖基化蛋白质的抗体，利用由作为靶表位的糖基化肽序列的糖基化肽模拟物构成的免疫原，其中肽模拟物构建成构象地模拟天然蛋白质中肽的构象，肽模拟物不含有带电荷基团或其他免疫显性基团，并且肽模拟物连接至长度为一至三十个氨基酸的等同于肽间隔基的间隔序列，其用于将肽表位置于近似于其的构象天然蛋白质中的构象。在另一个实施方案中，肽模拟物和间隔基连接到载体分子。

8. 发明授权

US5527686A Differential binding affinities and dissociation assays based thereon 失效
标题翻译：基于此的差异结合亲和力和解离测定
公开(公告)号：US5527686A
公开(公告)日：1996-06-18
申请号：US196092
申请日：1994-02-17
申请人： Judith Fitzpatrick , Regina Lenda
发明人： Judith Fitzpatrick , Regina Lenda
IPC分类号： G01N33/53 , G01N33/58 , G01N33/72 , G01N33/94 , G01N33/535
CPC分类号： G01N33/58 , G01N33/53 , G01N33/721 , G01N33/94 , Y10S435/975 , Y10S436/815
摘要： A method for assaying for the presence of analyte in a sample based on differential binding affinity involves detecting dissociation of a complex of receptor and ligand in the presence of analyte. The receptor binds the analyte with high affinity and with the ligand with low affinity. The receptor-ligand complex may be formed in situ or may be preformed. In the presence of free analyte, the receptor releases from the receptor-ligand complex and binds free analyte. Release of the receptor-ligand complex is detectable. A kit for performing release assays to detect the presence of analyte is also provided.
摘要翻译： PCT No.PCT / US92 / 06249 Sec。 371日期1994年2月17日 102（e）日期1994年2月17日PCT提交1992年7月29日PCT公布。公开号WO93 / 03367 日期：1993年2月18日。基于差异结合亲和力测定样品中分析物的存在的方法包括检测在分析物存在下受体和配体的复合物的解离。受体以高亲和力和低亲和力的配体结合分析物。受体 - 配体复合物可以原位形成或可以预先形成。在游离分析物的存在下，受体从受体 - 配体复合物释放并结合游离的分析物。受体 - 配体复合物的释放是可检测的。还提供了用于进行释放测定以检测分析物存在的试剂盒。

9. 发明授权

US5451504A Method and device for detecting the presence of analyte in a sample 失效
标题翻译：用于检测样品中分析物的存在的方法和装置
公开(公告)号：US5451504A
公开(公告)日：1995-09-19
申请号：US737091
申请日：1991-07-29
申请人： Judith Fitzpatrick , Regina B. Lenda
发明人： Judith Fitzpatrick , Regina B. Lenda
IPC分类号： G01N33/558 , G01N33/53 , G01N33/537 , G01N33/543
CPC分类号： G01N33/558 , Y10S435/805 , Y10S435/971 , Y10S435/973
摘要： An assay provides for detecting the presence of an analyte in a sample. Sample is applied to move through three zones. A mobilizeable receptor capable of binding to the analyte is present in the first zone. A trap for unbound receptor, consisting of immobilized ligand, is present in the second zone. Receptor bound to analyte will not bind to the ligand; unbound receptor will bind to the ligand. Mobilization and migration of the receptor can be detected in the third zone, which positively indicates that analyte is present. A device for carrying out the method is also provided.
摘要翻译：检测法用于检测样品中分析物的存在。应用样本移动通过三个区域。能够结合分析物的可移动受体存在于第一区域中。在第二区域中存在由固定化配体组成的未结合受体的陷阱。与分析物结合的受体不会与配体结合; 未结合的受体将结合配体。可以在第三区域中检测到受体的动员和迁移，这表明存在分析物。还提供了一种用于执行该方法的装置。

10. 发明授权

US07259232B1 Preferred segments of neural thread protein 有权
标题翻译：神经线蛋白的优选区段
公开(公告)号：US07259232B1
公开(公告)日：2007-08-21
申请号：US09697590
申请日：2000-10-27
申请人： Judith Fitzpatrick , Paul Averback , Maggie Focht , Riza Bibiano
发明人： Judith Fitzpatrick , Paul Averback , Maggie Focht , Riza Bibiano
IPC分类号： C07K14/435 , C07K5/04
CPC分类号： C07K14/4711 , A61K38/00 , C07K2319/00
摘要： The invention is directed to preferred repeat sequences of Neural Thread Protein (NTP), peptides, mimetics, antibodies, and nucleic acids of the preferred sequences, and diagnostic and therapeutic methods of using such preferred NTP sequences.
摘要翻译：本发明涉及优选的重复序列的神经螺蛋白（NTP），肽，模拟物，抗体和优选序列的核酸，以及使用优选的NTP序列的诊断和治疗方法。

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