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1. 发明授权

US10017774B2 Control of pests in plants 有权
公开(公告)号：US10017774B2
公开(公告)日：2018-07-10
申请号：US13497729
申请日：2010-09-15
申请人： Jian Ye , Nam-Hai Chua , Jing Qu , Shi-Qiang Gao
发明人： Jian Ye , Nam-Hai Chua , Jing Qu , Shi-Qiang Gao
IPC分类号： C12N15/82
CPC分类号： C12N15/8218 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： The present invention relates to the field of controlling pests, such as insects, using a virus to express pest genes in hosts. More specifically, the present invention relates to a method for rapidly screening for pest genes which can lead to mortality of the pest when the pest has ingested host tissues expressing virus-linked pest gene sequences. The present invention also relates to a method for controlling pests by viral expression of target pest sequences to modify endogenous expression of pest genes in cells or tissues of the pest.

2. 发明申请

US20120240288A1 CONTROL OF PESTS IN PLANTS 有权
标题翻译：植物中的植物控制
公开(公告)号：US20120240288A1
公开(公告)日：2012-09-20
申请号：US13497729
申请日：2010-09-15
申请人： Jian Ye , Nam-Hai Chua , Jing Qu , Shi-Qiang Gao
发明人： Jian Ye , Nam-Hai Chua , Jing Qu , Shi-Qiang Gao
IPC分类号： A01H1/06 , A61K49/00
CPC分类号： C12N15/8218 , C12N15/8285 , C12N15/8286 , Y02A40/162 , Y02A40/164
摘要： The present invention relates to the field of controlling pests, such as insects, using a virus to express pest genes in hosts. More specifically, the present invention relates to a method for rapidly screening for pest genes which can lead to mortality of the pest when the pest has ingested host tissues expressing virus-linked pest gene sequences. The present invention also relates to a method for controlling pests by viral expression of target pest sequences to modify endogenous expression of pest genes in cells or tissues of the pest.
摘要翻译：本发明涉及使用病毒来在宿主中表达有害生物基因的昆虫等害虫防治领域。更具体地说，本发明涉及一种用于快速筛选有害生物基因的方法，当害虫摄入表达病毒连接的害虫基因序列的宿主组织时，可导致害虫死亡。本发明还涉及通过靶病毒序列的病毒表达来控制害虫的方法，以修饰害虫的细胞或组织中害虫基因的内源性表达。

3. 发明申请

US20110265223A1 FUNCTIONAL ANALYSIS OF JATROPHA CURCAS GENES 有权
标题翻译： JATROPHA CURCAS基因的功能分析
公开(公告)号：US20110265223A1
公开(公告)日：2011-10-27
申请号：US13141752
申请日：2009-12-16
申请人： Jian Ye , Nam Hai Chua , JIng Qu
发明人： Jian Ye , Nam Hai Chua , JIng Qu
IPC分类号： A01H3/00 , C12Q1/68
CPC分类号： C12N15/8218
摘要： The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).
摘要翻译：本发明涉及基因组尺度上的麻疯树基因的功能分析领域。更具体地，本发明涉及使用病毒诱导的基因沉默在基因组规模上高麻醉麻疯树基因的高通量功能分析的方法。该方法涉及使用烟草拨浪鼓病毒（TRV）。

4. 发明授权

US08921655B2 Functional analysis of Jatropha curcas genes 有权
标题翻译：麻疯树基因的功能分析
公开(公告)号：US08921655B2
公开(公告)日：2014-12-30
申请号：US13141752
申请日：2009-12-16
申请人： Jian Ye , Nam Hai Chua , Jing Qu
发明人： Jian Ye , Nam Hai Chua , Jing Qu
IPC分类号： C12N15/11 , C07H21/04 , C12N15/113 , C12N15/63 , C12N15/82 , C12N15/83 , C12N15/84 , A01H5/00
CPC分类号： C12N15/8218
摘要： The present invention relates to the field of functional analysis of Jatropha genes on a genomic scale. More specifically, the present invention relates to a method for high-throughtput functional analysis of Jatropha curcas genes on a genomic scale using virus-induced gene silencing. The method involves use of the tobacco rattle virus (TRV).
摘要翻译：本发明涉及基因组尺度上的麻疯树基因的功能分析领域。更具体地，本发明涉及使用病毒诱导的基因沉默在基因组规模上高麻醉麻疯树基因的高通量功能分析的方法。该方法涉及使用烟草拨浪鼓病毒（TRV）。

5. 发明授权

US08853493B2 Virus induced gene silencing (VIGS) for functional analysis of genes in cotton 有权
标题翻译：病毒诱导的基因沉默（VIGS）用于棉花基因的功能分析
公开(公告)号：US08853493B2
公开(公告)日：2014-10-07
申请号：US13376902
申请日：2010-06-10
申请人： Jian Ye , Nam Hai Chua , Jing Qu , Yun Feng Geng , Yun Ping Bu
发明人： Jian Ye , Nam Hai Chua , Jing Qu , Yun Feng Geng , Yun Ping Bu
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8218 , C12N15/8261 , Y02A40/146
摘要： The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.
摘要翻译：本发明涉及使用病毒诱导基因沉默（VIGS）方法对棉花中基因的功能分析。更具体地说，这种方法在烟草叮咬病毒（TRV）载体RNA1和RNA2的帮助下诱导棉花中的基因沉默，并且可以分析对棉花植物的表型效应。此外，本发明还提供瞬时表达载体TRV RNA2以瞬时表达基因在棉花植物和植物组织中受到强亚基因子启动子的影响。

6. 发明授权

US10100321B2 Methods for increasing cotton fiber length 有权
公开(公告)号：US10100321B2
公开(公告)日：2018-10-16
申请号：US14395233
申请日：2012-04-19
申请人： Nam-Hai Chua , Jian Ye , Jing Qu
发明人： Nam-Hai Chua , Jian Ye , Jing Qu
IPC分类号： C12N15/82 , C07K14/415
摘要： The present invention relates to the field of plant molecular biology, more particularly cotton WRINKLED 1-like (WREL) genes. More specifically, the present invention relates to cotton WRIL genes whose products act as transcription factors of genes involved in fatty acid biosynthesis. The present invention also relates to methods of increasing cotton fiber length in cotton. In one embodiment, the methods involve modulating the level of activity of an enzyme involved in a fatty acid biosynthesis in the host cotton cell and/or culturing the host cotton cell. In another embodiment, the methods involve the manipulation of transcription factors which can regulate an enzyme involved in fatty acid biosynthesis.

7. 发明申请

US20150074853A1 METHODS FOR INCREASING COTTON FIBER LENGTH 审中-公开
标题翻译：增加纤维长度的方法
公开(公告)号：US20150074853A1
公开(公告)日：2015-03-12
申请号：US14395233
申请日：2012-04-19
申请人： Nam-Hai Chua , Jian Ye , Jing Qu
发明人： Nam-Hai Chua , Jian Ye , Jing Qu
IPC分类号： C12N15/82
CPC分类号： C12N15/8218 , C07K14/415 , C12N15/8234 , C12N15/8247 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to the field of plant molecular biology, more particularly cotton WRINKLED 1-like (WREL) genes. More specifically, the present invention relates to cotton WRIL genes whose products act as transcription factors of genes involved in fatty acid biosynthesis. The present invention also relates to methods of increasing cotton fiber length in cotton. In one embodiment, the methods involve modulating the level of activity of an enzyme involved in a fatty acid biosynthesis in the host cotton cell and/or culturing the host cotton cell. In another embodiment, the methods involve the manipulation of transcription factors which can regulate an enzyme involved in fatty acid biosynthesis.
摘要翻译：本发明涉及植物分子生物学领域，尤其涉及棉花WRINKLED1样（WRINKLED 1-like，WREL）基因。更具体地说，本发明涉及棉花WRIL基因，其产品作为参与脂肪酸生物合成的基因的转录因子。本发明还涉及增加棉花纤维长度的方法。在一个实施方案中，所述方法包括调节参与宿主棉花细胞中脂肪酸生物合成的酶的活性水平和/或培养宿主棉花细胞。在另一个实施方案中，所述方法包括操纵能够调节参与脂肪酸生物合成的酶的转录因子。

8. 发明申请

US20120096595A1 VIRUS INDUCED GENE SILENCING (VIGS) FOR FUNCTIONAL ANALYSIS OF GENES IN COTTON 有权
标题翻译：用于基因功能分析的病毒基因沉默（VIGS）
公开(公告)号：US20120096595A1
公开(公告)日：2012-04-19
申请号：US13376902
申请日：2010-06-10
申请人： Jian Ye , Nam Hai Chua , Jing Qu , Yun Feng Geng , Yun Ping Bu
发明人： Jian Ye , Nam Hai Chua , Jing Qu , Yun Feng Geng , Yun Ping Bu
IPC分类号： A01H1/06 , C12Q1/68 , C12N15/82 , G01N33/53
CPC分类号： C12N15/8218 , C12N15/8261 , Y02A40/146
摘要： The invention relates to the functional analysis of genes in cotton by employing the Virus Induced Gene Silencing (VIGS) method. More specifically this method induces gene silencing in cotton with the help of the Tobacco Rattle Virus (TRV) vectors RNA1 and RNA2 and phenotypic effects on the cotton plant can be analysed Moreover this invention also provides transient expression vector TRV RNA2 in order to transiently express genes in cotton plants and plant tissue under the influence of a strong subgenomic promoter.
摘要翻译：本发明涉及使用病毒诱导基因沉默（VIGS）方法对棉花中基因的功能分析。更具体地说，这种方法在烟草叮咬病毒（TRV）载体RNA1和RNA2的帮助下诱导棉花中的基因沉默，并且可以分析对棉花植物的表型效应。此外，本发明还提供瞬时表达载体TRV RNA2以瞬时表达基因在棉花植物和植物组织中受到强亚基因子启动子的影响。

9. 发明授权

US08686223B2 Genetic transformation of jatropha curcas 有权
标题翻译：麻疯树的遗传转化
公开(公告)号：US08686223B2
公开(公告)日：2014-04-01
申请号：US13541142
申请日：2012-07-03
申请人： Hui Zhu Mao , Jian Ye , Nam Hai Chua
发明人： Hui Zhu Mao , Jian Ye , Nam Hai Chua
IPC分类号： C07K14/415 , C12N15/10 , A61K38/00 , C12N15/29 , C12N15/84
CPC分类号： C12N15/8205
摘要： The present invention relates to methods for the regeneration and Agrobacterium-mediated transformation of plants in the genera of Jatropha, more specifically, in Jatropha curcas.
摘要翻译：本发明涉及麻疯树属中再生和农杆菌介导转化植物的方法，更具体地，涉及麻疯树。

10. 发明申请

US20110247099A1 GENETIC TRANSFORMATION OF JATROPHA CURCAS 有权
标题翻译： JATROPHA CURCAS的遗传转化
公开(公告)号：US20110247099A1
公开(公告)日：2011-10-06
申请号：US13139592
申请日：2009-12-15
申请人： Hui Zhu Mao , Jian Ye , Nam Hai Chua
发明人： Hui Zhu Mao , Jian Ye , Nam Hai Chua
IPC分类号： C12N15/82 , C12N5/04 , A01G1/00 , A01H4/00
CPC分类号： C12N15/8205
摘要： The present invention relates to methods for the regeneration and Agrobacterium-mediated transformation of plants in the genera of Jatropha, more specifically, in Jatropha curcas.
摘要翻译：本发明涉及麻疯树属中再生和农杆菌介导转化植物的方法，更具体地，涉及麻疯树。

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