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    • 1. 发明申请
    • ANNEALING CONTROL PRIMER AND ITS USES
    • 退火控制器及其使用
    • US20120164692A1
    • 2012-06-28
    • US13402980
    • 2012-02-23
    • Jong-Yoon CHUN
    • Jong-Yoon CHUN
    • C12P19/34C07H1/00C07H21/04
    • C12Q1/6876C12Q1/6853C12Q2525/101
    • The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3′-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5′-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3′-end portion and said 5′-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature.
    • 本发明涉及用于提高核酸扩增退火特异性的退火对照引物及其在核酸扩增涉及技术的各个领域的应用。 本发明的引物包含(a)具有与模板核酸上的位点基本上互补的杂交核苷酸序列的3'端部分,以与其杂交; (b)具有预先选择的任意核苷酸序列的5'-端部分; 和(c)位于所述3'-末端部分和所述5'-末端部分之间的调节部分,其包含至少一个通用碱基或非歧视性碱基类似物,由此所述调节部分能够调节所述引物的退火部分 与退火温度有关。