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1. 发明授权

US5851770A Detection of mismatches by resolvase cleavage using a magnetic bead support 失效
标题翻译：使用磁珠支撑通过解析酶裂解检测错配
公开(公告)号：US5851770A
公开(公告)日：1998-12-22
申请号：US545404
申请日：1995-10-19
申请人： Jeff Babon , Rima Youil , Jay Stoerker , Anne Huff , Richard G. H. Cotton
发明人： Jeff Babon , Rima Youil , Jay Stoerker , Anne Huff , Richard G. H. Cotton
IPC分类号： C12N15/09 , C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6888 , C12Q2600/156
摘要： Disclosed is a method for detecting one or more mismatches between a first and a second nucleic acid, the first and second nucleic acids being capable of preferentially hybridizing. The method involves: a) providing the first nucleic acid in its single-stranded form, the first nucleic acid being bound to the first member of a specific binding pair; b) providing the second nucleic acid in its single-stranded form, the second nucleic acid being bound to a detectably labelled reagent; c) contacting the first nucleic acid with the second nucleic acid under conditions allowing heteroduplex formation; d) contacting the product of step (c) with a magnetic bead to which is bound the second member of the specific binding pair under conditions allowing complex formation between the first and the second members of the specific binding pair; e) applying a magnetic field to the mixture to facilitate separation of the magnetic bead from the remainder of the product of step (c); f) contacting the magnetic bead-bound nucleic acid with a resolvase capable of recognizing at least one single base pair mismatch in a heteroduplex, under conditions which permit the resolvase to cleave the heteroduplex; and f) analyzing the product of step (f), the presence of a cleavage product being an indication of a mismatch between the first and second nucleic acids.
摘要翻译：公开了一种用于检测第一和第二核酸之间的一个或多个错配的方法，所述第一和第二核酸能够优先杂交。该方法包括：a）提供其单链形式的第一核酸，第一核酸与特异性结合对的第一个成员结合; b）提供其单链形式的第二核酸，第二核酸与可检测标记的试剂结合; c）在允许异源双链体形成的条件下使第一核酸与第二核酸接触; d）使步骤（c）的产物与限制特异性结合对的第二个成员的磁珠在允许特异性结合对的第一和第二成员之间形成复合物的条件下接触; e）向所述混合物施加磁场以促进磁珠与步骤（c）的产物的其余部分分离; f）使所述磁珠结合的核酸与能够在异源双链体中识别至少一个单碱基对失配的解淀粉酶在允许所述分解酶切割所述异源双链体的条件下接触; 和f）分析步骤（f）的产物，裂解产物的存在是第一和第二核酸之间错配的指示。

2. 发明申请

US20060068434A1 Methods and compositions for detecting cancer using components of the U2 spliceosomal particle 审中-公开
标题翻译：使用U2剪接体颗粒的成分检测癌症的方法和组合物
公开(公告)号：US20060068434A1
公开(公告)日：2006-03-30
申请号：US11232440
申请日：2005-09-21
申请人： Jay Stoerker
发明人： Jay Stoerker
IPC分类号： C12Q1/68 , G01N33/574
CPC分类号： G01N33/57415 , G01N33/574 , G01N33/57488
摘要： The present invention relates to cancer-associated proteins and nucleic acids that encode or bind specifically to cancer-associated proteins, which represent markers for cancer detection. Specifically, the invention provides a family of methods and compositions for detecting cancer, for example, breast cancer, in an individual using components of the U2 spliceosomal particle. A target cancer-associated protein may be detected, for example, by reacting the sample with a labeled binding moiety, for example, a labeled antibody capable of binding specifically to the protein. The invention also provides kits useful in the detection of cancer in an individual.
摘要翻译：本发明涉及编码或特异性结合癌症相关蛋白的癌相关蛋白和核酸，其代表癌症检测的标志物。具体地，本发明提供了一种用于使用U2剪接体颗粒的组分在个体中检测癌症例如乳腺癌的方法和组合物族。可以例如通过使样品与标记结合部分反应，例如能够特异性结合蛋白质的标记抗体来检测靶癌相关蛋白。本发明还提供了可用于检测个体中的癌症的试剂盒。

3. 发明授权

US5843649A Method of identifying clonal cell samples using heteroduplex generators 失效
标题翻译：使用异源双极发生器识别克隆细胞样品的方法
公开(公告)号：US5843649A
公开(公告)日：1998-12-01
申请号：US396927
申请日：1995-03-01
申请人： Jay Stoerker , Kenneth R. Shroyer
发明人： Jay Stoerker , Kenneth R. Shroyer
IPC分类号： C12Q1/68 , C07H21/04 , C12P19/34
CPC分类号： C12Q1/6886 , C12Q1/6827 , C12Q2600/112 , C12Q2600/154
摘要： Methods of distinguishing between neoplasms and pseudoneoplastic or hyperplastic processes are disclosed. The methods comprise first contacting DNA from cells of a tissue sample from a female individual with a cytosine-methylation specific endonuclease, generating amplified fragments of a cytosine-methylation regulatable polymorphic X chromosome gene using primers that bracket a restriction site of said cytosine-methylation specific endonuclease and a polymorphic sequence in said nucleotide sequence of the gene; generating heteroduplexes between amplified fragments and heteroduplex generators which consist of 1-5 nucleotide differences from the amplified fragments; and detecting the presence of a single species of heteroduplex or two species of heteroduplexes. The presence of a single species of heteroduplex which indicates amplified fragments from a clonal population of cells which is indicative a neoplasm and the presence of two species of heteroduplexes indicates amplified fragments from a mosaic population of cells is indicative a pseudoneoplastic or hyperplastic process. Reagents and kits for performing the methods are disclosed.
摘要翻译：公开了区分肿瘤和假性增生或增生过程的方法。所述方法包括首先将来自雌性个体的组织样品的细胞的DNA与胞嘧啶 - 甲基化特异性内切核酸酶接触，使用包含所述胞嘧啶甲基化特异性的限制性位点的引物产生胞嘧啶甲基化可调节多态性X染色体基因的扩增片段核酸内切酶和所述基因的所述核苷酸序列中的多态性序列; 在扩增片段和异源双链产生子之间产生异源双链体，其由与扩增片段的1-5个核苷酸差异组成; 并检测单种异源双链体或两种异源双链体的存在。单种异源双链体的存在，其表示来自指示肿瘤的细胞的克隆群体的扩增片段和两种异源双链体的存在表明来自细胞镶嵌群体的扩增片段指示假性增生或增生过程。公开了用于执行方法的试剂和试剂盒。

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