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1. 发明申请

US20110118333A1 Use on Minicircle vectors for cardiac gene therapy 有权
标题翻译：在微循环载体上使用心脏基因治疗
公开(公告)号：US20110118333A1
公开(公告)日：2011-05-19
申请号：US12925483
申请日：2010-10-22
申请人： Joseph Wu , Mark A. Kay , Mei Huang , Zhi-Ying Chen
发明人： Joseph Wu , Mark A. Kay , Mei Huang , Zhi-Ying Chen
IPC分类号： A61K48/00 , C12N15/63 , A61P9/10
CPC分类号： C12N15/85 , C12N15/1137 , C12N2310/11 , C12N2330/51 , C12N2800/24 , C12Y114/11002 , C12N2310/14 , C12N2310/531
摘要： Compositions and methods are provided for the treatment of an ischemic cardiovascular condition by providing a patient with a novel non-viral minicircle DNA vector comprising polynucleotide sequences that potentiate HIF-1 activity, including RNAi or antisense agents selective for proteins involved in HIF1 inactivation.
摘要翻译：提供组合物和方法用于通过向患者提供包含增强HIF-1活性的多核苷酸序列的新型非病毒小环DNA载体，包括对涉及HIF1失活的蛋白质有选择性的RNAi或反义试剂来治疗缺血性心血管疾病。

2. 发明授权

US08445454B2 Use on minicircle vectors for cardiac gene therapy 有权
标题翻译：使用小环向量进行心脏基因治疗
公开(公告)号：US08445454B2
公开(公告)日：2013-05-21
申请号：US12925483
申请日：2010-10-22
申请人： Joseph Wu , Mark A. Kay , Mei Huang , Zhi-Ying Chen
发明人： Joseph Wu , Mark A. Kay , Mei Huang , Zhi-Ying Chen
IPC分类号： A61K48/00 , C07H21/04 , C07H21/02 , C12N15/63
CPC分类号： C12N15/85 , C12N15/1137 , C12N2310/11 , C12N2330/51 , C12N2800/24 , C12Y114/11002 , C12N2310/14 , C12N2310/531
摘要： Compositions and methods are provided for the treatment of an ischemic cardiovascular condition by providing a patient with a novel non-viral minicircle DNA vector comprising polynucleotide sequences that potentiate HIF-1 activity, including RNAi or antisense agents selective for proteins involved in HIF1 inactivation.
摘要翻译：提供组合物和方法用于通过向患者提供包含增强HIF-1活性的多核苷酸序列的新型非病毒小环DNA载体，包括对涉及HIF1失活的蛋白质有选择性的RNAi或反义试剂来治疗缺血性心血管病症。

3. 发明授权

US08962331B2 Method of making induced pluripotent stem cell from adipose stem cells using minicircle DNA vectors 有权
标题翻译：使用小环DNA载体从脂肪干细胞诱导多能干细胞的方法
公开(公告)号：US08962331B2
公开(公告)日：2015-02-24
申请号：US12931476
申请日：2011-02-01
申请人： Joseph Wu , Michael T. Longaker , Mark A. Kay , Ning Sung , FangJun Jia , Zhi-Ying Chen , Nicholas Panetta , Deepak Gupta
发明人： Joseph Wu , Michael T. Longaker , Mark A. Kay , Ning Sung , FangJun Jia , Zhi-Ying Chen , Nicholas Panetta , Deepak Gupta
IPC分类号： C12N15/00 , C12N5/00
CPC分类号： C12N5/0696 , C12N15/85 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2506/1384 , C12N2510/00 , C12N2800/00 , C12N2800/108 , C12N2800/24
摘要： Human somatic cells are reprogrammed to become induced pluripotent stem cells (iPS cells) by the introduction of a minicircle DNA vector. Cells of interest include adipose stem cells.
摘要翻译：通过引入小环DNA载体，将人体细胞重新编程成诱导多能干细胞（iPS细胞）。感兴趣的细胞包括脂肪干细胞。

4. 发明申请

US20110244566A1 Enhanced efficiency of induced pluripotent stem cell generation 有权
标题翻译：诱导多能干细胞生成的效率提高
公开(公告)号：US20110244566A1
公开(公告)日：2011-10-06
申请号：US12931476
申请日：2011-02-01
申请人： Joseph Wu , Michael T. Longaker , Mark A. Kay , Ning Sung , FangJun Jia , Zhi-Ying Chen , Nicholas Panetta , Deepak Gupta
发明人： Joseph Wu , Michael T. Longaker , Mark A. Kay , Ning Sung , FangJun Jia , Zhi-Ying Chen , Nicholas Panetta , Deepak Gupta
IPC分类号： C12N5/074 , C12N15/63
CPC分类号： C12N5/0696 , C12N15/85 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/605 , C12N2501/606 , C12N2506/1384 , C12N2510/00 , C12N2800/00 , C12N2800/108 , C12N2800/24
摘要： Human somatic cells are reprogrammed to become induced pluripotent stem cells (iPS cells) by the introduction of a minicircle DNA vector. Cells of interest include adipose stem cells.
摘要翻译：通过引入小环DNA载体，将人体细胞重新编程成诱导多能干细胞（iPS细胞）。感兴趣的细胞包括脂肪干细胞。

5. 发明授权

US08828726B2 Circular nucleic acid vectors, and methods for making and using the same 有权
标题翻译：圆形核酸载体及其制备和使用方法
公开(公告)号：US08828726B2
公开(公告)日：2014-09-09
申请号：US11398490
申请日：2006-04-04
申请人： Mark A. Kay , Zhi-Ying Chen
发明人： Mark A. Kay , Zhi-Ying Chen
IPC分类号： A61K48/00 , C12N15/85
CPC分类号： C12N15/64 , A61K48/005 , C12N15/70 , C12N15/85 , C12N2800/108 , C12N2800/30 , C12N2800/80 , C12N2830/002 , C12N2830/55
摘要： Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same.
摘要翻译：提供了提供持续高水平蛋白质表达的圆形核酸载体。本发明的圆形载体的特征在于没有表达沉默细菌序列，其中在许多实施方案中，除了表达盒之外，主题载体还包括单向位点特异性重组产物杂合序列。还提供了使用本发明载体将核酸例如表达盒引入靶细胞的方法，以及用于实施这些方法的制剂。主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。还提供了用于生产本发明方法中使用的载体的高效且易于扩展的方法，以及用于实施该载体的试剂和试剂盒/系统。

6. 发明授权

US08236548B2 Minicircle DNA vector preparations and methods of making and using the same 有权
标题翻译：小环DNA载体制剂及其制备和使用方法
公开(公告)号：US08236548B2
公开(公告)日：2012-08-07
申请号：US12497396
申请日：2009-07-02
申请人： Zhi-Ying Chen , Mark A. Kay
发明人： Zhi-Ying Chen , Mark A. Kay
IPC分类号： C12N1/21
CPC分类号： C12N15/70 , A61K48/005 , A61K48/0066 , C07K14/195 , C12N15/85 , C12N2800/108 , C12N2800/30 , C12N2800/80 , C12N2830/002 , C12N2830/55
摘要： The present invention provides minicircle nucleic acid vector formulations for use in administering to a subject, wherein the minicircle nucleic acid vectors include a polynucleotide of interest, a product hybrid sequence of a unidirectional site-specific recombinase, and are devoid of plasmid backbone bacterial DNA sequences. Also provided are methods of producing the subject formulations as well as methods for administering the minicircle nucleic acid vector formulations to a subject. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications.
摘要翻译：本发明提供了用于向受试者施用的小环核酸载体制剂，其中小环核酸载体包括目的多核苷酸，单向位点特异性重组酶的产物杂交序列，并且没有质粒主链细菌DNA序列。还提供了制备本发明制剂的方法以及向受试者施用小环核酸载体制剂的方法。主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。

7. 发明授权

US08124407B2 Circular nucleic acid vectors, and methods for making and using the same 有权
标题翻译：圆形核酸载体及其制备和使用方法
公开(公告)号：US08124407B2
公开(公告)日：2012-02-28
申请号：US13006356
申请日：2011-01-13
申请人： Mark A. Kay , Zhi-Ying Chen
发明人： Mark A. Kay , Zhi-Ying Chen
IPC分类号： C12N15/00
CPC分类号： C12N15/64 , A61K48/005 , C12N15/70 , C12N15/85 , C12N2800/108 , C12N2800/30 , C12N2800/80 , C12N2830/002 , C12N2830/55
摘要： Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same.
摘要翻译：提供了提供持续高水平蛋白质表达的圆形核酸载体。本发明的圆形载体的特征在于没有表达沉默细菌序列，其中在许多实施方案中，除了表达盒之外，主题载体还包括单向位点特异性重组产物杂合序列。还提供了使用本发明载体将核酸例如表达盒引入靶细胞的方法，以及用于实施这些方法的制剂。主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。还提供了用于生产本发明方法中使用的载体的高效且易于扩展的方法，以及用于实施本发明方法的试剂和试剂盒/系统。

8. 发明授权

US08945885B2 Minicircle DNA vector preparations and methods of making and using the same 有权
标题翻译：小环DNA载体制剂及其制备和使用方法
公开(公告)号：US08945885B2
公开(公告)日：2015-02-03
申请号：US13567908
申请日：2012-08-06
申请人： Zhi-Ying Chen , Mark A. Kay
发明人： Zhi-Ying Chen , Mark A. Kay
IPC分类号： C12N15/64 , C12N15/70 , A61K48/00 , C07K14/195 , C12N15/85
CPC分类号： A61K48/0066 , A61K48/005 , C07K14/195 , C12N15/70 , C12N15/85 , C12N2800/108 , C12N2800/24 , C12N2800/30 , C12N2800/80 , C12N2830/002 , C12N2830/55
摘要： The present invention provides minicircle nucleic acid vector formulations for use in administering to a subject, wherein the minicircle nucleic acid vectors include a polynucleotide of interest, a product hybrid sequence of a unidirectional site-specific recombinase, and are devoid of plasmid backbone bacterial DNA sequences. Also provided are methods of producing the subject formulations as well as methods for administering the minicircle nucleic acid vector formulations to a subject. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications.
摘要翻译：本发明提供了用于向受试者施用的小环核酸载体制剂，其中小环核酸载体包括目的多核苷酸，单向位点特异性重组酶的产物杂交序列，并且没有质粒主链细菌DNA序列。还提供了制备本发明制剂的方法以及向受试者施用小环核酸载体制剂的方法。主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。

9. 发明授权

US07897380B2 Circular nucleic acid vectors, and methods for making and using the same 有权
标题翻译：圆形核酸载体及其制备和使用方法
公开(公告)号：US07897380B2
公开(公告)日：2011-03-01
申请号：US10652729
申请日：2003-08-28
申请人： Mark A. Kay , Zhi-Ying Chen
发明人： Mark A. Kay , Zhi-Ying Chen
IPC分类号： C12N15/00
CPC分类号： C12N15/64 , A61K48/005 , C12N15/70 , C12N15/85 , C12N2800/108 , C12N2800/30 , C12N2800/80 , C12N2830/002 , C12N2830/55
摘要： Circular nucleic acid vectors that provide for persistently high levels of protein expression are provided. The circular vectors of the subject invention are characterized by being devoid of expression-silencing bacterial sequences, where in many embodiments the subject vectors include a unidirectional site-specific recombination product hybrid sequence in addition to an expression cassette. Also provided are methods of using the subject vectors for introduction of a nucleic acid, e.g., an expression cassette, into a target cell, as well as preparations for use in practicing such methods. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications. Also provided is a highly efficient and readily scalable method for producing the vectors employed in the subject methods, as well as reagents and kits/systems for practicing the same.
摘要翻译：提供了提供持续高水平蛋白质表达的圆形核酸载体。本发明的圆形载体的特征在于没有表达沉默细菌序列，其中在许多实施方案中，除了表达盒之外，主题载体还包括单向位点特异性重组产物杂合序列。还提供了使用本发明载体将核酸例如表达盒引入靶细胞的方法，以及用于实施这些方法的制剂。主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。还提供了用于生产本发明方法中使用的载体的高效且易于扩展的方法，以及用于实施本发明方法的试剂和试剂盒/系统。

10. 发明申请

US20100075401A1 MINICIRCLE DNA VECTOR PREPARATIONS AND METHODS OF MAKING AND USING THE SAME 有权
标题翻译：小分子DNA载体的制备及其制备和使用方法
公开(公告)号：US20100075401A1
公开(公告)日：2010-03-25
申请号：US12497396
申请日：2009-07-02
申请人： Zhi-Ying Chen , Mark A. Kay
发明人： Zhi-Ying Chen , Mark A. Kay
IPC分类号： C12N1/21 , C12N15/64
CPC分类号： C12N15/70 , A61K48/005 , A61K48/0066 , C07K14/195 , C12N15/85 , C12N2800/108 , C12N2800/30 , C12N2800/80 , C12N2830/002 , C12N2830/55
摘要： The present invention provides minicircle nucleic acid vector formulations for use in administering to a subject, wherein the minicircle nucleic acid vectors include a polynucleotide of interest, a product hybrid sequence of a unidirectional site-specific recombinase, and are devoid of plasmid backbone bacterial DNA sequences. Also provided are methods of producing the subject formulations as well as methods for administering the minicircle nucleic acid vector formulations to a subject. The subject methods and compositions find use in a variety of different applications, including both research and therapeutic applications.
摘要翻译：本发明提供了用于向受试者施用的小环核酸载体制剂，其中小环核酸载体包括目的多核苷酸，单向位点特异性重组酶的产物杂交序列，并且没有质粒主链细菌DNA序列。还提供了制备本发明制剂的方法以及向受试者施用小环核酸载体制剂的方法。主题方法和组合物可用于各种不同的应用，包括研究和治疗应用。

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