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    • 2. 发明授权
    • FET-type biosensor with surface modification
    • 具有表面改性的FET型生物传感器
    • US08735077B2
    • 2014-05-27
    • US11336110
    • 2006-01-20
    • Jeo-young ShimSu-hyeon KimKyu-tae YooSung-ouk JungJoon-shik Park
    • Jeo-young ShimSu-hyeon KimKyu-tae YooSung-ouk JungJoon-shik Park
    • G01N33/53
    • B82Y30/00B82Y15/00G01N27/4145
    • Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect a signal within the debye length because a target nucleic acid is directly fixed to the surface of a gate of the conventional FET. However, in the present invention, this problem can be overcome and the debye length can be increased by treating the surface of a gate of an FET sensor with a ligand that can bind to a side of a nucleic acid. The ligand can be adsorbed onto the surface of the gate. In this case, the nucleic acid is adsorbed parallel to the surface of the gate, not perpendicular to the surface of the gate, thus generating an effective depletion region. In addition, hybridization efficiency can be increased because a hybridized sample can be injected into an FET sensor at high ionic strength.
    • 提供了包括源电极,栅极和漏电极的场效应晶体管(FET)型生物传感器。 可以结合核酸一侧的配体加入到门的表面。 在传统的FET型生物传感器中,由于目标核酸直接固定在常规FET的栅极表面,难以检测德拜长度内的信号。 然而,在本发明中,可以克服这个问题,并且可以通过用可以结合核酸一侧的配体处理FET传感器的栅极的表面来增加德拜长度。 配体可以吸附在栅极的表面上。 在这种情况下,核酸被平行于栅极的表面吸附,而不垂直于栅极的表面,从而产生有效的耗尽区域。 此外,杂交效率可以提高,因为杂化样品可以以高离子强度注入FET传感器。
    • 3. 发明申请
    • FET-type biosensor with surface modification
    • 具有表面改性的FET型生物传感器
    • US20060205013A1
    • 2006-09-14
    • US11336110
    • 2006-01-20
    • Jeo-young ShimSu-hyeon KimKyu-tae YooSung-ouk JungJoon-shik Park
    • Jeo-young ShimSu-hyeon KimKyu-tae YooSung-ouk JungJoon-shik Park
    • G01N33/53
    • B82Y30/00B82Y15/00G01N27/4145
    • Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect a signal within the debye length because a target nucleic acid is directly fixed to the surface of a gate of the conventional FET. However, in the present invention, this problem can be overcome and the debye length can be increased by treating the surface of a gate of an FET sensor with a ligand that can bind to a side of a nucleic acid. The ligand can be adsorbed onto the surface of the gate. In this case, the nucleic acid is adsorbed parallel to the surface of the gate, not perpendicular to the surface of the gate, thus generating an effective depletion region. In addition, hybridization efficiency can be increased because a hybridized sample can be injected into an FET sensor at high ionic strength.
    • 提供了包括源电极,栅极和漏电极的场效应晶体管(FET)型生物传感器。 可以结合核酸一侧的配体加入到门的表面。 在传统的FET型生物传感器中,由于目标核酸直接固定在常规FET的栅极表面,难以检测德拜长度内的信号。 然而,在本发明中,可以克服这个问题,并且可以通过用可以结合核酸一侧的配体处理FET传感器的栅极的表面来增加德拜长度。 配体可以吸附在栅极的表面上。 在这种情况下,核酸被平行于栅极的表面吸附,而不垂直于栅极的表面,从而产生有效的耗尽区域。 此外,杂交效率可以提高,因为杂化样品可以以高离子强度注入FET传感器。
    • 8. 发明申请
    • Method of increasing discrimination for single base mismatch using hurdle DNA and artificially mismatched probe
    • 使用障碍DNA和人为错配探针增加单碱基错配的鉴别方法
    • US20060183142A1
    • 2006-08-17
    • US11335229
    • 2006-01-19
    • Soo-hyung ChoiJang-seok MaJoon-shik Park
    • Soo-hyung ChoiJang-seok MaJoon-shik Park
    • C12Q1/68C12P19/34
    • C12Q1/6827C12Q2537/113C12Q2527/107C12Q2525/185
    • A method of increasing discrimination for a target DNA having a polymorphic site is provided. The method includes: synthesizing a first probe by artificially substituting a base of a probe which is fully complementary to a target DNA having a polymorphic site by a mismatched natural base and synthesizing a second probe by artificially substituting a base of another probe having a single base mismatch at a position corresponding to the polymorphic site of the target DNA with a mismatched natural base; synthesizing a first hurdle DNA which is fully complementary to the first probe and is shorter than the first probe and a second hurdle DNA which is fully complementary to the second probe and is shorter than the second probe; immobilizing the first and second probes on a substrate; hybridizing the immobilized first and second probes with the first and second hurdle DNAs, respectively; and hybridizing the target DNA with the hybrids. The addition of a hurdle DNA and variation of a probe base can improve an ability of discriminating a single base mismatch.
    • 提供了增加具有多态位点的靶DNA的鉴别方法。 该方法包括:通过人造取代与具有多态性位点的目标DNA完全互补的基因的错配天然碱合成第一探针,并通过人造地取代具有单个碱基的另一种探针的碱合成第二探针 在对应于具有错配天然碱基的目标DNA的多态性位点的位置处的错配; 合成与第一探针完全互补并且比第一探针短的第一障碍DNA和与第二探针完全互补并且短于第二探针的第二障碍DNA; 将第一和第二探针固定在基底上; 将固定的第一和第二探针分别与第一和第二障碍DNA杂交; 并将靶DNA与杂交体杂交。 障碍DNA的添加和探针碱基的变异可以提高鉴别单一碱基不匹配的能力。