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1. 发明申请

US20190271661A1 Enhanced Isotachophoresis Assays Using Additives with Spatial Gradients 审中-公开
公开(公告)号：US20190271661A1
公开(公告)日：2019-09-05
申请号：US16193535
申请日：2018-11-16
申请人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
发明人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
IPC分类号： G01N27/447
摘要： Techniques for enhanced isotachophoresis assays using additives with spatial gradients include forming a concentration gradient of an additive along a channel from an input port to an output port. The channel is used for isotachophoresis with ions of a leading electrolyte having a first mobility greater than a mobility of an analyte, and ions of a trailing electrolyte having a second mobility less than the mobility of the analyte. The additive is different from both the leading electrolyte and the trailing electrolyte; and the additive has a third mobility that assures the analyte will encounter the additive. The method further comprises introducing a mixture of the trailing electrolyte and a sample including the analyte. The method further comprises applying an electric field to the channel; and, measuring the analyte.

2. 发明授权

US10132775B2 Enhanced isotachophoresis assays using additives with spatial gradients 有权
公开(公告)号：US10132775B2
公开(公告)日：2018-11-20
申请号：US14828487
申请日：2015-08-17
申请人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
发明人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
IPC分类号： G01N27/447
摘要： Techniques for enhanced isotachophoresis assays using additives with spatial gradients include forming a concentration gradient of an additive along a channel from an input port to an output port. The channel is used for isotachophoresis with ions of a leading electrolyte having a first mobility greater than a mobility of an analyte, and ions of a trailing electrolyte having a second mobility less than the mobility of the analyte. The additive is different from both the leading electrolyte and the trailing electrolyte; and the additive has a third mobility that assures the analyte will encounter the additive. The method further comprises introducing a mixture of the trailing electrolyte and a sample including the analyte. The method further comprises applying an electric field to the channel; and, measuring the analyte.

3. 发明申请

US20160153934A1 Enhanced Isotachophoresis Assays Using Additives with Spatial Gradients 审中-公开
标题翻译：使用添加剂与空间梯度增强等速电泳分析
公开(公告)号：US20160153934A1
公开(公告)日：2016-06-02
申请号：US14828487
申请日：2015-08-17
申请人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
发明人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
IPC分类号： G01N27/447
CPC分类号： G01N27/44756 , G01N27/44721 , G01N27/4473 , G01N27/44795
摘要： Techniques for enhanced isotachophoresis assays using additives with spatial gradients include forming a concentration gradient of an additive along a channel from an input port to an output port. The channel is used for isotachophoresis with ions of a leading electrolyte having a first mobility greater than a mobility of an analyte, and ions of a trailing electrolyte having a second mobility less than the mobility of the analyte. The additive is different from both the leading electrolyte and the trailing electrolyte; and the additive has a third mobility that assures the analyte will encounter the additive. The method further comprises introducing a mixture of the trailing electrolyte and a sample including the analyte. The method further comprises applying an electric field to the channel; and, measuring the analyte.
摘要翻译：使用具有空间梯度的添加剂进行增强等速电泳测定的技术包括沿着通道从输入端口到输出端口形成添加剂的浓度梯度。该通道用于具有第一迁移率大于分析物的迁移率的前导电解质的离子和具有小于分析物的迁移率的第二迁移率的尾电解质的离子的等速电泳。添加剂不同于主要电解质和尾电解质; 并且添加剂具有确保分析物将遇到添加剂的第三迁移率。该方法还包括引入拖尾电解质和包括分析物的样品的混合物。该方法还包括向信道施加电场; 并测量分析物。

4. 发明授权

US09151732B2 Enhanced isotachophoresis assays using additives with spatial gradients 有权
标题翻译：使用具有空间梯度的添加剂的增强的等速电泳测定
公开(公告)号：US09151732B2
公开(公告)日：2015-10-06
申请号：US13252138
申请日：2011-10-03
申请人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
发明人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
IPC分类号： G01N27/447
CPC分类号： G01N27/44756 , G01N27/44721 , G01N27/4473 , G01N27/44795
摘要： Techniques for enhanced isotachophoresis assays using additives with spatial gradients include forming a concentration gradient of an additive along a channel from an input port to an output port. The channel is used for isotachophoresis with ions of a leading electrolyte having a first mobility greater than a mobility of an analyte, and ions of a trailing electrolyte having a second mobility less than the mobility of the analyte. The additive is different from both the leading electrolyte and the trailing electrolyte; and the additive has a third mobility that assures the analyte will encounter the additive. The method further comprises introducing a mixture of the trailing electrolyte and a sample including the analyte. The method further comprises applying an electric field to the channel; and, measuring the analyte.
摘要翻译：使用具有空间梯度的添加剂进行增强等速电泳测定的技术包括沿着通道从输入端口到输出端口形成添加剂的浓度梯度。该通道用于具有第一迁移率大于分析物的迁移率的前导电解质的离子和具有小于分析物的迁移率的第二迁移率的尾电解质的离子的等速电泳。添加剂不同于主要电解质和尾电解质; 并且添加剂具有确保分析物将遇到添加剂的第三迁移率。该方法还包括引入拖尾电解质和包括分析物的样品的混合物。该方法还包括向信道施加电场; 并测量分析物。

5. 发明申请

US20140014515A1 Enhanced Isotachophoresis Assays Using Additives with Spatial Gradients 有权
标题翻译：使用添加剂与空间梯度增强等速电泳分析
公开(公告)号：US20140014515A1
公开(公告)日：2014-01-16
申请号：US13252138
申请日：2011-10-03
申请人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
发明人： Juan G. Santiago , Alexandre Persat , Giancarlo Garcia , Charbel Eid
IPC分类号： G01N27/447 , G01N27/453
CPC分类号： G01N27/44756 , G01N27/44721 , G01N27/4473 , G01N27/44795
摘要： Techniques for enhanced isotachophoresis assays using additives with spatial gradients include forming a concentration gradient of an additive along a channel from an input port to an output port. The channel is used for isotachophoresis with ions of a leading electrolyte having a first mobility greater than a mobility of an analyte, and ions of a trailing electrolyte having a second mobility less than the mobility of the analyte. The additive is different from both the leading electrolyte and the trailing electrolyte; and the additive has a third mobility that assures the analyte will encounter the additive. The method further comprises introducing a mixture of the trailing electrolyte and a sample including the analyte. The method further comprises applying an electric field to the channel; and, measuring the analyte.
摘要翻译：使用具有空间梯度的添加剂进行增强等速电泳测定的技术包括沿着通道从输入端口到输出端口形成添加剂的浓度梯度。该通道用于具有第一迁移率大于分析物的迁移率的前导电解质的离子和具有小于分析物的迁移率的第二迁移率的尾电解质的离子的等速电泳。添加剂不同于前导电解质和尾电解质; 并且添加剂具有确保分析物将遇到添加剂的第三迁移率。该方法还包括引入拖尾电解质和包括分析物的样品的混合物。该方法还包括向信道施加电场; 并测量分析物。

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