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1. 发明申请

US20060101540A1 Methods of transforming corn 审中-公开
标题翻译：玉米转化方法
公开(公告)号：US20060101540A1
公开(公告)日：2006-05-11
申请号：US11280890
申请日：2005-11-16
申请人： Jerome Ranch , Wallace Marsh , Dwight Tomes , Zuo-Yu Zhao
发明人： Jerome Ranch , Wallace Marsh , Dwight Tomes , Zuo-Yu Zhao
IPC分类号： A01H5/00 , A01H1/00
CPC分类号： C12N15/8202 , A01H1/04 , A01H5/10 , C12N15/8201 , C12N15/8205 , C12N15/8207 , C12N15/8209 , C12Q1/6855 , C12Q1/6895 , C12Q2600/156
摘要： Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.
摘要翻译：提供了用于植物转化，提高转化效率和用于产生转基因植物的方法。所述方法包括将受感染植物从感兴趣的植物物种的遗传系与选自相同植物物种的转基因遗传系或另一密切相关的植物物种的供体植物杂交以获得杂种植物。从混合植物获得的组织是转化能力。然后可以用一个或多个感兴趣的核苷酸序列转化这些组织，并选择用于整合在来自受体植物的染色体内的感兴趣的核苷酸序列的转基因事件。可以选择转化的细胞，并再生转基因杂交植物。感兴趣的核苷酸序列可以渗入原始受体亲本来源的基因系，或其他遗传系。另外提供转化的植物和种子。

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