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1. 发明申请

US20050026290A1 Inhibiting gene expression with dsRNA 审中-公开
标题翻译：用dsRNA抑制基因表达
公开(公告)号：US20050026290A1
公开(公告)日：2005-02-03
申请号：US10631718
申请日：2003-08-01
申请人： Joseph Ciardi , Kenichi Shibuya , Holly Loucas , David Clark
发明人： Joseph Ciardi , Kenichi Shibuya , Holly Loucas , David Clark
IPC分类号： C12N15/82 , A01H1/00
CPC分类号： C12N15/8249 , C12N15/8218
摘要： The present invention relates to the altering or controlling inhibition of gene expression in a cell using a methodology involving a dsRNA. The use of the method in plants and plant cells has been found to be particularly beneficial. For example the inhibition of a hormone signaling gene in a plant, such as the EIN2 gene, permits the modulating of flower longevity in the selected plant. This is accomplished by using our RNAi technology in inhibiting the expression of an ethylene-modulated gene or an ethylene signaling gene in plants and plant cells. This method provides for the use of RNAi technology in inhibiting the expression of a hormone signaling gene in a plant, particularly in the floral parts of a plant. Thus, one can modulate flower longevity in a plant.
摘要翻译：本发明涉及使用涉及dsRNA的方法改变或控制细胞中基因表达的抑制。已经发现在植物和植物细胞中使用该方法是特别有益的。例如，抑制植物中的激素信号基因，例如EIN2基因，允许调节所选择的植物中的花的寿命。这是通过使用我们的RNAi技术在植物和植物细胞中抑制乙烯调节基因或乙烯信号基因的表达来实现的。该方法提供了使用RNAi技术来抑制植物中特别是植物的花卉部位中激素信号基因的表达。因此，可以调节植物中的花卉寿命。

2. 发明申请

US20050060772A1 Regulation of ethylene sensitivity during leaf and flower abscission 审中-公开
标题翻译：叶和花脱落期乙烯敏感性的调控
公开(公告)号：US20050060772A1
公开(公告)日：2005-03-17
申请号：US10493728
申请日：2002-10-28
申请人： Joseph Ciardi , Robert Cibulsky
发明人： Joseph Ciardi , Robert Cibulsky
IPC分类号： A01H1/00 , C07K14/415 , C12N15/29 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8249
摘要： Provided is the tissue specific manipulation of the EIN2 and EIN3 ethylene-signaling genes, permitting the controlled regulation of leaf and/or flower abscission, since flower and leaf abscission are positively regulated by the plant hormone ethylene. In particular, controlled expression of the EIN2 and EIN3, permits reduction of early-season flower abscission and accelerated late-season leaf abscission, preferably under the control of an inducible promoter. When the altered expression of ethylene signaling genes is combined, for example in a preferred embodiment in the cotton plant, both reduced flower abscission and induced premature leaf abscission are achieved, thereby producing a more productive and more efficiently harvested crop. Further provided are new insights into the mechanisms involved in the ethylene signaling pathway.
摘要翻译：提供了EIN2和EIN3乙烯信号传导基因的组织特异性操作，允许叶和/或花脱落的受控调节，因为花和叶脱落被植物激素乙烯正调控。特别地，EIN2和EIN3的受控表达允许减少早季花脱落和加速后季叶脱落，优选在诱导型启动子的控制下。当乙烯信号转导基因的改变的表达结合时，例如在棉花植物中的优选实施方案中，实现了减少的花脱落和诱导的早熟叶脱落，从而产生更有效和更有效收获的作物。进一步提供的是对乙烯信号通路中涉及的机制的新见解。

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