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1. 发明申请

US20090011511A1 Single-Point Genome Signature Tags 审中-公开
标题翻译：单点基因组签名标签
公开(公告)号：US20090011511A1
公开(公告)日：2009-01-08
申请号：US11925382
申请日：2007-10-26
申请人： John J. Dunn , Daniel van der Lelie , Maureen K. Krause , Sean R. McCorkle
发明人： John J. Dunn , Daniel van der Lelie , Maureen K. Krause , Sean R. McCorkle
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6827 , C12Q2525/191 , C12Q2525/131 , C12Q2521/313 , C12Q2537/159 , C12Q2537/164 , C12Q2563/131
摘要： Disclosed is a method for analyzing the organismic complexity of a sample through analysis of the nucleic acid in the sample. In the disclosed method, through a series of steps, including digestion with a type II restriction enzyme, ligation of capture adapters and linkers and digestion with a type IIS restriction enzyme, genome signature tags are produced. The sequences of a statistically significant number of the signature tags are determined and the sequences are used to identify and quantify the organisms in the sample. Various embodiments of the invention described herein include methods for using single point genome signature tags to analyze the related families present in a sample, methods for analyzing sequences associated with hyper- and hypo-methylated CpG islands, methods for visualizing organismic complexity change in a sampling location over time and methods for generating the genome signature tag profile of a sample of fragmented DNA.
摘要翻译：公开了通过分析样品中的核酸来分析样品的生物复杂性的方法。在所公开的方法中，通过一系列步骤，包括用II型限制酶消化，连接捕获适配器和接头并用IIS限制酶消化，产生基因组签名标签。确定统计学上显着数量的签名标签的序列，并且使用该序列来鉴定和量化样品中的生物体。本文描述的本发明的各种实施方案包括使用单点基因组签名标签分析样品中存在的相关家族的方法，用于分析与超低甲基化和次甲基化CpG岛相关的序列的方法，用于可视化取样中的有机体复杂性变化的方法随着时间推移的位置以及用于产生片段化DNA样品的基因组特征标签谱的方法。

2. 发明授权

US08623376B2 Chimeric OspA genes, proteins, and methods of use thereof 有权
标题翻译：嵌合OspA基因，蛋白质及其使用方法
公开(公告)号：US08623376B2
公开(公告)日：2014-01-07
申请号：US13107796
申请日：2011-05-13
申请人： Brian A. Crowe , Ian Livey , Maria O'Rourke , Michael Schwendinger , John J. Dunn , Benjamin J. Luft
发明人： Brian A. Crowe , Ian Livey , Maria O'Rourke , Michael Schwendinger , John J. Dunn , Benjamin J. Luft
IPC分类号： A61K39/40 , C12N15/63 , C12P21/02 , C12N1/19 , C07K14/20 , A61K39/116
CPC分类号： A61K39/0225 , A61K2039/575 , A61K2039/6018 , A61K2039/70 , C07K14/20 , Y02A50/39 , Y02A50/396 , Y02A50/40 , Y02A50/401 , Y02A50/403
摘要： The invention relates to the development of chimeric OpsA molecules for use in a new Lyme vaccine. More specifically, the chimeric OspA molecules comprise the proximal portion from one OspA serotype, together with distal portion from another OspA serotype, while retaining antigenic properties of both of the parent polypeptides. The chimeric OspA molecules are delivered alone or in combination to provide protection against a variety of Borrelia genospecies. The invention also provides methods for administering the chimeric OspA molecules to a subject in the prevention and treatment of Lyme disease or borreliosis.
摘要翻译：本发明涉及用于新的莱姆疫苗的嵌合OpsA分子的开发。更具体地，嵌合OspA分子包含来自一个OspA血清型的近端部分以及来自另一种OspA血清型的远端部分，同时保留两种亲本多肽的抗原性质。嵌合的OspA分子单独或组合递送以提供针对各种疏螺旋体基因属的保护。本发明还提供了在预防和治疗莱姆病或硼状血症中向受试者施用嵌合OspA分子的方法。

3. 发明授权

US08263742B2 Methods for detection of methyl-CpG dinucleotides 有权
标题翻译：检测甲基CpG二核苷酸的方法
公开(公告)号：US08263742B2
公开(公告)日：2012-09-11
申请号：US13085219
申请日：2011-04-12
申请人： John J. Dunn
发明人： John J. Dunn
IPC分类号： C07K14/265
CPC分类号： C12P19/34 , C07K14/245 , C07K2319/40 , C12P19/36 , C12Q1/6827 , C12Q2523/125 , C12Q2522/101
摘要： The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5′-CMeCpGG-3′. Methods for making and using the rMcrA protein, and derivatives thereof are provided.
摘要翻译：本发明提供了从DNA样品中富集甲基CpG序列的方法。该方法利用在保留甲基 - 胞嘧啶残基的条件下将胞嘧啶残基转化成尿嘧啶。本发明的另外的方法能够保存me-CpG二核苷酸的上下文。本发明还提供了用于结合和分离含有5'-CMeCpGG-3'序列的DNA片段的重组全长和基本上纯的McrA蛋白（rMcrA）。提供了制备和使用rMcrA蛋白的方法及其衍生物。

4. 发明申请

US20100081174A1 Methods for Detection of Methyl-CpG Dinucleotides 有权
标题翻译：甲基CpG二核苷酸检测方法
公开(公告)号：US20100081174A1
公开(公告)日：2010-04-01
申请号：US12507522
申请日：2009-07-22
申请人： John J. Dunn
发明人： John J. Dunn
IPC分类号： C12P19/34 , C12P1/04
CPC分类号： C12P19/34 , C07K14/245 , C07K2319/40 , C12P19/36 , C12Q1/6827 , C12Q2523/125 , C12Q2522/101
摘要： The invention provides methods for enriching methyl-CpG sequences from a DNA sample. The method makes use of conversion of cytosine residues to uracil under conditions in which methyl-cytosine residues are preserved. Additional methods of the invention enable to preservation of the context of me-CpG dinucleotides. The invention also provides a recombinant, full length and substantially pure McrA protein (rMcrA) for binding and isolation of DNA fragments containing the sequence 5′-CMeCpGG-3′. Methods for making and using the rMcrA protein, and derivatives thereof are provided.
摘要翻译：本发明提供了从DNA样品中富集甲基CpG序列的方法。该方法利用在保留甲基 - 胞嘧啶残基的条件下将胞嘧啶残基转化成尿嘧啶。本发明的另外的方法能够保存me-CpG二核苷酸的上下文。本发明还提供了用于结合和分离含有5'-CMeCpGG-3'序列的DNA片段的重组全长和基本上纯的McrA蛋白（rMcrA）。提供了制备和使用rMcrA蛋白的方法及其衍生物。

5. 发明申请

US20090142218A1 LEAN AUSTENITIC STAINLESS STEEL 有权
标题翻译：精益奥氏不锈钢
公开(公告)号：US20090142218A1
公开(公告)日：2009-06-04
申请号：US12037477
申请日：2008-02-26
申请人： David S. Bergstrom , James M. Rakowski , Charles P. Stinner , John J. Dunn , John F. Grubb
发明人： David S. Bergstrom , James M. Rakowski , Charles P. Stinner , John J. Dunn , John F. Grubb
IPC分类号： C22C38/58 , C22C38/44
CPC分类号： C22C38/58 , C22C38/001 , C22C38/002 , C22C38/02 , C22C38/22 , C22C38/30 , C22C38/32 , C22C38/34 , C22C38/38 , C22C38/42 , C22C38/44 , C22C38/52 , C22C38/54
摘要： An austenitic stainless steel having low nickel and molybdenum and exhibiting comparable corrosion resistance and formability properties to higher nickel and molybdenum alloys comprises, in weight %, up to 0.20 C, 2.0-9.0 Mn, up to 2.0 Si, 16.0-23.0 Cr, 1.0-5.0 Ni, up to 3.0 Mo, up to 3.0 Cu, 0.1-0.35 N, up to 4.0 W, up to 0.01 B, up to 1.0 Co, iron and impurities, the steel having a ferrite number of less than 10 and a MD30 value of less than 20° C.
摘要翻译：具有低镍和钼的奥氏体不锈钢并且具有与较高镍和钼合金相当的耐腐蚀性和成形性能的奥氏体不锈钢包括以重量％计高达0.20C，2.0-9.0Mn，高达2.0Si，16.0-23.0Cr，1.0 -5.0Ni，高达3.0Mo，至多3.0Cu，0.1-0.35N，至多4.0W，至多0.01B，至多1.0Co，铁和杂质，所述钢的铁素体数小于10， MD30值小于20°C

6. 发明授权

US07179448B2 Recombinant constructs of Borrelia burgdorferi 失效
标题翻译：博氏疏螺旋体的重组构建体
公开(公告)号：US07179448B2
公开(公告)日：2007-02-20
申请号：US11196475
申请日：2005-08-03
申请人： Raymond J. Dattwyler , Maria J. C. Gomes-Solecki , Benjamin J. Luft , John J. Dunn
发明人： Raymond J. Dattwyler , Maria J. C. Gomes-Solecki , Benjamin J. Luft , John J. Dunn
IPC分类号： A61K39/00 , A61K39/02 , A61K39/38 , A61K49/00
CPC分类号： C07K16/1207 , A61K39/00 , A61K2039/53 , C07K14/20 , C07K2319/00 , Y02A50/401
摘要： Novel chimeric nucleic acids, encoding chimeric Borrelia proteins comprising OspC or an antigenic fragment thereof and OspA or an antigenic fragment thereof, are disclosed. Chimeric proteins encoded by the nucleic acid sequences are also disclosed. The chimeric proteins are useful as vaccine immunogens against Lyme borreliosis, as well as for immunodiagnostic reagents.
摘要翻译：公开了编码包含OspC或其抗原性片段的嵌合型疏螺旋体蛋白和OspA或其抗原性片段的新型嵌合核酸。还公开了由核酸序列编码的嵌合蛋白。嵌合蛋白质可用作针对莱姆硼芽孢杆菌病的疫苗免疫原，以及用于免疫诊断试剂。

7. 发明授权

US06623569B2 Duplex stainless steels 有权
公开(公告)号：US06623569B2
公开(公告)日：2003-09-23
申请号：US10238182
申请日：2002-09-10
申请人： David S. Bergstrom , John J. Dunn , John F. Grubb , William A. Pratt
发明人： David S. Bergstrom , John J. Dunn , John F. Grubb , William A. Pratt
IPC分类号： C22C3858
CPC分类号： C21D6/004 , C21D2211/001 , C21D2211/005 , C22C38/001 , C22C38/44 , C22C38/54 , C22C38/58
摘要： A duplex stainless steel includes less than, in weight percent, 3 percent nickel and 1.5 percent molybdenum. In one embodiment, the duplex stainless steel includes, in weight percent, up to 0.06 percent carbon; 15 to 25 percent chromium; 1 to less than 2.5 percent nickel; greater than 2 percent up to 3.75 percent manganese; greater than 0.12 up to 0.35 percent nitrogen; up to 2 percent silicon; up to 1.5 percent molybdenum; up to 0.5 percent copper; up to 0.2 percent cobalt; up to 0.05 percent phosphorous; up to 0.005 percent sulfur; 0.001 to 0.0035 percent boron; iron and incidental impurities. The duplex stainless steel provided may be provided in the form of an article of manufacture, such as strip, bar, plate, sheet, casting, tubing or piping. A method for making the duplex stainless steel of the invention also is disclosed.

8. 发明授权

US08337748B2 Lean austenitic stainless steel containing stabilizing elements 有权
标题翻译：精益奥氏体不锈钢含稳定元素
公开(公告)号：US08337748B2
公开(公告)日：2012-12-25
申请号：US12034183
申请日：2008-02-20
申请人： James M. Rakowski , David S. Bergstrom , Charles P. Stinner , John J. Dunn , John F. Grubb
发明人： James M. Rakowski , David S. Bergstrom , Charles P. Stinner , John J. Dunn , John F. Grubb
IPC分类号： C22C38/48 , C22C38/50 , C22C38/58
CPC分类号： C22C38/58 , C22C38/001 , C22C38/002 , C22C38/02 , C22C38/38 , C22C38/42 , C22C38/44 , C22C38/48 , C22C38/50 , C22C38/52 , C22C38/54 , F16L11/15 , F23J13/02
摘要： An austenitic stainless steel composition including relatively low nickel and molybdenum levels, and exhibiting corrosion resistance, resistance to elevated temperature deformation, and formability properties comparable to certain alloys including higher nickel and molybdenum levels. Embodiments of the austenitic stainless steel include, in weight %, up to 0.20 C, 2.0-9.0 Mn, up to 2.0 Si, 16.0-23.0 Cr, 1.0-7.0 Ni, up to 3.0 Mo, up to 3.0 Cu, 0.05-0.35 N, up to 4.0 W, (7.5(% C))≦(Nb+Ti+V+Ta+Zr)≦1.5, up to 0.01 B, up to 1.0 Co, iron and impurities. Additionally, embodiments of the steel may include 0.5≦(Mo+W/2)≦5.0 and/or 1.0≦(Ni+Co)≦8.0.
摘要翻译：奥氏体不锈钢组合物包括较低的镍和钼含量，具有耐腐蚀性，耐高温变形性，以及与包括较高镍和钼含量的某些合金相当的成型性能。奥氏体不锈钢的实施例包括重量％，最高0.20C，2.0-9.0Mn，至多2.0Si，16.0-23.0Cr，1.0-7.0Ni，至多3.0Mo，至多3.0Cu，0.05-0.35 N，高达4.0W，（7.5（％C））＆nlE;（Nb + Ti + V + Ta + Zr）＆nlE; 1.5，至多0.01B，至多1.0 Co，铁和杂质。另外，钢的实施例可以包括0.5＆lt; NlE（Mo + W / 2）＆nlE; 5.0和/或1.0＆nlE;（Ni + Co）＆nlE; 8.0。

9. 发明申请

US20090326200A1 Altered ospa of borrelia burgdorferi 有权
标题翻译：改变勃氏疏螺旋体的ospa
公开(公告)号：US20090326200A1
公开(公告)日：2009-12-31
申请号：US12313443
申请日：2008-11-19
申请人： Benjamin J. Luft , John J. Dunn , Shohei Koide , Catherine L. Lawson
发明人： Benjamin J. Luft , John J. Dunn , Shohei Koide , Catherine L. Lawson
IPC分类号： C07K14/195
CPC分类号： C07K14/20 , A61K39/00 , C07K2319/00 , Y02A50/401
摘要： Provided herein are OspA polypeptides from Lyme Disease-causing Borrelia having certain alteration(s). In one embodiment, the alteration(s) increase the conformational stability of the OspA polypeptide containing the alteration(s) while maintaining at least some of the antigenicity of the corresponding unaltered OspA polypeptide. In another embodiment, the altered OspA polypeptide has reduced cross-reactivity to hLFA-1, as compared to the corresponding unaltered OspA polypeptide.
摘要翻译：本文提供了具有某些改变的来自莱姆病引起的疏螺旋体的OspA多肽。在一个实施方案中，所述改变增加含有所述改变的OspA多肽的构象稳定性，同时保持相应未改变的OspA多肽的至少一些抗原性。在另一个实施方案中，与相应的未改变的OspA多肽相比，改变的OspA多肽具有降低的与hLFA-1的交叉反应性。

10. 发明申请

US20090162237A1 LEAN AUSTENITIC STAINLESS STEEL CONTAINING STABILIZING ELEMENTS 有权
标题翻译：含有稳定元素的奥氏体不锈钢
公开(公告)号：US20090162237A1
公开(公告)日：2009-06-25
申请号：US12034183
申请日：2008-02-20
申请人： James M. Rakowski , David S. Bergstrom , Charles P. Stinner , John J. Dunn , John F. Grubb
发明人： James M. Rakowski , David S. Bergstrom , Charles P. Stinner , John J. Dunn , John F. Grubb
IPC分类号： C22C38/58 , C22C38/44
CPC分类号： C22C38/58 , C22C38/001 , C22C38/002 , C22C38/02 , C22C38/38 , C22C38/42 , C22C38/44 , C22C38/48 , C22C38/50 , C22C38/52 , C22C38/54 , F16L11/15 , F23J13/02
摘要： An austenitic stainless steel composition including relatively low nickel and molybdenum levels, and exhibiting corrosion resistance, resistance to elevated temperature deformation, and formability properties comparable to certain alloys including higher nickel and molybdenum levels. Embodiments of the austenitic stainless steel include, in weight %, up to 0.20 C, 2.0-9.0 Mn, up to 2.0 Si, 16.0-23.0 Cr, 1.0-7.0 Ni, up to 3.0 Mo, up to 3.0 Cu, 0.05-0.35 N, up to 4.0 W, (7.5(% C))≦(Nb+Ti+V+Ta+Zr)≦1.5, up to 0.01 B, up to 1.0 Co, iron and impurities. Additionally, embodiments of the steel may include 0.5≦(Mo+W/2)≦5.0 and/or 1.0≦(Ni+Co)≦8.0.
摘要翻译：奥氏体不锈钢组合物包括较低的镍和钼含量，具有耐腐蚀性，耐高温变形性，以及与包括较高镍和钼含量的某些合金相当的成型性能。奥氏体不锈钢的实施例包括重量％，最高0.20C，2.0-9.0Mn，至多2.0Si，16.0-23.0Cr，1.0-7.0Ni，至多3.0Mo，至多3.0Cu，0.05-0.35 N，高达4.0W，（7.5（％C））<=（Nb + Ti + V + Ta + Zr）<= 1.5，至多0.01B，至多1.0 Co，铁和杂质。另外，钢的实施例可以包括0.5 <=（Mo + W / 2）≤5.0和/或1.0 <=（Ni + Co）≤8.0。

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