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1. 发明申请

US20050150619A1 Xylanase treatment of chemical pulp 有权
标题翻译：木聚糖酶处理化学纸浆
公开(公告)号：US20050150619A1
公开(公告)日：2005-07-14
申请号：US10505815
申请日：2003-03-05
申请人： Jeffrey Tolan , Corina Popovici
发明人： Jeffrey Tolan , Corina Popovici
IPC分类号： D21C5/00 , D21C9/10 , D21C9/12 , D21C9/14
CPC分类号： D21C5/005 , D21C9/10 , D21C9/1036 , D21C9/14
摘要： The present invention discloses methods of bleaching chemical pulp that use xylanase enzymes after chemical bleaching. The method comprises the steps of carrying out a chlorine dioxide stage to produce a partially bleached pulp, treating the partially bleached pulp with a xylanase enzyme, optionally in the presence of oxygen and hydrogen peroxide, in a mild extraction stage, then bleaching the pulp with a second chlorine dioxide stage. The method allows the mill to decrease the usage of sodium hydroxide or other alkali, while decreasing the use of chlorine dioxide, and possibly improving the yield and strength of the pulp, while maintaining a similar level of bleached brightness of the pulp. The pulp bleaching method of the present invention may be performed in a pulp mill as part of a complex pulp bleaching process.
摘要翻译：本发明公开了化学漂白后使用木聚糖酶的漂白化学纸浆的方法。该方法包括以下步骤：进行二氧化氯阶段以产生部分漂白的纸浆，在温和萃取阶段任选在氧气和过氧化氢存在下，用木聚糖酶处理部分漂白的纸浆，然后用纸浆漂白纸浆第二个二氧化氯阶段。该方法允许研磨机降低氢氧化钠或其他碱的用量，同时减少二氧化氯的使用，并且可能提高纸浆的产率和强度，同时保持纸浆的漂白亮度相似水平。本发明的纸浆漂白方法可以在纸浆厂中进行，作为复杂纸浆漂白方法的一部分。

2. 发明申请

US20060154352A1 Upflow settling reactor for enzymatic hydrolysis of cellulose 失效
标题翻译：用于纤维素酶水解的上流沉降反应器
公开(公告)号：US20060154352A1
公开(公告)日：2006-07-13
申请号：US11303424
申请日：2005-12-16
申请人： Brian Foody , Ziyad Rahme , Jeffrey Tolan
发明人： Brian Foody , Ziyad Rahme , Jeffrey Tolan
IPC分类号： C12P7/06 , C12P19/02
CPC分类号： C12P19/14 , C12M45/09 , C12P19/02 , C12P19/04 , Y02E50/16 , Y02E50/17
摘要： A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product from a pre-treated cellulosic feedstock is provided. The process comprises introducing an aqueous slurry of the pre-treated cellulosic feedstock at the bottom of a hydrolysis reactor. Axial dispersion in the reactor is limited by avoiding mixing and maintaining an average slurry flow velocity of about 0.1 to about 20 feet per hour, such that the undissolved solids flow upward at a rate slower than that of the liquid. Cellulase enzymes are added to the aqueous slurry before or during the step of introducing. An aqueous stream comprising hydrolysis product and unhydrolyzed solids is removed from the hydrolysis reactor. Also provided are enzyme compositions which comprise cellulase enzymes and flocculents for use in the process. In addition, a kit comprising cellulase enzymes and flocculent is provided.
摘要翻译：提供了一种纤维素酶水解从预处理的纤维素原料产生水解产物的方法。该方法包括在水解反应器的底部引入预处理的纤维素原料的含水浆料。通过避免混合并保持约0.1至约20英尺/小时的平均浆料流速，使得未溶解的固体以比液体慢的速率向上流动，来限制反应器中的轴向分散。在引入步骤之前或期间，将纤维素酶加入到含水浆料中。从水解反应器中除去包含水解产物和未水解固体的水流。还提供了包含用于该方法的纤维素酶和絮凝剂的酶组合物。此外，提供了包含纤维素酶和絮状物的试剂盒。

3. 发明授权

US08202709B2 Enzyme compositions and methods for the improved enzymatic hydrolysis of cellulose 失效
标题翻译：用于改进纤维素酶水解的酶组合物和方法
公开(公告)号：US08202709B2
公开(公告)日：2012-06-19
申请号：US12305069
申请日：2007-06-22
申请人： Jeffrey Tolan , Theresa White , John Tomashek
发明人： Jeffrey Tolan , Theresa White , John Tomashek
IPC分类号： C12P19/14 , C12N9/42 , C12N9/24 , C02F3/34 , C08B37/00
CPC分类号： C12N9/2437 , C12N9/2445 , C12P7/10 , C12P19/02 , C12Y302/01021 , Y02E50/16 , Y02P20/582
摘要： A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises partially hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one β-glucosidase enzyme and a binding agent for binding the β-glucosidase enzyme to fiber solids present in the aqueous slurry. The unhydrolyzed fiber solids are then separated from the hydrolyzed slurry. The separated fiber solids thus obtained are then re-suspended in an aqueous solution to produce a re-suspended slurry. The hydrolysis is then continued to produce the hydrolysis product comprising glucose.
摘要翻译：提供了纤维素酶水解以产生包含来自预处理的木质纤维素原料的葡萄糖和用于该方法的酶的水解产物的方法。该方法包括用纤维素酶，一种或多于一种葡糖苷酶和用于将葡糖苷酶与含水浆料中存在的纤维固体结合的结合剂部分水解预处理的木质纤维素原料的含水浆料。然后将未水解的纤维固体与水解浆料分离。然后将由此获得的分离的纤维固体重新悬浮在水溶液中以产生再悬浮的浆料。然后继续水解以产生包含葡萄糖的水解产物。

4. 发明申请

US20050244934A1 Recovery of inorganic salt during processing of lignocellulosic feedstocks 有权
标题翻译：在木质纤维素原料加工过程中回收无机盐
公开(公告)号：US20050244934A1
公开(公告)日：2005-11-03
申请号：US11104698
申请日：2005-04-13
申请人： Brian Foody , Jeffrey Tolan , Ziyad Rahme , Vijay Anand
发明人： Brian Foody , Jeffrey Tolan , Ziyad Rahme , Vijay Anand
IPC分类号： B01B1/00 , B01D1/00 , B01D9/00 , B01D15/08 , B01D15/18 , B01D15/36 , B01D61/14 , B01D61/42 , B01D61/44 , C05B7/00 , C05C3/00 , C05D1/00 , C05D1/02 , C05D5/00 , C05F5/00 , C05F11/00 , C12P3/00 , C12P7/06 , C12P7/10 , C12P19/02 , C12P19/04 , C12P19/14
CPC分类号： B01D61/147 , B01B1/005 , B01D15/1821 , B01D15/365 , B01D61/44 , C01C1/28 , C01D3/04 , C01D5/04 , C01D5/16 , C05B7/00 , C05C3/00 , C05D1/00 , C05D1/02 , C05D5/00 , C05F11/00 , C12P3/00 , C12P7/06 , C12P7/10 , C12P19/02 , C12P2201/00 , Y02E50/16 , Y02E50/17
摘要： A method for recovering inorganic salt during processing of a lignocellulosic feedstock is provided. The method comprises pretreating the lignocellulosic feedstock by adding an acid to the feedstock to produce a pretreated lignocellulosic feedstock. A soluble base is then added to the pretreated lignocellulosic feedstock to adjust the pH and produce a neutralized feedstock. The neutralized feedstock is then enzymatically hydrolyzed to produce an enzyme hydrolyzed feedstock and a sugar stream. Inorganic salt is recovered from either a stream obtained from the lignocellulosic feedstock prior to the step of pretreating, a stream obtained from the pretreated lignocellulosic feedstock, a stream obtained from the neutralized feedstock, a stream obtained from the sugar stream, or a combination of these streams. The inorganic salt may be concentrated, clarified, recovered and purified by crystallization, electrodialysis drying, or agglomeration and granulation, and then used as desired, for example as a fertilizer.
摘要翻译：提供了在木质纤维素原料的加工过程中回收无机盐的方法。该方法包括通过向原料中加入酸来预处理木质纤维素原料以产生预处理的木质纤维素原料。然后将可溶碱添加到预处理的木质纤维素原料中以调节pH并产生中和的原料。然后将中和的原料酶水解以产生酶水解原料和糖流。在预处理步骤之前从木质纤维素原料获得的物流中回收无机盐，从预处理的木质纤维素原料获得的物流，从中和原料获得的物流，从糖流获得的物流，或这些物质的组合流。无机盐可以通过结晶，电渗析干燥或附聚和造粒进行浓缩，澄清，回收和纯化，然后根据需要使用，例如作为肥料。

5. 发明申请

US20070148751A1 Method of processing lignocellulosic feedstock for enhanced xylose and ethanol production 有权
标题翻译：处理木质纤维素原料以增强木糖和乙醇生产的方法
公开(公告)号：US20070148751A1
公开(公告)日：2007-06-28
申请号：US11700362
申请日：2007-01-31
申请人： Robert Griffin , Colin Nicholson , Corinne Mott , Jeffrey Tolan , VIjay Anand
发明人： Robert Griffin , Colin Nicholson , Corinne Mott , Jeffrey Tolan , VIjay Anand
IPC分类号： C12P7/06 , C12P19/02
CPC分类号： C13K1/02 , A23L33/21 , C12P7/10 , C12P19/02 , C12P19/14 , C12P2201/00 , Y02E50/16 , Y02E50/17
摘要： The present invention provides a method of producing xylose from lignocellulosic feedstock. The method comprises disrupting lignocellulosic feedstock; leaching the lignocellulosic feedstock by contacting the feedstock with at least one aqueous solution for a period greater than about 2 minutes to produce a leached feedstock and a leachate; removing the leachate from the leached feedstock; acidifying the leached feedstock to a pH between about 0.5 and about 3 to produce an acidified feedstock, and; reacting the acidified feedstock under conditions which disrupt fiber structure and hydrolyze a portion of hemicellulose and cellulose of the acidified feedstock, to produce a composition comprising xylose and a pretreated feedstock. The xylose may be purified from the pretreated feedstock or it may be converted to ethanol with the pretreated feedstock.
摘要翻译：本发明提供从木质纤维素原料生产木糖的方法。该方法包括破坏木质纤维素原料; 通过使原料与至少一种水溶液接触大于约2分钟的时间来浸出木质纤维素原料以产生浸出的原料和浸出液; 从浸出的原料中除去渗滤液; 将浸出的原料酸化至约0.5至约3之间的pH以产生酸化原料; 以及在破坏纤维结构并水解酸化原料的半纤维素和纤维素的一部分的条件下使酸化的原料反应，以产生包含木糖和经预处理的原料的组合物。木糖可以从预处理的原料中纯化，或者可以用预处理的原料转化成乙醇。

6. 发明授权

US5591304A Method for the use of enzymes in bleaching paper pulp 失效
标题翻译：在漂白纸浆中使用酶的方法
公开(公告)号：US5591304A
公开(公告)日：1997-01-07
申请号：US317828
申请日：1994-10-04
申请人： Jeffrey Tolan , Brian Foody
发明人： Jeffrey Tolan , Brian Foody
IPC分类号： C12S3/08 , D21C20060101 , D21C3/02 , D21C5/00 , D21C9/02 , D21C9/10 , D21F9/00
CPC分类号： D21C5/005 , D21C9/1005
摘要： A method and apparatus for treating wood pulp that includes incompletely washed brownstock, in which the brownstock is treated at a pH range of approximately 7.0 to 9.0 with a hemicellulase enzyme preparation that has a pH optimum below 6.0. Also, a method and apparatus for treating wood pulp containing incompletely washed brownstock in which the brownstock is treated at a pH range of approximately 6.0 to 9.0 with a hemicellulase enzyme preparation that has a pH optimum below 6.0 and that has a low cellulase content such that not more than about 10,000 FPU are added per ton of pulp.
摘要翻译：一种用于处理木浆的方法和设备，其包括不完全洗涤的粗浆，其中在约7.0至9.0的pH范围内处理棕色浆料，其中pH最佳低于6.0的半纤维素酶制剂。另外，一种用于处理含有不完全洗涤的粗浆的木浆的方法和装置，其中在约6.0至9.0的pH范围内处理褐色原液，其中半纤维素酶制剂的pH最佳值低于6.0，并且具有低的纤维素酶含量，使得每吨纸浆不得超过约10,000 FPU。

7. 发明授权

US08318461B2 Enzyme compositions for the improved enzymatic hydrolysis of cellulose and methods of using same 失效
标题翻译：用于改进纤维素酶水解的酶组合物及其使用方法
公开(公告)号：US08318461B2
公开(公告)日：2012-11-27
申请号：US12305110
申请日：2007-06-22
申请人： Jeffrey Tolan , Theresa White , John Tomashek
发明人： Jeffrey Tolan , Theresa White , John Tomashek
IPC分类号： C12P19/02 , C12N9/42 , C12N9/38
CPC分类号： C12N9/2437 , C12N9/2445 , C12P7/10 , C12P19/02 , C12Y302/01021 , Y02E50/16 , Y02P20/582
摘要： A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one β-glucosidase enzyme and a binding agent for binding the β-glucosidase enzyme to fiber solids present in the aqueous slurry. During the hydrolysis, both the cellulase enzyme and β-glucosidase enzyme bind to the fiber solids. The hydrolysis is performed in a solids-retaining hydrolysis reactor so that unhydrolyzed fiber solids and bound enzyme are retained in the reactor longer than the aqueous phase of the slurry.
摘要翻译：提供了纤维素酶水解以产生包含来自预处理的木质纤维素原料的葡萄糖和用于该方法的酶的水解产物的方法。该方法包括用纤维素酶，一种或多于一种葡糖苷酶和用于将葡糖苷酶结合到含水浆料中的纤维固体的结合剂来水解预处理的木质纤维素原料的含水浆料。在水解期间，纤维素酶和β-葡糖苷酶都结合纤维固体。水解在固体保持水解反应器中进行，使得未水解的纤维固体和结合的酶比浆液的水相保留在反应器中。

8. 发明申请

US20100068768A1 ENZYME COMPOSITIONS AND METHODS FOR THE IMPROVED ENZYMATIC HYDROLYSIS OF CELLULOSE 失效
标题翻译：用于改善纤维素酶水解的酶组合物和方法
公开(公告)号：US20100068768A1
公开(公告)日：2010-03-18
申请号：US12305069
申请日：2007-06-22
申请人： Jeffrey Tolan , Theresa White , John Tomashek
发明人： Jeffrey Tolan , Theresa White , John Tomashek
IPC分类号： C12P19/14 , C12N9/42
CPC分类号： C12N9/2437 , C12N9/2445 , C12P7/10 , C12P19/02 , C12Y302/01021 , Y02E50/16 , Y02P20/582
摘要： A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises partially hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one β-glucosidase enzyme and a binding agent for binding the β-glucosidase enzyme to fiber solids present in the aqueous slurry. The unhydrolyzed fiber solids are then separated from the hydrolyzed slurry. The separated fiber solids thus obtained are then re-suspended in an aqueous solution to produce a re-suspended slurry. The hydrolysis is then continued to produce the hydrolysis product comprising glucose.
摘要翻译：提供了纤维素酶水解以产生包含来自预处理的木质纤维素原料的葡萄糖和用于该方法的酶的水解产物的方法。该方法包括用纤维素酶，一种或多于一种葡糖苷酶和用于将葡糖苷酶与含水浆料中存在的纤维固体结合的结合剂部分水解预处理的木质纤维素原料的含水浆料。然后将未水解的纤维固体与水解浆料分离。然后将由此获得的分离的纤维固体重新悬浮在水溶液中以产生再悬浮的浆料。然后继续水解以产生包含葡萄糖的水解产物。

9. 发明申请

US20090209009A1 ENZYME COMPOSITIONS FOR THE IMPROVED ENZYMATIC HYDROLYSIS OF CELLULOSE AND METHODS OF USING SAME 失效
标题翻译：用于改善纤维素酶水解的酶组合物及其使用方法
公开(公告)号：US20090209009A1
公开(公告)日：2009-08-20
申请号：US12305110
申请日：2007-06-22
申请人： Jeffrey Tolan , Theresa White , John Tomashek
发明人： Jeffrey Tolan , Theresa White , John Tomashek
IPC分类号： C12P19/02 , C12N9/42 , C12N9/38
CPC分类号： C12N9/2437 , C12N9/2445 , C12P7/10 , C12P19/02 , C12Y302/01021 , Y02E50/16 , Y02P20/582
摘要： A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one β-glucosidase enzyme and a binding agent for binding the β-glucosidase enzyme to fiber solids present in the aqueous slurry. During the hydrolysis, both the cellulase enzyme and β-glucosidase enzyme bind to the fiber solids. The hydrolysis is performed in a solids-retaining hydrolysis reactor so that unhydrolyzed fiber solids and bound enzyme are retained in the reactor longer than the aqueous phase of the slurry.
摘要翻译：提供了纤维素酶水解以产生包含来自预处理的木质纤维素原料的葡萄糖和用于该方法的酶的水解产物的方法。该方法包括用纤维素酶，一种或多于一种β-葡糖苷酶和用于将β-葡糖苷酶与含水浆料中存在的纤维固体结合的结合剂来水解预处理的木质纤维素原料的含水浆料。在水解期间，纤维素酶和β-葡糖苷酶都结合纤维固体。水解在固体保持水解反应器中进行，使得未水解的纤维固体和结合的酶比浆液的水相保留在反应器中。

10. 发明申请

US20060008885A1 Method for glucose production using endoglucanase core protein for improved recovery and reuse of enzyme 有权
标题翻译：使用内切葡聚糖酶核心蛋白进行葡萄糖生产的方法用于改善酶的回收和再利用
公开(公告)号：US20060008885A1
公开(公告)日：2006-01-12
申请号：US10507618
申请日：2003-03-05
申请人： Daphne Wahnon , Theresa White , Jennifer Donaldson , Jeffrey Tolan
发明人： Daphne Wahnon , Theresa White , Jennifer Donaldson , Jeffrey Tolan
IPC分类号： C12P19/02 , C12P7/06 , C12N9/24
CPC分类号： C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present invention pertains to a method of converting cellulose to glucose by treating a pretreated lignocellulosic substrate with an enzyme mixture comprising cellulase enzyme and endoglucanase core proteins, wherein the endoglucanase core proteins are present in the enzyme mixture at an amount relative to all endoglucanases from about 35 wt. % to about 100 wt. % and wherein the endoglucanase cellulase enzymes are present in the enzyme mixture at an amount relative to the amount of CBH and EG enzymes from about 2 wt. % to about 50 wt. %. The pretreated lignocellulosic substrate is selected from the group consisting of agricultural residues, residues after starch or sugar removal, dedicated ethanol crops, forestry products, and pulp and paper products, or combinations thereof.
摘要翻译：本发明涉及通过用包含纤维素酶和内切葡聚糖酶核心蛋白质的酶混合物处理预处理的木质纤维素底物来将纤维素转化为葡萄糖的方法，其中内切葡聚糖酶核心蛋白以相对于所有内切葡聚糖酶的量存在于酶混合物中 35重量％％至约100重量％％，其中内切葡聚糖酶纤维素酶以相对于CBH和EG酶的量约2重量％的量存在于酶混合物中。％至约50wt。％。预处理的木质纤维素底物选自农业残留物，淀粉或糖去除后的残留物，专用乙醇作物，林产品和纸浆和纸制品，或其组合。

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